Investigation of Binding Affinity Between Prokaryotic Proteins (AHU-IHF) and DNAs: Steered Molecular Dynamics Approach.

abstract：:Superoxide dismutase (SOD, EC 1.15.1.1) is an enzyme catalyzing the dismutation of superoxide radical to hydrogen peroxide and dioxygen. To date, four types of SODs--Cu/ZnSOD, MnSOD, FeSOD, and NiSOD--have been identified. In this study, SOD proteins of Brachypodium distachyon (L.) Beauv. were screened by utilization ...

journal_title：Applied biochemistry and biotechnology

authors： Filiz E,Koc I,Ozyigit II

更新日期：2014-07-01 00:00:00

abstract：:In recent years, the concept of producing biodiesel from renewable lipid sources has regained international attention. In Brazil, a national program was launched in 2002 to evaluate the technical, economic, and environmental competitiveness of biodiesel in relation to the commercially available diesel oil. Several res...

journal_title：Applied biochemistry and biotechnology

authors： Ramos LP,Wilhelm HM

更新日期：2005-04-01 00:00:00

abstract：:Ovary-specific acidic protein (OSAP) is an important reproduction-related gene. In this study, we cloned the full-length cDNA sequence of porcine OSAP gene through the rapid amplification of cDNA ends method. The porcine OSAP gene encodes a protein of 238 amino acids which shares high homology with the OSAP of five sp...

journal_title：Applied biochemistry and biotechnology

authors： Lei Y,Yonggang L

更新日期：2012-09-01 00:00:00

abstract：:Batch, fed-batch, and semicontinuous fermentation processes were used for the production of xylitol from sugarcane bagasse hemicellulosic hydrolysate. The best results were achieved by the semicontinuous fermentation process: a xylitol yield of 0.79 g/g with an efficiency of 86% and a volumetric productivity of 0.66 g...

journal_title：Applied biochemistry and biotechnology

authors： Rodrigues DC,Silva SS,Prata AM,Felipe MG

更新日期：1998-04-01 00:00:00

abstract：:Recently, poly(3-hydroxybutyrate) (PHB) has been found in a few thermophilic strains where several advantages can be gained from running fermentation at high temperatures. Caldimonas manganoxidans, a thermophilic gram-negative bacterium, was investigated for the feasibility as a PHB-producing strain. It is suggested t...

journal_title：Applied biochemistry and biotechnology

authors： Hsiao LJ,Lin JH,Sankatumvong P,Wu TM,Li SY

更新日期：2016-11-01 00:00:00

abstract：:The effects of amino acid composition and peptide molecular mass on ACE-inhibitory and antioxidant activities of protein fragments obtained from tomato waste fermented using Bacillus subtilis were evaluated. The addition of B. subtilis increased the relative amounts of aromatic and positively-charged amino acids which...

journal_title：Applied biochemistry and biotechnology

authors： Moayedi A,Mora L,Aristoy MC,Hashemi M,Safari M,Toldrá F

更新日期：2017-01-01 00:00:00

abstract：:Trehalose synthase (TreS) from Meiothermus ruber was co-aggregated with polyethyleneimine (PEI) and precipitated with polyethylene glycol (PEG), followed by cross-linking with glutaraldehyde to obtain TreS-polyethyleneimine cross-linked enzyme aggregates (termed as CLEAs-PEI-PEG). The TreS solution at 0.5 mg mL(-1) pr...

journal_title：Applied biochemistry and biotechnology

authors： Zheng J,Chen Y,Yang L,Li M,Zhang J

更新日期：2014-11-01 00:00:00

abstract：:We expressed a putative beta-galactosidase from Sulfolobus acidocaldarius in Escherichia coli and purified the recombinant enzyme using heat treatment and Hi-Trap ion-exchange chromatography. The resultant protein gave a single 57-kDa band by SDS-PAGE and had a specific activity of 58 U/mg. The native enzyme existed a...

journal_title：Applied biochemistry and biotechnology

authors： Park AR,Kim HJ,Lee JK,Oh DK

更新日期：2010-04-01 00:00:00

abstract：:Methods for isothermal amplification of nucleic acids are gained more attention in the last two decades. For isothermal amplification, DNA polymerases with strand displacement activity are required, and Bst exo- is one of the most commonly used polymerases. However, Bst exo- is able to cause nonspecific DNA amplificat...

journal_title：Applied biochemistry and biotechnology

authors： Garafutdinov RR,Gilvanov AR,Sakhabutdinova AR

更新日期：2020-02-01 00:00:00

abstract：:A new conjugate for the affinity chromatography of UDP-galactose:glycoprotein galactosyltransferase has been synthesized by coupling hen ovomucoid, a ligand similar to the acceptor substrate, to agarose.The hen ovomucoid-Sepharose conjugate binds galactosyl transferase more tightly that other acceptor-Sepharose conjug...

