Abstract:
:A simple, sensitive aptamer-based biosensor for the detection of phenylalanine is developed using the electrochemical transduction method. For this proposed aptasensor, a 5-thiol-terminated aptamer is covalently attached onto a gold electrode. At the first time, the electrode was evaluated as an electrochemical aptasensor for determination of phenylalanine in aqueous solutions. This sensor was tested in a Tris-HCl buffer with physiological pH = 7.4 by cyclic voltammetry and differential pulse voltammetry. The detection limit and sensitivity of the modified electrode toward phenylalanine were estimated to be 1 nM (S/N = 3) and 0.367 μA nM(-1), respectively. The linear range of the signal was observed between 1 and 10 nM of phenylalanine with 0.9914 correlation factor. The herein-described approach is expected to promote the exploitation of aptamer-based biosensors for protein assays in biochemical and biomedical studies.
journal_name
Appl Biochem Biotechnoljournal_title
Applied biochemistry and biotechnologyauthors
Omidinia E,Shadjou N,Hasanzadeh Mdoi
10.1007/s12010-013-0656-6subject
Has Abstractpub_date
2014-02-01 00:00:00pages
2070-80issue
4eissn
0273-2289issn
1559-0291journal_volume
172pub_type
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