Role of selenium supplementation and heat stress on trehalose and glutathione content in Saccharomyces cerevisiae.

abstract：:The aim of this work is to investigate the binding affinity between the prokaryotic proteins-AHU-IHF proteins (AHU (AHU2, TR3, and AHU6) and IHF (IHF-WT and IHF-βE44A))-and DNAs (DNA, H'-DNA, and H'44A-DNA) by using the steered molecular dynamics (SMD) simulation and the molecular mechanics Poisson-Boltzmann surface a...

journal_title：Applied biochemistry and biotechnology

authors： Nguyen H,Pham T,Nguyen HL,Phan T

更新日期：2018-12-01 00:00:00

abstract：:The glpX gene from Francisella tularensis encodes for the class II fructose 1,6-bisphosphatase (FBPaseII) enzyme. The glpX gene has been verified to be essential in F. tularensis, and the inactivation of this gene leads to impaired bacterial growth on gluconeogenic substrates. In the present work, we have complemented...

journal_title：Applied biochemistry and biotechnology

authors： Gutka HJ,Wolf NM,Bondoc JMG,Movahedzadeh F

更新日期：2017-12-01 00:00:00

abstract：:The mutant strain designated as ART18, obtained from the wild-type strain Clostridium acetobutylicum PW12 treated by atmospheric and room temperature plasma, showed higher solvent tolerance and butanol production than that of the wild-type strain. The production of butanol was 11.3 ± 0.5 g/L, 31 % higher than that of ...

journal_title：Applied biochemistry and biotechnology

authors： Li HG,Luo W,Wang Q,Yu XB

更新日期：2014-04-01 00:00:00

abstract：:Asphaltenes from a crude oil rich in heavy metals (Castilla crude oil) were fractionated and partially characterized. Biocatalytic modifications of these fractionated asphaltenes by three different hemoproteins: chloro-peroxidase (CPO), cytochrome C peroxidase (Cit-C), and lignin peroxidase (LPO) were evaluated in bot...

journal_title：Applied biochemistry and biotechnology

authors： Mogolloń L,Rodríguez R,Larrota W,Ortiz C,Torres R

更新日期：1998-04-01 00:00:00

abstract：:Possible methods to minimize the toxic effects of SOx and NOx on the growth of a highly CO2 tolerant and fast-growing microalga, Chlorella sp. KR-1, were investigated. Maintaining the pH of the culturing media at an adequate value was quite important to enhancing the tolerances of the microalgae to SOx and NOx. Contro...

journal_title：Applied biochemistry and biotechnology

authors： Lee JN,Lee JS,Shin CS,Park SC,Kim SW

更新日期：2000-04-01 00:00:00

abstract：:The increase in waste disposal and energy costs has provided an incentive to convert carbohydrate-rich food waste streams into fuel. For example, dining halls and restaurants discard foods that require tipping fees for removal. An effective use of food waste may be the enzymatic hydrolysis of the waste to simple sugar...

journal_title：Applied biochemistry and biotechnology

authors： Davis RA

更新日期：2008-03-01 00:00:00

abstract：:In this study, we investigated the dynamics of a computer simulation of a continuous alcoholic fermentation process combined with a flash column under vacuum. The alcohol was partially extracted in order to maintain its concentration at about 40 kg/m3 in the fermentor. The mathematical model of the fermentation was de...

journal_title：Applied biochemistry and biotechnology

authors： Costa AC,Dechechi EC,Silva FL,Maugeri F,Maciel R

更新日期：2000-04-01 00:00:00

abstract：:Dimethyl sulfide (DMS) is one of the sulfurous pollutants present in the waste gas generated from the pulp and paper industry. DMS has environmental health implications; therefore, it is necessary to treat the waste gas containing DMS prior to discharge into the environment. A bench-scale biofilter was operated in the...

journal_title：Applied biochemistry and biotechnology

authors： Giri BS,Juwarkar AA,Satpute DB,Mudliar SN,Pandey RA

更新日期：2012-07-01 00:00:00

abstract：:Crosslinked enzyme aggregates (CLEAs) of a partially purified penicillin acylase from a recombinant Escherichia coli strain have been produced as a novel type of biocatalysts well endowed to perform in organic media. Different protein precipitants were studied and glutaraldehyde was used as the crosslinking agent. Pre...

journal_title：Applied biochemistry and biotechnology

authors： Illanes A,Wilson L,Caballero E,Fernández-Lafuente R,Guisán JM

更新日期：2006-06-01 00:00:00

abstract：:Adenosine monophosphate-activated protein kinase (AMPK) is viewed as a privileged therapeutic target for several diseases such as cancer, diabetes, inflammation, obesity, etc. In addition, AMPK has entered the limelight of current drug discovery with its evolution as a key metabolic regulator. AMPK also plays a key ro...