journal_title：Applied biochemistry and biotechnology

authors： Tadolini B,Hakim G

更新日期：1981-09-01 00:00:00

abstract：:Lignocellulose biomass contain large macromolecules especially cellulose and hemicelluloses that can be converted to fuel and chemicals using microbial biocatalysts. This study presents comprehensive optimization of production of biomass-hydrolyzing enzymes (BHE) by a high β-glucosidase-producing Trichoderma SG2 for b...

journal_title：Applied biochemistry and biotechnology

authors： Nanjundaswamy A,Okeke BC

更新日期：2020-05-01 00:00:00

abstract：:Anthracnose is a major disease in Florida hybrid bunch grapes, caused by a fungus viz. Elsinoe ampelina. Florida hybrid bunch grapes are grown in southeastern USA for their superior wine characteristics. However, the effect of anthracnose on grape productivity and wine quality is a major concern to grape growers. Our ...

journal_title：Applied biochemistry and biotechnology

authors： Vasanthaiah HK,Katam R,Basha SM

更新日期：2009-06-01 00:00:00

abstract：:Ethanol from corn is produced using dry grind corn process in which simultaneous saccharification and fermentation (SSF) is one of the most critical unit operations. In this work an optimal controller based on a previously validated SSF model was developed by formulating the SSF process as a Bolza problem and using gr...

journal_title：Applied biochemistry and biotechnology

authors： Murthy GS,Johnston DB,Rausch KD,Tumbleson ME,Singh V

更新日期：2012-01-01 00:00:00

abstract：:The capacity for anaerobic decolorization of a sulfonated azo dye, Congo Red, by a strain of a sulfate-reducing bacterium was evaluated. After optimizing the growth rate of the bacteria on a simple carbon source and terminal electron acceptor pair, lactate and sulfate, respectively, the effect of the dye concentration...

journal_title：Applied biochemistry and biotechnology

authors： Diniz PE,Lopes AT,Lino AR,Serralheiro ML

更新日期：2002-03-01 00:00:00

abstract：:Bacterial strain Vibrio sp. (PIGB 184) isolated from water samples of the Arabian Sea and identified through 16S rRNA demonstrated the production of pigmentary antioxidants with higher ABTS activities 90.9 ± 0.42 % in comparison with the standard commercial pigmented antioxidant, quercetin 88.8 ± 1.4 %. Antioxidative ...

journal_title：Applied biochemistry and biotechnology

authors： Pawar R,Mohandass C,Dastager SG,Kolekar YM,Malwankar R

更新日期：2016-04-01 00:00:00

abstract：:Aspergillus fumigatus N2 was isolated from decaying wood. This strain produces extracellular xylanases and cellulases. The highest xylanase (91.9 U/mL) and CMCase (5.61 U/mL) activity was produced when 1% barley straw was used as the carbon source. The optimum pH and temperature for xylanase activity were 6.0 and 65 °...

journal_title：Applied biochemistry and biotechnology

authors： Lin C,Shen Z,Qin W

更新日期：2017-06-01 00:00:00

abstract：:The glpX gene (Rv1099c) of Mycobacterium tuberculosis (Mtb) encodes Fructose 1,6-bisphosphatase II (FBPase II; EC 3.1.3.11); a key gluconeogenic enzyme. Mtb possesses glpX homologue as the major known FBPase. This study explored the expression, purification and enzymatic characterization of functionally active FBPase ...

journal_title：Applied biochemistry and biotechnology

authors： Gutka HJ,Rukseree K,Wheeler PR,Franzblau SG,Movahedzadeh F

更新日期：2011-08-01 00:00:00

abstract：:Chemical modification was evaluated to stabilize pig kidney D-amino acid oxidase (pkDAAO), which is required for analytical determination of D-amino acids. Optimization of modification conditions was performed to obtain high recovery yield and stability, and chemical modification at 30 degrees C for 12 h with a highly...

journal_title：Applied biochemistry and biotechnology

authors： Bakke M,Kajiyama N

更新日期：2004-03-01 00:00:00

abstract：:Tyrosinase (T3824, Sigma) enzymes were immobilized onto SnO2: Sb thin films (ATO-TF) which were synthesized in laboratory conditions by spray pyrolysis technique. Immobilization of tyrosinase onto SnO2: Sb thin film (ATO-TF) was confirmed by scanning electron microscopy (SEM) and Fourier transformed infrared spectrosc...

journal_title：Applied biochemistry and biotechnology

authors： Türkhan A,Kaya ED,Koçyiğit A

更新日期：2020-10-01 00:00:00

abstract：:Dual-responsive poly(N,N-diethylacrylamide) (PDEA) hydrogel films with entrapped horseradish peroxidase (HRP) and glucose oxidase (GOD) were successfully prepared on electrode surface with a simple one-step polymerization procedure under mild conditions, designated as PDEA-HRP-GOD. Cyclic voltammetric (CV) response of...

journal_title：Applied biochemistry and biotechnology

authors： Yao H,Lin L,Wang P,Liu H

更新日期：2014-08-01 00:00:00

abstract：:This paper reports a study of the enzymatic esterification of oleic acid and ethanol. The reaction was catalyzed by lipases produced by solid-state fermentation with Rhizopus sp. Olive oil and perlite were used as an inducer and inert support, respectively. Synthesis of ethyl oleate was carried out in a 10-mL batch re...

