Efficiency of DNA transfection of rat heart myoblast cells H9c2(2-1) by either polyethyleneimine or electroporation.


:Expression of exogenous DNA in vitro is significantly affected by the particular transfection method utilized. In this study, we evaluated the efficiency of two transfection methods, chemically mediated polyethyleneimine (PEI) treatment and physically mediated electroporation, on a rat heart myoblast cell line, H9c2(2-1). After PEI transfection of pPgk-1/EGFP into H9c2(2-1) cells, EGFP expression could be easily detected by fluorospectrometer after 48 h (210 ± 12 RFU) and continued to increase after 72 h (243 ± 14 RFU). However, when H9c2(2-1) cells were transfected by electroporation (200 V, 500 μF, and one pulse), low level EGFP expression was observed after 48 h (49 ± 4 RFU) or 72 h (21 ± 14 RFU). In contrast, the easily transfectable control CHO-K1 cell line displayed a stronger EGFP expression than the H9c2(2-1) cells either by PEI or electroporation transfection. When transfection efficiencies were assayed by flow cytometry after 72 h, 13.6 ± 2.2% of PEI and 10.1 ± 1.5% of electroporation (250 V, 500 μF, and two pulses) transfected cells of H9c2(2-1) expressed EGFP, and PEI-transfected cells appeared to be less damaged (viability 93.6%) as compared to electroporation-transfected cells (39.5%). However, both PEI and electroporation (580 V, 50 Ω, and 50 μF) were effective for transfection of CHO-K1 with a higher efficiency, cell viability, and EGFP expression than H9c2(2-1). Our results indicate that the transfection efficiency of different methods varies among cell lines and that PEI is more efficient than electropolation for transfection of H9c2(2-1) whereas both PEI and electroporation are suitable for CHO-K1 transfection.


Liu YC,Lin WY,Jhang YR,Huang SH,Wu CP,Wu HT




Has Abstract


2011-08-01 00:00:00












  • Interactive Effects of Growth Regulators, Carbon Sources, pH on Plant Regeneration and Assessment of Genetic Fidelity Using Single Primer Amplification Reaction (SPARS) Techniques in Withania somnifera L.

    abstract::An improved and methodical in vitro shoot morphogenic approach through axillary bud multiplication was established in a drug yielding plant, Withania somnifera L. Effects of plant growth regulators [6-benzyladenine (BA), kinetin (Kin), 2-isopentenyladenine (2iP), and thidiazuron (TDZ)] either singly or in combination ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Fatima N,Ahmad N,Ahmad I,Anis M

    更新日期:2015-09-01 00:00:00

  • Pretreatment of synthetic dairy wastewater using the sophorolipid-producing yeast Candida bombicola.

    abstract::The presence of high strength fats and oils in dairy industry wastewaters poses serious challenges for biological treatment systems, and, therefore, its pretreatment is necessary in order to remove them. In the present study, synthetic dairy wastewater prepared in the laboratory was pretreated using the sophorolipid-p...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Daverey A,Pakshirajan K

    更新日期:2011-03-01 00:00:00

  • Nb-MCM-Type Mesoporous Material Synthesis Using Ionic Solid as Structure-Directing Agent for In Situ Lipase Immobilization.

    abstract::MCM-41 and MCM-48 with niobium were successfully synthesized using 1-tetradecyl-3-methylimidazolium chloride ([C14MI]Cl) as a structure-directing agent. The best Si/Nb molar ratio was chosen (Si/Nb = 20) and the CALB enzyme was immobilized in situ in the synthesized Nb-MCM. SEM micrographs showed the formation of very...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Bordin I,de Aguiar Pedott V,Demaman Oro CE,Junges A,Dallago RM,Mignoni ML

    更新日期:2021-01-06 00:00:00

  • Studies on productivity and characterization of polygalacturonase from Aspergillus giganteus submerged culture using citrus pectin and orange waste.

