Efficiency of DNA transfection of rat heart myoblast cells H9c2(2-1) by either polyethyleneimine or electroporation.

Abstract:

:Expression of exogenous DNA in vitro is significantly affected by the particular transfection method utilized. In this study, we evaluated the efficiency of two transfection methods, chemically mediated polyethyleneimine (PEI) treatment and physically mediated electroporation, on a rat heart myoblast cell line, H9c2(2-1). After PEI transfection of pPgk-1/EGFP into H9c2(2-1) cells, EGFP expression could be easily detected by fluorospectrometer after 48 h (210 ± 12 RFU) and continued to increase after 72 h (243 ± 14 RFU). However, when H9c2(2-1) cells were transfected by electroporation (200 V, 500 μF, and one pulse), low level EGFP expression was observed after 48 h (49 ± 4 RFU) or 72 h (21 ± 14 RFU). In contrast, the easily transfectable control CHO-K1 cell line displayed a stronger EGFP expression than the H9c2(2-1) cells either by PEI or electroporation transfection. When transfection efficiencies were assayed by flow cytometry after 72 h, 13.6 ± 2.2% of PEI and 10.1 ± 1.5% of electroporation (250 V, 500 μF, and two pulses) transfected cells of H9c2(2-1) expressed EGFP, and PEI-transfected cells appeared to be less damaged (viability 93.6%) as compared to electroporation-transfected cells (39.5%). However, both PEI and electroporation (580 V, 50 Ω, and 50 μF) were effective for transfection of CHO-K1 with a higher efficiency, cell viability, and EGFP expression than H9c2(2-1). Our results indicate that the transfection efficiency of different methods varies among cell lines and that PEI is more efficient than electropolation for transfection of H9c2(2-1) whereas both PEI and electroporation are suitable for CHO-K1 transfection.

authors

Liu YC,Lin WY,Jhang YR,Huang SH,Wu CP,Wu HT

doi

10.1007/s12010-011-9203-5

subject

Has Abstract

pub_date

2011-08-01 00:00:00

pages

1172-82

issue

7

eissn

0273-2289

issn

1559-0291

journal_volume

164

pub_type

杂志文章
  • Polyhydroxybutyrate/Chitosan 3D Scaffolds Promote In Vitro and In Vivo Chondrogenesis.

    abstract::The articular cartilage is an avascular and aneural tissue and its injuries result mostly in osteoarthritic changes and formation of fibrous tissue. Efforts of scientists worldwide are focused on restoration of cartilage with increase in life quality of patients. Novel polymeric polyhydroxybutyrate/chitosan (PCH) poro...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-019-03021-1

    authors: Giretova M,Medvecky L,Petrovova E,Cizkova D,Danko J,Mudronova D,Slovinska L,Bures R

    更新日期:2019-10-01 00:00:00

  • Mixed enzymic reaction--internal diffusion kinetics of nonuniformly distributed immobilized enzymes. The system agarose-micrococcal endonuclease.

    abstract::Two types of (CNBr-activated) Agarose-staphylococcal endonuclease derivatives have been prepared, one with the enzyme uniformly distributed in the support, and the other with the enzyme preferentially bound in the most external part of the support particles; the latter were obtained using agarose of very small pores a...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02798498

    authors: Guisan JM,Serrano J,Melo FV,Ballesteros A

    更新日期:1987-02-01 00:00:00

  • Preserving the activity of cellulase in a batch foam fractionation process.

    abstract::Foam fractionation is one of the low operating-cost techniques for removing proteins from a dilute solution. The initial bulk solution pH and air superficial velocity play an important role in the foam-fractionation process. Denaturation of proteins (enzymes) can occur, however, during the foam-fractionation process f...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:79:1-3:701

    authors: Loha V,Prokop A,Du L,Tanner RD

    更新日期:1999-04-01 00:00:00

  • Effects of pulsed electric field on secondary metabolism of Vitis vinifera L. cv. Gamay Fréaux suspension culture and exudates.

