Abstract：
:Production costs of cellulosic biofuels can be lowered if cellulases are recovered and reused using particulate carriers that can be extracted after biomass hydrolysis. Such enzyme recovery was recently demonstrated using enzymogel nanoparticles with grafted polymer brushes loaded with cellulases. In this work, cellulase (NS50013) and β-glucosidase (Novozyme 188) were immobilized on enzymogels made of poly(acrylic acid) polymer brushes grafted to the surface of silica nanoparticles. Response surface methodology was used to model effects of pH and temperature on hydrolysis and recovery of free and attached enzymes. Hydrolysis yields using both enzymogels and free cellulase and β-glucosidase were highest at the maximum temperature tested, 50 °C. The optimal pH for cellulase enzymogels and free enzyme was 5.0 and 4.4, respectively, while both free β-glucosidase and enzymogels had an optimal pH near 4.4. Highest hydrolysis sugar concentrations with cellulase and β-glucosidase enzymogels were 69 and 53 % of those with free enzymes, respectively. Enzyme recovery using enzymogels decreased with increasing pH, but cellulase recovery remained greater than 88 % throughout the operating range of pH values less than 5.0 and was greater than 95 % at pH values below 4.3. Recovery of β-glucosidase enzymogels was not affected by temperature and had little impact on cellulase recovery.
journal_name
Appl Biochem Biotechnoljournal_title
Applied biochemistry and biotechnologyauthors
Samaratunga A,Kudina O,Nahar N,Zakharchenko A,Minko S,Voronov A,Pryor SWdoi
10.1007/s12010-015-1633-zsubject
Has Abstractpub_date
2015-06-01 00:00:00pages
1114-30issue
4eissn
0273-2289issn
1559-0291journal_volume
176pub_type
杂志文章abstract：:Aspergillus niger phytase (PhyA) has been used as a feed supplement to improve the bioavailability of phytate phosphorus to swine and poultry. However, it is unable to maintain its stability due to high temperature during the feed pelleting process. In this study, we performed site-directed mutagenesis in the Aspergil...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-013-0380-2
更新日期：2013-10-01 00:00:00
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journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-017-2439-y
更新日期：2017-09-01 00:00:00
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doi：10.1007/s12010-018-2903-3
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journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
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更新日期：2009-05-01 00:00:00
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journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-011-9367-z
更新日期：2011-12-01 00:00:00
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更新日期：2015-11-01 00:00:00
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journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
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更新日期：2011-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s12010-007-9214-4
更新日期：2007-04-01 00:00:00
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journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-007-9022-x
更新日期：2007-03-01 00:00:00
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更新日期：2012-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s12010-010-9022-0
更新日期：2011-01-01 00:00:00
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doi：10.1007/s12010-007-8034-x
更新日期：2008-03-01 00:00:00
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journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/BF02921683
更新日期：1991-08-01 00:00:00
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doi：10.1007/s12010-014-1252-0
更新日期：2015-01-01 00:00:00
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更新日期：2016-03-01 00:00:00
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更新日期：1997-04-01 00:00:00
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doi：10.1007/s12010-015-1621-3
更新日期：2015-06-01 00:00:00
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pub_type： 杂志文章
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更新日期：2020-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s12010-013-0482-x
更新日期：2014-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2014-02-01 00:00:00
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journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1385/abab:121:1-3:0085
更新日期：2005-04-01 00:00:00
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journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s12010-012-9755-z
更新日期：2012-08-01 00:00:00
abstract：:Electrokinetic-microbial remediation (EMR) has emerged as a promising option for the removal of polycyclic aromatic hydrocarbons (PAHs) from contaminated soils. The aim of this study was to enhance degradation of phenanthrene (Phe)-contaminated soils using EMR combined with biosurfactants. The electrokinetic (EK) reme...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s12010-015-1949-8
更新日期：2016-04-01 00:00:00
abstract：:Cross-linked enzyme aggregates (CLEAs) are a versatile and effective method for enzyme immobilization, which is exquisitely simple and amenable to rapid optimization. In this study, nature egg white, which is low cost, easily available, and nontoxic, was used as protein feeder to replace traditional protein feeder (bo...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-013-0697-x
更新日期：2014-03-01 00:00:00
