Synthesis of Chitin Oligosaccharides Using Dried Stenotrophomonas maltophilia Cells Containing a Transglycosylation Reaction-Catalyzing β-N-Acetylhexosaminidase as a Whole-Cell Catalyst.

abstract：:The enzymatic alcoholysis of soybean oil with methanol and ethanol was investigated using a commercial, immobilized lipase (Lipozyme RMIM). The effect of alcohol (methanol or ethanol), enzyme concentration, molar ratio of alcohol to soybean oil, solvent, and temperature on biodiesel production was determined. The best...

journal_title：Applied biochemistry and biotechnology

authors： Bernardes OL,Bevilaqua JV,Leal MC,Freire DM,Langone MA

更新日期：2007-04-01 00:00:00

abstract：:A proteinaceous glycopeptide was isolated from the posterior salivary gland (PSG) of Sepia pharaonis by gel (Sephadex G-100) filtration chromatography and purified by reversed-phase high-performance liquid chromatography (RP-HPLC). Among the collected fractions, fraction 12 showed a retention time (RT) of 31 min. The ...

journal_title：Applied biochemistry and biotechnology

authors： Karthik R,Saravanan R,Ebenezar KK,Sivamalai T

更新日期：2015-02-01 00:00:00

abstract：:Dimethyl sulfide (DMS) is one of the sulfurous pollutants present in the waste gas generated from the pulp and paper industry. DMS has environmental health implications; therefore, it is necessary to treat the waste gas containing DMS prior to discharge into the environment. A bench-scale biofilter was operated in the...

journal_title：Applied biochemistry and biotechnology

authors： Giri BS,Juwarkar AA,Satpute DB,Mudliar SN,Pandey RA

更新日期：2012-07-01 00:00:00

abstract：:Increased demand for ethanol as a fuel additive has resulted in dramatic growth in ethanol production. Ethanol is produced from corn by either wet milling or dry-grind processing. In wet milling, the corn kernel is fractionated into different components, resulting in several coproducts. Wet-milling plants are capital ...

journal_title：Applied biochemistry and biotechnology

authors： Rausch KD,Belyea RL

更新日期：2006-01-01 00:00:00

abstract：:The effects of oxygen limitation on xylose fermentation of Neurospora crassa AS3.1602 were studied using batch cultures. The maximum yield of ethanol was 0.34 g/g at oxygen transfer rate (OTR) of 8.4 mmol/L.h. The maximum yield of xylitol was 0.33 g/g at OTR of 5.1 mmol/L.h. Oxygen limitation greatly affected mycelia ...

journal_title：Applied biochemistry and biotechnology

authors： Zhang Z,Qu Y,Zhang X,Lin J

更新日期：2008-03-01 00:00:00

abstract：:Exopolysaccharide of Lachnum YM130 (LEP) was purified by diethylaminoethyl cellulose 52 and Sepharose CL-6B column chromatography. LEP-2a was identified to be a homogeneous component with an average molecular weight of 1.31 × 106 Da, which was consisted of mannose and galactose in a molar ratio of 3.8:1.0. The structu...

journal_title：Applied biochemistry and biotechnology

authors： Xu P,Yuan R,Hou G,Li J,Ye M

更新日期：2018-06-01 00:00:00

abstract：:Carnosol and carnosic acid are polyphenolic compounds found in rosemary and sage with known anti-oxidant, anti-inflammatory, and anti-microbial properties. Here, we addressed the potential use of carnosol and carnosic acid for in vitro bone tissue engineering applications, specifically depending on their cytotoxic eff...

journal_title：Applied biochemistry and biotechnology

authors： Karadas O,Mese G,Ozcivici E

更新日期：2019-06-01 00:00:00

abstract：:Changes in protein glycosylation owing to changes in environmental conditions are not well understood. To better understand these relationships, methods to quantify controlling factors are needed. Because enzymes are translated from genes, the ability to quantify gene expression levels for glycosylation-related enzyme...

journal_title：Applied biochemistry and biotechnology

authors： Clark KJ,Harcum SW,Griffiths J,Bailey KM

更新日期：2005-06-01 00:00:00

abstract：:A simple staged model for the protein foam fractionation process is proposed in this article. This simplified model does not detail the complex foam structure and gas-liquid hydrodynamics in the foam phase but, rather, is built on the conventional theoretical stage concept considering upward bubbles with entrained liq...

journal_title：Applied biochemistry and biotechnology

authors： Du L,Loha V,Tanner RD

更新日期：2000-04-01 00:00:00

abstract：:Sweet sorghum (Sorghum sp.) has high biomass yield. Hydrolysis of lignocellulosic sweet sorghum bagasse (SSB) to fermentable sugar could be useful for manufacture of biofuel or other fermentation products. Pretreatment of lignocellulosic biomass to degrade lignin before enzymatic hydrolysis is a key step. Fungal pretr...