journal_title：Applied biochemistry and biotechnology

authors： Martínez-Ruiz A,García HS,Saucedo-Castañeda G,Favela-Torres E

更新日期：2008-12-01 00:00:00

abstract：:Two DNA fragments containing the entire coding sequences of lactate dehydrogenase (LDH; ldhL1 and ldhD), whose enzymes have high activity for bioconversion of phenylpyruvate (PPA) to phenyllactate (PLA), were amplified from Lactobacillus plantarum SK002 using PCR. Sequencing showed open reading frames of 963 bp (ldhL1...

journal_title：Applied biochemistry and biotechnology

authors： Jia J,Mu W,Zhang T,Jiang B

更新日期：2010-09-01 00:00:00

abstract：:Genetic polymorphism of the cytochrome P450 (CYP) genes particularly affects CYP2D6 and CYP2C19 to a functionally relevant extent, and it is therefore crucial to elucidate the enzyme kinetic and molecular basis for altered catalytic activity of these allelic variants. This study explored the expression and function of...

journal_title：Applied biochemistry and biotechnology

authors： Dong AN,Pan Y,Palanisamy UD,Yiap BC,Ahemad N,Ong CE

更新日期：2018-09-01 00:00:00

abstract：:Performance of microbial carbon capture cells (MCCs), having a low-cost clayware separator, was evaluated in terms of wastewater treatment and electricity generation using algae Chlorella pyrenoidosa in MCC-1 and Anabaena ambigua in MCC-2 and without algae in a cathodic chamber of MCC-3. Higher power production was ac...

journal_title：Applied biochemistry and biotechnology

authors： Jadhav DA,Jain SC,Ghangrekar MM

更新日期：2017-11-01 00:00:00

abstract：:The present study was carried out to understand the effect of cortisol on caspase expression in the C2C12 and 3 T3-L1 cells under co-culture system. Cells were co-cultured by using transwell inserts with a 0.4-μm porous membrane to separate C2C12 and 3 T3-L1 preadipocyte cells. Each cell type was grown independently o...

journal_title：Applied biochemistry and biotechnology

authors： Muthuraman P

更新日期：2014-06-01 00:00:00

abstract：:Pectinases catalyze the degradation of pectic substances and are used in several processes, mainly in food and textile industries. In this study, a biomimetic matrix of alginate/gelatin/calcium oxalate (AGOCa) was synthesized for the in situ immobilization via encapsulation of crude pectinase from Aspergillus niger AT...

journal_title：Applied biochemistry and biotechnology

authors： Bustamante-Vargas CE,de Oliveira D,Valduga E,Venquiaruto LD,Paroul N,Backes GT,Dallago RM

更新日期：2016-07-01 00:00:00

abstract：:We demonstrate the feasibility of algal lipid production and bioelectricity generation with concurrent treatment of municipal solid waste (MSW) leachate in a double-chamber algal-assisted microbial fuel cell (AAMFC). The cathode chamber was loaded with Synechococcus sp. and MSW leachate whereas anode chamber was loade...

journal_title：Applied biochemistry and biotechnology

authors： Lakshmidevi R,Gandhi NN,Muthukumar K

更新日期：2020-06-01 00:00:00

abstract：:A lectin-like protein from the seeds of Acacia farnesiana was isolated from the albumin fraction, characterized, and sequenced by tandem mass spectrometry. The albumin fraction was extracted with 0.5 M NaCl, and the lectin-like protein of A. farnesiana (AFAL) was purified by ion-exchange chromatography (Mono-Q) follow...

journal_title：Applied biochemistry and biotechnology

authors： Santi-Gadelha T,Rocha BA,Oliveira CC,Aragão KS,Marinho ES,Gadelha CA,Toyama MH,Pinto VP,Nagano CS,Delatorre P,Martins JL,Galvani FR,Sampaio AH,Debray H,Cavada BS

更新日期：2008-07-01 00:00:00

abstract：:Biosorption is a potential tool for the extraction of metals from contaminated water and recovery of precious metals, which is a convenient alternative to conventional processes. In the present study, molybdenum recovery by Acidithiobacillus ferrooxidans strain ZT-94 was evaluated. Additionally, the effects of pH init...

journal_title：Applied biochemistry and biotechnology

authors： Kasra-Kermanshahi R,Tajer-Mohammad-Ghazvini P,Bahrami-Bavani M

更新日期：2020-11-17 00:00:00

abstract：:Based on the p426 series of expression vectors developed by Mumberg et al. (Gene 156, 119-122, 1995), we have generated a set of plasmids that allow the glucose-dependent expression of target genes in the yeast, Saccharomyces cerevisiae. The ADH1 promoter in plasmid p426-ADH1 was replaced by the 1-kb 5'-region from ei...

journal_title：Applied biochemistry and biotechnology

authors： Thierfelder S,Ostermann K,Göbel A,Rödel G

更新日期：2011-04-01 00:00:00