Abstract:
:Laccases are enzymes belonging to the Oxidoreductases class. These enzymes may be good biocatalysts for different processes, at laboratory and industrial levels. A successful use at industrial scale demands a higher stability of the enzyme. As an easy way to obtain longer life biocatalysts, the immobilization process is recommended. Thus, the paper presents different ways of obtaining new biocatalysts by a laccase covalent immobilization on a macroporous carrier based on poly(vinyl alcohol) cryogel. Different procedures of covalent immobilization are described, the newly obtained biocatalysts being characterized. According to the experimental data, the stability of the immobilized enzyme increased and the pH profile changed, compared with those of the free enzyme.
journal_name
Appl Biochem Biotechnoljournal_title
Applied biochemistry and biotechnologyauthors
Stanescu MD,Fogorasi M,Shaskolskiy BL,Gavrilas S,Lozinsky VIdoi
10.1007/s12010-009-8755-0subject
Has Abstractpub_date
2010-04-01 00:00:00pages
1947-54issue
7eissn
0273-2289issn
1559-0291journal_volume
160pub_type
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