Modulation of urokinase-type plasminogen activator by transforming growth factor beta1 in acetaldehyde-activated hepatic stellate cells.


:The aim of this study was to determine whether transforming growth factor-beta1 (TGF-beta1) induces the synthesis, release and gene expression of urokinase-type plasminogen activator (uPA) in hepatic stellate cells. In addition to stimulating collagen production, TGF-beta1 induced the morphological and phenotypical changes characteristic of hepatic stellate cell activation. However, these changes accentuated in cells previously activated with acetaldehyde. TGF-beta1 increased to 2-fold uPA activity in lysates from quiescent cells, and to 3.5-fold in activated cells, and induced uPA gene expression to the same extent in both activated and non-activated cells. TGF-beta1 had a modest stimulatory action on the release of uPA into the conditioned medium, but reduced acetaldehyde-induced release, as demonstrated by Western blot analysis. In accord, whereas TGF-beta1 produces no effect on uPA activity in the conditioned media from quiescent cells, it significantly reduces the stimulatory action of acetaldehyde. These results show that the activity and gene expression of uPA are regulated by both acetaldehyde and TGF-beta1 and that the proteolytic activity in the extracellular space is reduced by the influence of TGF-beta1. Further studies on the molecular mechanisms responsible for the regulation of the plasminogen system by TGF-beta1 and other molecules in the presence of acetaldehyde will contribute to a better understanding of the processes involved in fibrogenesis.






Pérez-Liz G,Flores-Hernández J,Arias-Montaño JA,Reyes-Esparza JA,Rodríguez-Fragoso L




Has Abstract


2005-01-01 00:00:00














  • Inhibition of prostaglandin biosynthesis in human endotoxin-stimulated peripheral blood monocytes: effects of caffeine.

    abstract::There is an ongoing debate about possible advantages of the coadministration of caffeine with cyclooxygenase (COX) inhibitors in the treatment of pain. There are results suggesting interference by caffeine with COX expression and activity in rat immune cells. In the present study, we have used, therefore, human endoto...


    pub_type: 杂志文章


    authors: Ulcar R,Schuligoi R,Heinemann A,Santner B,Amann R

    更新日期:2003-02-01 00:00:00

  • Pharmacokinetics of two novel bicalutamide formulations in healthy male volunteers.

    abstract::The oral bioavailability of two investigational formulations of bicalutamide was compared with the current clinical formulation. The first formulation was amorphous R-/S-bicalutamide in solid dispersion with a polymeric matrix of hydroxypropyl methylcellulose phthalate (HP55S) (R-/S-bicalutamide/HP55S); the second was...


    pub_type: 杂志文章,随机对照试验


    authors: Cantarini M,Fuhr R,Morris T

    更新日期:2006-01-01 00:00:00

  • Differential contractile impairment of fast- and slow-twitch skeletal muscles in a rat model of doxorubicin-induced congestive heart failure.

    abstract::Congestive heart failure (CHF) is associated with exercise intolerance that cannot be entirely explained by hypoperfusion of the skeletal muscles. We studied the contractile properties of fast-twitch (extensor digitorum longus; EDL) and slow-twitch (soleus; SOL) skeletal muscles in doxorubicin-induced CHF in rats, and...


    pub_type: 杂志文章


    authors: Ertunc M,Sara Y,Korkusuz P,Onur R

    更新日期:2009-01-01 00:00:00

  • Effects of fructose-1,6-diphosphate on the activity of rat liver nitric oxide synthase in vitro.

    abstract::Fructose-1,6-diphosphate (FDP) was found to cause significant stimulation of nitric oxide synthase (NOS) in rat liver homogenates in vitro. This effect was more pronounced for the inducible isoform than its constitutive counterpart. Furthermore, FDP restored rat liver inducible NOS levels following their depletion by ...


    pub_type: 杂志文章


    authors: Mihas AA,Maliakkal RJ,Mihas TA,Kanji VK,Markov AK

    更新日期:1997-01-01 00:00:00

  • Delta-sleep-inducing peptide potentiates anticonvulsive activity of valproate against metaphit-provoked audiogenic seizure in rats.

    abstract::The effect of delta-sleep-inducing peptide (DSIP) on the anticonvulsive activity of a nonprotective valproate (VPA) dose in a metaphit model of generalized, reflex audiogenic seizures in adult Wistar rats was studied. The animals that received metaphit (10 mg/kg) were exposed to audiogenic stimulation (100 +/- 3 dB, 6...


    pub_type: 杂志文章


    authors: Hrncić D,Stanojlović O,Zivanović D,Susić V

    更新日期:2006-01-01 00:00:00

  • Is [3H]-tiotidine a specific ligand for the H2-receptor?