    abstract::Plant cell cultures provide a large potential for the production of secondary metabolites. Through the application of different physical and chemical cell stress factors, we investigated the production of the secondary metabolites in plant cell cultures. The effects of pulsed electric field (PEF) and ethephon on growt...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-010-9146-2

    authors: Cai Z,Riedel H,Thaw Saw NM,Kütük O,Mewis I,Jäger H,Knorr D,Smetanska I

    更新日期:2011-06-01 00:00:00

  • Exploring thermophilic cellulolytic enzyme production potential of Aspergillus fumigatus by the solid-state fermentation of wheat straw.

    abstract::Cellulases can be used for biofuel production to decrease the fuel crises in the world. Microorganisms cultured on lignocellulosic wastes can be used for the production of cellulolytic enzymes at large scale. In the current study, cellulolytic enzyme production potential of Aspergillus fumigatus was explored and optim...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-0796-3

    authors: Mehboob N,Asad MJ,Asgher M,Gulfraz M,Mukhtar T,Mahmood RT

    更新日期:2014-04-01 00:00:00

  • Studies on the Neuroprotection of Osthole on Glutamate-Induced Apoptotic Cells and an Alzheimer's Disease Mouse Model via Modulation Oxidative Stress.

    abstract::In the present study, the neuroprotection of osthole (OST) was confirmed. In L-glutamic acid (L-Glu)-damaged HT22 cells, a 3-h pre-incubation with OST-enhanced cell viability suppressed the apoptosis rate; inhibited the activities of caspase-3, caspase-8, and caspase-9; reduced the over-accumulation of intracellular r...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-019-03101-2

    authors: Chu Q,Zhu Y,Cao T,Zhang Y,Chang Z,Liu Y,Lu J,Zhang Y

    更新日期:2020-02-01 00:00:00

  • Ethanolic fermentation of pentoses in lignocellulose hydrolysates.

    abstract::In the fermentation of lignocellulose hydrolysates to ethanol, two major problems are encountered: the fermentation of the pentose sugar xylose, and the presence of microbial inhibitors. Xylose can be directly fermented with yeasts, such as Pachysolen tannophilus, Candida shehatae, and Pichia stipis, or by isomerizati...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02922595

    authors: Hahn-Hägerdal B,Lindén T,Senac T,Skoog K

    更新日期:1991-04-01 00:00:00

  • Combining enzymatic esterification with conventional alkaline transesterification in an integrated biodiesel process.

    abstract::An integrated biodiesel process that combines enzymatic esterification and alkaline transesterification is suggested. With focus on the enzymatic step, the paper provides proof of concept and suggestions for further process development. Hence, palm fatty acid distillate (PFAD) has been enzymatically converted to fatty...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-010-9095-9

    authors: Brask J,Damstrup ML,Nielsen PM,Holm HC,Maes J,De Greyt W

    更新日期:2011-04-01 00:00:00

  • Biomimetic Mineralization of the Alginate/Gelatin/Calcium Oxalate Matrix for Immobilization of Pectinase: Influence of Matrix on the Pectinolytic Activity.

    abstract::Pectinases catalyze the degradation of pectic substances and are used in several processes, mainly in food and textile industries. In this study, a biomimetic matrix of alginate/gelatin/calcium oxalate (AGOCa) was synthesized for the in situ immobilization via encapsulation of crude pectinase from Aspergillus niger AT...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-016-2050-7

    authors: Bustamante-Vargas CE,de Oliveira D,Valduga E,Venquiaruto LD,Paroul N,Backes GT,Dallago RM

    更新日期:2016-07-01 00:00:00

  • Enhanced secondary metabolite biosynthesis by elicitation in transformed plant root system: effect of abiotic elicitors.

