Production of biodiesel by immobilized Candida sp. lipase at high water content.

abstract：:Alkaline phosphatase from hog intestine was immobilized to controlled-pore glass under various conditions. The specific activity of the enzyme was not diminished by immobilization. The influence of temperature and pH on the behavior and the stability of the immobilized enzyme preparations is discussed and compared to ...

journal_title：Applied biochemistry and biotechnology

authors： Pittner F

更新日期：1982-05-01 00:00:00

abstract：:The utility of rhamnolipids in industry is currently limited due to the high constraints in its economic production. In this scenario, the novel utility of sodium dodecyl sulphate (SDS) as carbon source could serve as promising cost-effective strategy. Screening of effective SDS biodegraders led to the isolation of Ps...

journal_title：Applied biochemistry and biotechnology

authors： Rebello S,Asok AK,Joseph SV,Joseph BV,Jose L,Mundayoor S,M S J

更新日期：2013-01-01 00:00:00

abstract：:The use of cheap, high-efficiency, and ecofriendly adsorbent has been studied as an alternative way for the removal of dyes from wastewater. This paper investigated the use of waste brewery's yeast for the removal of acid orange 7 from aqueous solution. The optimum removal of acid orange 7 was found to be 3.561 mg/g a...

journal_title：Applied biochemistry and biotechnology

authors： Wu Y,Hu Y,Xie Z,Feng S,Li B,Mi X

更新日期：2011-04-01 00:00:00

abstract：:Lupulones, naturally produced by glandular trichomes of hop (Humulus lupulus), are prenylated phloroglucinol derivatives that contribute the bitter flavor of beer and demonstrate antimicrobial and anticancer activities. It is appealing to develop microbial cell factories such that lupulones may be produced via ferment...

journal_title：Applied biochemistry and biotechnology

authors： Guo X,Shen H,Liu Y,Wang Q,Wang X,Peng C,Liu W,Zhao ZK

更新日期：2019-07-01 00:00:00

abstract：:An efficient metabolic control approach was developed to improve the industrial anaerobic fermentation of adenosylcobalamin (ado-cbl) by Propionibacterium freudenreichii. The effects of 5,6-dimethylbenzimidazole (DMB) on cell growth and ado-cbl biosynthesis were investigated. Subsequently, the results obtained from th...

journal_title：Applied biochemistry and biotechnology

authors： Wang P,Wang Y,Su Z

更新日期：2012-05-01 00:00:00

abstract：:A microbial cyanide sensor was prepared, consisting of immobilized Saccharomyces cerevisiae and an oxygen electrode. When the electrode was inserted into a solution containing glucose, the respiration activity of the microorganisms increased. The change in the respiration activity is monitored with the oxygen electrod...

journal_title：Applied biochemistry and biotechnology

authors： Nakanishi K,Ikebukuro K,Karube I

更新日期：1996-08-01 00:00:00

abstract：:A comparative analysis of the structural and functional aspects along with equilibrium unfolding of two homologous cutinases, Cut1 and Cut2, from Thermobifida fusca was carried out. The CD and fluorescence profile at different pH in the range of 6 to 9 showed no structural variations for both cutinases, indicating the...

journal_title：Applied biochemistry and biotechnology

authors： Hegde K,Dasu VV

更新日期：2014-09-01 00:00:00

abstract：:An integrated wet-milling and alkali pretreatment was applied to corn stover prior to enzymatic hydrolysis. The effects of NaOH concentration in the pretreatment on crystalline structure, chemical composition, and reducing-sugar yield of corn stover were investigated, and the mechanism of increasing reducing-sugar yie...

journal_title：Applied biochemistry and biotechnology

authors： He X,Miao Y,Jiang X,Xu Z,Ouyang P

更新日期：2010-04-01 00:00:00

abstract：:Gold nanomaterials (Au NMs) have attracted increasing attention in biomedicine due to their facile preparation, multifunctional modifications, unique optical and electrical properties, and good biocompatibility. The physicochemical properties of Au NMs at nanoscale, like size, shape, surface chemistry, and near field ...

journal_title：Applied biochemistry and biotechnology

authors： Jiang XM,Wang LM,Wang J,Chen CY

更新日期：2012-03-01 00:00:00

abstract：:The article describes preparation of new calixarene biopolymers consisting of the immobilization of convenience calixarene derivative onto cellulose and chitosan biopolymers, and the encapsulation of these calixarene biopolymers with Candida rugosa lipase within a chemical inert sol-gel supported by polycondensation w...

journal_title：Applied biochemistry and biotechnology

authors： Ozyilmaz E,Sayin S

更新日期：2013-08-01 00:00:00

abstract：:Detection of minute quantities of target antigens in serum samples (consisting of a mixture of proteins/biomolecules) can be achieved by enhancement of the capture efficiencies of heterogeneous immunosensors. An important process parameter which affects the capture of target analytes in such immunosensors is the pH of...

