Modification of lysine residues of horseradish peroxidase and its effect on stability and structure of the enzyme.

abstract：:Several recombinant derivatives of serine protease inhibitor called silk protease inhibitor 2 (SPI2), which is a silk component in Galleria mellonella (Lepidoptera, Insecta), were prepared in the expression vector Pichia pastoris. Both the native and the recombinant protease inhibitors were highly active against subti...

journal_title：Applied biochemistry and biotechnology

authors： Kodrík D,Kludkiewicz B,Navrátil O,Skoková Habuštová O,Horáčková V,Svobodová Z,Vinokurov KS,Sehnal F

更新日期：2013-09-01 00:00:00

abstract：:Aroma volatiles in Basmati-370, Ambemohar-157 (non-basmati scented), and IR-64 (non-scented) rice cultivars were qualitatively and quantitatively analyzed at vegetative and maturity stages to study their differential accumulation using headspace solid-phase microextraction, followed by gas chromatography mass spectrom...

journal_title：Applied biochemistry and biotechnology

authors： Hinge V,Patil H,Nadaf A

更新日期：2016-02-01 00:00:00

abstract：:A new process for enzymatic synthesis of biodiesel at high water content (10-20%) with 96% conversion by lipase from Candida sp. 99-125 was studied. The lipase, a no-position-specific lipase, was immobilized by a cheap cotton membrane and the membrane-immobilized lipase could be used at least six times with high conve...

journal_title：Applied biochemistry and biotechnology

authors： Tan T,Nie K,Wang F

更新日期：2006-02-01 00:00:00

abstract：:To prevent illegal use of clenbuterol and for quality control in the food industry, more efficient and reliable methods for clenbuterol detection are needed. In this study, clenbuterol was detected using a spectral imaging surface plasmon resonance sensor system via two inhibition methods: (1) the target site compensa...

journal_title：Applied biochemistry and biotechnology

authors： Wu Y,Yao M,Fang X,Yang Y,Cheng X

更新日期：2015-11-01 00:00:00

abstract：:A new cellulase producer strain of Penicillium digitatum (RV 06) was previously obtained from rotten maize grains. This work aim was to optimize the production and characterize this microorganism produced cellulase. A CMCase maximum production (1.6 U/mL) was obtained in stationary liquid culture, with an initial pH of...

journal_title：Applied biochemistry and biotechnology

authors： Dos Santos FC,de Oliveira MAS,Seixas FAV,Barbosa-Tessmann IP

更新日期：2020-09-01 00:00:00

abstract：:Binding of phenyl derivative of pyranocoumarin (PDP) modulated activity of fungal endopolygalacturonase in silico. Induced fit docking study of PDP with endopolygalacturonase (1HG8) showed a bifurcated hydrogen bond interaction with the protein at Lys 244 with a docking score of -3.6 and glide energy of -37.30 kcal/mo...

journal_title：Applied biochemistry and biotechnology

authors： Sangeetha S,Sarada DV

更新日期：2015-01-01 00:00:00

abstract：:We used ultrafiltration (UF) to evaluate membrane filtration characteristics of thin stillage and determine solids and nutrient compositions of filtered streams. To obtain thin stillage, corn was fermented using laboratory methods. UF experiments were conducted in batch mode under constant temperature and flow rate co...

journal_title：Applied biochemistry and biotechnology

authors： Arora A,Dien BS,Belyea RL,Wang P,Singh V,Tumbleson ME,Rausch KD

更新日期：2011-05-01 00:00:00

abstract：:The performance of immobilized Bifidobacterium longum in sodium alginate beads and on a spiral-sheet bioreactor for the production of lactic acid from cheese whey was evaluated. Lactose utilization and lactic acid yield of B. longum were compared with those of Lactobacillus helveticus. B. longum immobilized in sodium ...

journal_title：Applied biochemistry and biotechnology

authors： Shahbazi A,Mims MR,Li Y,Shirley V,Ibrahim SA,Morris A

更新日期：2005-04-01 00:00:00

abstract：:The corn wet milling process produces a 10% (w/w of the processed corn) byproduct called corn fiber, which is utilized worldwide as a low-value feedstock for cattle. The aim of this study was to find a higher value use of corn fiber. The main fractions of corn fiber are: 20% starch, 40% hemicellulose, 14% cellulose, a...

journal_title：Applied biochemistry and biotechnology

authors： Kálmán G,Recseg K,Gáspár M,Réczey K

更新日期：2006-03-01 00:00:00

abstract：:The microheterogeneous native amylolytic complex secreted by the isolate A6 of Lactobacillus plantarum revealed a selective enzyme specificity loss when submitted to a limited proteolysis under a suboptimum pH condition. A clear electrophoretic profile change toward just one shorter, more acidic, and equally active po...