    abstract::Plants generally produce secondary metabolites in nature as a defense mechanism against pathogenic and insect attack. In this study, we applied several abiotic elicitors in order to enhance growth and ginseng saponin biosynthesis in the hairy roots of Panax ginseng. Generally, elicitor treatments were found to inhibit...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:130:1:436

    authors: Jeong GT,Park DH

    更新日期:2006-04-01 00:00:00

  • Production of recombinant porcine interferon alpha using PHB-intein-mediated protein purification strategy.

    abstract::Interferons (IFNs) are involved in the pathogenesis and recovery of viral and other infectious diseases. Recombinant IFNs have been used as anti-infectious agents exhibiting a broad range of antiviral and immunomodulatory properties in both human and domestic animals. In this report, we describe a highly efficient and...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-010-9101-2

    authors: Zhou X,Song Z,Liu X,Jia F,Wang Y

    更新日期:2011-04-01 00:00:00

  • Microencapsulated engineered Lactococcus lactis cells for heterologous protein delivery: preparation and in vitro analysis.

    abstract::This article demonstrates the potential of encapsulated, engineered Lactococcus lactis as a vehicle for the oral delivery of therapeutic proteins. Using alginate-poly-l-lysine-alginate membrane-encapsulated L. lactis engineered to secrete the reporter protein Staphylococcal aureus nuclease, we show comparable viabilit...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-007-0002-y

    authors: Lawuyi B,Chen H,Afkhami F,Kulamarva A,Prakash S

    更新日期:2007-07-01 00:00:00

  • Highly sensitive chemiluminescent analysis of residual bovine serum albumin (BSA) based on a pair of specific monoclonal antibodies and peroxyoxalate-glyoxaline-PHPPA dimer chemiluminescent system in vaccines.

    abstract::Enzyme-linked immunosorbent assay (ELISA), horseradish peroxidase (HRP)-catalyzed fluorescent reaction, and oxalate chemiluminescence analysis have been combined to develop a highly sensitive, simple, and rapid method for analysis of bovine serum albumin (BSA) based on a pair of specific monoclonal antibodies in vacci...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-012-9567-1

    authors: Xue P,Zhang K,Zhang Z,Li Y,Liu F,Sun Y,Zhang X,Song C,Fu A,Jin B,Yang K

    更新日期:2012-03-01 00:00:00

  • Organic and Inorganic Nitrogen Impact Chlorella variabilis Productivity and Host Quality for Viral Production and Cell Lysis.

    abstract::Microalgae have been proposed as a potential feedstock for biofuel production; however, cell disruption is usually required for collection and utilization of cytoplasmic polysaccharides and lipids. Virus infection might be one approach to disrupt the cell wall. The concentration of yeast extract and presence of KNO3 i...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-015-1588-0

    authors: Cheng YS,Labavitch J,VanderGheynst JS

    更新日期:2015-05-01 00:00:00

  • The effects of engineering design on heterogeneous biocatalysis in microchannels.

    abstract::The results of a numerical study of the fundamental interactions of engineering design and micromixing on conversion in packed microchannels are presented. Previously, channel-based microreactors made of molded silicon plastic were designed, fabricated, and experimentally tested. These reactors have enzymes immobilize...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-007-9103-x

    authors: Jones F,Bailey R,Wilson S,Hiestand J

    更新日期:2007-04-01 00:00:00

  • Lipase production by Botryosphaeria ribis EC-01 on soybean and castorbean meals: optimization, immobilization, and application for biodiesel production.

    abstract::The effects of soybean and castorbean meals were evaluated separately, and in combinations at different ratios, as substrates for lipase production by Botryosphaeria ribis EC-01 in submerged fermentation using only distilled water. The addition of glycerol analytical grade (AG) and glycerol crude (CG) to soybean and c...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0309-9

    authors: Andrade MM,Barbosa AM,Bofinger MR,Dekker RF,Messias JM,Guedes CL,Zaminelli T,de Oliveira BH,de Lima VM,Dall'antonia LH