journal_title：Applied biochemistry and biotechnology

authors： Rath D,Kumar S,Panda S

更新日期：2019-04-01 00:00:00

abstract：:Biosurfactants are of considerable interest due to their biodegradability, low degree of toxicity, and diverse applications. However, the high production costs involved in the acquisition of biosurfactants underscore the need for optimization of the production process to enable viable application on an industrial scal...

journal_title：Applied biochemistry and biotechnology

authors： Campos JM,Stamford TL,Sarubbo LA

更新日期：2014-03-01 00:00:00

abstract：:Superoxide dismutase (SOD, EC 1.15.1.1) is an enzyme catalyzing the dismutation of superoxide radical to hydrogen peroxide and dioxygen. To date, four types of SODs--Cu/ZnSOD, MnSOD, FeSOD, and NiSOD--have been identified. In this study, SOD proteins of Brachypodium distachyon (L.) Beauv. were screened by utilization ...

journal_title：Applied biochemistry and biotechnology

authors： Filiz E,Koc I,Ozyigit II

更新日期：2014-07-01 00:00:00

abstract：:A new conjugate for the affinity chromatography of UDP-galactose:glycoprotein galactosyltransferase has been synthesized by coupling hen ovomucoid, a ligand similar to the acceptor substrate, to agarose.The hen ovomucoid-Sepharose conjugate binds galactosyl transferase more tightly that other acceptor-Sepharose conjug...

journal_title：Applied biochemistry and biotechnology

authors： Tadolini B,Hakim G

更新日期：1981-09-01 00:00:00

abstract：:Two types of (CNBr-activated) Agarose-staphylococcal endonuclease derivatives have been prepared, one with the enzyme uniformly distributed in the support, and the other with the enzyme preferentially bound in the most external part of the support particles; the latter were obtained using agarose of very small pores a...

journal_title：Applied biochemistry and biotechnology

authors： Guisan JM,Serrano J,Melo FV,Ballesteros A

更新日期：1987-02-01 00:00:00

abstract：:A comparative study was carried out to evaluate protease production in solid-state fermentation (SSF) and submerged fermentation (SmF) by nine different thermophilic fungi--Thermoascus aurantiacus Miehe, Thermomyces lanuginosus, T. lanuginosus TO.03, Aspergillus flavus 1.2, Aspergillus sp. 13.33, Aspergillus sp. 13.34...

journal_title：Applied biochemistry and biotechnology

authors： Macchione MM,Merheb CW,Gomes E,da Silva R

更新日期：2008-03-01 00:00:00

abstract：:Cross-linked enzyme aggregates (CLEAs) are a versatile and effective method for enzyme immobilization, which is exquisitely simple and amenable to rapid optimization. In this study, nature egg white, which is low cost, easily available, and nontoxic, was used as protein feeder to replace traditional protein feeder (bo...

journal_title：Applied biochemistry and biotechnology

authors： Jiang Y,Wang Q,He Y,Zhou L,Gao J

更新日期：2014-03-01 00:00:00

abstract：:We demonstrate the feasibility of algal lipid production and bioelectricity generation with concurrent treatment of municipal solid waste (MSW) leachate in a double-chamber algal-assisted microbial fuel cell (AAMFC). The cathode chamber was loaded with Synechococcus sp. and MSW leachate whereas anode chamber was loade...

journal_title：Applied biochemistry and biotechnology

authors： Lakshmidevi R,Gandhi NN,Muthukumar K

更新日期：2020-06-01 00:00:00

abstract：:Aliphatic chlorinated compounds, such as trichloroethylene (TCE) and tetrachloroethylene (PCE), are major contaminants of ground water. A single-pass packed-bed bioreactor was utilized to study the biodegradation of organic waste mixtures consisting of PCE, TCE, and other short-chain chlorinated organics. The bioreact...

journal_title：Applied biochemistry and biotechnology

authors： Lackey LW,Phelps TJ,Bienkowski PR,White DC

更新日期：1993-04-01 00:00:00

abstract：:Large-volume parenteral solutions were submitted to heat treatments after being inoculated with Bacillus stearothermophilus ATCC 7953 (Tr = 121 degrees C) and Bacillus subtilis ATCC 9372 (Tr = 104.5 degrees C) spores. The average decimal reduction time for B. stearothermophilus ranged from a D121 degrees C value of 1....

journal_title：Applied biochemistry and biotechnology

authors： Penna TC,Marques M,Machoshvili IA,Ishii M

更新日期：2002-04-01 00:00:00

abstract：:In this work, fibronectin purification from human plasma with the gelatin-immobilised poly(hydroxyethyl methacrylate) (PHEMA) cryogel has been evaluated. The PHEMA cryogel was prepared by cryo-polymerisation which proceeds in an aqueous solution of monomer frozen inside a plastic syringe. The PHEMA cryogel contained i...