journal_title：Applied biochemistry and biotechnology

authors： Florêncio JA,Eiras-Stofella DR,Soccol CR,Raimbault M,Guyot JP,Fontana JD

更新日期：2000-04-01 00:00:00

abstract：:The stabilization of olive recombinant hydroperoxide lyases (rHPLs) was investigated using selected chemical additives. Two rHPLs were studied: HPL full-length and HPL with its chloroplast transit peptide deleted (matured HPL). Both olive rHPLs are relatively stable at 4 °C, and enzyme activity can be preserved (about...

journal_title：Applied biochemistry and biotechnology

authors： Jacopini S,Vincenti S,Mariani M,Brunini-Bronzini de Caraffa V,Gambotti C,Desjobert JM,Muselli A,Costa J,Tomi F,Berti L,Maury J

更新日期：2017-07-01 00:00:00

abstract：:The present study involved strategies for enhancement in in vitro azadirachtin (commercially used biopesticide) production by hairy root cultivation of Azadirachta indica. Improvement in the azadirachtin production via triggering its biosynthetic pathway in plant cells was carried out by the exogenous addition of prec...

journal_title：Applied biochemistry and biotechnology

authors： Srivastava S,Srivastava AK

更新日期：2014-02-01 00:00:00

abstract：:Nephelium lappaceum L., commonly known as "rambutan," is a typical tropical tree and is well known for its juicy and sweet fruit which has an exotic flavor. Chemical studies on rambutan have led to the identification of various components such as monoterpene lactones and volatile compounds. Here, a 22.5-kDa trypsin in...

journal_title：Applied biochemistry and biotechnology

authors： Fang EF,Ng TB

更新日期：2015-04-01 00:00:00

abstract：:The production of a thermophilic 2-deoxyribose-5-phosphate aldolases (DERA) in Escherichia coli BL21 under continuous lactose induction strategy was investigated. The process was combined with the exponential feeding method, controlling the feeding rate to maintain the specific growth rate at 0.15 h(-1). The results i...

journal_title：Applied biochemistry and biotechnology

authors： Pei XL,Wang QY,Li CL,Qiu XF,Xie KL,Huang LF,Wang AM,Zeng ZW,Xie T

更新日期：2011-09-01 00:00:00

abstract：:Agricultural waste being the major solid waste in the environment, the study has explored and identified the presence of minerals especially silica in the agricultural waste like sugarcane bagasse ash and rice husk ash by carbonization on combustion at different thermal conditions and biodegradation. Presence of silic...

journal_title：Applied biochemistry and biotechnology

authors： Krishnamoorthy S,Iyer NR,Murthy AR

更新日期：2015-02-01 00:00:00

abstract：:Graphene-containing 13-93 bioactive glass and poly(ε-caprolactone)-based bilayer, electrically conductive scaffolds were prepared for osteochondral tissue repair. Biological response of osteoblastic MC3T3-E1 and chondrogenic ATDC5 cells to the composite scaffolds was assessed under mono-culture and co-culture conditio...

journal_title：Applied biochemistry and biotechnology

authors： Deliormanlı AM,Atmaca H

更新日期：2018-12-01 00:00:00

abstract：:The tunable textural properties of self-oriented mesoporous silica were investigated for their suitability as enzyme immobilization matrices to support transesterification of rice bran oil. Different morphologies of mesoporous silica (rod-like, rice-like, and spherical) were synthesized and characterized by scanning e...

journal_title：Applied biochemistry and biotechnology

authors： Ramachandran P,Narayanan GK,Gandhi S,Sethuraman S,Krishnan UM

更新日期：2015-03-01 00:00:00

abstract：:The enzyme mannose isomerase (EC 5.3.1.7) catalyzes the isomerization of D-mannose and D-fructose. The conversion of mannose to fructose is the first step in the principal pathway of mannose dissimilation by Pseudomonas cepacia. This enzyme is induced during growth on medium containing mannose to levels three- to four...

journal_title：Applied biochemistry and biotechnology

authors： Allenza P,Morrell MJ,Detroy RW

更新日期：1990-04-01 00:00:00

abstract：:Amyloglucosidase from Novo (Copenhagen, Denmark) was immobilized in controlled pore silica particles with the silane-glutaraldehyde covalent method. Thermal stability of the free and immobilized enzyme (IE) was determined with 30% (w/v) alpha-amylase liquefied cassava starch, pH 4.5, temperatures from 35 to 75 degrees...

journal_title：Applied biochemistry and biotechnology

authors： Zanin GM,De Moraes FF

更新日期：1998-04-01 00:00:00

abstract：:Light-harvesting chlorophyll a/b-binding proteins (LHCB) have been implicated in the stress response. In this study, a gene encoding LHCB in the pigeon pea was cloned and characterized. Based on the sequence of a previously obtained 327 bp Est, a full-length 793 bp cDNA was cloned using the rapid amplification of cDNA...

