Preparation of cross-linked enzyme aggregates of trehalose synthase via co-aggregation with polyethyleneimine.

abstract：:Large-volume parenteral solutions were submitted to heat treatments after being inoculated with Bacillus stearothermophilus ATCC 7953 (Tr = 121 degrees C) and Bacillus subtilis ATCC 9372 (Tr = 104.5 degrees C) spores. The average decimal reduction time for B. stearothermophilus ranged from a D121 degrees C value of 1....

journal_title：Applied biochemistry and biotechnology

authors： Penna TC,Marques M,Machoshvili IA,Ishii M

更新日期：2002-04-01 00:00:00

abstract：:Milk is a natural nutraceutical produced by mammals. The nanovesicles of milk play a role in horizontal gene transfer and confer health-benefits to milk consumers. These nanovesicles contain miRNA, mRNA, and proteins which mediate the intercellular communication. In this work, we isolated and characterized the buffalo...

journal_title：Applied biochemistry and biotechnology

authors： Baddela VS,Nayan V,Rani P,Onteru SK,Singh D

更新日期：2016-02-01 00:00:00

abstract：:Agar-immobilized alkaliphilic Bacillus sp. AR-009 cells were used for xylanase production using batch and continuous culture. In a batch culture, maximum enzyme production was observed after 48 h and remained high up to 72 h. In repeated batch cultivation, immobilized cells produced an appreciable level of xylanase ac...

journal_title：Applied biochemistry and biotechnology

authors： Mamo G,Gessesse A

更新日期：2000-05-01 00:00:00

abstract：:This study investigated methane production in an anaerobic sequencing batch biofilm reactor (AnSBBR) by co-digesting sugarcane vinasse and cheese whey. The assessment was based on the influence of feed strategy, interaction between cycle time and influent concentration, applied volumetric organic load (OLRA), and temp...

journal_title：Applied biochemistry and biotechnology

authors： Sousa SP,Lovato G,Albanez R,Ratusznei SM,Rodrigues JAD

更新日期：2019-11-01 00:00:00

abstract：:The nuclear receptor Rev-erbβ is a potent transcriptional factor whose functional study has been limited by the lack of suitable antibodies against it. To better understand Rev-erbβ's biological roles, we generated five hybridoma cell lines secreting antibodies against human Rev-erbβ in mice immunized with the purifie...

journal_title：Applied biochemistry and biotechnology

authors： Chen F,Li Y,Zhao J,Mao Q,Xia H

更新日期：2017-07-01 00:00:00

abstract：:The triosephosphate isomerase of Leishmania donovani (LdTIM) was expressed at high level in Escherichia coli. The TIM gene was cloned in expression vector pET-23(a) with C-terminal 6× His tag fused in frame, and expressed as a 27.6-kDa protein in E. coli as inclusion bodies. The recombinant LdTIM from E. coli lysate w...

journal_title：Applied biochemistry and biotechnology

authors： Kumar K,Bhargava P,Roy U

更新日期：2011-08-01 00:00:00

abstract：:Anaerobic digestion (AD) process is a well-established method to generate energy from the organic wastes both from the environmental and economical perspectives. The purpose of present study is to evaluate energy production from potato wastes by incorporating cow manure into the process. Firstly, a laboratory pilot of...

journal_title：Applied biochemistry and biotechnology

authors： Sanaei-Moghadam A,Abbaspour-Fard MH,Aghel H,Aghkhani MH,Abedini-Torghabeh J

更新日期：2014-08-01 00:00:00

abstract：:The aim of this work is to investigate the binding affinity between the prokaryotic proteins-AHU-IHF proteins (AHU (AHU2, TR3, and AHU6) and IHF (IHF-WT and IHF-βE44A))-and DNAs (DNA, H'-DNA, and H'44A-DNA) by using the steered molecular dynamics (SMD) simulation and the molecular mechanics Poisson-Boltzmann surface a...

journal_title：Applied biochemistry and biotechnology

authors： Nguyen H,Pham T,Nguyen HL,Phan T

更新日期：2018-12-01 00:00:00

abstract：:Cross-linking of enzyme aggregates from recombinant Geotrichum sp. lipase based on polyethylenimine (PEI) was applied to hydrolyze fish oil for enrichment of polyunsaturated fatty acids successfully. Through acetone precipitation and cross-linking of physical aggregates using glutaraldehyde in the presence of PEI, fir...

journal_title：Applied biochemistry and biotechnology

authors： Yan J,Gui X,Wang G,Yan Y

更新日期：2012-02-01 00:00:00

abstract：:The recombinant Escherichia coli gamma-glutamyltranspeptidase (EcGGT) was immobilized in Ca-alginate-kappa-carrageenan beads. Effects of alginate concentration, amount of loading enzyme, and bead size on the entrapped activity were investigated. Optimum alginate concentration for EcGGT immobilization was found to be 2...