    abstract::The H2-antagonist tiotidine inhibited the H2-receptor-mediated, histamine-induced increase in cyclic AMP in dispersed mucosal cells from guinea pig stomach (Ki, 4 X 10(-8) M). The radiolabeled [3H]-tiotidine bound specifically and reversibly to the same cells with a half-maximal binding occurring at 5 X 10(-7) M tioti...


    pub_type: 杂志文章


    authors: Batzri S,Harmon JW

    更新日期:1986-01-01 00:00:00

  • On the heterogeneity of mast cells.

    abstract::Mast cells from different locations are shown to vary in their histochemical, ultrastructural, cytochemical and functional properties. The clinical consequences of this heterogeneity and possible reasons for its origin are discussed. ...


    pub_type: 杂志文章,评审


    authors: Pearce FL

    更新日期:1986-01-01 00:00:00

  • Mechanism of action of the anti-shock effect of CCK-8: influence of CCK antagonists and of sympatholytic drugs.

    abstract::In an experimental model of haemorrhagic shock that causes 100% mortality in rats within 30 min, the intravenous bolus injection (20 micrograms/kg) of sulfated cholecystokinin octapeptide (CCK-8) induces a prompt and sustained rise in blood pressure and pulse amplitude, all treated animals still surviving at the end o...


    pub_type: 杂志文章


    authors: Guarini S,Vergoni AV,Bertolini A

    更新日期:1988-01-01 00:00:00

  • Lithium absorption, distribution and clearance and body temperature in rats given lithium plus haloperidol.

    abstract::Renal lithium clearance, tissue lithium levels, serum lithium concentration, and body temperature were determined in rats given lithium alone or combined with haloperidol (0.5-1 mg/kg). A slight increase in serum lithium concentration occurred in one of the three groups given lithium plus haloperidol. Haloperidol fail...


    pub_type: 杂志文章


    authors: Smith DF,Shimizu M,Schou M

    更新日期:1977-01-01 00:00:00

  • Studies on the mechanism of action of colloidal bismuth subcitrate. I. Interaction with sulfhydryls.

    abstract::The present study was designed to examine the reaction pathway of colloidal bismuth subcitrate (CBS) with thiols. Studies were performed using the monothiol glutathione (GSH), the dithiol dithiothreitol (DTT) and the thiol enzymes papain and H+/K(+)-ATPase. UV-vis spectra showed that CBS forms complexes with GSH and D...


    pub_type: 杂志文章


    authors: Beil W,Bierbaum S,Sewing KF

    更新日期:1993-08-01 00:00:00

  • Tissue distribution of primaquine in the rat.

    abstract::The distribution of primaquine was measured in seven rat tissues at 15-180 min after the intraperitoneal injection of the antimalarial 8-aminoquinoline. The half-life of unmetabolized primaquine was 4.0 h in lung, 1.7-1.9 h in blood, spleen, kidney and heart, and 1.2 h in liver. At each interval, the concentrations of...


    pub_type: 杂志文章


    authors: Holbrook DJ Jr,Griffin JB,Fowler L,Gibson BR

    更新日期:1981-01-01 00:00:00

  • Effects of triamterene and its phase I and phase II metabolities on sodium transport of the isolated frog skin.

    abstract::We studied the effects of triamterene (TA) on its phase I and phase II metabolites, p-hydroxytriamterene (OH-TA) and p-hydroxytriamterene sulfuric acid ester (OH-TA ester), on sodium transport in the isolated frog skin. While TA applied to the inside (corial side) surface had no effects on potential difference (PD) an...


    pub_type: 杂志文章


    authors: Kramer HJ,Rörig M,Völger KD

    更新日期:1981-01-01 00:00:00

  • Study of the vasodilating activity of salbutamol on dog coronary arteries. Unexpected effects of methylene blue.

    abstract::The vascular relaxant effect of salbutamol and its dependence on the endothelium were studied in the isolated dog coronary artery, precontracted with prostaglandin F2 alpha. Salbutamol induced a concentration-dependent relaxation which was partially inhibited by removal of endothelial cells. Atenolol 10(-6) mol/l, a b...


    pub_type: 杂志文章


    authors: Bernard F,Jouquey S,Hamon G

    更新日期:1991-01-01 00:00:00

  • Histamine H(3) receptors are involved in the protective effect of ghrelin against HCl-induced gastric damage in rats.

    abstract::In the present study, the effects of ghrelin against the gastric damage induced by intragastric administration of 0.6 N HCl and the involvement of histamine H₃ receptors (H₃Rs) were investigated in conscious rats with selective H₃R ligands. Intraperitoneal (i.p.) injection of ghrelin (40 μg/kg) significantly reduced (...


    pub_type: 杂志文章


    authors: Adami M,Pozzoli C,Leurs R,Stark H,Coruzzi G

    更新日期:2010-01-01 00:00:00

  • On the cardiac contractile, electrophysiological and biochemical effects of endothall, a protein phosphatase inhibitor.

