Interleukin-10 inhibits proinflammatory chemokine release by neutrophils of the newborn without suppression of nuclear factor-kappa B.


:An increase in polymorphonuclear leukocytes (PMNs) and proinflammatory chemokines, such as IL-8 and macrophage inflammatory protein-1 alpha (MIP), are found in the airways during early stages of bronchopulmonary dysplasia. We determined whether IL-10 produces a dose-related inhibition of proinflammatory chemokine release from stimulated neutrophils of the newborn and whether the mechanism involves the pivotal transcription factor, nuclear factor-kappa B. PMNs isolated from the cord blood of healthy newborns were stimulated submaximally with either lipopolysaccharide (n = 5) or tumor necrosis factor (n = 4), with and without IL-10 (0.01-1000 ng/mL). IL-8 and MIP release were measured in cell culture supernatants at 18 h. The presence or absence of nuclear factor-kappa B activity and inhibitor-kappa B alpha degradation was measured at 30 min and 3 h after PMN stimulation began. During lipopolysaccharide stimulation, IL-10 significantly reduced IL-8 levels from 50 +/- 16 ng/mL to 7 +/- 3 ng/mL, and MIP levels from 14 +/- 5 to 0.7 +/- 0.1 ng/mL (mean +/- SEM, p < 0.01). IL-10 produced an insignificant reduction in IL-8 and MIP levels after stimulation of PMNs with tumor necrosis factor. IL-10 did not inhibit nuclear factor-kappa B activation and inhibitor-kappa B alpha degradation in PMNs stimulated with tumor necrosis factor or lipopolysaccharide for 30 min. After PMN stimulation for 3 h, inhibitor-kappa B alpha cytoplasmic levels were restored; however, they were unaffected by IL-10. We conclude that IL-10 is a potent inhibitor of lipopolysaccharide-stimulated release of IL-8 and MIP from neutrophils of the newborn via a mechanism not involving nuclear factor-kappa B activity. Further work is needed to determine whether exogenous IL-10 may be useful for suppressing inflammation in bronchopulmonary dysplasia.


Pediatr Res


Pediatric research


Tryzmel J,Miskolci V,Castro-Alcaraz S,Vancurova I,Davidson D




Has Abstract


2003-09-01 00:00:00














  • Effect of temperature on thromboelastography and implications for clinical use in newborns undergoing therapeutic hypothermia.

    abstract:BACKGROUND:Encephalopathic neonates undergoing therapeutic hypothermia have increased risk for coagulopathy secondary to perinatal asphyxia and effects of cooling on the coagulation enzyme cascade. Thromboelastography (TEG) allows for a comprehensive assessment of coagulation that can be regulated for temperature. TEG ...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Forman KR,Wong E,Gallagher M,McCarter R,Luban NL,Massaro AN

    更新日期:2014-05-01 00:00:00

  • Heart rate dynamics in low risk human fetuses.

    abstract::Evaluation of nonlinear heart rate (HR) dynamics has received considerable attention in the pediatric literature because such analyses not only provide insight into underlying control mechanisms, but may also help to differentiate between normal and abnormal infants. The purpose of this study was to determine, in eigh...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Mooney DM,Groome LJ,Holland SB,Smith YD

    更新日期:1998-07-01 00:00:00

  • Defective leukocyte fungicidal activity in end-organ resistance to 1,25-dihydroxyvitamin D.

    abstract::Recent studies have shown 1,25(OH)2D3 receptor-mediated modulation of leukocyte proliferation, differentiation, and function. We examined the phagocytosis and killing of microorganisms by neutrophils and monocytes from five patients of three families with hereditary resistance to 1,25(OH)2D3. Phagocytosis of microorga...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Etzioni A,Hochberg Z,Pollak S,Meshulam T,Zakut V,Tzehoval E,Keisari Y,Aviram I,Spirer Z,Benderly A

    更新日期:1989-03-01 00:00:00

  • Prostaglandin E2 attenuates hyperoxia-induced injury in cultured rabbit tracheal epithelial cells.

    abstract::We assessed the kinetics of hyperoxia-induced prostaglandin E2 (PGE2) production by cultured rabbit tracheal epithelial (TE) cells with different inherent capacities to generate PGE2 and the role of endogenous PGE2 production in protecting these cells from hyperoxic injury. Rabbit TE cells grown to confluence with or ...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Dennery PA,Walenga RW,Kramer CM,Alpert SE

    更新日期:1992-07-01 00:00:00

  • Clinical and biochemical findings before and after portacaval shunt in a girl with type Ib glycogen storage disease.

