Purification and Characterization of a Novel β-Galactosidase From the Thermoacidophile Alicyclobacillus vulcanalis.

abstract：:Lignocellulose-decaying fungal bioplatforms available are not commercially accessible and are limited to short-term use. In this study, those limitations were overcome by developing a platform using water-soaked rice straw (RS) biodegraded by irradiation-based fungal pretreatment (IBFP). This eco-friendly system incre...

journal_title：Applied biochemistry and biotechnology

authors： Bak JS

更新日期：2015-12-01 00:00:00

abstract：:Kariba weed (Salvinia molesta) was used as biomass feedstock for ethanol production by separate hydrolysis and fermentation (SHF). Monosaccharides from Kariba weed hydrolysate were produced using thermal acid hydrolysis, sonication, and enzymatic saccharification. The optimal conditions for thermal acid hydrolysis of ...

journal_title：Applied biochemistry and biotechnology

authors： Kityo MK,Sunwoo I,Kim SH,Park YR,Jeong GT,Kim SK

更新日期：2020-09-01 00:00:00

abstract：:The objective of this work was to take advantage of the resting cells of suitable fungus as an in vitro model to prepare gastrodin from p-2-hydroxybenzyl alcohol (HBA), which mainly exists in the metabolites of the plant Gastrodia elata Blume. The one-step biotransformation of HBA into gastrodin was examined with the ...

journal_title：Applied biochemistry and biotechnology

authors： Fan L,Dong Y,Xu T,Zhang H,Chen Q

更新日期：2013-05-01 00:00:00

abstract：:This work describes the use of an ultrasound system for the enzymatic transesterification of oils using combi-lipases as biocatalyst. The reactions were carried out evaluating the individual use of waste oil and fresh soybean oil, and the immobilized lipases CALB, TLL, and RML were used as biocatalysts. It was perform...

journal_title：Applied biochemistry and biotechnology

authors： Poppe JK,Matte CR,Fernandez-Lafuente R,Rodrigues RC,Ayub MAZ

更新日期：2018-11-01 00:00:00

abstract：:With regard to the enzymatic synthesis of sorbitol and gluconic acid, a screening was carried out to identify promising producers of glucose-fructose oxidoreductase (GFOR) and gluconolactonase (GL). Zymomonas mobilis DSM 473 and Rhodotorula rubra DSM 70403 have been selected for the synthesis of GFOR and GL, respectiv...

journal_title：Applied biochemistry and biotechnology

authors： Nidetzky B,Fürlinger M,Gollhofer D,Haug I,Haltrich D,Kulbe KD

更新日期：1997-04-01 00:00:00

abstract：:The gate surface of an ion-sensitive field effect transistor (ISFET) was modified with Langmuir-Blodgett (LB) film composed of fatty acid or crown ether amphiphiles to examine their potentiometric response to H+ and K+ ions. The results demonstrate the possible use of the lipid films for preparing ISFET ion sensors. ...

journal_title：Applied biochemistry and biotechnology

authors： Osa T

更新日期：1993-04-01 00:00:00

abstract：:Efficient degradation of cellulose needs a synergistic reaction of the cellulolytic enzymes, which include exoglucanases, endoglucanases, and β-1,4-glucosidase. In this study, we used an improved Bac-to-Bac/BmNPV baculovirus expression system, which lacks the virus-encoded chitinase cathepsin (v-cath) genes of Bombyx ...

journal_title：Applied biochemistry and biotechnology

authors： Li XH,Wang MX,Zhang P,Hu JB,Sun CG,Liu XJ,Zhou F,Niu YS,Malik FA,Bhaskar R,Yang HJ,Miao YG

更新日期：2011-09-01 00:00:00

abstract：:This study evaluates the potential for using different effluents for simultaneous H(2) and CH(4) production in a two-stage batch fermentation process with mixed microflora. An appreciable amount of H(2) was produced from parboiled rice wastewater (23.9 mL g(-1) chemical oxygen demand [COD]) and vinasse (20.8 mL g(-1) ...

journal_title：Applied biochemistry and biotechnology

authors： Peixoto G,Pantoja-Filho JL,Agnelli JA,Barboza M,Zaiat M

更新日期：2012-10-01 00:00:00

abstract：:The most commonly used method for the measurement of the level of endo-xylanase in commercial enzyme preparations is the 3,5-dinitrosalicylic acid (DNS) reducing sugar method with birchwood xylan as substrate. It is well known that with the DNS method, much higher enzyme activity values are obtained than with the Nels...

journal_title：Applied biochemistry and biotechnology

authors： McCleary BV,McGeough P

更新日期：2015-11-01 00:00:00

abstract：:Carnosol and carnosic acid are polyphenolic compounds found in rosemary and sage with known anti-oxidant, anti-inflammatory, and anti-microbial properties. Here, we addressed the potential use of carnosol and carnosic acid for in vitro bone tissue engineering applications, specifically depending on their cytotoxic eff...