journal_title：Applied biochemistry and biotechnology

authors： Hung CP,Lo HF,Hsu WH,Chen SC,Lin LL

更新日期：2008-08-01 00:00:00

abstract：:Protein-A or goat anti-mouse-Ig (GAMIg) covalently bound to agarose-polyacrolein microsphere beads (APAMB) were employed for the removal of T cells from human peripheral blood leukocytes (PBL) and bone marrow (BM). The cell suspensions were treated with a monoclonal anti-T cell antibody (Leu-1) or monoclonal antilymph...

journal_title：Applied biochemistry and biotechnology

authors： Weiss L,Margel S,Slavin S

更新日期：1986-10-01 00:00:00

abstract：:The effect of methanol on trichloroethylene (TCE) degradation by mixed and pure methylotrophic cultures was examined in batch culture experiments. Methanol was found to relieve growth inhibition of Methylosinus trichosporium (OB3b) at high (14 mg/L) TCE concentrations. Degradation of TCE was determined by both radiola...

journal_title：Applied biochemistry and biotechnology

authors： Eng W,Palumbo AV,Sriharan S,Strandberg GW

更新日期：1991-04-01 00:00:00

abstract：:Unlike silkworm (Bombyx mori) fibroin (SF), weaver ant (Oecophylla smaragdina) fibroin (WAF) is much less studied. Due to differences in amino acid composition and protein structure, this work aimed to produce the recombinant WAF protein, designated as WAF1, and investigated on its potential application as a biomateri...

journal_title：Applied biochemistry and biotechnology

authors： Khamhaengpol A,Siri S

更新日期：2017-09-01 00:00:00

abstract：:The common view of amyloids and prion proteins is that they are associated with many currently incurable diseases and present a great danger to an organism. This danger comes from the fact that not only prion proteins, but also the infectious form(s) of amyloids, as it has been shown recently, are able to transmit the...

journal_title：Applied biochemistry and biotechnology

authors： Pulawski W,Ghoshdastider U,Andrisano V,Filipek S

更新日期：2012-04-01 00:00:00

abstract：:L-asparaginase production was optimized using isolated Bacillus circulans (MTCC 8574) under solid-state fermentation (SSF) using locally available agricultural waste materials. Among different agricultural materials (red gram husk, bengal gram husk, coconut, and groundnut cake), red gram husk gave the maximum enzyme p...

journal_title：Applied biochemistry and biotechnology

authors： Hymavathi M,Sathish T,Subba Rao Ch,Prakasham RS

更新日期：2009-10-01 00:00:00

abstract：:Culture fluorescence measurement technique has the potential for on-line characterization of metabolic status of fermentation processes. Many fluorophores present inside the living cells such as NADH + H+, tryptophan, pyridoxine, and riboflavin fluoresce at specific excitation and emission wavelength combinations. Sin...

journal_title：Applied biochemistry and biotechnology

authors： Khanna S,Srivastava AK

更新日期：2009-05-01 00:00:00

abstract：:Steviol glycosides (SVglys) and gibberellins are originated from the shared biosynthesis pathway in Stevia (Stevia rebaudiana Bertoni). In this research, two experiments were conducted to study the opposing effects of external gibberellin (GA3) and Daminozide (a gibberellin inhibitor) on Stevia growth and metabolites....

journal_title：Applied biochemistry and biotechnology

authors： Karimi M,Hashemi J,Ahmadi A,Abbasi A,Pompeiano A,Tavarini S,Guglielminetti L,Angelini LG

更新日期：2015-01-01 00:00:00

abstract：:Polymerization and self-assembly of proteins into nanoaggregates of different sizes and morphologies (nanoensembles or nanofilaments) is a phenomenon that involved problems in various neurodegenerative diseases (medicine) and enzyme instability/inactivity (biotechnology). Thermal polymerization of horse liver alcohol ...

journal_title：Applied biochemistry and biotechnology

authors： Barzegar A,Moosavi-Movahedi AA,Kyani A,Goliaei B,Ahmadian S,Sheibani N

更新日期：2010-02-01 00:00:00

abstract：:Our laboratory has recently developed a device employing immobilized F0F1 adenosine triphosphatase (ATPase) that allows synthesis of adenosine triphosphate (ATP) from adenosine 5'-diphosphate and inorganic phosphate using solar energy. We present estimates of total solar energy received by Earth's land area and demons...

journal_title：Applied biochemistry and biotechnology

authors： Bhattacharya S,Schiavone M,Nayak A,Bhattacharya SK

更新日期：2005-03-01 00:00:00

abstract：:Rice straw hemicellulosic hydrolysate was used as fermentation medium for ethanol production by Pichia stipitis NRRL Y-7124. Shaking bath experiments were initially performed aiming to establish the best initial xylose concentration to be used in this bioconversion process. In the sequence, assays were carried out und...