journal_title：Applied biochemistry and biotechnology

authors： Perçin I,Aksöz E,Denizli A

更新日期：2013-09-01 00:00:00

abstract：:6-Tuliposides A (6-PosA) and B (6-PosB) are major secondary metabolites in tulip (Tulipa gesneriana), having an acyl group at the C-6 position of D-glucose. They serve as precursors of the antimicrobial α-methylene-γ-butyrolactones tulipalins A (PaA) and B (PaB). The conversions of 6-PosA/6-PosB to PaA/PaB are catalyz...

journal_title：Applied biochemistry and biotechnology

authors： Nomura T,Ogita S,Kato Y

更新日期：2019-05-01 00:00:00

abstract：:The coarse perlite 40-80 mesh was selected as an immobilizing material and put into a packed bed reactor (PBR) to continuously convert maltose to isomalto-oligosaccharides (IMOs). The PBR was prepared by mixing the thermo-inactivated cells (TIC) from Aspergillus niger J2 strain with the coarse perlite, then the mixtur...

journal_title：Applied biochemistry and biotechnology

authors： Huang Z,Li Z,Su Y,Zhu Y,Zeng W,Chen G,Liang Z

更新日期：2018-08-01 00:00:00

abstract：:We expressed a putative beta-galactosidase from Sulfolobus acidocaldarius in Escherichia coli and purified the recombinant enzyme using heat treatment and Hi-Trap ion-exchange chromatography. The resultant protein gave a single 57-kDa band by SDS-PAGE and had a specific activity of 58 U/mg. The native enzyme existed a...

journal_title：Applied biochemistry and biotechnology

authors： Park AR,Kim HJ,Lee JK,Oh DK

更新日期：2010-04-01 00:00:00

abstract：:The present study deals with the characterization of halotolerant protease produced by Bacillus aquimaris VITP4 strain isolated from Kumta coast, Karnataka, India. The studies were performed at 40 °C and pH 8 in Tris buffer. Metal ions such as Mn(2+) and Ca(2+) increased the proteolytic activity of the enzyme by 34 an...

journal_title：Applied biochemistry and biotechnology

authors： Thaz CJ,Jayaraman G

更新日期：2014-01-01 00:00:00

abstract：:Sweet sorghum (Sorghum sp.) has high biomass yield. Hydrolysis of lignocellulosic sweet sorghum bagasse (SSB) to fermentable sugar could be useful for manufacture of biofuel or other fermentation products. Pretreatment of lignocellulosic biomass to degrade lignin before enzymatic hydrolysis is a key step. Fungal pretr...

journal_title：Applied biochemistry and biotechnology

authors： Mishra V,Jana AK

更新日期：2017-09-01 00:00:00

abstract：:The purpose of this investigation was to study the effect of Streptomyces erumpens cells immobilized in various matrices, i.e., agar-agar, polyacrylamide, and luffa (Luffa cylindrica L.) sponge for production of alpha-amylase. Luffa sponge was found to be 21% and 51% more effective in enzyme yield than agar-agar and p...

journal_title：Applied biochemistry and biotechnology

authors： Kar S,Swain MR,Ray RC

更新日期：2009-02-01 00:00:00

abstract：:Endoinulinase production was achieved by heteroexpression of endoinulinase-encoding gene from Aspergillus ficuum which is an eukaryotic organism in Escherichia coli BL21 (DE3). Further analysis demonstrated that the native signal peptide existed in inu2 gene lowered the enzyme expression level. To realize extracellula...

journal_title：Applied biochemistry and biotechnology

authors： Wang P,Ma J,Zhang Y,Zhang M,Wu M,Dai Z,Jiang M

更新日期：2016-07-01 00:00:00

abstract：:Paraoxonase 1 (PON1: EC 3.1.8.1) is a calcium-dependent enzyme associated with high-density lipoproteins (HDLs) and has a protective effect against oxidation of low-density lipoproteins (LDLs) in mammals. PON1 is the best-studied member of a family of enzymes called serum paraoxonases, or PONs, identified in mammals a...

journal_title：Applied biochemistry and biotechnology

authors： Erzengin M,Demir D,Arslan M,Sinan S

更新日期：2014-08-01 00:00:00

abstract：:In rapidly growing developing countries, waste disposal is a major challenge. Current waste disposal methods (e.g., landfills and sewage treatment) incur costs and often are not employed; thus, wastes accumulate in the environment. To address this challenge, it is advantageous to create economic incentives to collect ...

journal_title：Applied biochemistry and biotechnology

authors： Lonkar S,Fu Z,Wales M,Holtzapple M

更新日期：2017-01-01 00:00:00