    abstract::A girl presented with an important growth retardation, hepatomegaly, fasting hypoglycemia, lactic acidosis, increased serum cholesterol, triglycerides and uric acid, and increased liver glycogen (7.5%). There was no rise in blood glucose after IV galactose or fructose, but glucagon gave a delayed response. Type Ib gly...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Corbeel L,Hue L,Lederer B,De Barsy T,Van den Berghe G,Devlieger H,Jaeken J,Bracke P,Eeckels R

    更新日期:1981-01-01 00:00:00

  • Delineation of late onset hypoventilation associated with hypothalamic dysfunction syndrome.

    abstract::Late Onset Central Hypoventilation Syndrome associated with Hypothalamic Dysfunction (LO-CHS/HD) is a distinct entity among the clinical and genetic heterogeneous group of patients with late onset central hypoventilation. Here we report a series of 13 patients with LO-CHS/HD. Rapid onset obesity is the first symptom o...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: De Pontual L,Trochet D,Caillat-Zucman S,Abou Shenab OA,Bougneres P,Crow Y,Cunningham S,Esteva B,Heberle LC,Leger J,Pinto G,Polak M,Shafik MH,Straus C,Trang H,Munnich A,Lyonnet S,Desguerre I,Amiel J

    更新日期:2008-12-01 00:00:00

  • Urologic tissue engineering in pediatrics: from nanostructures to bladders.

    abstract::The use of tissue engineering to develop new urinary bladder could have direct impact on children suffering from congenital bladder dysfunction. Current bladder tissue engineering protocols rely on scaffolds to support the ingrowth of new cells and the subsequent development of new tissues. The interaction between cel...

    journal_title:Pediatric research

    pub_type: 杂志文章,评审


    authors: Roth CC

    更新日期:2010-05-01 00:00:00

  • Diagnostics for neonatal sepsis: current approaches and future directions.

    abstract::Progress has been made in the reduction of morbidity and mortality from neonatal sepsis. However, diagnosis continues to rely primarily on conventional microbiologic techniques, which can be inaccurate. The objective of this review is to provide the clinician with an overview of the current information available on di...

    journal_title:Pediatric research

    pub_type: 杂志文章,评审


    authors: Iroh Tam PY,Bendel CM

    更新日期:2017-10-01 00:00:00

  • Mouse model of mevalonate kinase deficiency: comparison of cytokine and chemokine profile with that of human patients.

    abstract:BACKGROUND:Mevalonate kinase deficiency (MKD) is a rare genetic autoinflammatory disease caused by blocking of the enzyme mevalonate kinase in the pathway of cholesterol and isoprenoids. The pathogenic mechanism originating an immune response in MKD patients has not been clearly understood. METHODS:We investigated the...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Marcuzzi A,Zanin V,Kleiner G,Monasta L,Crovella S

    更新日期:2013-09-01 00:00:00

  • Inotropic effects of prostaglandin D2 and E1 on the newborn rabbit heart.

    abstract::This study determines the inotropic effects of prostaglandin D2 (PGD2) and prostaglandin E1 (PGE1) in the isolated, arterial perfused newborn (NB) and adult (A) rabbit heart. Significant positive inotropism of PGD2 was observed at all concentrations studied (1 X 10(-17) to 1 X 10(-7) M) in the two age groups; the effe...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Uemura S,Nakanishi T,Matsuoka S,Friedman WF,Jarmakani JM

    更新日期:1984-12-01 00:00:00

  • High-density lipoprotein subclass distribution in premature newborns before and after the onset of enteral feeding.

    abstract::Changes in high-density lipoprotein (HDL) subclass distribution were evaluated in a group of premature infants during the early postnatal period to ascertain whether enteral feeding brought about a rapid shift from neonatal to adult-like distributions. All infants were fed a combination of breast milk and formula. Cor...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Genzel-Boroviczény O,D'Harlingue AE,Kao LC,Scott C,Forte TM

    更新日期:1988-06-01 00:00:00

  • Atrial myocardial blood flow during acute right ventricular pressure load and adenosine infusion in late gestation fetal sheep.

    abstract::The purpose of this study was to investigate atrial myocardial blood flow in the fetus under conditions of acute right ventricular pressure load and adenosine infusion. Late gestation fetal sheep were instrumented for acute right ventricular pressure loading or adenosine infusion, and regional myocardial blood flow wa...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Lohr JL,Reller MD,Morton MJ,Wu DE,Thornburg KL

    更新日期:1994-03-01 00:00:00

  • Identification of increased expression of activating Fc receptors and novel findings regarding distinct IgE and IgM receptors in Kawasaki disease.

    abstract:BACKGROUND:Kawasaki disease (KD) is associated with expression and methylation of Fc gamma receptor genes. We characterized immunoglobulin A (IgA), IgE, IgG, and IgM receptor expression levels in KD. METHODS:Fc receptor expression levels were characterized using GeneChip Human Transcriptome Array 2.0 (HTA 2.0) with 18...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Chang LS,Ming-Huey Guo M,Lo MH,Kuo HC

