Abstract:
:Tracheal aspirate IL-8 concentration and airway epithelial cell IL-8 expression are each increased in premature infants undergoing mechanical ventilation. We sought to determine the cytokines responsible for IL-8 expression in this context. Tracheal aspirates were collected from 18 mechanically ventilated premature infants. IL-8 protein abundance was high in tracheal aspirates from ventilated premature infants (mean, 5806 +/- 4923 pg/mL). IL-1 alpha (mean, 20 +/- 6 pg/mL), IL-1 beta (mean 67 +/- 46 pg/mL), and tumor necrosis factor (TNF)-alpha (mean, 8 +/- 2 pg/mL) were also found. Incubation of tracheal aspirates with 16HBE14o- human bronchial epithelial cells increased IL-8 protein in both cell lysates and supernatants, as well as transcription from the IL-8 promoter. Aspirates also induced nuclear factor (NF)-kappa B activation. Mutation of the IL-8 promoter NF-kappa B site abolished aspirate-induced IL-8 transcription. Endotoxin concentrations in the tracheal aspirates were negligible and incapable of inducing IL-8 promoter activity. Finally, incubation of tracheal aspirates with a neutralizing antibody against IL-1 beta reduced epithelial cell IL-8 production, whereas neutralizing antibodies against IL-1 alpha and TNF-alpha had no effect. We conclude that airway fluid from mechanically ventilated premature infants contains soluble factors capable of inducing airway epithelial cell IL-8 expression via a NF-kappa B-dependent pathway, and that IL-1 beta plays a specific role in this process.
journal_name
Pediatr Resjournal_title
Pediatric researchauthors
Shimotake TK,Izhar FM,Rumilla K,Li J,Tan A,Page K,Brasier AR,Schreiber MD,Hershenson MBdoi
10.1203/01.PDR.0000145274.47221.10subject
Has Abstractpub_date
2004-12-01 00:00:00pages
907-13issue
6eissn
0031-3998issn
1530-0447pii
01.PDR.0000145274.47221.10journal_volume
56pub_type
杂志文章abstract::The developmental stages of each foregut organ are intimately linked to the development of the other foregut organs such that the ultimate function of any one foregut organ, such as the metabolic function of the liver, depends on organizational changes associated with the maturation of multiple foregut organs. These c...
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pub_type: 杂志文章,评审
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pub_type: 杂志文章,meta分析
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更新日期:2000-09-01 00:00:00
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更新日期:2015-08-01 00:00:00
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更新日期:1976-07-01 00:00:00
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更新日期:1988-01-01 00:00:00
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更新日期:2006-06-01 00:00:00
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