Abstract:
:The aim of this study was to determine whether transforming growth factor-beta1 (TGF-beta1) induces the synthesis, release and gene expression of urokinase-type plasminogen activator (uPA) in hepatic stellate cells. In addition to stimulating collagen production, TGF-beta1 induced the morphological and phenotypical changes characteristic of hepatic stellate cell activation. However, these changes accentuated in cells previously activated with acetaldehyde. TGF-beta1 increased to 2-fold uPA activity in lysates from quiescent cells, and to 3.5-fold in activated cells, and induced uPA gene expression to the same extent in both activated and non-activated cells. TGF-beta1 had a modest stimulatory action on the release of uPA into the conditioned medium, but reduced acetaldehyde-induced release, as demonstrated by Western blot analysis. In accord, whereas TGF-beta1 produces no effect on uPA activity in the conditioned media from quiescent cells, it significantly reduces the stimulatory action of acetaldehyde. These results show that the activity and gene expression of uPA are regulated by both acetaldehyde and TGF-beta1 and that the proteolytic activity in the extracellular space is reduced by the influence of TGF-beta1. Further studies on the molecular mechanisms responsible for the regulation of the plasminogen system by TGF-beta1 and other molecules in the presence of acetaldehyde will contribute to a better understanding of the processes involved in fibrogenesis.
journal_name
Pharmacologyjournal_title
Pharmacologyauthors
Pérez-Liz G,Flores-Hernández J,Arias-Montaño JA,Reyes-Esparza JA,Rodríguez-Fragoso Ldoi
10.1159/000081071subject
Has Abstractpub_date
2005-01-01 00:00:00pages
23-30issue
1eissn
0031-7012issn
1423-0313pii
81071journal_volume
73pub_type
杂志文章相关文献
PHARMACOLOGY文献大全abstract::Pain and mental status were assessed in a series of 35 consecutive hospitalized patients with metastatic cancer receiving narcotics for pain that was difficult to control. Forty-five episodes of mental status impairment were detected in 27 of these patients. Fifteen patients had dose-related oversedation or organic br...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000138294
更新日期:1987-01-01 00:00:00
abstract:AIMS:Recent studies have reported that intracellular calcium (Ca(2+)) mobilization is involved in homocysteine (Hcy)-induced endothelial dysfunction and the Na(+)/H(+) exchanger (NHE) is responsible for an increase in the intracellular Ca(2+) concentration in cardiovascular disease. We hypothesized that inhibition of t...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000356318
更新日期:2013-01-01 00:00:00
abstract::The effect of promethazine on the metabolic responsiveness of human polymorphonuclear leukocytes to opsonized zymosan was evaluated by chemiluminescence (CL) assay under the following situations: (1) preincubation of granulocytes with promethazine; (2) simultaneous addition of zymosan and promethazine, and (3) additio...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000136787
更新日期:1978-01-01 00:00:00
abstract::Angiotensin II (ANG II) is a potent vasoconstrictor in most vascular beds. We studied the role of cyclooxygenase products and/or endothelium-derived relaxing factor (EDRF) in modulating the pressor response to ANG II in the isolated, blood perfused dog lung. ANG II was given as a bolus dose of 2, 4 and 8 micrograms be...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000138709
更新日期:1990-01-01 00:00:00
abstract::Wood creosote has long been used as an antidiarrheal agent, but its mechanism of action is not well understood. To elucidate the mechanism of its antidiarrheal activity, we have addressed questions whether it inhibits fluid secretion induced by Escherichia coli heat-stable enterotoxin (STa) in rabbit jejunum in vivo, ...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000056092
更新日期:2001-01-01 00:00:00
abstract::The transketolase activity (TKA) of the white and red blood cells of oral contraceptive users was studied in an unselected sample population. Compared with healthy controls of corresponding age, the white cell TKA in the oral contraceptive groups was lowered in about 30%. The red cell TKA was lowered in about 15% of...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000136475
更新日期:1974-01-01 00:00:00
abstract::Mast cells from different locations are shown to vary in their histochemical, ultrastructural, cytochemical and functional properties. The clinical consequences of this heterogeneity and possible reasons for its origin are discussed. ...
