Abstract:
:L-Ribose is an important precursor for antiviral agents, and thus its high-level production is urgently demanded. For this aim, immobilized recombinant Escherichia coli cells expressing the L-arabinose isomerase and variant mannose-6-phosphate isomerase genes from Geobacillus thermodenitrificans were developed. The immobilized cells produced 99 g/l L-ribose from 300 g/l L-arabinose in 3 h at pH 7.5 and 60 °C in the presence of 1 mM Co(2+), with a conversion yield of 33 % (w/w) and a productivity of 33 g/l/h. The immobilized cells in the packed-bed bioreactor at a dilution rate of 0.2 h(-1) produced an average of 100 g/l L-ribose with a conversion yield of 33 % and a productivity of 5.0 g/l/h for the first 12 days, and the operational half-life in the bioreactor was 28 days. Our study is first verification for L-ribose production by long-term operation and feasible for cost-effective commercialization. The immobilized cells in the present study also showed the highest conversion yield among processes from L-arabinose as the substrate.
journal_name
Appl Biochem Biotechnoljournal_title
Applied biochemistry and biotechnologyauthors
Kim KR,Seo ES,Oh DKdoi
10.1007/s12010-013-0547-xsubject
Has Abstractpub_date
2014-01-01 00:00:00pages
275-88issue
1eissn
0273-2289issn
1559-0291journal_volume
172pub_type
杂志文章abstract::The metabolic engineering of Chlamydomonas reinhardtii, one of the fastest-growing microalgae, is a potential alternative for enhanced carotenoid productivity. CrtYB (phytoene-β-carotene synthase - PBS) gene from red yeast Xanthophyllomyces dendrorhous encodes for a bifunctional enzyme that harbours both phytoene synt...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-019-03194-9
更新日期:2020-04-01 00:00:00
abstract::Corn fiber is the fibrous by-product of wet-mill corn processing. It typically consists of about 20% starch, 14% cellulose, and 30% hemicellulose in the form of arabinoxylan. Crude corn fiber (CCF) was fractionated into de-starched corn fiber (DSCF), corn fiber with cellulose (CFC) enriched, and corn fiber arabinoxyla...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:
更新日期:2005-04-01 00:00:00
abstract::Phytochelatin synthase (PCS) is a key enzyme involved in the synthesis of phytochelatins, which are thought to play important roles in heavy metal detoxification. The sacred lotus (Nelumbo nucifera), one of the most popular ornamental species, has been shown to be a potential phytoremediator of heavy metal polluted wa...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-011-9461-2
更新日期:2012-02-01 00:00:00
abstract::The aim of the present study was bioremediation of distillery sludge into a soil-enriching material. It was mixed with a complementary waste, cattle dung, and subjected to vermicomposting with (V) and without (T, control) Eisenia fetida in the ratio of 0:100 % (V1, T1), 10:90 (V2, T2), 25:75 (V3, T3), 50:50 (V4, T4), ...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-014-1116-7
更新日期:2014-10-01 00:00:00
abstract::A bench scale experimental system was developed for the analysis of polycyclic aromatic hydrocarbon (PAH) degradation by mixed microbial cultures in PAH contaminated Manufactured Gas Plant (MGP) soils and on sand. The reactor system was chosen in order to provide a fundamental protocol capable for evaluating the perfo...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/BF02922585
更新日期:1991-04-01 00:00:00
abstract::An aspartic protease gene from Pleurotus ostreatus (Po-Asp) had been cloned based on the 3' portion of cDNA in our previous work. The Po-Asp cDNA contained 1,324 nucleotides with an open reading frame (ORF) of 1,212 bp encoding 403 amino acid residues. The putative amino acid sequence included a signal peptide, an act...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0674-4
更新日期:2014-02-01 00:00:00
abstract::Ethanol production was studied in simultaneous saccharification and fermentation (SSF) of steam-pretreated spruce at 42 degrees C, using a thermotolerant yeast. Three yeast strains of Kluyveromyces marxianus were compared in test fermentations. SSF experiments were performed with the best of these on 5% (w/w) of subst...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1385/abab:84-86:1-9:69
更新日期:2000-04-01 00:00:00
abstract::In this study, Nostoc muscorum, a native cyanobacterial species isolated from a coal mining site, was employed to remove Cu(II), Zn(II), Pb(II) and Cd(II) from aqueous solution containing these metals in the mixture. In this multicomponent study, carried out as per the statistically valid Plackett-Burman design of exp...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-015-1553-y