    更新日期:2013-08-01 00:00:00

  • Hemoabzymes. Different strategies for obtaining artificial hemoproteins based on antibodies.

    abstract::Besides existing models of chemical or biotechnological origin for hemoproteins like peroxidases and cytochromes P450, catalytic antibodies (Abs) with a metalloporphyrin cofactor represent a promising alternative route to catalysts tailored for selective oxidation reactions. A brief overview of the literature shows th...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/BF02787712

    authors: Mahy JP,Desfosses B,de Lauzon S,Quilez R,Desfosses B,Lion L,Mansuy D

    更新日期:1998-10-01 00:00:00

  • New model for polymerization of oligomeric alcohol dehydrogenases into nanoaggregates.

    abstract::Polymerization and self-assembly of proteins into nanoaggregates of different sizes and morphologies (nanoensembles or nanofilaments) is a phenomenon that involved problems in various neurodegenerative diseases (medicine) and enzyme instability/inactivity (biotechnology). Thermal polymerization of horse liver alcohol ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-009-8646-4

    authors: Barzegar A,Moosavi-Movahedi AA,Kyani A,Goliaei B,Ahmadian S,Sheibani N

    更新日期:2010-02-01 00:00:00

  • Gold nanomaterials: preparation, chemical modification, biomedical applications and potential risk assessment.

    abstract::Gold nanomaterials (Au NMs) have attracted increasing attention in biomedicine due to their facile preparation, multifunctional modifications, unique optical and electrical properties, and good biocompatibility. The physicochemical properties of Au NMs at nanoscale, like size, shape, surface chemistry, and near field ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s12010-012-9548-4

    authors: Jiang XM,Wang LM,Wang J,Chen CY

    更新日期:2012-03-01 00:00:00

  • Optimization of the production of thermostable endo-beta-1,4 mannanases from a newly isolated Aspergillus niger gr and Aspergillus flavus gr.

    abstract::The aim of this work was to establish optimal conditions for the maximum production of endo-beta-1,4 mannanases using cheaper sources. Eight thermotolerant fungal strains were isolated from garden soil and compost samples collected in and around the Gulbarga University campus, India. Two strains were selected based on...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-008-8250-z

    authors: Kote NV,Patil AG,Mulimani VH

    更新日期:2009-02-01 00:00:00

  • Temperature-dependent behavior of immobilized alkaline phosphatase. I. Role of working conditions and pH during coupling.

    abstract::Alkaline phosphatase from hog intestine was immobilized to controlled-pore glass under various conditions. The specific activity of the enzyme was not diminished by immobilization. The influence of temperature and pH on the behavior and the stability of the immobilized enzyme preparations is discussed and compared to ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02798297

    authors: Pittner F

    更新日期:1982-05-01 00:00:00

  • Cellulases and xylanases production by Penicillium echinulatum grown on sugar cane bagasse in solid-state fermentation.

    abstract::To investigate the production of cellulases and xylanases from Penicillium echinulatum 9A02S1, solid-state fermentation (SSF) was performed by using different ratios of sugar cane bagasse (SCB) and wheat bran (WB). The greatest filter paper activity obtained was 45.82 ± 1.88 U gdm(-1) in a culture containing 6SCB/4WB ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-010-8967-3

    authors: Camassola M,Dillon AJ

    更新日期:2010-11-01 00:00:00

  • In Vivo Evaluation of the Toxic Effect of Ethyl Acetate Extracts of Marine Antibiotic Resistance Pseudomonas Species Derived from the Red Sea.

    abstract::Eighty-nine cultured Pseudomonas species isolated from the sediment and water samples collected from five industrial Red Sea regions that have been affected by petroleum and industry. Genotypic (exoT, exoS, exoU, exoY, lasA, lasB, rhlA, rhlB, Pf1, PAGI-1, -2, and -3) and phenotypic (DNase, elastase, lipase, protease, ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-017-2553-x

    authors: El-Gendy MMAA,Al-Zahrani HAA,Abozinadah NY,El-Bondkly AMA

    更新日期:2018-01-01 00:00:00

  • Multipulse feed strategy for glycerol fed-batch fermentation: a steady-state nonlinear optimization approach.