journal_title：Applied biochemistry and biotechnology

authors： Mishra V,Jana AK

更新日期：2017-09-01 00:00:00

abstract：:Phytases act on phytic acid, an antinutrient factor present in animal feeds, and release inorganic phosphate. We optimized the production parameters for phytase production using Thermoascus aurantiacus (TUB F 43), a thermophilic fungal culture, by submerged fermentation. A semisynthetic medium containing glucose, star...

journal_title：Applied biochemistry and biotechnology

authors： Nampoothiri KM,Tomes GJ,Roopesh K,Szakacs G,Nagy V,Soccol CR,Pandey A

更新日期：2004-07-01 00:00:00

abstract：:The enhancement of the biomass productivity of Escherichia coli cells harbouring the truncated 903 bp gene designated as glycoside hydrolase family 43 (GH43) from Clostridium thermocellum showing hemicellulase activity along with its further use in simultaneous saccharification and fermentation (SSF) process is descri...

journal_title：Applied biochemistry and biotechnology

authors： Das SP,Ravindran R,Ahmed S,Das D,Goyal D,Fontes CM,Goyal A

更新日期：2012-07-01 00:00:00

abstract：:A biosensor for ascorbic acid based on enzyme kinetics of ascorbate oxidase (E.C.1.10.3.3) was developed. The enzyme was extracted from Cucurbita maxima, or jerimun and immobilized by covalent bounding, using glutaradehyde as a bifunctional agent, on alkylamine glass beads, with and without enzyme active site protecti...

journal_title：Applied biochemistry and biotechnology

authors： Marques ET Jr,Lima Filho JL

更新日期：1992-01-01 00:00:00

abstract：:Research on biomass pretreatment to enhance enzymatic digestibility has been done for more than decades, but a viable continuous pretreatment method needs to be developed. Extrusion has the potential to be a viable continuous pretreatment method. This study investigated the effect of compression ratio (2:1 and 3:1), s...

journal_title：Applied biochemistry and biotechnology

authors： Karunanithy C,Muthukumarappan K

更新日期：2010-11-01 00:00:00

abstract：:Although the use of lipases as biocatalysts has frequently been proposed, it is yet scarcely being implemented in industrial processes. This is mainly due to the difficulties associated with the discovery and engineering of new enzymes and the lack of versatile screening methods. In this study, we screened the availab...

journal_title：Applied biochemistry and biotechnology

authors： Roh C,Schmid RD

更新日期：2013-12-01 00:00:00

abstract：:Endoglucanase 5 (EG5) has been isolated from the strain of E. coli TG1 harboring recombinant plasmid pCU108, which contains the cel5 gene of C. thermocellum. The enzyme has been produced with 98-fold purification and a final yield of 27% by using subsequent twofold high performance ion-exchange chromatography on Mono ...

journal_title：Applied biochemistry and biotechnology

authors： Mosolova TP,Kalyuzhnyi SV,Varfolomeyev SD,Velikodvorskaya GA

更新日期：1993-07-01 00:00:00

abstract：:Polymerization and self-assembly of proteins into nanoaggregates of different sizes and morphologies (nanoensembles or nanofilaments) is a phenomenon that involved problems in various neurodegenerative diseases (medicine) and enzyme instability/inactivity (biotechnology). Thermal polymerization of horse liver alcohol ...

journal_title：Applied biochemistry and biotechnology

authors： Barzegar A,Moosavi-Movahedi AA,Kyani A,Goliaei B,Ahmadian S,Sheibani N

更新日期：2010-02-01 00:00:00

abstract：:We have developed a substrate-induced growth response (SIGR) method for quantifying activity and population dynamics of microorganisms involved in bioremediation processes in soil and bioreactors. The biomass of organisms that can mineralize a given chemical can be estimated based on the concentration of that chemical...

journal_title：Applied biochemistry and biotechnology

authors： Schmidt SK,Colores GM,Hess TF,Radehaus PM

更新日期：1995-07-01 00:00:00

abstract：:In this study, the gene encoding an α-amylase from a psychrophilic Arthrobacter agilis PAMC 27388 strain was cloned into a pET-28a(+) vector and heterologously expressed in Escherichia coli BL21(DE3). The recombinant α-amylase with a molecular mass of about 80 kDa was purified by using Ni2+-NTA affinity chromatography...

journal_title：Applied biochemistry and biotechnology

authors： Kim SM,Park H,Choi JI

更新日期：2017-03-01 00:00:00

abstract：:The host-pathogen interaction and involvement of calcium (Ca2+) signaling in tuberculosis infection is crucial and plays a significant role in pathogenesis. Ca2+ is known as a ubiquitous second messenger that could control multiple processes and is included in cellular activities like division, motility, stress respon...

journal_title：Applied biochemistry and biotechnology

authors： Sharma S,Meena LS

更新日期：2017-02-01 00:00:00

abstract：:A simple, sensitive aptamer-based biosensor for the detection of phenylalanine is developed using the electrochemical transduction method. For this proposed aptasensor, a 5-thiol-terminated aptamer is covalently attached onto a gold electrode. At the first time, the electrode was evaluated as an electrochemical aptase...