    abstract::Polygalacturonases are part of the group of enzymes involved in pectin degradation. The aim of this work was to investigate some of the factors affecting polygalacturonase production by an Aspergillus giganteus strain and to characterize this pectinolytic activity. Several carbon sources, both pure substances and natu...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Pedrolli DB,Gomes E,Monti R,Carmona EC

    更新日期:2008-02-01 00:00:00

  • Determination of cyanide using a microbial sensor.

    abstract::A microbial cyanide sensor was prepared, consisting of immobilized Saccharomyces cerevisiae and an oxygen electrode. When the electrode was inserted into a solution containing glucose, the respiration activity of the microorganisms increased. The change in the respiration activity is monitored with the oxygen electrod...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Nakanishi K,Ikebukuro K,Karube I

    更新日期:1996-08-01 00:00:00

  • Development of high-performance and rapid immunoassay for model food allergen lysozyme using antibody-conjugated bacterial magnetic particles and fully automated system.

    abstract::A high-performance and rapid chemiluminescence immunoassay for model food allergen lysozyme, one of the major allergenic components in egg white, using antibody-conjugated bacterial magnetic particles and a fully automated system was developed. This system contains a reaction station, tip rack, and an eight-tip pipett...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Sato R,Takeyama H,Tanaka T,Matsunaga T

    更新日期:2001-04-01 00:00:00

  • Effect of tributylphenyltetraethoxylate on enzyme production of Pleurotus ostreatus.

    abstract::The effect of the addition of the nonionic surfactant tributylphenyltetraethoxylate to culture media on pH and extracellular protein content, and on production of beta-glucosidase, xylanase, laccase, and manganese-dependent and -independent peroxidases by the edible fungus Pleurotus ostreatus was determined. The relat...

    journal_title:Applied biochemistry and biotechnology

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    authors: Ramos C,Morais H,Forgács E,Cserháti T

    更新日期:2003-07-01 00:00:00

  • Prediction of folding stability and degradability of the de novo designed protein MB-1 in cow rumen.

    abstract::The authors have recently reported on the design of a protein (MB-1) enriched in methionine, threonine, lysine, and leucine. The protein is intended to be produced by rumen bacteria, in a way that would provide high producing lactating cows with limiting amino acids. In this report, MB-1 stability in the rumen is asse...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: MacCallum JD,Hefford MA,Omar S,Beauregard M

    更新日期:1997-04-01 00:00:00

  • Biological Response of Osteoblastic and Chondrogenic Cells to Graphene-Containing PCL/Bioactive Glass Bilayered Scaffolds for Osteochondral Tissue Engineering Applications.

    abstract::Graphene-containing 13-93 bioactive glass and poly(ε-caprolactone)-based bilayer, electrically conductive scaffolds were prepared for osteochondral tissue repair. Biological response of osteoblastic MC3T3-E1 and chondrogenic ATDC5 cells to the composite scaffolds was assessed under mono-culture and co-culture conditio...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Deliormanlı AM,Atmaca H

    更新日期:2018-12-01 00:00:00

  • Properties of a thermostable nonspecific fructofuranosidase produced by Cladosporium cladosporioides cells for hydrolysis of Jerusalem artichoke extract.

    abstract::Thermostable invertase (E.C. and inulinase 2,1-beta-D-fructan fructanohydrolase (E.C. activities were produced by Cladosporium cladosporioides grown on sucrose, inulin, yam extract, or Jerusalem artichoke. The ratio I (inulinase)/S(invertase) activity was between 0.31 and 0.36. Both activities had h...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Ferreira MS,De Andrade AV,Kennedy JF

    更新日期:1991-10-01 00:00:00

  • Optimization of fermentation conditions for production of glycopeptide antibiotic vancomycin by Amycolatopsis orientalis.

    abstract::Glycopeptides produced by Streptomyces species are the drugs used against beta-lactam drug-resistant staphylococcal infections, and vancomycin is important among them. Increased prevalence of resistant strains increased the usage of vancomycin worldwide and also promoted attempts for indigenous production. The optimum...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Padma PN,Rao AB,Yadav JS,Reddy G

