Enzymatic Characterization of Fructose 1,6-Bisphosphatase II from Francisella tularensis, an Essential Enzyme for Pathogenesis.

abstract：:The bienzyme electrodes were fabricated by coimmobilization of lactate oxidase (LOD) and lactate dehydrogenase (LDH) onto electrochemically prepared polyaniline (PANI) films. These PANI/LOD/LDH bienzyme electrodes were shown to provide signal amplification by substrate recycling, making it possible to detect L-lactate...

journal_title：Applied biochemistry and biotechnology

authors： Chaubey A,Pande KK,Pandey MK,Singh VS

更新日期：2001-10-01 00:00:00

abstract：:Proteins that are important indicators of physiological or pathological states may contribute to the early diagnosis of disease, which may provide a basis for identifying the underlying mechanism of disease development. Serum, contains an abundance of proteins, offers an easy and inexpensive approach for disease detec...

journal_title：Applied biochemistry and biotechnology

authors： Zhang AH,Sun H,Yan GL,Han Y,Wang XJ

更新日期：2013-06-01 00:00:00

abstract：:A comparative analysis of the structural and functional aspects along with equilibrium unfolding of two homologous cutinases, Cut1 and Cut2, from Thermobifida fusca was carried out. The CD and fluorescence profile at different pH in the range of 6 to 9 showed no structural variations for both cutinases, indicating the...

journal_title：Applied biochemistry and biotechnology

authors： Hegde K,Dasu VV

更新日期：2014-09-01 00:00:00

abstract：:Streptomyces griseorubens JSD-1 is a novel actinomycete that could grow efficiently upon lignin, and the ligninolytic genes active in this biotransformation were expected to be crucial. To investigate the molecular mechanism of utilizing lignin, genome sequencing was carried out to obtain its draft genome, which was d...

journal_title：Applied biochemistry and biotechnology

authors： Feng H,Zhang D,Sun Y,Zhi Y,Mao L,Luo Y,Xu L,Wang L,Zhou P

更新日期：2015-05-01 00:00:00

abstract：:The partition behavior of proteins, nucleic acids, cell membranes, cell organelles and whole cells has been studied in liquid-liquid two-phase systems composed of water, poly-(ethylene glycol), and an hydroxypropyl starch. The properties of the systems are in many respects comparable with the traditional poly(ethylene...

journal_title：Applied biochemistry and biotechnology

authors： Sturesson S,Tjerneld F,Johansson G

更新日期：1990-12-01 00:00:00

abstract：:Tomato pomace is an abundant lignocellulosic waste stream from industrial tomato processing and therefore a potential feedstock for production of renewable biofuels. However, little research has been conducted to determine if pretreatment can enhance release of fermentable sugars from tomato pomace. Ionic liquids (ILs...

journal_title：Applied biochemistry and biotechnology

authors： Allison BJ,Cádiz JC,Karuna N,Jeoh T,Simmons CW

更新日期：2016-08-01 00:00:00

abstract：:Fibrobacter succinogenes is a cellulolytic bacterium that degrades plant cell wall biomass in ruminant animals and is among the most rapidly fibrolytic of all mesophilic bacteria. The complete genome sequence of Fisuc was completed by the DOE Joint Genome Institute in late 2009. Using new expression tools developed at...

journal_title：Applied biochemistry and biotechnology

authors： Brumm P,Mead D,Boyum J,Drinkwater C,Deneke J,Gowda K,Stevenson D,Weimer P

更新日期：2011-03-01 00:00:00

abstract：:Glial cell line-derived neurotrophic factor (GDNF) is a type of neurotrophic factor with significant potential in treatment of Parkinson's disease. Combining gene targeting of animal somatic cells with nuclear transfer technique has provided a powerful method to produce transgenic animal mammary gland bioreactor. The ...

journal_title：Applied biochemistry and biotechnology

authors： Zhang X,Wu Y,Luo F,Su H,Bai Y,Hou Y,Yu B

更新日期：2009-12-01 00:00:00

abstract：:The bacterium Enterobacter A47 has demonstrated the ability to synthesise distinct exopolysaccharides (EPS) as a function of the substrate used. The culture's performance was evaluated in experiments using either glucose or xylose, as single carbon sources, and compared with the substrate (glycerol) used in previous s...

journal_title：Applied biochemistry and biotechnology

authors： Freitas F,Alves VD,Gouveia AR,Pinheiro C,Torres CA,Grandfils C,Reis MA

更新日期：2014-01-01 00:00:00

abstract：:Bacterial strain Vibrio sp. (PIGB 184) isolated from water samples of the Arabian Sea and identified through 16S rRNA demonstrated the production of pigmentary antioxidants with higher ABTS activities 90.9 ± 0.42 % in comparison with the standard commercial pigmented antioxidant, quercetin 88.8 ± 1.4 %. Antioxidative ...