journal_title：Applied biochemistry and biotechnology

authors： Ramesh M,Vepuri SB,Oosthuizen F,Soliman ME

更新日期：2016-02-01 00:00:00

abstract：:The high demand of human placenta-derived mesenchymal stem cells (hPDMSCs) for therapeutic applications requires reproducible production of large numbers of well-characterized cells under well-controlled conditions. However, no method for fast hPDMSCs proliferation has yet been reported. In the present study, the feas...

journal_title：Applied biochemistry and biotechnology

authors： Yu Y,Li K,Bao C,Liu T,Jin Y,Ren H,Yun W

更新日期：2009-10-01 00:00:00

abstract：:Bioreduction of 1-phenyl-2-propanone to prepare (S)-1-phenyl-2-propanol, a useful pharmaceutical intermediate, was performed with growing cells of Rhodococcus erythropolis JX-021, giving 14 mM (1.9 g/L) product in 99% e.e. at 5 h in the catalysis of 15 mM substrate. The reduction stopped afterwards due to strong inhib...

journal_title：Applied biochemistry and biotechnology

authors： Jin JZ,Li H,Zhang J

更新日期：2010-11-01 00:00:00

abstract：:The objective of the present work was to evaluate the metabolic effects induced by the initial glucose concentration (IGC) on the cultivation of Streptococcus zooepidemicus for the production of hyaluronic acid (HA). These effects were monitored along non-controlled pH cultivations, carried out in 250-mL Erlenmeyer fl...

journal_title：Applied biochemistry and biotechnology

authors： Pires AM,Santana MH

更新日期：2010-11-01 00:00:00

abstract：:L-asparaginase production was optimized using isolated Bacillus circulans (MTCC 8574) under solid-state fermentation (SSF) using locally available agricultural waste materials. Among different agricultural materials (red gram husk, bengal gram husk, coconut, and groundnut cake), red gram husk gave the maximum enzyme p...

journal_title：Applied biochemistry and biotechnology

authors： Hymavathi M,Sathish T,Subba Rao Ch,Prakasham RS

更新日期：2009-10-01 00:00:00

abstract：:Fluorescence spectroscopy, UV-visible absorption spectroscopy, circular dichroism (CD) spectroscopy, viscometry, cyclic voltammetry (CV), and differential pulse voltammetry (DPV) were applied to investigate the competitive interaction of DNA with two aromatic α-aminobisphosphonates and neutral red dye (NR, intercalato...

journal_title：Applied biochemistry and biotechnology

authors： Gholivand MB,Peyman H,Gholivand K,Roshanfekr H,Taherpour AA,Yaghobi R

更新日期：2017-07-01 00:00:00

abstract：:In recent years, the concept of producing biodiesel from renewable lipid sources has regained international attention. In Brazil, a national program was launched in 2002 to evaluate the technical, economic, and environmental competitiveness of biodiesel in relation to the commercially available diesel oil. Several res...

journal_title：Applied biochemistry and biotechnology

authors： Ramos LP,Wilhelm HM

更新日期：2005-04-01 00:00:00

abstract：:Changes in protein glycosylation owing to changes in environmental conditions are not well understood. To better understand these relationships, methods to quantify controlling factors are needed. Because enzymes are translated from genes, the ability to quantify gene expression levels for glycosylation-related enzyme...

journal_title：Applied biochemistry and biotechnology

authors： Clark KJ,Harcum SW,Griffiths J,Bailey KM

更新日期：2005-06-01 00:00:00

abstract：:Reed plants (Phragmites communis (Linn.) Trin) are hydrophilic perennial grasses growing in fresh and brackish waters. These plants readily adapt to arid and high salinity conditions; however, their resistance mechanism against abiotic stresses, especially high salinity, is largely unknown. In the present study, we cl...

journal_title：Applied biochemistry and biotechnology

authors： Zhang X,Quan G,Wang J,Han H,Chen S,Guo S,Yin H

更新日期：2015-04-01 00:00:00

abstract：:A novel mannan-specific lectin was isolated from the roots of a traditional Chinese herbal medicine, Ophioglossum pedunculosum through ion-exchange chromatography and gel filtration. With a molecular mass of 19,835.7 Da demonstrated by MALDI-TOF analysis, this novel agglutinin was designated as O. pedunculosum aggluti...

journal_title：Applied biochemistry and biotechnology

authors： He XM,Ji N,Xiang XC,Luo P,Bao JK

更新日期：2011-12-01 00:00:00

abstract：:This work is focused on the biotransformation of R-(+)-limonene and (-)-beta-pinene to bioflavor production. To carry out the present study, 405 microorganisms were tested for their ability to bioconvert the substrates. From the isolated microorganisms, 193 were selected in the prescreening using mineral medium for li...

journal_title：Applied biochemistry and biotechnology

authors： Rottava I,Cortina PF,Grando CE,Colla AR,Martello E,Cansian RL,Toniazzo G,Treichel H,Antunes OA,Oestreicher EG,de Oliveira D