journal_title：Applied biochemistry and biotechnology

authors： Qiao G,Wen XP,Zhang T

更新日期：2015-12-01 00:00:00

abstract：:The host-pathogen interaction and involvement of calcium (Ca2+) signaling in tuberculosis infection is crucial and plays a significant role in pathogenesis. Ca2+ is known as a ubiquitous second messenger that could control multiple processes and is included in cellular activities like division, motility, stress respon...

journal_title：Applied biochemistry and biotechnology

authors： Sharma S,Meena LS

更新日期：2017-02-01 00:00:00

abstract：:Pretreatment based on aqueous ammonia was investigated under two different modes of operation: soaking in aqueous ammonia and ammonia recycle percolation. These processes were applied to three different feedstocks with varied composition: corn stover, high lignin (HL), and low lignin (LL) hybrid poplars. One of the im...

journal_title：Applied biochemistry and biotechnology

authors： Gupta R,Kim TH,Lee YY

更新日期：2008-03-01 00:00:00

abstract：:Ovarian malignancy is diagnosed in nearly a fourth of a million women internationally every year. Methylation of RASSF1A tumor suppressor gene prompts its inactivation in diseases. In this study, the RASSF1A promoter methylation was detected by methylated-specific PCR and investigated serum RASSF1A protein level throu...

journal_title：Applied biochemistry and biotechnology

authors： Rezk NA,Mohamed RH,Alnemr AA,Harira M

更新日期：2018-05-01 00:00:00

abstract：:Thermoacidophiles are microorganisms capable of optimum growth under a combination of high temperature and low pH. These microorganisms are a rich source of thermo- and acid- active/stable glycosyl hydrolases. Such enzymes could find use as novel biocatalysts in industrial processes, as operation at elevated temperatu...

journal_title：Applied biochemistry and biotechnology

authors： Murphy J,Ryan MP,Walsh G

更新日期：2020-07-01 00:00:00

abstract：:The effect of thidiazuron (TDZ) has been investigated in shoot multiplication for a simple, efficient, rapid, and commercially applicable regeneration protocol of an important medicinal plant, Vitex trifolia. Multiple shoots were induced in nodal explants obtained from a mature tree on Murashige and Skoog (MS) medium ...

journal_title：Applied biochemistry and biotechnology

authors： Ahmed MR,Anis M

更新日期：2012-11-01 00:00:00

abstract：:Steviol glycosides (SVglys) and gibberellins are originated from the shared biosynthesis pathway in Stevia (Stevia rebaudiana Bertoni). In this research, two experiments were conducted to study the opposing effects of external gibberellin (GA3) and Daminozide (a gibberellin inhibitor) on Stevia growth and metabolites....

journal_title：Applied biochemistry and biotechnology

authors： Karimi M,Hashemi J,Ahmadi A,Abbasi A,Pompeiano A,Tavarini S,Guglielminetti L,Angelini LG

更新日期：2015-01-01 00:00:00

abstract：:Operational practice of high-rate anaerobic bioreactors such as upflow anaerobic sludge bed (UASB) reactors is generally based on maximization of the biomass concentration and, in the case of more than one reactor compartment, operation in parallel. In this article, a modeling approach is used to postulate that the tr...

journal_title：Applied biochemistry and biotechnology

authors： Kleerebezem R

更新日期：2003-04-01 00:00:00

abstract：:We engineered Rhizopus chinensis lipase to study its critical amino acid role in catalytic properties. Based on the amino acid sequence and three-dimensional model of the lipase, residues located in its lid hinge region (Met93 and Thr96) were replaced with corresponding amino acid residues (Ile93 and Asn96) found in t...

journal_title：Applied biochemistry and biotechnology

authors： Zhu SS,Li M,Yu X,Xu Y

更新日期：2013-05-01 00:00:00

abstract：:Bacillus amyloliquefaciens K103 isolated from a lemon sample was used as a biocontrol agent to suppress Rhizoctonia solani Kühn and other fungal plant pathogens. Two antifungal compounds were purified from the culture broth using acid precipitation, gel permeation chromatography, and reversed-phase high-performance li...

journal_title：Applied biochemistry and biotechnology

authors： Zhang B,Dong C,Shang Q,Cong Y,Kong W,Li P

更新日期：2013-12-01 00:00:00

abstract：:The rheological behavior of a sulfated galactan extracted from Halymenia durvillei, a red seaweed collected in the coastal waters of a small island of Madagascar (Nosy-be in Indian Ocean), was investigated in dilute and semi-dilute solutions. In dilute solution with NaCl at 0.3 M, the polysaccharide adopted a coil con...

journal_title：Applied biochemistry and biotechnology

authors： Fenoradosoa TA,Laroche C,Delattre C,Dulong V,Le Cerf D,Picton L,Michaud P

更新日期：2012-07-01 00:00:00