journal_title：Applied biochemistry and biotechnology

authors： Silva JP,Mussatto SI,Roberto IC

更新日期：2010-11-01 00:00:00

abstract：:Fructose-1,6-bisphosphate (F-1,6-P2) is an allosteric activator of two key enzymes of glycolysis: phosphofructokinase and pyruvate kinase. Regulation of glycolysis in a wild-type Saccharomyces cerevisiae and a recombinant Escherichia coli by a dead-end structural analog of F-1,6-P2 was studied. 2,5-Anhydromannitol (2,...

journal_title：Applied biochemistry and biotechnology

authors： Nghiem NP,Cofer TM

更新日期：2007-05-01 00:00:00

abstract：:The utility of rhamnolipids in industry is currently limited due to the high constraints in its economic production. In this scenario, the novel utility of sodium dodecyl sulphate (SDS) as carbon source could serve as promising cost-effective strategy. Screening of effective SDS biodegraders led to the isolation of Ps...

journal_title：Applied biochemistry and biotechnology

authors： Rebello S,Asok AK,Joseph SV,Joseph BV,Jose L,Mundayoor S,M S J

更新日期：2013-01-01 00:00:00

abstract：:Prostate cancer is one of the most common malignancies among men worldwide. The main aim of the present work was to clarify the advantages of a nanoformulation of ayurvedic herbal plants. Specifically, we assessed the improved anticancer activity of Leucas aspera nanoparticles compared with methanolic crude extract in...

journal_title：Applied biochemistry and biotechnology

authors： Mohan A,Nair SV,Lakshmanan VK

更新日期：2017-04-01 00:00:00

abstract：:Prostate apoptosis response-4 (Par-4), an anticancer protein that interacts with cell surface receptor GRP78, can selectively suppress proliferation and induce apoptosis of cancer cells. The core domain of Par-4 (aa 137-195), designated as SAC, is sufficient to inhibit tumor growth and metastasis without harming norma...

journal_title：Applied biochemistry and biotechnology

authors： Zhang J,Sun A,Dong Y,Wei D

更新日期：2018-04-01 00:00:00

abstract：:Sphaerotilus natans is a sheathed bacterium existing in the activated sludge of wastewater treatment plants. It is one of the filamentous bacteria causing the bulking and foaming difficulties of activated sludge. Isolating the strain and culturing it in an axenic environment could not only provide the metabolic knowle...

journal_title：Applied biochemistry and biotechnology

authors： Liu K,Chua H,Lo WH,Lawford H,Yu PH

更新日期：2002-04-01 00:00:00

abstract：:γ-Aminobutyric acid (γ-GABA) is a non-proteinogenic amino acid, which acts as a major regulator in the central nervous system. Glutamate decarboxylase (namely GAD, EC 4.1.1.15) is known to be an ideal enzyme for γ-GABA production using L-glutamic acid as substrate. In this study, we cloned and expressed GAD gene from ...

journal_title：Applied biochemistry and biotechnology

authors： Xiong Q,Xu Z,Xu L,Yao Z,Li S,Xu H

更新日期：2017-12-01 00:00:00

abstract：:Bioreduction of 1-phenyl-2-propanone to prepare (S)-1-phenyl-2-propanol, a useful pharmaceutical intermediate, was performed with growing cells of Rhodococcus erythropolis JX-021, giving 14 mM (1.9 g/L) product in 99% e.e. at 5 h in the catalysis of 15 mM substrate. The reduction stopped afterwards due to strong inhib...

journal_title：Applied biochemistry and biotechnology

authors： Jin JZ,Li H,Zhang J

更新日期：2010-11-01 00:00:00

abstract：:Amyloglucosidase from Novo (Copenhagen, Denmark) was immobilized in controlled pore silica particles with the silane-glutaraldehyde covalent method. Thermal stability of the free and immobilized enzyme (IE) was determined with 30% (w/v) alpha-amylase liquefied cassava starch, pH 4.5, temperatures from 35 to 75 degrees...

journal_title：Applied biochemistry and biotechnology

authors： Zanin GM,De Moraes FF

更新日期：1998-04-01 00:00:00

abstract：:This article deals with a direct electrochemical method of detecting antigens using new methods of functionalization of gold electrodes. Based on the reacting ability of gold with sulfhydryl groups, three protocols for the fixation of antibodies have been explored. They are based on either the self-assembling properti...

journal_title：Applied biochemistry and biotechnology

authors： Ameur S,Martelet C,Jaffrezic-Renault N,Chovelon JM

更新日期：2000-11-01 00:00:00

abstract：:The effect of surfactant on enzymatic digestibility was investigated during the pretreatment stage. Newspaper was pretreated with an ammonia-hydrogen peroxide mixture on a shaking bath at 40 degrees C and 130 strokes/min for 3 h. Two kinds of nonionic surfactants, NP series and Tween series, were utilized. The effect ...

journal_title：Applied biochemistry and biotechnology

authors： Kim SB,Chun JW

更新日期：2004-04-01 00:00:00