journal_title:Pharmacology
pub_type: 杂志文章,评审
doi:10.1159/000138153
更新日期:1986-01-01 00:00:00
abstract:BACKGROUND/AIM:The effect of isocaloric diets and sibutramine on dietary behaviour and TNF-alpha is poorly understood. The aim of the study was to investigate the effects of isocaloric diets and sibutramine on food intake, body mass variation and serum TNF-alpha in free-feeding rats. METHODS:Three groups of male Wista...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000127363
更新日期:2008-01-01 00:00:00
abstract::The dependence of intestinal absorption of xenobiotics on the blood flow rate increases from blood flow independent to blood flow limited absorption as the absorbability of the substances increases. Since the absorbed substances are mainly drained by the blood flowing through the subepithelial vessels, not only the to...
journal_title:Pharmacology
pub_type: 杂志文章,评审
doi:10.1159/000137409
更新日期:1980-01-01 00:00:00
abstract:BACKGROUND/AIMS:Macitentan is a novel dual endothelin receptor antagonist with sustained receptor binding in clinical development for pulmonary arterial hypertension. The present study compared the pharmacokinetics and safety of macitentan in healthy Caucasian and Japanese subjects and explored the potential sex differ...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000351704
更新日期:2013-01-01 00:00:00
abstract::The antagonism of carbachol-induced contractions of guinea pig gallbladder smooth muscle strips via selective antagonists, methoctramine, HHSiD, pf-HHSiD and DABDMA has been investigated in order to find out the m-cholinoceptor subtype(s) of gallbladder smooth muscle. All m-cholinoceptor antagonists examined, displace...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000139061
更新日期:1993-06-01 00:00:00
abstract::The absorption into the systemic circulation of compounds administered intraperitoneally in large volumes was investigated in the rat. The influence on absorption of molecular weight, lipid-water partition coefficient (K), and dissociation constant (pKa) was studied. Nine neutral compounds ranging in molecular weight ...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000136874
更新日期:1978-01-01 00:00:00
abstract:BACKGROUND/AIM:It has been demonstrated that dexamethasone-induced hypertension can be prevented by the NADPH oxidase inhibitor apocynin. The effect of dexamethasone on NADPH oxidase, however, is unknown. The present study was conducted to investigate the effect of dexamethasone on the gene expression of Nox1, the majo...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000365932
更新日期:2014-01-01 00:00:00
abstract::Anoxia has been shown to potentiate the constrictor effects of 5-hydroxytryptamine (5HT) in isolated vascular tissue. In the present study, canine coronary arterial rings were incubated with various treatments and exposed to 5HT (4 X 10(-7) M) and anoxia (95% N2 and 5% CO2). Developed tension was increased by 250 +/- ...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000138211
更新日期:1986-01-01 00:00:00
abstract::The affinity of amitriptyline for muscarine M1 receptors was studied in the rat cerebral cortex and rabbit vas deferens utilizing binding studies as well as inhibition of carbachol-induced phosphoinositide hydrolysis in the cerebral cortex and blockade of the muscarinic prejunctional inhibition of sympathetic nerve st...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000139192
更新日期:1994-05-01 00:00:00
abstract::High-affinity (104 +/- 18 pmol/l) and high-density (204 +/- 25 fmol/mg) angiotensin II (AII) binding sites have been identified in Xenopus laevis heart membranes. Competition binding of [125I]Sar1,Ile8 angiotensin (SIA) to these receptors by peptide analogs selective for the mammalian AII receptor subtypes AT1 and AT2...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000139186
更新日期:1994-04-01 00:00:00
abstract::The hypoxia-inducible factor 1alpha (HIF-1alpha), a member of the PAS superfamily, is a global regulator of cellular and systemic O(2) homeostasis as well as embryonic development. As the activity of HIF-1alpha is increased by a lowered oxygen tension in vivo and in vitro, we established a cell line producing high amo...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000072359
更新日期:2003-10-01 00:00:00
abstract::The insulinogenic response to a standard i.v. dose of a sulfonylurea can be markedly augmented in normal, conscious dogs if they are given 30 min earlier a single i.v. dose of dihydroergotamine (DHE). Since the parent substance ergotamine posssed no such amplifying properties, further experiments were conducted to cla...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000136697
更新日期:1977-01-01 00:00:00
