Characterization of a recombinant flocculent Saccharomyces cerevisiae strain that co-ferments glucose and xylose: II. influence of pH and acetic acid on ethanol production.

abstract：:Equipment and procedures were developed to study the conversion of lignocellulosic biomass to carboxylic acids using high-solids countercurrent fermentations. Countercurrent fermentations of cattle manure yielded a rapid fermentation (maximum 2.98 g of total acid/[L x d]) with high acid concentrations (maximum of 32.5...

journal_title：Applied biochemistry and biotechnology

authors： Ross MK,Holtzapple MT

更新日期：2001-05-01 00:00:00

abstract：:The recombinant Escherichia coli gamma-glutamyltranspeptidase (EcGGT) was immobilized in Ca-alginate-kappa-carrageenan beads. Effects of alginate concentration, amount of loading enzyme, and bead size on the entrapped activity were investigated. Optimum alginate concentration for EcGGT immobilization was found to be 2...

journal_title：Applied biochemistry and biotechnology

authors： Hung CP,Lo HF,Hsu WH,Chen SC,Lin LL

更新日期：2008-08-01 00:00:00

abstract：:To achieve targeted distribution of anticancer drugs with sustained activity, ferromagnetic ethylcellulose microcapsules containing an anticancer drug, mitomycin C (FM-MMC-mc), were prepared by a method based on phase separation principles. Two prototypes of FM-MMC-mc were made: one with the drug as the core and zinc ...

journal_title：Applied biochemistry and biotechnology

authors： Kato T,Nemoto R,Mori H,Abe R,Unno K,Goto A,Murota H,Harada M,Homma M

更新日期：1984-01-01 00:00:00

abstract：:Observation of the ultrastructural and intracellular chemical changes of CaCl2-tolerant strains is important both for understanding their adaptation mechanism under high salt stress and for providing theoretical basis of their application in treating high-CaCl2 wastewaters. A novel strain V 430 isolated by us has been...

journal_title：Applied biochemistry and biotechnology

authors： Xin X,Wang Y

更新日期：2007-09-01 00:00:00

abstract：:Formation of methane in the anodic chamber of a microbial fuel cell (MFC) indicates an energy inefficiency in electricity generation as the energy required for electrogenesis gets redirected to methanogenesis. The hypothesis of this research is that inhibition of methanogenesis in the mixed anaerobic anodic inoculum i...

journal_title：Applied biochemistry and biotechnology

authors： Tholia V,Neethu B,Bhowmick GD,Ghangrekar MM

更新日期：2020-11-16 00:00:00

abstract：:Proteases are some of the most important industrial enzymes, and one of their main applications is for the production of cheese in the dairy industry. Due to a shortage of animal rennet, microbial coagulant proteases are being sought. In this work, the production of microbial rennet from Thermomucor indicae-seudaticae...

journal_title：Applied biochemistry and biotechnology

authors： Silva BL,Geraldes FM,Murari CS,Gomes E,Da-Silva R

更新日期：2014-02-01 00:00:00

abstract：:Aliphatic chlorinated compounds, such as trichloroethylene (TCE) and tetrachloroethylene (PCE), are major contaminants of ground water. A single-pass packed-bed bioreactor was utilized to study the biodegradation of organic waste mixtures consisting of PCE, TCE, and other short-chain chlorinated organics. The bioreact...

journal_title：Applied biochemistry and biotechnology

authors： Lackey LW,Phelps TJ,Bienkowski PR,White DC

更新日期：1993-04-01 00:00:00

abstract：:Foam fractionation is one of the low operating-cost techniques for removing proteins from a dilute solution. The initial bulk solution pH and air superficial velocity play an important role in the foam-fractionation process. Denaturation of proteins (enzymes) can occur, however, during the foam-fractionation process f...

journal_title：Applied biochemistry and biotechnology

authors： Loha V,Prokop A,Du L,Tanner RD

更新日期：1999-04-01 00:00:00

abstract：:Enzymatic hydrolysis of cellulosic raw materials to produce nutrient broths for microbiological synthesis of ethanol and other valuable products is an important field of modern biotechnology. Biotechnological processing implies the selection of an effective pretreatment technique for raw materials. In this study, the ...

journal_title：Applied biochemistry and biotechnology

authors： Denisova MN,Makarova EI,Pavlov IN,Budaeva VV,Sakovich GV

更新日期：2016-03-01 00:00:00

abstract：:The gate surface of an ion-sensitive field effect transistor (ISFET) was modified with Langmuir-Blodgett (LB) film composed of fatty acid or crown ether amphiphiles to examine their potentiometric response to H+ and K+ ions. The results demonstrate the possible use of the lipid films for preparing ISFET ion sensors. ...