journal_title：Applied biochemistry and biotechnology

authors： Karadas O,Mese G,Ozcivici E

更新日期：2019-06-01 00:00:00

abstract：:Cofermentation of glucose, xylose, and arabinose is critical for complete bioconversion of lignocellulosic biomass, such as agricultural residues and herbaceous energy crops, to ethanol. We have previously developed a plasmid-bearing strain of Zymomonas mobilis (206C[pZB301]) capable of cofermenting glucose, xylose, a...

journal_title：Applied biochemistry and biotechnology

authors： Mohagheghi A,Evans K,Chou YC,Zhang M

更新日期：2002-04-01 00:00:00

abstract：:Molecular chaperones and protein folding factors of bacterial periplasmic space play important roles in assisting disulfide bond formation and proper protein folding. In this study, effects of disulfide bond protein (Dsb) families were investigated on assembly of 3F3 Fab, an antibody inhibitor targeting matrix metallo...

journal_title：Applied biochemistry and biotechnology

authors： Rodriguez C,Nam DH,Kruchowy E,Ge X

更新日期：2017-10-01 00:00:00

abstract：:Hexavalent chromium, Cr(6+), is a common and toxic pollutant in soils and waters. Reduction of the mobile Cr(6+) to the less mobile and less toxic trivalent chromium, Cr(3+), can be achieved with conventional chemical reduction technologies. Alternatively, Cr(6+) can be biochemically reduced to Cr(3+) by anaerobic mic...

journal_title：Applied biochemistry and biotechnology

authors： Turick CE,Camp CE,Apel WA

更新日期：1997-04-01 00:00:00

abstract：:The role of selenium (Se) supplementation on glutathione, a potent intracellular redox buffer, and trehalose, a well-known stress protectant molecule, was studied. The amount of glutathione decreased significantly while that of trehalose showed a minor decrease in the cells grown in Se-supplemented medium. After heat ...

journal_title：Applied biochemistry and biotechnology

authors： Sharma SC,Anand MS

更新日期：2006-04-01 00:00:00

abstract：:A microbial cyanide sensor was prepared, consisting of immobilized Saccharomyces cerevisiae and an oxygen electrode. When the electrode was inserted into a solution containing glucose, the respiration activity of the microorganisms increased. The change in the respiration activity is monitored with the oxygen electrod...

journal_title：Applied biochemistry and biotechnology

authors： Nakanishi K,Ikebukuro K,Karube I

更新日期：1996-08-01 00:00:00

abstract：:Genetic polymorphism of the cytochrome P450 (CYP) genes particularly affects CYP2D6 and CYP2C19 to a functionally relevant extent, and it is therefore crucial to elucidate the enzyme kinetic and molecular basis for altered catalytic activity of these allelic variants. This study explored the expression and function of...

journal_title：Applied biochemistry and biotechnology

authors： Dong AN,Pan Y,Palanisamy UD,Yiap BC,Ahemad N,Ong CE

更新日期：2018-09-01 00:00:00

abstract：:The enzyme cellobiase from Novo was immobilized in controlled pore silica particles by covalent binding with the silane-glutaraldehyde method with protein and activity yields of 67 and 13.7%, respectively. The activity of the free enzyme (FE) and immobilized enzyme (IE) was determined with 2 g/L of cellobiose, from 40...

journal_title：Applied biochemistry and biotechnology

authors： Calsavara LP,De Moraes FF,Zanin GM

更新日期：2001-04-01 00:00:00

abstract：:The primary structure of macrodontain I, a peptidase from Pseudananas macrodontes fruits, was determined using Edman's degradation. The enzyme is a non-glycosylated peptidase composed by 213 amino acids with a calculated molecular weight of 23,486.18 Da, pI value 6.99, and a molar extinction coefficient at 280 nm of 6...

journal_title：Applied biochemistry and biotechnology

authors： Errasti ME,Natalucci CL,Caffini NO,Rotelli AE,Brullo A,Maras B,Trejo SA,López LMI

更新日期：2018-09-01 00:00:00

abstract：:Lipases are an enzyme class of a great importance as biocatalysts applied to organic chemistry. However, it is still necessary to search for new enzymes with special characteristics such as good stability towards high temperatures, organic solvents, and high stereoselectivity presence. The present work's aim was to im...

journal_title：Applied biochemistry and biotechnology

authors： Cunha AG,Fernández-Lorente G,Gutarra ML,Bevilaqua JV,Almeida RV,Paiva LM,Fernández-Lafuente R,Guisán JM,Freire DM

更新日期：2009-05-01 00:00:00

abstract：:Biosurfactants are of considerable interest due to their biodegradability, low degree of toxicity, and diverse applications. However, the high production costs involved in the acquisition of biosurfactants underscore the need for optimization of the production process to enable viable application on an industrial scal...

