Isolation and characterization of an atypical LEA protein coding cDNA and its promoter from drought-tolerant plant Prosopis juliflora.

abstract：:The increased additive amount of adjuncts in the raw materials of Chinese beer requires the usage of protease to release more water-soluble proteins. Here, a metallo-neutral protease suited for brewing industry was purified from Bacillus amyloliquefaciens SYB-001. A 5.6-fold purification of the neutral protease was ac...

journal_title：Applied biochemistry and biotechnology

authors： Wang J,Xu A,Wan Y,Li Q

更新日期：2013-08-01 00:00:00

abstract：:The gene encoding a thermostable β-D-xylosidase (GbtXyl43B) from Geobacillus thermoleovorans IT-08 was cloned in pET30a and expressed in Escherichia coli; additionally, characterization and kinetic analysis of GbtXyl43B were carried out. The gene product was purified to apparent homogeneity showing M r of 72 by sodium...

journal_title：Applied biochemistry and biotechnology

authors： Ratnadewi AA,Fanani M,Kurniasih SD,Sakka M,Wasito EB,Sakka K,Nurachman Z,Puspaningsih NN

更新日期：2013-08-01 00:00:00

abstract：:The article describes preparation of new calixarene biopolymers consisting of the immobilization of convenience calixarene derivative onto cellulose and chitosan biopolymers, and the encapsulation of these calixarene biopolymers with Candida rugosa lipase within a chemical inert sol-gel supported by polycondensation w...

journal_title：Applied biochemistry and biotechnology

authors： Ozyilmaz E,Sayin S

更新日期：2013-08-01 00:00:00

abstract：:This study shows that electroanalysis may be used in vanillin biotechnological production. As a matter of fact, vanillin and some molecules implicated in the process like eugenol, ferulic acid, and vanillic acid may be oxidized on electrodes made of different materials (gold, platinum, glassy carbon). By a judicious c...

journal_title：Applied biochemistry and biotechnology

authors： Giraud W,Mirabel M,Comtat M

更新日期：2014-02-01 00:00:00

abstract：:Artemisinin is an important drug commonly used in the treatment of malaria as a combination therapy. It is primarily produced by a plant Artemisia annua, however, its supply from plant is significantly lower than its huge demand and therefore alternative in vitro production routes are sought. Hairy root cultivation co...

journal_title：Applied biochemistry and biotechnology

authors： Patra N,Srivastava AK

更新日期：2014-11-01 00:00:00

abstract：:In this work, the catalytic conversion of natural polymer chitosan to 5-HMF under low temperature hydrothermal condition was investigated. The highest 5-HMF yield of 12.1 wt% was obtained under the following conditions: 174 °C reaction temperature, 2.2 % catalyst amount, and 36.9 min reaction time. In the effect of co...

journal_title：Applied biochemistry and biotechnology

authors： Lee SB,Jeong GT

更新日期：2015-06-01 00:00:00

abstract：:With regard to the enzymatic synthesis of sorbitol and gluconic acid, a screening was carried out to identify promising producers of glucose-fructose oxidoreductase (GFOR) and gluconolactonase (GL). Zymomonas mobilis DSM 473 and Rhodotorula rubra DSM 70403 have been selected for the synthesis of GFOR and GL, respectiv...

journal_title：Applied biochemistry and biotechnology

authors： Nidetzky B,Fürlinger M,Gollhofer D,Haug I,Haltrich D,Kulbe KD

更新日期：1997-04-01 00:00:00

abstract：:The corn wet milling process produces a 10% (w/w of the processed corn) byproduct called corn fiber, which is utilized worldwide as a low-value feedstock for cattle. The aim of this study was to find a higher value use of corn fiber. The main fractions of corn fiber are: 20% starch, 40% hemicellulose, 14% cellulose, a...

journal_title：Applied biochemistry and biotechnology

authors： Kálmán G,Recseg K,Gáspár M,Réczey K

更新日期：2006-03-01 00:00:00

abstract：:Four endoglucanase temperature isoforms (T (30), T (50), T (70), and T (90)) were identified and purified from the cladodes of the xerophytic plant Opuntia vulgaris. These isoforms exhibited optimum catalytic activity at 30 °C, 50 °C, 70 °C, and 90 °C and yielded an apparent molecular mass of 150, 20, 74, and 45 kDa, ...

journal_title：Applied biochemistry and biotechnology

authors： Shyamala S,Ravikumar S,Vikramathithan J,Srikumar K

更新日期：2011-12-01 00:00:00

abstract：:The saccharification of marine microalgae using amylase from marine bacteria in saline conditions was investigated. An amylase-producing bacterium, Pseudoalterimonas undina NKMB 0074 was isolated and identified. The green microalga NKG 120701 was determined to have the highest concentration of intracellular carbohydra...

