当前位置： SCI文献检索 > PEDIATRIC RESEARCH期刊下所有文献 > Intrauterine infection induces programmed cell death in rabbit periventricular white matter.

Intrauterine infection induces programmed cell death in rabbit periventricular white matter.

Abstract：

:An association between chorioamnionitis and periventricular leukomalacia has been reported in human preterm infants. However, whether this link is causal has not been convincingly established, and the underlying molecular mechanisms remain unclear. The objective of this study was to establish a reproducible model of cerebral white matter disease in preterm rabbits after intrauterine infection. Escherichia coli was inoculated into both uterine horns of laparotomized pregnant rabbits when gestation was 80% complete. The fetuses were delivered by cesarean section and killed 12, 24, or 48 h after the inoculation. Programmed cell death in the white matter was evaluated by hematoxylin-eosin-saffron staining and in situ fragmented DNA labeling (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling). In a first group of 14 pregnant rabbits not treated with antibiotics, all fetuses delivered 48 h after inoculation were stillborn, whereas fetuses extracted 12 or 24 h after inoculation were alive. No significant cell death was detected in the live fetuses compared with the control noninfected rabbits. In a second group of five pregnant rabbits treated with ceftriaxone initiated 24 h after the inoculation and continued until cesarean section was performed 48 h after inoculation, 13 fetuses were alive, but all showed evidence of extensive programmed cell death in the white matter by hematoxylin-eosin-saffron staining and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling. White matter damage became histologically detectable only 48 h after inoculation. Three of the 13 brains displayed periventricular white matter cysts mimicking human cystic periventricular leukomalacia. The high reproducibility of white matter damage in our model should permit further studies aimed at unraveling the molecular mechanisms of periventricular leukomalacia.

journal_name

Pediatr Res

journal_title

Pediatric research

authors

Debillon T,Gras-Leguen C,Vérielle V,Winer N,Caillon J,Rozé JC,Gressens P

doi

10.1203/00006450-200006000-00009

subject

Has Abstract

pub_date

2000-06-01 00:00:00

pages

736-42

issue

eissn

0031-3998

issn

1530-0447

journal_volume

pub_type

杂志文章

在线工具

Intrauterine infection induces programmed cell death in rabbit periventricular white matter.