Isolation and characterization of dimethyl sulfide (DMS)-degrading bacteria from soil and biofilter treating waste gas containing DMS from the laboratory and pulp and paper industry.

Abstract:

:Dimethyl sulfide (DMS) is one of the sulfurous pollutants present in the waste gas generated from the pulp and paper industry. DMS has environmental health implications; therefore, it is necessary to treat the waste gas containing DMS prior to discharge into the environment. A bench-scale biofilter was operated in the laboratory as well as in a pulp and paper industry for the treatment of DMS. Both the biofilters were packed with pre-sterilized wood chips and cow dung/compost of the same origin seeded with biomass developed from garden soil enriched with DMS. The biofilters were operated for the generation of process parameters, and the potential microorganisms isolated from both the biofilters have been purified and characterized for degradation of DMS. Further, these cultures were purified on a basal medium using DMS as a sole carbon source for the growth. Further, the purified cultures were characterized through standard fatty acid methyl esters (FAME)-gas chromatography method, and the isolates were found to be mesophilic, aerobic microbes. These microbes were identified as Bacillus sphaericus-GC subgroup F, Paenibacillus polymyxa, B. sphaericus-GC subgroup F, B. sphaericus-GC subgroup F, and Bacillus megaterium-GC subgroup A, respectively. The potential culture for degradation of DMS was identified as B. sphaericus by 16s rRNA molecular analysis.

authors

Giri BS,Juwarkar AA,Satpute DB,Mudliar SN,Pandey RA

doi

10.1007/s12010-012-9647-2

subject

Has Abstract

pub_date

2012-07-01 00:00:00

pages

1744-52

issue

6

eissn

0273-2289

issn

1559-0291

journal_volume

167

pub_type

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