更新日期：2010-10-01 00:00:00

abstract：:A spectrophotometric method of measuring oxygenase activity in cell extracts or in zymograms was developed. It is an easy and cheap method that allows spectrophotometric measurement of activity by a colored reaction and reveals activity bands in a polyacrylamide gel electrophoresis (PAGE) gel as brown bands. To prove ...

journal_title：Applied biochemistry and biotechnology

authors： Zazueta-Sandoval R,Novoa VZ,Jiménez HS,Ortiz RC

更新日期：2003-04-01 00:00:00

abstract：:Analysis of peroxidase activity by native polyacrylamide gel electrophoresis (PAGE) from a garlic bulb (Allium sativum L) extract showed two major activities (designated POX1 and POX2). The POX2 isoenzyme was purified to homogeneity by ammonium sulfate precipitation, gel filtration, and cation-exchange chromatography....

journal_title：Applied biochemistry and biotechnology

authors： Marzouki SM,Limam F,Smaali MI,Ulber R,Marzouki MN

更新日期：2005-12-01 00:00:00

abstract：:A new conjugate for the affinity chromatography of UDP-galactose:glycoprotein galactosyltransferase has been synthesized by coupling hen ovomucoid, a ligand similar to the acceptor substrate, to agarose.The hen ovomucoid-Sepharose conjugate binds galactosyl transferase more tightly that other acceptor-Sepharose conjug...

journal_title：Applied biochemistry and biotechnology

authors： Tadolini B,Hakim G

更新日期：1981-09-01 00:00:00

abstract：:A new bioprocess for production of sorbitol and gluconic acid from two low-cost feedstocks, inulin and cassava starch, using a commercially available enzyme was proposed in this study. The commercial glucoamylase GA-L NEW from Genencor was found to demonstrate a high inulinase activity for hydrolysis of inulin into fr...

journal_title：Applied biochemistry and biotechnology

authors： An K,Hu F,Bao J

更新日期：2013-12-01 00:00:00

abstract：:This research describes the development of a multi-analyte lateral-flow immunoassay intended for the simultaneous detection of three anti-protozoan drugs (coccidiostats). These drugs, namely, halofuginone, toltrazuril and diclazuril, are used in the treatment of Eimeria spp. infections in cattle, pigs, chickens and tu...

journal_title：Applied biochemistry and biotechnology

authors： Fitzgerald J,Leonard P,Danaher M,O'Kennedy R

更新日期：2015-05-01 00:00:00

abstract：:The authors have recently reported on the design of a protein (MB-1) enriched in methionine, threonine, lysine, and leucine. The protein is intended to be produced by rumen bacteria, in a way that would provide high producing lactating cows with limiting amino acids. In this report, MB-1 stability in the rumen is asse...

journal_title：Applied biochemistry and biotechnology

authors： MacCallum JD,Hefford MA,Omar S,Beauregard M

更新日期：1997-04-01 00:00:00

abstract：:The glpX gene (Rv1099c) of Mycobacterium tuberculosis (Mtb) encodes Fructose 1,6-bisphosphatase II (FBPase II; EC 3.1.3.11); a key gluconeogenic enzyme. Mtb possesses glpX homologue as the major known FBPase. This study explored the expression, purification and enzymatic characterization of functionally active FBPase ...

journal_title：Applied biochemistry and biotechnology

authors： Gutka HJ,Rukseree K,Wheeler PR,Franzblau SG,Movahedzadeh F

更新日期：2011-08-01 00:00:00

abstract：:Convenient expression systems for efficient heterologous production of different laccases are needed for their characterization and application. The laccase cDNAs lcc1 and lcc2 from Trametes versicolor were expressed in Pichia pastoris and Aspergillus niger under control of their respective glyceraldehyde-3-phosphate ...

journal_title：Applied biochemistry and biotechnology

authors： Bohlin C,Jönsson LJ,Roth R,van Zyl WH

更新日期：2006-04-01 00:00:00

abstract：:Thermoacidophiles are microorganisms capable of optimum growth under a combination of high temperature and low pH. These microorganisms are a rich source of thermo- and acid- active/stable glycosyl hydrolases. Such enzymes could find use as novel biocatalysts in industrial processes, as operation at elevated temperatu...

journal_title：Applied biochemistry and biotechnology

authors： Murphy J,Ryan MP,Walsh G

更新日期：2020-07-01 00:00:00

abstract：:This work aimed at setting up a fully instrumented, laboratory-scale bioreactor enabling anaerobic valorization of solid substrates through hydrogen and/or volatile fatty acid (VFA) production using mixed microbial populations (consortia). The substrate used was made of meat-based wastes, especially from slaughterhous...

journal_title：Applied biochemistry and biotechnology

authors： Pessiot J,Nouaille R,Jobard M,Singhania RR,Bournilhas A,Christophe G,Fontanille P,Peyret P,Fonty G,Larroche C

更新日期：2012-07-01 00:00:00