    更新日期:2019-12-09 00:00:00

  • Quantitative evaluation of the extent of hepatic enzyme changes in Reye syndrome compared with normal liver or with non-Reye liver disorders: objective criteria for animal models.

    abstract::Five enzymes were measured in 50 liver specimens (18 normal liver, 20 Reye liver, 12 diverse liver disorders other than Reye syndrome). The enzymes were: glutamic dehydrogenase (E.C., monoamine oxidase (E.C., lactate dehydrogenase (E.C., D-glucose-6-phosphate dehydrogenase (E.C., ...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Mitchell RA,Arcinue EL,Partin JC,Partin JS,Ram ML,Chang CH,Smialek J,Sarnaik A

    更新日期:1985-01-01 00:00:00

  • Influence of age on the selenium status in Belgium and The Netherlands.

    abstract::Plasma selenium concentration and glutathione peroxidase activity in red blood cells were determined in subjects from different age groups. The selenium level (mean +/- SD) found in infancy (0 to 6 months) was 2 +/- 0.6 micrograms/dl, with the lowest value of 1 microgram/dl observed in a 4-month-old infant. These leve...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: van Caillie-Bertrand M,Degenhart HJ,Fernandes J

    更新日期:1986-06-01 00:00:00

  • Glutaric aciduria type II: in vitro studies on substrate oxidation, acyl-CoA dehydrogenases, and electron-transferring flavoprotein in cultured skin fibroblasts.

    abstract::The previous biochemical evidence had suggested that glutaric aciduria type II (GA II) is due to deficient dehydrogenation of multiple short-chain acyl coenzyme A's (CoA's), bu the precise biochemical mechanism underlying this disease was unknown. We investigated substrate oxidation and in vitro activities of isovaler...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Rhead W,Mantagos S,Tanaka K

    更新日期:1980-12-01 00:00:00

  • Prolonged survival in hereditary surfactant protein B (SP-B) deficiency associated with a novel splicing mutation.

    abstract::Hereditary surfactant protein B (SP-B) deficiency has been lethal in the first year of life without lung transplantation. We tested the hypothesis that SP-B gene mutations may result in milder phenotypes by investigating the mechanisms for lung disease in two children with less severe symptoms than have been previousl...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Dunbar AE 3rd,Wert SE,Ikegami M,Whitsett JA,Hamvas A,White FV,Piedboeuf B,Jobin C,Guttentag S,Nogee LM

    更新日期:2000-09-01 00:00:00

  • myo-inositol: a newly identified nonnitrogenous osmoregulatory molecule in mammalian brain.

    abstract::Sugar alcohols have been found to play an important osmoregulatory role both in unicellular organisms and, more recently, in multicellular organisms, including mammals. This study shows that myo-inositol accumulates in the brains of chronically hypernatremic mice, as had been earlier found in rats, and demonstrates fo...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Thurston JH,Sherman WR,Hauhart RE,Kloepper RF

    更新日期:1989-11-01 00:00:00

  • Ischemia-reperfusion injury in the intestines of newborn pigs.

    abstract::Although the pathogenesis of necrotizing enterocolitis remains uncertain, ischemia appears to be an important contributing factor to the development of this disorder. Reperfusion plays a major role in ischemia-related injury, and oxygen free radicals produced during reperfusion most likely contribute to the injury. Th...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Papparella A,DeLuca FG,Oyer CE,Pinar H,Stonestreet BS

    更新日期:1997-08-01 00:00:00

  • Dietary lecithin protects against cholestatic liver disease in cholic acid-fed Abcb4- deficient mice.

    abstract::Mutations in multidrug resistance 3 gene (MDR3 or ABCB4) underlie progressive familial intrahepatic cholestasis type 3 (PFIC3), a severe pediatric liver disease progressing to cirrhosis. Abcb4-/- mice exhibit slowly developing hepatic lesions that can be accelerated by feeding a cholic acid (CA)-supplemented diet. We ...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Lamireau T,Bouchard G,Yousef IM,Clouzeau-Girard H,Rosenbaum J,Desmoulière A,Tuchweber B

    更新日期:2007-02-01 00:00:00

  • Ontogeny of growth hormone releasing hormone and insulin-like growth factors-I and -II messenger RNA in rat placenta.

    abstract::Hypothalamic growth hormone releasing hormone (GHRH) stimulates pituitary growth hormone secretion, which is essential for normal postnatal growth. Reports of an immunoreactive and biologically active GHRH-like peptide in placenta led us to investigate placental expression of GHRH mRNA. Placentas from d 19 gestation f...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Pescovitz OH,Johnson NB,Berry SA