journal_title：Applied biochemistry and biotechnology

authors： Pawar R,Mohandass C,Dastager SG,Kolekar YM,Malwankar R

更新日期：2016-04-01 00:00:00

abstract：:Lignocellulose biomass contain large macromolecules especially cellulose and hemicelluloses that can be converted to fuel and chemicals using microbial biocatalysts. This study presents comprehensive optimization of production of biomass-hydrolyzing enzymes (BHE) by a high β-glucosidase-producing Trichoderma SG2 for b...

journal_title：Applied biochemistry and biotechnology

authors： Nanjundaswamy A,Okeke BC

更新日期：2020-05-01 00:00:00

abstract：:A new, simple, and original method is described for specific measurement of polyunsaturated fatty acid content in olive oil. This analytical system uses coupled enzymes, lipase and lipoxygenase. The system consists of lipase-catalyzed hydrolysis of triacylglycerol and subsequent lipoxygenation of liberated polyunsatur...

journal_title：Applied biochemistry and biotechnology

authors： Ben Rejeb I,Monser L,Gargouri M

更新日期：2010-11-01 00:00:00

abstract：:This study represents the development of a new batch method by consortium GG-BL using two microbial cultures viz., Galactomyces geotrichum MTCC 1360 and Brevibacillus laterosporus MTCC 2298, by varying environmental conditions for the decolorization and biodegradation of Rubine GFL. Consortium was found to give better...

journal_title：Applied biochemistry and biotechnology

authors： Waghmode TR,Kurade MB,Lade HS,Govindwar SP

更新日期：2012-07-01 00:00:00

abstract：:Recombinant tissue-plasminogen activator (r-tPA), expressed in Escherichia coli cells in an aggregated form, was solubilized with a strong chaotrope in the absence of any reducing agent. The solubilized molecule was reactivated by a procedure that was developed to mimic the physiological conditions optimal for the fun...

journal_title：Applied biochemistry and biotechnology

authors： Grunfeld H,Patel A,Shatzman A,Nishikawa AH

更新日期：1992-05-01 00:00:00

abstract：:Soaking in aqueous ammonia (SAA) pretreatment was investigated to improve enzymatic digestibility and consequently to increase total fermentable sugar production from barley straw. Various effects of pretreatment process parameters, such as reaction temperature, reaction time, solid:liquid ratio, and ammonia concentra...

journal_title：Applied biochemistry and biotechnology

authors： Yoo CG,Nghiem NP,Hicks KB,Kim TH

更新日期：2013-04-01 00:00:00

abstract：:Oxidation of lignin obtained from acetosolv and ethanol/water pulping of sugarcane bagasse was performed by phenol oxidases: tyrosinase (TYR) and laccase (LAC), to increase the number of carbonyl and hydroxyl groups in lignin, and to improve its chelating capacity. The chelating properties of the original and oxidized...

journal_title：Applied biochemistry and biotechnology

authors： Calabria GM,Gonçalves AR

更新日期：2006-04-01 00:00:00

abstract：:Lignocellulose-decaying fungal bioplatforms available are not commercially accessible and are limited to short-term use. In this study, those limitations were overcome by developing a platform using water-soaked rice straw (RS) biodegraded by irradiation-based fungal pretreatment (IBFP). This eco-friendly system incre...

journal_title：Applied biochemistry and biotechnology

authors： Bak JS

更新日期：2015-12-01 00:00:00

abstract：:This research aimed at developing and designing a slow and targeted delivery of Amphotericin B (AmB) antibiotic by placing three types of shells containing different ratios of biodegradable and biocompatible polymers poly (D, L-lactide)-co-(glycolide) (PLGA), polyethylene glycol (PEG), and polyvinyl pyrrolidone (PVP) ...

journal_title：Applied biochemistry and biotechnology

authors： Sadat Akhavi S,Moradi Dehaghi S

更新日期：2020-06-01 00:00:00

abstract：:Reed plants (Phragmites communis (Linn.) Trin) are hydrophilic perennial grasses growing in fresh and brackish waters. These plants readily adapt to arid and high salinity conditions; however, their resistance mechanism against abiotic stresses, especially high salinity, is largely unknown. In the present study, we cl...

journal_title：Applied biochemistry and biotechnology

authors： Zhang X,Quan G,Wang J,Han H,Chen S,Guo S,Yin H

更新日期：2015-04-01 00:00:00

abstract：:The short supply of edible vegetable oils is the limiting factor in the progression of biodiesel technology; thus, in this study, we applied response surface methodology in order to optimize the reaction factors for biodiesel synthesis from inedible castor oil. Specifically, we evaluated the effects of multiple parame...