    abstract::Protein phosphatase inhibitors, e.g. cantharidin, exert positive inotropic effects in mammalian heart preparations. Endothall, a synthetic herbicide which is chemically related to cantharidin, inhibits protein phosphatase activities in mouse liver preparations. However, the cardiac effects of endothall have hitherto n...


    pub_type: 杂志文章


    authors: Bokník P,Vahlensieck U,Huke S,Knapp J,Linck B,Lüss H,Müller FU,Neumann J,Schmitz W

    更新日期:2000-07-01 00:00:00

  • Efficacy and safety of two analogs of L-carnitine on rats made insulin resistant by a high-fructose diet.

    abstract:AIM:To evaluate the efficacy and safety of 2 analogs of L-carnitine on rats made insulin resistant by a high-fructose diet. METHODS:Using rats made insulin resistant by a high-fructose diet, we investigated the impact of 2 analogs of L-carnitine (25 mg/kg) and L-carnitine (250 mg/kg) on glucose, triglycerides and chol...


    pub_type: 杂志文章


    authors: Gómez-Solís A,De la Cruz-Cordero R,Avalos-Soriano A,Duarte-Vázquez MA,Reyes-Esparza J,Rodríguez-Fragoso L

    更新日期:2011-01-01 00:00:00

  • In vitro effects of sennoside on contractile activity and fluid flow in the perfused large intestine of the rat.

    abstract::The effect of sodium rhein on contractile activity and fluid flow in the rat complete large intestine was studied in vitro. Contractile activity was recorded using serosal strain gauges and volume transducers recorded distal fluid flow from the segment. Luminal sodium rhein (1 mM) produced a protracted increase in cae...


    pub_type: 杂志文章


    authors: Rumsey RD,Squires PE,Read NW

    更新日期:1993-10-01 00:00:00

  • Effects of cyclic adenosine monophosphate-dependent protein kinase and calcium-dependent protein kinase modulators on stimulated gastric acid secretion in the perfused rat stomach.

    abstract::The effects of cyclic adenosine monophosphate-dependent protein kinase (PKA) and calcium-dependent protein kinase (PKC) modulators on secretagogue-stimulated gastric acid secretion were studied in the continuously perfused stomach of the anesthetized rat. Intravenous histamine (0.25 mg/kg/h) and pentagastrin (2 microg...


    pub_type: 杂志文章


    authors: Rotker JD,Strapko A,Nandi J,Bosche MC,Levine RA

    更新日期:1995-10-01 00:00:00

  • Effect of YM-44781, YM-44778 and YM-49598, novel tachykinin antagonists, in a drug-induced bladder contraction model.

    abstract::The radioligand binding profiles and in vivo pharmacological characteristics of YM-44781, YM-44778 and YM-49598, novel non-peptide tachykinin receptor antagonists, were examined and compared to those of FK-888 and GR-159897. Since no functional NK(3) receptors were found in the rat bladder, the emphasis will be on the...


    pub_type: 杂志文章


    authors: Choppin A,Groke G,Bringas A,Stepan G,Dillon MP

    更新日期:2002-05-01 00:00:00

  • Effects of spasmogens on relaxant dose-response curves to full or partial agonists at beta-adrenoceptors of guinea pig isolated trachea.

    abstract::When guinea pig isolated trachea is contracted with either carbachol or methacholine, relaxant concentration-response curves to isoprenaline, salmefamol and fenoterol were shifted to the right, compared with curves obtained with either spontaneous or histamine-induced tone. Carbachol and methacholine also reduced the ...


    pub_type: 杂志文章


    authors: Hutson PA,Carpenter JR

    更新日期:1987-01-01 00:00:00

  • Evaluation of pharmacological profile of meloxicam as an anti-inflammatory agent, with particular reference to its relative selectivity for cyclooxygenase-2 over cyclooxygenase-1.

    abstract::We studied the anti-inflammatory activity of meloxicam on rat carrageenin-induced pleurisy and its toxicity for rat gastric mucosa, relative to its in vitro inhibitory potency against partially purified cyclooxygenase (COX)-1 and COX-2 preparations in order to clarify the pharmacological profile of the compound as an ...


    pub_type: 杂志文章


    authors: Ogino K,Hatanaka K,Kawamura M,Katori M,Harada Y

    更新日期:1997-07-01 00:00:00

  • Stereoselective glucuronidation of carvedilol in human liver and intestinal microsomes.

    abstract:BACKGROUND AND PURPOSE:Carvedilol is used clinically as a β-adrenoceptor antagonist for the treatment of chronic heart failure and is primarily metabolized into glucuronides by UDP-glucuronosyltransferase (UGT). In this study, the stereoselective glucuronidation of carvedilol by the human liver and intestinal microsome...