journal_title：Applied biochemistry and biotechnology

authors： Campos JM,Stamford TL,Sarubbo LA

更新日期：2014-03-01 00:00:00

abstract：:A Bacillus subtilis strain isolated from contaminated soil from a refinery has been screened for biosurfactant production in crystal sugar (sucrose) with different nitrogen sources (NaNO(3), (NH(4))(2)SO(4), urea, and residual brewery yeast). The highest reduction in surface tension was achieved with a 48-h fermentati...

journal_title：Applied biochemistry and biotechnology

authors： Fonseca RR,Silva AJ,De França FP,Cardoso VL,Sérvulo EF

更新日期：2007-04-01 00:00:00

abstract：:Amyloglucosidase from Novo (Copenhagen, Denmark) was immobilized in controlled pore silica particles with the silane-glutaraldehyde covalent method. Thermal stability of the free and immobilized enzyme (IE) was determined with 30% (w/v) alpha-amylase liquefied cassava starch, pH 4.5, temperatures from 35 to 75 degrees...

journal_title：Applied biochemistry and biotechnology

authors： Zanin GM,De Moraes FF

更新日期：1998-04-01 00:00:00

abstract：:Numerous microcapsule systems have been developed for a wide range of applications, including the sustained release of drugs, cell transplantation for therapy, cell immobilization, and other biotechnological applications. Despite the fact that microcapsule membrane is a dominant factor governing overall microcapsule p...

journal_title：Applied biochemistry and biotechnology

authors： Chen H,Ouyang W,Lawuyi B,Lim T,Prakash S

更新日期：2006-09-01 00:00:00

abstract：:A biocompatible needle-type glucose sensor with a 3-electrode configuration was constructed. A platinum-electroplated carbon stick was used as the working electrode, Ag/AgCl as the reference electrode, and a disposable hypodermic needle made of stainless steel as the counter electrode. A Nafion membrane, an immobilize...

journal_title：Applied biochemistry and biotechnology

authors： Chen CY,Tamiya E,Ishihara K,Kosugi Y,Su YC,Nakabayashi N,Karube I

更新日期：1992-09-01 00:00:00

abstract：:Concentrated dilute acid hydrolysate was obtained from hot water extracts of Acer saccharum (sugar maple) and was fermented to ethanol by Pichia stipitis in a 1.3-L-benchtop bioreactor. The conditions under which the highest ethanol yield was achieved were when the air flow rate was set to 100 cm(3) and the agitation ...

journal_title：Applied biochemistry and biotechnology

authors： Shupe AM,Liu S

更新日期：2012-09-01 00:00:00

abstract：:The effect of surfactant on enzymatic digestibility was investigated during the pretreatment stage. Newspaper was pretreated with an ammonia-hydrogen peroxide mixture on a shaking bath at 40 degrees C and 130 strokes/min for 3 h. Two kinds of nonionic surfactants, NP series and Tween series, were utilized. The effect ...

journal_title：Applied biochemistry and biotechnology

authors： Kim SB,Chun JW

更新日期：2004-04-01 00:00:00

abstract：:Endophytes from medicinal plants represent a potential source of bioactive compounds. During the present investigation, fungal endophytes were isolated from turmeric (Curcuma longa), an important medicinal plant. A total of 207 endophytic fungal isolates were obtained from the rhizome of C. longa L. They were grouped ...

journal_title：Applied biochemistry and biotechnology

authors： Gupta S,Kaul S,Singh B,Vishwakarma RA,Dhar MK

更新日期：2016-11-01 00:00:00

abstract：:Tifton 85 bermudagrass, developed at the ARS-USDA in Tifton, GA, is grown on over ten million acres in the USA for hay and forage. Of the bermudagrass cultivars, Tifton 85 exhibits improved digestibility because the ratio of ether- to ester-linked phenolic acids has been lowered using traditional plant breeding techni...

journal_title：Applied biochemistry and biotechnology

authors： Ximenes EA,Brandon SK,Doran-Peterson J

更新日期：2008-03-01 00:00:00

abstract：:Forty-four endophytic fungal isolates obtained from marine sponge, Hyrtios erectus, were evaluated and screened for their hydrolase activities. Most of the isolates were found to be prolific producers of hydrolytic enzymes. Only 11 isolates exhibited maximum cellular contents of lipids, rhamnolipids, and protein in th...

journal_title：Applied biochemistry and biotechnology

authors： El-Gendy MMAA,Yahya SMM,Hamed AR,Soltan MM,El-Bondkly AMA

更新日期：2018-07-01 00:00:00

abstract：:Recombinant tissue-plasminogen activator (r-tPA), expressed in Escherichia coli cells in an aggregated form, was solubilized with a strong chaotrope in the absence of any reducing agent. The solubilized molecule was reactivated by a procedure that was developed to mimic the physiological conditions optimal for the fun...

journal_title：Applied biochemistry and biotechnology

authors： Grunfeld H,Patel A,Shatzman A,Nishikawa AH

更新日期：1992-05-01 00:00:00