journal_title：Applied biochemistry and biotechnology

authors： Osa T

更新日期：1993-04-01 00:00:00

abstract：:Concentrated dilute acid hydrolysate was obtained from hot water extracts of Acer saccharum (sugar maple) and was fermented to ethanol by Pichia stipitis in a 1.3-L-benchtop bioreactor. The conditions under which the highest ethanol yield was achieved were when the air flow rate was set to 100 cm(3) and the agitation ...

journal_title：Applied biochemistry and biotechnology

authors： Shupe AM,Liu S

更新日期：2012-09-01 00:00:00

abstract：:The 1,3-dihydroxyacetone (DHA)-overproducing mutant of Gluconobacter oxydans was screened via UV mutagenesis to enhance the DHA production, and the DHA fermentation condition was optimized using the dissolved oxygen (DO) control strategy. The stable mutant G. oxydans ZJB11001 exhibits high DHA productivity and can tol...

journal_title：Applied biochemistry and biotechnology

authors： Hu ZC,Zheng YG

更新日期：2011-11-01 00:00:00

abstract：:Nanotechnology is an emerging technology which is an amalgamation of different aspects of science and technology that includes disciplines such as electrical engineering, mechanical engineering, biology, physics, chemistry, and material science. It has potential in the fields of information and communication technolog...

journal_title：Applied biochemistry and biotechnology

authors： Chakraborty M,Jain S,Rani V

更新日期：2011-11-01 00:00:00

abstract：:A newly isolated bacterial strain, Bacillus sp. MX47, was actively producing extracellular xylanase only in xylan-containing medium. The xylanase was purified from the culture broth by two chromatographic steps. The xylanase had an apparent molecular weight of 26.4 kDa with an NH(2)-terminal sequence (Gln-Gly-Gly-Asn-...

journal_title：Applied biochemistry and biotechnology

authors： Chi WJ,Park DY,Chang YK,Hong SK

更新日期：2012-10-01 00:00:00

abstract：:The present study was undertaken to evaluate the effect of initial temperature treatment on phytochemical and antioxidant potential of commercially important plant Azadirachta indica A. Juss. The leaves were differentially treated after harvest at temperatures 4, -20, and 110 °C and at room temperature, separately. It...

journal_title：Applied biochemistry and biotechnology

authors： Vats S

更新日期：2016-02-01 00:00:00

abstract：:Vegetable and fruit waste (VFW) is becoming a heavy burden of municipal waste disposal because of its huge amount, but it is a potentially valuable resource that can be developed into high value products such as methane. Conventional anaerobic digestion processes are not suitable for solving the problem of easy acidif...

journal_title：Applied biochemistry and biotechnology

authors： Liu WY,Liao B

更新日期：2019-05-01 00:00:00

abstract：:The bioaugmentation of petroleum-contaminated soil using Enterobacter cloacae was profiled from the evolution of microbial community, soil dehydrogenase activity, to the degradation of petroleum contaminants. The seeding and proliferation of inoculant and the consequential microbial community were monitored by denatur...

journal_title：Applied biochemistry and biotechnology

authors： Wu Z,Dong H,Zou L,Lu D,Liu Z

更新日期：2011-08-01 00:00:00

abstract：:The objective of the present work was to evaluate the metabolic effects induced by the initial glucose concentration (IGC) on the cultivation of Streptococcus zooepidemicus for the production of hyaluronic acid (HA). These effects were monitored along non-controlled pH cultivations, carried out in 250-mL Erlenmeyer fl...

journal_title：Applied biochemistry and biotechnology

authors： Pires AM,Santana MH

更新日期：2010-11-01 00:00:00

abstract：:Phytochelatin synthase (PCS) is a key enzyme involved in the synthesis of phytochelatins, which are thought to play important roles in heavy metal detoxification. The sacred lotus (Nelumbo nucifera), one of the most popular ornamental species, has been shown to be a potential phytoremediator of heavy metal polluted wa...

journal_title：Applied biochemistry and biotechnology

authors： Liu Z,Gu C,Chen F,Yang D,Wu K,Chen S,Jiang J,Zhang Z

更新日期：2012-02-01 00:00:00

abstract：:Lysozyme was immobilized by two different methods in two different ways in order to obtain a preparation with an activity as high as possible toward a macromolecular substrate. The enzyme was bound as a Schiff base to a silicate carrier by using oxidized dextrans of different lengths as spacer and also was bound to co...

journal_title：Applied biochemistry and biotechnology

authors： Moser I,Dworsky P,Pittner F

更新日期：1988-12-01 00:00:00

abstract：:The yeast Saccharomyces cerevisiae has been used to efficiently produce L-malic acid from fumaric acid. Fumarase is responsible for the reversible conversion of fumaric and L-malic acids in the TCA cycle. To investigate the function of mitochondrial and cytoplasmic fumarase isoenzymes in L-malic acid bioconversion, a ...