    更新日期:2002-07-01 00:00:00

  • Hen ovomucoid-agarose: A new conjugate for the isolation by affinity chromatography of UDP-galactose: Glycoprotein galactosyl-transferase.

    abstract::A new conjugate for the affinity chromatography of UDP-galactose:glycoprotein galactosyltransferase has been synthesized by coupling hen ovomucoid, a ligand similar to the acceptor substrate, to agarose.The hen ovomucoid-Sepharose conjugate binds galactosyl transferase more tightly that other acceptor-Sepharose conjug...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Tadolini B,Hakim G

    更新日期:1981-09-01 00:00:00

  • Recycling of NAD(+) using coimmobilized alcohol dehydrogenase andE. coli.

    abstract::The use of immobilized enzymes has opened the possibility of large scale utilization of NAD(+)-linked dehydrogenases, but the applications of this technique were limited by the necessity of providing the large amounts of NAD(+) required by its stoichiometric consumption in the reaction. After immobilization of alcohol...

    journal_title:Applied biochemistry and biotechnology

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    authors: Burstein C,Ounissi H,Legoy MD,Gellf G,Thomas D

    更新日期:1981-12-01 00:00:00

  • The oxidative stress of Phanerochaete chrysosporium against lead toxicity.

    abstract::Among the technologies for heavy metal remediation, bioremediation technology has gained extensive attention because of its low processing costs and high efficiency. The white-rot fungus Phanerochaete chrysosporium (P. chrysosporium) which has a good tolerance to heavy metals has been widely used in the heavy metal bi...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Wan J,Zeng G,Huang D,Huang C,Lai C,Li N,Wei Z,Xu P,He X,Lai M,He Y

    更新日期:2015-02-01 00:00:00

  • Screening thermotolerant white-rot fungi for decolorization of wastewaters.

    abstract::To select a thermotolerant fungal strain for decolorization of wastewaters, ligninolytic enzyme production (lignin peroxidase, manganese peroxidase [MnP], and laccase), decolorization, and removal of total phenol and chemical oxygen demand (COD) were detected. Thirty-eight fungal strains were studied for enzyme produc...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Chairattanamanokorn P,Imai T,Kondo R,Ukita M,Prasertsan P

    更新日期:2006-03-01 00:00:00

  • Biodegradation of crude oil from the BP oil spill in the marsh sediments of southeast Louisiana, USA.

    abstract::The significant challenges presented by the April 20, 2010 explosion, sinking, and subsequent oil spill of the Deepwater Horizon drilling platform in Canyon Block 252 about 52 miles southeast of Venice, LA, USA greatly impacted Louisiana's coastal ecosystem including the sea food industry, recreational fishing, and to...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Boopathy R,Shields S,Nunna S

    更新日期:2012-07-01 00:00:00

  • Shoot organogenesis from root-derived callus of Rhinacanthus nasutus (L.) Kurz. and assessment of clonal fidelity of micropropagted plants using RAPD analysis.

    abstract::An efficient regeneration system was established for an ethnomedicinal shrub Rhinacanthus nasutus from root-derived callus organogenesis. The root segments were cultured on MS medium supplemented with various concentrations of Kn (1.0-4.0 μM) alone or in combination with IBA (0.2-0.6 μM) or 2, 4-D (0.5-1.5 μM). The op...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Cheruvathur MK,Thomas TD

    更新日期:2014-02-01 00:00:00

  • The new application of biosorption properties of Enteromorpha prolifera.

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    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Michalak I,Chojnacka K

    更新日期:2010-03-01 00:00:00

  • Identification of heat-related ESTs in moth bean through suppression subtraction hybridization.

    abstract::Moth bean (Vigna aconitifolia (Jacq.) Marechal), an important grain-legume crop grown in hot desert regions of Thar, under scorching sun rays, was investigated for heat tolerance at molecular level. In the present study, we constructed a forward suppression subtractive hybridization (SSH) cDNA library of heat tolerant...