journal_title：Applied biochemistry and biotechnology

authors： Jeong GT,Park DH

更新日期：2009-05-01 00:00:00

abstract：:Forty-four endophytic fungal isolates obtained from marine sponge, Hyrtios erectus, were evaluated and screened for their hydrolase activities. Most of the isolates were found to be prolific producers of hydrolytic enzymes. Only 11 isolates exhibited maximum cellular contents of lipids, rhamnolipids, and protein in th...

journal_title：Applied biochemistry and biotechnology

authors： El-Gendy MMAA,Yahya SMM,Hamed AR,Soltan MM,El-Bondkly AMA

更新日期：2018-07-01 00:00:00

abstract：:Gluconacetobacter spp. synthesize a pure form of hydrophilic cellulose that has several industrial specialty applications. Literature reports have concentrated on intensive investigation of static and agitated culture in liquid media containing high nutrient concentrations optimized for maximal cellulose production ra...

journal_title：Applied biochemistry and biotechnology

authors： Evans BR,O'Neill HM

更新日期：2005-04-01 00:00:00

abstract：:The use of probiotics, alone or in interaction with bile acids, is a modern strategy in the prevention and treatment of hypercholesterolemia. Numerous mechanisms for hypocholesterolemic effect of probiotics have been hypothesized, based mostly on in vitro evidence. Interaction with bile acids through reaction of decon...

journal_title：Applied biochemistry and biotechnology

authors： Pavlović N,Stankov K,Mikov M

更新日期：2012-12-01 00:00:00

abstract：:An animal protein-free medium composed of IPL-41 containing 6 g L(-1) yeastolate ultrafiltrate, 10 g L(-1) glucose, 2 g L(-1) lactose, 5 g L(-1) glutamine, 1% lipid emulsion, and 0.1% Pluronic F-68 was used for producing recombinant proteins in batch mode employing two cell lines, S2AcRVGP2k expressing the G glycoprot...

journal_title：Applied biochemistry and biotechnology

authors： Batista FR,Greco KN,Astray RM,Jorge SA,Augusto EF,Pereira CA,Mendonça RZ,Moraes AM

更新日期：2011-01-01 00:00:00

abstract：:Seeking for simple, rapid, and environmental-friendly routes to produce metal nanoparticles is quite attractive for various biotechnological applications. Biological synthesis method of silver nanoparticles has been found very promising due to their non-toxicity and simplicity. Here, the spores of Bacillus stratospher...

journal_title：Applied biochemistry and biotechnology

authors： Hosseini-Abari A,Emtiazi G,Lee SH,Kim BG,Kim JH

更新日期：2014-09-01 00:00:00

abstract：:Iron and copper are essential nutrients for all living organisms as cofactors of many enzymes and play important roles in electron transport system (ETS) enzymes which have heme and iron-sulfur centers. In the present study, ETS enzymes, namely, succinate dehydrogenase (SDH) and cytochrome c oxidase (COX), activities ...

journal_title：Applied biochemistry and biotechnology

authors： Tavsan Z,Ayar Kayali H

更新日期：2013-08-01 00:00:00

abstract：:An integrated wet-milling and alkali pretreatment was applied to corn stover prior to enzymatic hydrolysis. The effects of NaOH concentration in the pretreatment on crystalline structure, chemical composition, and reducing-sugar yield of corn stover were investigated, and the mechanism of increasing reducing-sugar yie...

journal_title：Applied biochemistry and biotechnology

authors： He X,Miao Y,Jiang X,Xu Z,Ouyang P

更新日期：2010-04-01 00:00:00

abstract：:Bioethanol is considered a potential liquid fuel that can be produced from biomass by fermentation and distillation. Although most of the water is removed by distillation, the purity of ethanol is limited to 95-96 % due to the formation of a low-boiling point, water-ethanol azeotrope. To improve the use of ethanol as ...

journal_title：Applied biochemistry and biotechnology

authors： Tang B,Bi W,Row KH

更新日期：2013-02-01 00:00:00

abstract：:Agar-immobilized alkaliphilic Bacillus sp. AR-009 cells were used for xylanase production using batch and continuous culture. In a batch culture, maximum enzyme production was observed after 48 h and remained high up to 72 h. In repeated batch cultivation, immobilized cells produced an appreciable level of xylanase ac...

journal_title：Applied biochemistry and biotechnology

authors： Mamo G,Gessesse A

更新日期：2000-05-01 00:00:00

abstract：:The presence of high strength fats and oils in dairy industry wastewaters poses serious challenges for biological treatment systems, and, therefore, its pretreatment is necessary in order to remove them. In the present study, synthetic dairy wastewater prepared in the laboratory was pretreated using the sophorolipid-p...

journal_title：Applied biochemistry and biotechnology

authors： Daverey A,Pakshirajan K

更新日期：2011-03-01 00:00:00