Abstract:
:A new conjugate for the affinity chromatography of UDP-galactose:glycoprotein galactosyltransferase has been synthesized by coupling hen ovomucoid, a ligand similar to the acceptor substrate, to agarose.The hen ovomucoid-Sepharose conjugate binds galactosyl transferase more tightly that other acceptor-Sepharose conjugates.The new adsorbent gives comparable yields and purifications with those obtained by ligands similar to the nucleotide moiety of the substrate and to the "specifier" protein, α-lactalbumin.The soluble galactosyltransferase from rat ventral prostate is effectively removed from the high speed supernatant by an ovomucoid-Sepharose column. The enzyme can be eluted with buffer containing EDTA andN-acetylglucosamine in a high yield (75-80%) and in a purified form (4000-fold purification). The stability of ovomucoid to heat and to high concentrations of urea and its inhibition of some proteases makes the conjugate easy to operate with an quite useful even with rather crude preparations.
journal_name
Appl Biochem Biotechnoljournal_title
Applied biochemistry and biotechnologyauthors
Tadolini B,Hakim Gdoi
10.1007/BF02780797subject
Has Abstractpub_date
1981-09-01 00:00:00pages
193-9issue
3eissn
0273-2289issn
1559-0291journal_volume
6pub_type
杂志文章abstract::The presence of high strength fats and oils in dairy industry wastewaters poses serious challenges for biological treatment systems, and, therefore, its pretreatment is necessary in order to remove them. In the present study, synthetic dairy wastewater prepared in the laboratory was pretreated using the sophorolipid-p...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
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更新日期:2011-03-01 00:00:00
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更新日期:2009-08-01 00:00:00
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journal_title:Applied biochemistry and biotechnology
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pub_type: 杂志文章,评审
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journal_title:Applied biochemistry and biotechnology
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doi:10.1007/s12010-013-0709-x
更新日期:2014-03-01 00:00:00
abstract::In the fermentation of lignocellulose hydrolysates to ethanol, two major problems are encountered: the fermentation of the pentose sugar xylose, and the presence of microbial inhibitors. Xylose can be directly fermented with yeasts, such as Pachysolen tannophilus, Candida shehatae, and Pichia stipis, or by isomerizati...
journal_title:Applied biochemistry and biotechnology
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doi:10.1007/BF02922595
更新日期:1991-04-01 00:00:00
abstract::Tradescantia pallida (Wandering jew)-a succulent perennial herb-was screened to be a potent chromium (Cr) accumulator. Its ability to grow under Cr stress was examined by studying biochemical changes and physiological response of the plant in presence of 5-20 mg L(-1) Cr(VI) concentration in hydroponic environment for...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-014-1035-7
更新日期:2014-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s12010-008-8207-2
更新日期:2008-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1385/abab:107:1-3:493
更新日期:2003-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s12010-009-8803-9
更新日期:2010-10-01 00:00:00
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更新日期:2019-05-01 00:00:00
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journal_title:Applied biochemistry and biotechnology
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更新日期:2012-07-01 00:00:00
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journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
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更新日期:2014-07-01 00:00:00
abstract::NADH : FMN oxidoreductase and bacterial luciferase have been efficiently coimmobilized onto Sepharose 4B. This luminescent immobilized enzyme system can be used to assay NADH. The assay is rapid and sensitive with a lower limit of detection of 0.2 pmol/assay tube. The intra-assay precision was 3.5% at 2 × 10(-5) M and...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/BF02799178
更新日期:1982-11-01 00:00:00
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journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章,评审
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更新日期:2012-03-01 00:00:00
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journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1385/abab:117:1:01
更新日期:2004-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2013-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s12010-011-9380-2
更新日期:2011-12-01 00:00:00
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journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-012-9770-0
更新日期:2012-09-01 00:00:00
abstract::Marine Aspergillus awamori BTMFW032, recently reported by us, produce acidophilic tannase as extracellular enzyme. Here, we report the application of this enzyme for synthesis of propyl gallate by direct transesterification of tannic acid and in tea cream solubilisation besides the simultaneous production of gallic ac...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-011-9162-x
更新日期:2011-07-01 00:00:00