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    authors: Gurjar K,Rampuria S,Joshi U,Palit P,Bhatt KV,Mohapatra T,Sharma R

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  • Cloning and Characterization of Cold-Adapted α-Amylase from Antarctic Arthrobacter agilis.

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    authors: Kim SM,Park H,Choi JI

    更新日期:2017-03-01 00:00:00

  • Lactase production by solid-state cultivation of Kluyveromyces marxianus CDBBL 278 on an inert support: effect of inoculum, buffer, and nitrogen source.

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    journal_title:Applied biochemistry and biotechnology

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    authors: Tovar-Castro L,García-Garibay M,Saucedo-Castañeda G

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  • Purification and biochemical characterization of a highly thermostable xylanase from Actinomadura sp. strain Cpt20 isolated from poultry compost.

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    journal_title:Applied biochemistry and biotechnology

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    authors: Taibi Z,Saoudi B,Boudelaa M,Trigui H,Belghith H,Gargouri A,Ladjama A

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  • Isolation and screening of microorganisms for R-(+)-limonene and (-)-beta-pinene biotransformation.

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    journal_title:Applied biochemistry and biotechnology

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    authors: Rottava I,Cortina PF,Grando CE,Colla AR,Martello E,Cansian RL,Toniazzo G,Treichel H,Antunes OA,Oestreicher EG,de Oliveira D

    更新日期:2010-10-01 00:00:00

  • Importance of C-Terminal Extension in Thermophilic 1,4-α-Glucan Branching Enzyme from Geobacillus thermoglucosidans STB02.

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    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Ban X,Li C,Zhang Y,Gu Z,Cheng L,Hong Y,Li Z

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  • MS analysis and molecular characterization of Botrytis cinerea protease Prot-2. Use in bioactive peptides production.

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    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Abidi F,Aissaoui N,Gaudin JC,Chobert JM,Haertlé T,Marzouki MN

    更新日期:2013-05-01 00:00:00

  • Rhizopus oryzae lipase immobilized on hierarchical mesoporous silica supports for transesterification of rice bran oil.

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    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Ramachandran P,Narayanan GK,Gandhi S,Sethuraman S,Krishnan UM

    更新日期:2015-03-01 00:00:00

  • Heterologous expression of a Nelumbo nucifera phytochelatin synthase gene enhances cadmium tolerance in Arabidopsis thaliana.

    abstract::Phytochelatin synthase (PCS) is a key enzyme involved in the synthesis of phytochelatins, which are thought to play important roles in heavy metal detoxification. The sacred lotus (Nelumbo nucifera), one of the most popular ornamental species, has been shown to be a potential phytoremediator of heavy metal polluted wa...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Liu Z,Gu C,Chen F,Yang D,Wu K,Chen S,Jiang J,Zhang Z

    更新日期:2012-02-01 00:00:00

  • Exploring the cause of oseltamivir resistance against mutant H274Y neuraminidase by molecular simulation approach.

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    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Karthick V,Shanthi V,Rajasekaran R,Ramanathan K

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  • Phytodegradation potential of Erythrina crista-galli L., Fabaceae, in petroleum-contaminated soil.

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    pub_type: 杂志文章


    authors: de Farias V,Maranho LT,de Vasconcelos EC,da Silva Carvalho Filho MA,Lacerda LG,Azevedo JA,Pandey A,Soccol CR

    更新日期:2009-04-01 00:00:00

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    abstract::A cascade reaction combining the enzymatic hydrolysis of Penicillin G potassium salt (PGK) with the kinetically controlled enzymatic coupling of in situ formed 6-aminopenicillanic acid (6-APA) with p-hydroxyphenylglycine methyl ester (D-HPGM) to give amoxicillin as the final product by using a single enzyme has been d...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Wu Q,Chen CX,Du LL,Lin XF

    更新日期:2010-04-01 00:00:00