    更新日期:1991-05-01 00:00:00

  • Effects of endothelin on renal function in newborn rabbits.

    abstract::The renal effects of endothelin-1 were investigated in 16 anesthetized and mechanically ventilated newborn rabbits. Renal blood flow and glomerular filtration rate were determined by the clearance of para-aminohippuric acid and inulin, respectively. Each animal acted as its own control. In eight newborn rabbits, a bol...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Semama DS,Thonney M,Guignard JP,Gouyon JB

    更新日期:1993-08-01 00:00:00

  • Postganglionic sympathetic discharge in neonatal swine.

    abstract::We hypothesized that cardiac and respiratory modulation of postganglionic peroneal activity appeared in an age-related manner. In anesthetized, paralyzed and artificially ventilated piglets, simultaneous recordings of efferent phrenic and peroneal discharges were obtained during hyperoxia (fraction of inspired oxygen,...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Sica AL,Hundley BW,Gootman PM

    更新日期:1996-01-01 00:00:00

  • Immunologic studies of arylsulfatase A in normal and metachromatic leukodystrophy liver.

    abstract::Purified human liver arylsulfatase A on polyacrylamide gel electrophoresis at pH 4.0 is separated into two protein forms with enzymatic activity and two distinct inactive subunits. All of these components were immunologically distinguishable using different antisera preparations. In late infantile metachromatic leukod...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Shapira E,DeGregorio RR,Nadler HL

    更新日期:1978-03-01 00:00:00

  • Experimental study of coil embolization using a new atelocollagen spring coil.

    abstract::Spring steel coils have been used to occlude abnormal vessels in patients with a congenital heart malformation. However, long-term adverse effects of steel spring coils remain uncertain, although some long-term results appear to be good. The purpose of this study was to evaluate the angiographic and histologic results...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Ino T,Kishiro M,Yamashiro Y,Tanaka A,Ito H

    更新日期:1998-04-01 00:00:00

  • Growth velocity and plasma amino acids in the newborn.

    abstract::Plasma amino acid levels in newborn babies are often measured as part of a screening for inherited metabolic disease, or less commonly as part of a nutritional assessment. We have examined certain factors which affect the levels as measured in low birth weight babies during the first 3 wk of life. As in other studies,...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Scott PH,Berger HM,Wharton BA

    更新日期:1985-05-01 00:00:00

  • Growth hormone-releasing hexapeptide is a potent stimulator of growth hormone gene expression and release in the growth hormone-releasing hormone-deprived infant rat.

    abstract::The growth hormone-releasing hexapeptide (GHRP-6) specifically stimulates growth hormone (GH) secretion in several animal species and humans. The mechanism of action of GHRP-6 is largely unknown, although experimental evidence indicates that it may modulate growth hormone-releasing hormone (GHRH) and somatostatin acti...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Locatelli V,Grilli R,Torsello A,Cella SG,Wehrenberg WB,Müller EE

    更新日期:1994-08-01 00:00:00

  • The role of neutrophils in the production of hypoxic-ischemic brain injury in the neonatal rat.

    abstract::Neutrophils contribute to ischemic brain injury in adult animals. The role of neutrophils in perinatal hypoxic-ischemic (HI) brain injury is unknown. Allopurinol reduces neutrophil accumulation after tissue ischemia and is protective against HI brain injury. This study was designed to investigate how neutrophils contr...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Hudome S,Palmer C,Roberts RL,Mauger D,Housman C,Towfighi J

    更新日期:1997-05-01 00:00:00

  • Infant body composition relationship to maternal adipokines and fat mass: the PONCH study.

    abstract:BACKGROUND:Infant adiposity is linked to both high maternal fat mass (FM) and excessive gestational FM gain, whereas the association with maternal adipokines is less clear. The aim was to determine how levels of maternal leptin, the soluble leptin receptor (sOB-R), adiponectin, and FM during pregnancy were linked to in...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Andersson-Hall UK,Pivodic A,de Maré HK,Pernilla Svedin J,Carina Mallard E,Albertsson-Wikland KG,Holmäng AB

    更新日期:2020-09-14 00:00:00

  • Differential effects of cholesterol and budesonide on biophysical properties of clinical surfactant.

    abstract:INTRODUCTION:Corticosteroids have been widely used in clinical medicine as a first-line therapy to modify the inflammatory response in many pulmonary and systemic diseases. Inhaled and intratracheally administered corticosteroids have a particular interest in that their use allows the clinician to circumvent systemic s...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Zhang H,Wang YE,Neal CR,Zuo YY

    更新日期:2012-04-01 00:00:00