    abstract::During glycerol fed-batch fermentation, the process could be divided into multiple equal subintervals, and the feed operation was performed in pulse form at the start of each subinterval. Based on the macrokinetic models, the multipulse feed strategy for both glucose and corn steep slurry was determined by a general n...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:95:2:103

    authors: Xie D,Liu D,Zhu H,Liu T

    更新日期:2001-08-01 00:00:00

  • 2D polarization imaging of turbid media.

    abstract::Cancer normally tends to result in the decrease of tissue elasticity; i.e., the cancerous region is more rigid than the normal surrounding areas. This would appear as differences in the distribution of internal birefringence that could be used to improve image contrast between the cancerous and normal tissue structure...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-007-8121-z

    authors: Mathur N,Chaudhary NK,Bharti P

    更新日期:2009-11-01 00:00:00

  • Production of lipid and fatty acids during growth of Aspergillus terreus on hydrocarbon substrates.

    abstract::An Aspergillus terreus, isolated from oil contaminated soil, could degrade a wide range of petroleum hydrocarbons including the immediate oxidation products of hydrocarbons, like alkanols and alkanals. Among all the linear chain carbon substrates, highest growth of 39.1 + or - 3.8 g l(-1) (wet weight) was observed whe...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-009-8669-x

    authors: Kumar AK,Vatsyayan P,Goswami P

    更新日期:2010-03-01 00:00:00

  • Molecular cloning and characterization of the light-harvesting chlorophyll a/b gene from the pigeon pea (Cajanus cajan).

    abstract::Light-harvesting chlorophyll a/b-binding proteins (LHCB) have been implicated in the stress response. In this study, a gene encoding LHCB in the pigeon pea was cloned and characterized. Based on the sequence of a previously obtained 327 bp Est, a full-length 793 bp cDNA was cloned using the rapid amplification of cDNA...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-015-1825-6

    authors: Qiao G,Wen XP,Zhang T

    更新日期:2015-12-01 00:00:00

  • pH-Based Detection of Target Analytes in Diluted Serum Samples Using Surface Plasmon Resonance Immunosensor.

    abstract::Detection of minute quantities of target antigens in serum samples (consisting of a mixture of proteins/biomolecules) can be achieved by enhancement of the capture efficiencies of heterogeneous immunosensors. An important process parameter which affects the capture of target analytes in such immunosensors is the pH of...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-018-2883-3

    authors: Rath D,Kumar S,Panda S

    更新日期:2019-04-01 00:00:00

  • A diverse assemblage of indole-3-acetic acid producing bacteria associate with unicellular green algae.

    abstract::Microalgae have tremendous potential as a renewable feedstock for the production of liquid transportation fuels. In natural waters, the importance of physical associations and biochemical interactions between microalgae and bacteria is generally well appreciated, but the significance of these interactions to algal bio...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-0980-5

    authors: Bagwell CE,Piskorska M,Soule T,Petelos A,Yeager CM

    更新日期:2014-08-01 00:00:00

  • Tannase production by Penicillium purpurogenum PAF6 in solid state fermentation of tannin-rich plant residues following OVAT and RSM.

    abstract::Tannase production by newly isolated Penicillium purpurogenum PAF6 was investigated by 'one variable at a time' (OVAT) approach followed by response surface methodology (RSM). Tannin-rich plant residues were used as supporting solid substrate and sole carbon source and, among them, tamarind seed was found to be the mo...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-012-9547-5

    authors: Jana A,Maity C,Halder SK,Mondal KC,Pati BR,Mohapatra PK

    更新日期:2012-07-01 00:00:00