journal_title：Applied biochemistry and biotechnology

authors： Matsumoto M,Yokouchi H,Suzuki N,Ohata H,Matsunaga T

更新日期：2003-04-01 00:00:00

abstract：:Prevalence of heavy metals in the living environment causes chemical stress and reactive oxygen species (ROS) formation in Phanerochaete chrysosporium (P. chrysosporium). However, the mechanisms involved in ROS defense are still under investigation. In the present study, we evaluated the effect of lead- and cadmium-in...

journal_title：Applied biochemistry and biotechnology

authors： Zhang Q,Zeng G,Chen G,Yan M,Chen A,Du J,Huang J,Yi B,Zhou Y,He X,He Y

更新日期：2015-02-01 00:00:00

abstract：:Nostoc microscopicum an effective extracellular polymers (EPs) synthesizer among cyanobacteria was isolated, and its elementary morphological features were defined with the aid of light microscope and CLSM. Bioseparation of EPs from 44 days-aged culture gave 0.90 g/L of the dry powdered extract. Chemical quantificatio...

journal_title：Applied biochemistry and biotechnology

authors： Ramachandran L,Marappa N,Sethumadhavan K,Nooruddin T

更新日期：2020-05-01 00:00:00

abstract：:The purpose of this study was to investigate the feasibility of immobilizing Yarrowia lipolytica lipase lip2 on epoxy microspheres with or without gelatin modifications. The activity of lipase immobilized on gelatin-modified supports was twofold higher than those immobilized on native supports. There was no significan...

journal_title：Applied biochemistry and biotechnology

authors： Xie R,Cui C,Chen B,Tan T

更新日期：2015-10-01 00:00:00

abstract：:In this study, the gene encoding an α-amylase from a psychrophilic Arthrobacter agilis PAMC 27388 strain was cloned into a pET-28a(+) vector and heterologously expressed in Escherichia coli BL21(DE3). The recombinant α-amylase with a molecular mass of about 80 kDa was purified by using Ni2+-NTA affinity chromatography...

journal_title：Applied biochemistry and biotechnology

authors： Kim SM,Park H,Choi JI

更新日期：2017-03-01 00:00:00

abstract：:Mouse CXCL14/BRAK is a monocyte-selective chemokine which is expressed in almost all normal tissues. A flood of reports on its new functions of tumor suppression and fat metabolism modulation has left CXCL14 a potential therapeutic candidate for these diseases. Therefore, a simple accessible method is on demand for la...

journal_title：Applied biochemistry and biotechnology

authors： Li J,Gao J,Sah S,Satyal U,Zhang R,Han W,Yu Y

更新日期：2011-08-01 00:00:00

abstract：:Based on the p426 series of expression vectors developed by Mumberg et al. (Gene 156, 119-122, 1995), we have generated a set of plasmids that allow the glucose-dependent expression of target genes in the yeast, Saccharomyces cerevisiae. The ADH1 promoter in plasmid p426-ADH1 was replaced by the 1-kb 5'-region from ei...

journal_title：Applied biochemistry and biotechnology

authors： Thierfelder S,Ostermann K,Göbel A,Rödel G

更新日期：2011-04-01 00:00:00

abstract：:For producing a large quantity of human nerve growth factor beta (hNGF-beta), the recombinant adenovirus containing an expression cassette of hNGF-beta complementary DNA gene was constructed and then instilled directly into goats' mammary glands. The recombinant hNGF-beta was detected in the milk. The maximal expressi...

journal_title：Applied biochemistry and biotechnology

authors： Xiao B,Li Q,Feng B,Han Z,Gao D,Zhao R,Li J,Li K,Zhi X,Yang H,Liu Z

更新日期：2009-06-01 00:00:00

abstract：:The two six-coordinate Pt(IV) complexes, containing bidentate nitrogen donor/methyl ligands with general formula [Pt(X)2Me2((t)bu2bpy)], where (t)bu2bpy = 4,4'-ditert-butyl-2,2'-bipyridine and X = Cl (C1) or Br (C2), serving as the leaving groups were synthesized for evaluation of their anticancer activities and DNA b...

journal_title：Applied biochemistry and biotechnology

authors： Pouryasin Z,Yousefi R,Nabavizadeh SM,Rashidi M,Hamidizadeh P,Alavianmehr MM,Moosavi-Movahedi AA

更新日期：2014-03-01 00:00:00

abstract：:Penicillin V acylase (PVA) is a pharmaceutically important enzyme as it plays a vital role in the manufacture of semi-synthetic beta-lactam antibiotics. Rhodotorula aurantiaca (NCIM 3425) produced high levels of intracellular penicillin V acylase after 18 h at pH 8.0 and temperature 27 degrees C. Fructose was the best...

journal_title：Applied biochemistry and biotechnology

authors： Kumar A,Singh S,Poddar P,Prabhune A,Pundle A

更新日期：2009-06-01 00:00:00

abstract：:A study was carried out to select the conditions for cultivation of Kluyveromyces marxianus CDBBL 278 in solid-state culture (SSC) using polyurethane foam (PUF) as an inert support. PUF was impregnated with culture media containing lactose (50 g/L) as the carbon and energy source. Evaluation of culture parameters duri...