abstract::We investigated the effects of calcium ions (Ca2+) on the adenylyl cyclase activity in purified turkey erythrocyte membranes. Results showed the following: (i) Ca2+ inhibits cAMP accumulation stimulated by isoproterenol (1 micromol/l), NaF + AlCl3 (10 mmol/l + 20 micromol/l) or forskolin (10 micromol/l) in EGTA-washed...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000028245
更新日期:1998-10-01 00:00:00
abstract::We established an Ascaris-induced biphasic skin allergic reaction in mice. In the early-phase reaction (EPR), mast cell degranulation was observed, and tranilast inhibited ear edema. In mast-cell-deficient mice (WBB6F(1)-W/W(V) mice), ear edema in the EPR disappeared, whereas that in the late-phase reaction (LPR) rema...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000056117
更新日期:2001-01-01 00:00:00
abstract::In the present paper, two experiments are performed to test the efficacy of the intravenous administration of hypertonic glucose (25 or 50%) in alcoholic intoxication. In a first experiment, 10 healthy, nonstarved volunteers, received 15 min after the ingestion of 1.0 g/kg alcohol, 40 ml of 25% glucose i.v., the same ...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000137769
更新日期:1983-01-01 00:00:00
abstract::Chronic exposure to cold increases the growth of brown adipose tissue and the resistance to more severe cold, thus improving thermogenesis. The present study examined the possibility that dietary compounds can modify cold acclimation. Adenosine (ADO) or adenine (ADE) were administered in drinking water (0.05%, w/v) fo...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000028315
更新日期:1999-09-01 00:00:00
abstract::Erythrocytes can take up amino acids from the blood by using a variety of transport systems. GLYT is a key transport protein in the plasma membrane responsible for the Na(2+)-dependent uptake of glycine needed for glutathione biosynthesis. Certain cysteine-specific compounds, particularly mercuric chloride and 5,5'-di...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000074973
更新日期:2004-03-01 00:00:00
abstract:BACKGROUND/AIMS:The neurotransmitters participating in the nerve-mediated contraction of the guinea pig seminal vesicle (GPSV) have not been firmly established. There is debate as to the mediating role of norepinephrine and acetylcholine. METHODS:We have used longitudinally and circularly oriented strips of GPSV and a...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000351847
更新日期:2013-01-01 00:00:00
abstract::In a randomized three-phase crossover study, 12 healthy male volunteers were given three 12-hourly 500-mg doses of erythromycin base, as enteric coated pellets in capsules (2 X 250 mg), erythromycin stearate tablet (1 X 500 mg), or erythromycin ethylsuccinate sachet (1 X 500 mg). The reaction time after administration...
journal_title:Pharmacology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1159/000138029
更新日期:1984-01-01 00:00:00
abstract::Zopiclone (ZPC; RP 27,267), which is chemically unrelated to benzodiazepines (BZD), was found to have a similar pharmacological profile and to possess in man hypnotic activity similar to that of some BZD such as nitrazepam. It was, therefore, interesting to study the interaction of ZPC with rat brain receptors and spe...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000137912
更新日期:1983-01-01 00:00:00
abstract::Renal hypoperfusion which occurs in hemorrhagic shock creates an environment in which cellular injury and organ dysfunction can occur during the episode of shock as well as reoxygenation and reperfusion. At the same time, mast cell degranulation which is observed during hemorrhage may have an additional deleterious ef...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000139097
更新日期:1993-09-01 00:00:00
abstract:BACKGROUND/AIMS:morphine is known to inhibit cholinergic contractions of the guinea pig small intestine. This has been compared to the human small intestinal innervated longitudinal muscle in the current study. METHODS:cholinergic primary contractions of human small intestinal longitudinal strips were evoked by electr...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000316637
更新日期:2010-01-01 00:00:00
abstract:BACKGROUND/AIMS:Flaxseed contains alpha-linolenic acid (ALA), lignans, and dietary fiber, and its intake lowers blood pressure in hypertensive patients. Here, we examined the effects of flaxseed powder, which includes all flaxseed components, flaxseed oil, composed mainly of ALA, flaxseed lignan, and flaxseed fiber, on...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000502789
更新日期:2020-01-01 00:00:00
abstract::When rat mast cells were cultured for a short period in plastic dishes and adhering cells were permeabilized with beta-escin and exposed to Ca2+ at concentrations higher than 10(-7) mol/l, histamine release was induced dose-dependently. Protein kinase C (PKC) activity in the crude extracts obtained from adhering mast ...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000138874
更新日期:1992-01-01 00:00:00