journal_title：Applied biochemistry and biotechnology

authors： Wang X,Gong CS,Tsao GT

更新日期：1998-04-01 00:00:00

abstract：:Aldehyde dehydrogenase (E.C. 1.2.1.x) can catalyze detoxification of acetaldehydes. A novel acetaldehyde dehydrogenase (istALDH) from the non-Saccharomyces yeast Issatchenkia terricola strain XJ-2 has been previously characterized. In this work, Lactococcus lactis with the NIsin Controlled Expression (NICE) System was...

journal_title：Applied biochemistry and biotechnology

authors： Lyu Y,LaPointe G,Zhong L,Lu J,Zhang C,Lu Z

更新日期：2018-02-01 00:00:00

abstract：:The abilities of lignin peroxidase (LIP) and manganese peroxidase (MNP) from Phanerochaete chrysosporium to degrade an insoluble hardwood lignin in vitro in aqueous media were tested. Neither LIP nor MNP appreciably changed the mass or lignin content, although both produced small amounts of unique solubilized lignin f...

journal_title：Applied biochemistry and biotechnology

authors： Thompson DN,Hames BR,Reddy CA,Grethlein HE

更新日期：1998-04-01 00:00:00

abstract：:Catechol-2, 3-dioxygenase (C23O) from Pseudomonas sp. CGMCC2953 identified in our laboratory, which is one of the key enzymes responsible for phenanthrene biodegradation, was expected to get better characteristics tolerant to environment for its further application. With the aim of improving the enzyme properties by i...

journal_title：Applied biochemistry and biotechnology

authors： Wei J,Zhou Y,Xu T,Lu B

更新日期：2010-09-01 00:00:00

abstract：:Further characterization of an aspartyl protease from Mucor bacilliformis with milk-clotting activity was performed. An extinction coefficient, epsilon 278 cm = 1.61 mL/mg/cm, a molecular mass of 35,400 Da and a pI of 5.2 were determined. Proteolytic activity and kinetic parameters were evaluated by using the hexapept...

journal_title：Applied biochemistry and biotechnology

authors： Venera GD,Machalinski C,Zumárraga H,Biscoglio de Jiménez Bonino MJ

更新日期：1997-12-01 00:00:00

abstract：:The nuclear receptor Rev-erbβ is a potent transcriptional factor whose functional study has been limited by the lack of suitable antibodies against it. To better understand Rev-erbβ's biological roles, we generated five hybridoma cell lines secreting antibodies against human Rev-erbβ in mice immunized with the purifie...

journal_title：Applied biochemistry and biotechnology

authors： Chen F,Li Y,Zhao J,Mao Q,Xia H

更新日期：2017-07-01 00:00:00

abstract：:Glutathione (γ-glutamyl-L-cysteinylglycine, GSH) is a pharmaceutical compound often used in food additives and the cosmetics industry. GSH can be produced biologically from L-glutamic acid, L-cysteine, and glycine through an enzymatic process traditionally involving two sequential adenosine triphosphate (ATP)-dependen...

journal_title：Applied biochemistry and biotechnology

authors： Jiang Y,Tao R,Shen Z,Sun L,Zhu F,Yang S

更新日期：2016-12-01 00:00:00

abstract：:Proteases, lipases, amylases, and cellulases are enzymes used in detergent formulation to improve the detergency. The amylases are specifically supplemented to the detergent to digest starchy stains. Most of the solid and liquid detergents that are currently manufactured contain alkaline enzymes. The advantages of usi...

journal_title：Applied biochemistry and biotechnology

authors： Niyonzima FN,More SS

更新日期：2014-10-01 00:00:00

abstract：:In the fermentation of lignocellulose hydrolysates to ethanol, two major problems are encountered: the fermentation of the pentose sugar xylose, and the presence of microbial inhibitors. Xylose can be directly fermented with yeasts, such as Pachysolen tannophilus, Candida shehatae, and Pichia stipis, or by isomerizati...

journal_title：Applied biochemistry and biotechnology

authors： Hahn-Hägerdal B,Lindén T,Senac T,Skoog K

更新日期：1991-04-01 00:00:00

abstract：:In this study, an efficient procedure was developed for callus induction and regeneration of kiwifruit (Actinidia deliciosa) using different organs of shoots developed under in vitro conditions. Effects of explants source and media (M(1), 1.0 mg l(-1) BA + 2.0 mg l(-1) 2,4-D-M(2), 1.0 mg l(-1) NAA + 2.0 mg l(-1) 2,4-D...

journal_title：Applied biochemistry and biotechnology

authors： Akbaş F,Işikalan C,Namli S

更新日期：2009-08-01 00:00:00