journal_title：Applied biochemistry and biotechnology

authors： Omidinia E,Shadjou N,Hasanzadeh M

更新日期：2014-02-01 00:00:00

abstract：:The mutant strain designated as ART18, obtained from the wild-type strain Clostridium acetobutylicum PW12 treated by atmospheric and room temperature plasma, showed higher solvent tolerance and butanol production than that of the wild-type strain. The production of butanol was 11.3 ± 0.5 g/L, 31 % higher than that of ...

journal_title：Applied biochemistry and biotechnology

authors： Li HG,Luo W,Wang Q,Yu XB

更新日期：2014-04-01 00:00:00

abstract：:Iminodiacetic acid (IDA) is one of the chelating ligands most frequently employed in immobilized metal-ion affinity chromatography (IMAC) due to its ability to act as electron-pair donor, forming stable complexes with intermediate and borderline Lewis metal ions (electron acceptor). Thus, IDA can also be employed in i...

journal_title：Applied biochemistry and biotechnology

authors： Bresolin ITL,Bresolin IRAP,Bueno SMA

更新日期：2020-06-01 00:00:00

abstract：:An improved procedure has been developed for efficient somatic embryogenesis in Anethum graveolens. Green friable embryogenic callus was obtained from hypocotyl segments on medium augmented with 2,4-dichlorophenoxyacetic acid (2,4-D). The highest embryogenic callus induction frequency of 87 % was obtained on Murashige...

journal_title：Applied biochemistry and biotechnology

authors： Dhir R,Shekhawat GS,Alam A

更新日期：2014-08-01 00:00:00

abstract：:Colour and COD removals of the azo dyes Congo Red (CR) and Reactive Black 5 (RB5) were individually evaluated in a sequential anaerobic/aerobic treatment system. Additionally, dye toxicity was assessed by using acute ecotoxicity tests with Daphnia magna as the indicator-organism. The anaerobic reactor was operated at ...

journal_title：Applied biochemistry and biotechnology

authors： Silva ME,Firmino PI,Sousa MR,Santos AB

更新日期：2012-02-01 00:00:00

abstract：:A biocompatible needle-type glucose sensor with a 3-electrode configuration was constructed. A platinum-electroplated carbon stick was used as the working electrode, Ag/AgCl as the reference electrode, and a disposable hypodermic needle made of stainless steel as the counter electrode. A Nafion membrane, an immobilize...

journal_title：Applied biochemistry and biotechnology

authors： Chen CY,Tamiya E,Ishihara K,Kosugi Y,Su YC,Nakabayashi N,Karube I

更新日期：1992-09-01 00:00:00

abstract：:In this study, we successfully performed Agrobacterium-mediated genetic transformation of Salvia miltiorrhiza and produced herbicide-resistant transformants. Leaf discs of S. miltiorrhiza were infected with Agrobacterium tumefaciens EHA105 harboring pCAMBIA 3301. The pCAMBIA 3301 includes an intron-containing gus repo...

journal_title：Applied biochemistry and biotechnology

authors： Liu Y,Yang SX,Cheng Y,Liu DQ,Zhang Y,Deng KJ,Zheng XL

更新日期：2015-12-01 00:00:00

abstract：:Catechol-2, 3-dioxygenase (C23O) from Pseudomonas sp. CGMCC2953 identified in our laboratory, which is one of the key enzymes responsible for phenanthrene biodegradation, was expected to get better characteristics tolerant to environment for its further application. With the aim of improving the enzyme properties by i...

journal_title：Applied biochemistry and biotechnology

authors： Wei J,Zhou Y,Xu T,Lu B

更新日期：2010-09-01 00:00:00

abstract：:The aim of this work is to investigate the binding affinity between the prokaryotic proteins-AHU-IHF proteins (AHU (AHU2, TR3, and AHU6) and IHF (IHF-WT and IHF-βE44A))-and DNAs (DNA, H'-DNA, and H'44A-DNA) by using the steered molecular dynamics (SMD) simulation and the molecular mechanics Poisson-Boltzmann surface a...

journal_title：Applied biochemistry and biotechnology

authors： Nguyen H,Pham T,Nguyen HL,Phan T

更新日期：2018-12-01 00:00:00

abstract：:Respiratory-deficient mutants of Rhizopus oryzae (R. oryzae) AS 3.3461 were acquired by ultraviolet (UV) irradiation to investigate changes in intracellular NADH metabolic pathway and its influence on the fermentation characteristics of the strain. Compared with R. oryzae AS 3.3461, the intracellular ATP level of the ...

journal_title：Applied biochemistry and biotechnology

authors： Shu C,Guo C,Luo S,Jiang S,Zheng Z

更新日期：2015-08-01 00:00:00