更新日期:2015-04-01 00:00:00
abstract::A full factorial design leading to 20 sets of sorption runs was conducted to study the influence of four variables (bleaching earth and biomass concentrations, pH, and sorption time) on the iron, nickel, and chromium removal from stainless steel effluent using waste biomass from a beverage industry. Similar factor eff...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1385/abab:91-93:1-9:413
更新日期:2001-04-01 00:00:00
abstract::In this study, an easily detectable method was employed for screening laccase-producing microorganisms by using 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) as laccase secretion indicator. A novel laccase-producing strain was isolated and identified as Shiraia bambusicola Henn. strain GZ11K2 according to the mor...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-012-9943-x
更新日期:2012-12-01 00:00:00
abstract::Several recombinant derivatives of serine protease inhibitor called silk protease inhibitor 2 (SPI2), which is a silk component in Galleria mellonella (Lepidoptera, Insecta), were prepared in the expression vector Pichia pastoris. Both the native and the recombinant protease inhibitors were highly active against subti...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0325-9
更新日期:2013-09-01 00:00:00
abstract::Aspergillus niger phytase (PhyA) has been used as a feed supplement to improve the bioavailability of phytate phosphorus to swine and poultry. However, it is unable to maintain its stability due to high temperature during the feed pelleting process. In this study, we performed site-directed mutagenesis in the Aspergil...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0380-2
更新日期:2013-10-01 00:00:00
abstract::Biosorption is a potential tool for the extraction of metals from contaminated water and recovery of precious metals, which is a convenient alternative to conventional processes. In the present study, molybdenum recovery by Acidithiobacillus ferrooxidans strain ZT-94 was evaluated. Additionally, the effects of pH init...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-020-03468-7
更新日期:2020-11-17 00:00:00
abstract::Genetic polymorphism of the cytochrome P450 (CYP) genes particularly affects CYP2D6 and CYP2C19 to a functionally relevant extent, and it is therefore crucial to elucidate the enzyme kinetic and molecular basis for altered catalytic activity of these allelic variants. This study explored the expression and function of...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-018-2728-0
更新日期:2018-09-01 00:00:00
abstract::The potential market for lactic acid as the feedstock for biodegradable polymers, oxygenated chemicals, and specialty chemicals is significant. L-lactic acid is often the desired enantiomer for such applications. However, stereospecific lactobacilli do not metabolize starch efficiently. In this work, Argonne researche...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/BF02920157
更新日期:1998-04-01 00:00:00
abstract::An extracellular thermostable xylanase from a newly isolated thermophilic Actinomadura sp. strain Cpt20 was purified and characterized. Based on matrix-assisted laser desorption-ionization time-of-flight mass spectrometry analysis, the purified enzyme is a monomer with a molecular mass of 20,110.13 Da. The 19 residue ...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-011-9457-y
更新日期:2012-02-01 00:00:00
abstract::Fresh-harvested, air-dried rice straw was pretreated at a water content of 5 g H(2)O/g straw using sodium hydroxide (NaOH) and compared to pretreatment at 10 g H(2)O/g straw by hydrated lime (Ca(OH)(2)). Full factorial experiments including parallel wash-only treatments were completed with both sources of alkali. The ...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-010-8958-4
更新日期:2010-11-01 00:00:00
abstract::Fatty acid methyl esters (FAMEs) show large potential applications as diesel substitutes, also known as biodiesel fuel. Biodiesel fuel as renewable energy is an alternative that can reduce energy dependence on petroleum as well as air pollution. Several processes for the production of biodiesel fuel have been develope...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1385/abab:114:1-3:747
更新日期:2004-04-01 00:00:00
abstract::In this study, the prokaryotic expression system of Escherichia coli was used to modify prolyl aminopeptidase derived from Aspergillus oryzae JN-412 (AoPAP) via random mutagenesis and site-directed saturation mutagenesis. A random mutant library with a capacity of approximately 3000 mutants was compiled using error-pr...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-020-03277-y