    pub_type: 杂志文章


    authors: Hanioka N,Tanaka S,Moriguchi Y,Narimatsu S

    更新日期:2012-01-01 00:00:00

  • Time course of the distribution of morphine in brain regions, spinal cord and serum following intravenous injection to rats of differing ages.

    abstract::Previously it was demonstrated that intravenously administered morphine produced greater analgesic but lower hyperthermic responses to morphine in 24-week-old rats in comparison to 8-week-old rats. The differential pharmacological responses to morphine could not solely be attributed to the pharmacokinetic parameters, ...


    pub_type: 杂志文章


    authors: Bhargava HN,Villar VM,Rahmani NH,Larsen AK

    更新日期:1993-07-01 00:00:00

  • Stimulation of microsomal spironolactone metabolism by reduced glutathione.

    abstract::The first step in the conversion of spironolactone (SP) to its biologically active metabolites is deacetylation to 7 alpha-thiospirolactone (7 alpha-thio-SL). Studies were done to evaluate the effects of reduced glutathione (GSH) on SP deacetylation by adrenal microsomal preparations. In the absence of GSH, adrenal mi...


    pub_type: 杂志文章


    authors: Colby HD,LaCagnin LB,Los LE

    更新日期:1996-01-01 00:00:00

  • Inhibitory effects of J78, a newly synthesized 1,4-naphthoquinone derivative, on experimental thrombosis and platelet aggregation.

    abstract::Several compounds with the backbone of 1,4-naphthoquinone chemical structure have been reported to display antiplatelet and antithrombotic activities, indicating that this congener compound may be a new source in the antithrombotic drug development. In the present study, the possible antiplatelet activity and antithro...


    pub_type: 杂志文章


    authors: Jin YR,Ryu CK,Moon CK,Cho MR,Yun YP

    更新日期:2004-04-01 00:00:00

  • Positive inotropic action of veratridine in rat atria: possible involvement of prostanoids.

    abstract::Veratridine caused a positive inotropic action in the electrically driven left atria of rats. Quinacrine (a phospholipase A2 inhibitor), indomethacin (a cyclooxygenase inhibitor) and aspirin (a cyclooxygenase inhibitor), but not nordihydroguaiaretic acid (a lipoxygenase inhibitor), inhibited the response to veratridin...


    pub_type: 杂志文章


    authors: Morikawa M,Satake N,Shibata S,Shibata T

    更新日期:1991-01-01 00:00:00

  • Effects of 2(3)-tert-butyl-4-hydroxyanisole pretreatment on cefpiramide binding to mouse glutathione S-transferases.

    abstract::Binding of cefpiramide (CPM) and other beta-lactam antimicrobial agents to 2(3)-tert-butyl-4-hydroxyanisole (BHA)-induced liver glutathione (GSH) S-transferases (EC from CD-1 mice was studied. A marked induction of hepatic GSH S-transferase from mice fed BHA was observed. Gel chromatography of liver cytosol ...


    pub_type: 杂志文章


    authors: Nishiya H,Haga T,Nozue N,Komatsu T,Baba M,Ueda Y,Ono Y,Kunii O

    更新日期:1989-01-01 00:00:00

  • Effects of magnesium on human umbilical arteries: role of intracellular calcium stores.

    abstract::The purpose of this investigation on human umbilical arteries was: (1) to analyze the effects of magnesium (Mg2+) in the prevention of contractions induced by 5-hydroxytryptamine (5-HT), KCl, Bay K 8644 and BaCl2; (2) to determine whether the presence of Mg2+ could modulate the response to caffeine in Ca2+-free medium...


    pub_type: 杂志文章


    authors: Tiritilli A

    更新日期:1998-12-01 00:00:00

  • Sennosides and aloin do not promote dimethylhydrazine-induced colorectal tumors in mice.

    abstract::In a model of dimethylhydrazine-induced colorectal tumors in male mice aloin- or sennoside-enriched diets (0.03%) did not promote incidence and growth of adenomas and carcinomas after 20 weeks. Furthermore, in anthranoid-fed mice no significant changes in serum electrolytes as well as parameters of hepato- and nephrot...


    pub_type: 杂志文章


    authors: Siegers CP,Siemers J,Baretton G

    更新日期:1993-10-01 00:00:00

  • Clofibrate decreases jejunal cholesterol synthesis and activity of postheparin plasma lipoprotein lipase in the rat.

    abstract::Clofibrate treatment decreased drastically the activity of serum postheparin lipoprotein lipase of rats fed with fat-free test diet but had no effect on hepatic lipase. 3 weeks' treatment was followed by a significant fall in serum cholesterol caused by a marked decrease in HDL-cholesterol. Clofibrate decreased in vit...


    pub_type: 杂志文章


    authors: Strandberg TE,Kuusi T,Tilvis RS,Miettinen TA

    更新日期:1983-01-01 00:00:00