New potential biocatalysts by laccase immobilization in PVA Cryogel type carrier.

Abstract:

:Laccases are enzymes belonging to the Oxidoreductases class. These enzymes may be good biocatalysts for different processes, at laboratory and industrial levels. A successful use at industrial scale demands a higher stability of the enzyme. As an easy way to obtain longer life biocatalysts, the immobilization process is recommended. Thus, the paper presents different ways of obtaining new biocatalysts by a laccase covalent immobilization on a macroporous carrier based on poly(vinyl alcohol) cryogel. Different procedures of covalent immobilization are described, the newly obtained biocatalysts being characterized. According to the experimental data, the stability of the immobilized enzyme increased and the pH profile changed, compared with those of the free enzyme.

authors

Stanescu MD,Fogorasi M,Shaskolskiy BL,Gavrilas S,Lozinsky VI

doi

10.1007/s12010-009-8755-0

subject

Has Abstract

pub_date

2010-04-01 00:00:00

pages

1947-54

issue

7

eissn

0273-2289

issn

1559-0291

journal_volume

160

pub_type

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