journal_title：Applied biochemistry and biotechnology

authors： Tovar-Castro L,García-Garibay M,Saucedo-Castañeda G

更新日期：2008-12-01 00:00:00

abstract：:Analysis of peroxidase activity by native polyacrylamide gel electrophoresis (PAGE) from a garlic bulb (Allium sativum L) extract showed two major activities (designated POX1 and POX2). The POX2 isoenzyme was purified to homogeneity by ammonium sulfate precipitation, gel filtration, and cation-exchange chromatography....

journal_title：Applied biochemistry and biotechnology

authors： Marzouki SM,Limam F,Smaali MI,Ulber R,Marzouki MN

更新日期：2005-12-01 00:00:00

abstract：:Pretreatment based on aqueous ammonia was investigated under two different modes of operation: soaking in aqueous ammonia and ammonia recycle percolation. These processes were applied to three different feedstocks with varied composition: corn stover, high lignin (HL), and low lignin (LL) hybrid poplars. One of the im...

journal_title：Applied biochemistry and biotechnology

authors： Gupta R,Kim TH,Lee YY

更新日期：2008-03-01 00:00:00

abstract：:Methanol, acetone and diethyl ether extracts of Alpinia galanga have been evaluated against pathogens viz. Bacillus subtilis MTCC 2391, Enterobacter aerogene, Enterobacter cloacae, Enterococcus faecalis, Escherichia coli MTCC 1563, Klebsiella pneumoniae, Pseudomonas aeruginosa MTCC 6642, Salmonella typhimurium, Staphy...

journal_title：Applied biochemistry and biotechnology

authors： Rao K,Ch B,Narasu LM,Giri A

更新日期：2010-10-01 00:00:00

abstract：:A unicellular marine green alga, Chlamydomonas perigranulata, was demonstrated to synthesize starch through photosynthesis, store it in a cell, and ferment it under anaerobic conditions in the dark to produce ethanol, 2,3-butanediol (butanediol), acetic acid, and carbon dioxide (CO2). Previous fermentation data of an ...

journal_title：Applied biochemistry and biotechnology

authors： Hon-Nami K

更新日期：2006-04-01 00:00:00

abstract：:The common view of amyloids and prion proteins is that they are associated with many currently incurable diseases and present a great danger to an organism. This danger comes from the fact that not only prion proteins, but also the infectious form(s) of amyloids, as it has been shown recently, are able to transmit the...

journal_title：Applied biochemistry and biotechnology

authors： Pulawski W,Ghoshdastider U,Andrisano V,Filipek S

更新日期：2012-04-01 00:00:00

abstract：:Cross-linking of enzyme aggregates from recombinant Geotrichum sp. lipase based on polyethylenimine (PEI) was applied to hydrolyze fish oil for enrichment of polyunsaturated fatty acids successfully. Through acetone precipitation and cross-linking of physical aggregates using glutaraldehyde in the presence of PEI, fir...

journal_title：Applied biochemistry and biotechnology

authors： Yan J,Gui X,Wang G,Yan Y

更新日期：2012-02-01 00:00:00

abstract：:Application of statistical experimental designs for optimization of fermentation parameters to enhance ethanol production, which is an economical and renewable energy source using Saccharomyces cerevisiae NCIM 3090 from palmyra jaggery, was studied in a batch fermentor. Using Plackett-Burman design, impeller speed, co...

journal_title：Applied biochemistry and biotechnology

authors： Bandaru VV,Bandaru SR,Somalanka SR,Mendu DR,Imandi SB,Bejawada SR,Medicherla NR,Devarajan T,Karothi J,Chityala A

更新日期：2007-12-01 00:00:00

abstract：:Extracts from different higher plants were screened for the ability to inhibit the growth of Penicillium roqueforti, a major contaminating species in industrial food processing. Oat (Avena sativa) seed extracts exhibited a high degree of antifungal activity and could be used directly on rye bread to prevent the format...

journal_title：Applied biochemistry and biotechnology

authors： Sørensen HP,Madsen LS,Petersen J,Andersen JT,Hansen AM,Beck HC

更新日期：2010-03-01 00:00:00

abstract：:A two-phase partitioning bioreactor to treat gas effluents polluted by volatile organic compound has been developed. In this work, both the mass transfer of isopropylbenzene (IPB) and oxygen have been considered in relation to their influence on the hydrodynamics of the reactor and the type of silicone oils used as a ...

journal_title：Applied biochemistry and biotechnology

authors： Aldric JM,Lecomte JP,Thonart P

更新日期：2009-05-01 00:00:00

abstract：:Fluorescent and incandescent lighting systems were applied for batch photofermentative hydrogen production by four purple non-sulfur photosynthetic bacteria (PNSB). The hydrogen production efficiency of Rhodopseudomonas palustris, Rhodobacter sphaeroides, Rhodobacter capsulatus, and Rhodospirillum rubrum was evaluated...

journal_title：Applied biochemistry and biotechnology

authors： Hu C,Choy SY,Giannis A

更新日期：2018-05-01 00:00:00