更新日期:2020-08-01 00:00:00
abstract::Marine Aspergillus awamori BTMFW032, recently reported by us, produce acidophilic tannase as extracellular enzyme. Here, we report the application of this enzyme for synthesis of propyl gallate by direct transesterification of tannic acid and in tea cream solubilisation besides the simultaneous production of gallic ac...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-011-9162-x
更新日期:2011-07-01 00:00:00
abstract::Pseudomonas are ubiquitous bacteria that can live under a wide range of environmental conditions; some species are successfully used in the fermentation industry. Pseudomonas plecoglossicida JUIM01 strain is an important industrial 2-ketogluconate (2KGA) producer. In Pseudomonas, kguT gene encodes a putative 2KGA tran...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-018-2843-y
更新日期:2019-03-01 00:00:00
abstract::The oil palm sector is one of the major plantation industries in Malaysia. Palm kernel cake is a byproduct of extracted palm kernel oil. Mostly palm kernel cake is wasted or is mixed with other nutrients and used as animal feed, especially for ruminant animals. Recently, palm kernel cake has been identified as an impo...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1385/abab:118:1-3:073
更新日期:2004-07-01 00:00:00
abstract::The present study deals with the characterization of halotolerant protease produced by Bacillus aquimaris VITP4 strain isolated from Kumta coast, Karnataka, India. The studies were performed at 40 °C and pH 8 in Tris buffer. Metal ions such as Mn(2+) and Ca(2+) increased the proteolytic activity of the enzyme by 34 an...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0524-4
更新日期:2014-01-01 00:00:00
abstract::A new colorimetric chemosensor for naked-eye detection and determination of cysteine (Cys) based on indicator displacement assay (IDA) was designed using 1-(2-pyridylazo)-2-naphthol (PAN). The indicator exchange occurred between PAN and Cys by the addition of Cys to the Cu(PAN)2 complex, which is accomplished by an im...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-019-03165-0
更新日期:2020-09-01 00:00:00
abstract::To determine the influence of various culture conditions, transformed cells of Escherichia coli expressing recombinant green fluorescent protein (GFPuv) were grown in nine cultures with four variable conditions (storage of inoculated broth at 4 degrees C prior to incubation, agitation speed, isopropyl-beta-D-thiogalac...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1385/abab:114:1-3:453
更新日期:2004-04-01 00:00:00
abstract::Glial cell line-derived neurotrophic factor (GDNF) is a type of neurotrophic factor with significant potential in treatment of Parkinson's disease. Combining gene targeting of animal somatic cells with nuclear transfer technique has provided a powerful method to produce transgenic animal mammary gland bioreactor. The ...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-009-8545-8
更新日期:2009-12-01 00:00:00
abstract::A multi-method protocol previously proposed for the extraction of extracellular polymeric substances (EPS) from flocculated sludges was investigated on dense aerobic granules. The protocol combines mechanical disruption by sonication and chemical extraction using the Tween detergent and the cation chelator, EDTA. Poly...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-012-9569-z
更新日期:2012-04-01 00:00:00
abstract::Cellulases can be used for biofuel production to decrease the fuel crises in the world. Microorganisms cultured on lignocellulosic wastes can be used for the production of cellulolytic enzymes at large scale. In the current study, cellulolytic enzyme production potential of Aspergillus fumigatus was explored and optim...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-014-0796-3
更新日期:2014-04-01 00:00:00
abstract::Agar-immobilized alkaliphilic Bacillus sp. AR-009 cells were used for xylanase production using batch and continuous culture. In a batch culture, maximum enzyme production was observed after 48 h and remained high up to 72 h. In repeated batch cultivation, immobilized cells produced an appreciable level of xylanase ac...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1385/abab:87:2:95
更新日期:2000-05-01 00:00:00
abstract::A Bacillus subtilis strain isolated from contaminated soil from a refinery has been screened for biosurfactant production in crystal sugar (sucrose) with different nitrogen sources (NaNO(3), (NH(4))(2)SO(4), urea, and residual brewery yeast). The highest reduction in surface tension was achieved with a 48-h fermentati...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-007-9073-z
更新日期:2007-04-01 00:00:00