Glycolytic metabolism of neonatal mononuclear cells.

Abstract:

:The mononuclear cells of newborn infants compared to those of normal adults contain diminished activity of pyruvate kinase (PK) (14.9 +/- 1.2 vs. 22.3 +/- 1.3 mumol/10(10) cells/min) and phosphoglycerate kinase (PGK) (195 +/- 10 vs. 415 +/- 19). These deficiencies are accentuated in the cells of premature infants (4.9 +/- 0,5 and 71 +/- 15, respectively) and are accompanied by reduced activity of adenylate kinase (AK) (14.8 +/- 1.9 vs. 33.6 +/- 2.6). The activities of AK and PGK in adult and neonatal mononuclear cells are stimulated by phytohemagglutinin (PHA). Although the PK activity of adult cells is stimulated by PHA, the PK activity of neonatal cells is not. Mononuclear cell glycolysis is stimulated by PHA in both adults and neonates. The mean lactate production of neonatal cells is slightly lower than it is in adult cells (93 vs. 110 mumol/10(10) cells/hr, unstimulated, and 151 vs. 181, stimulated), but the differences are not statistically significant. The ATP content of neonatal cells (3.8 +/- 0.4 mumol/ 10(10) cells) is reduced compared to adult values (6.0 +/- 0.3). Lactate production is directly correlated with PK activity in adult and neonatal mononuclear cells. Neonatal mononuclear cell PK activity increases to adult levels during the first year of life.

journal_name

Pediatr Res

journal_title

Pediatric research

authors

Das M,Klein W,Feig SA

doi

10.1203/00006450-197710000-00008

subject

Has Abstract

pub_date

1977-10-01 00:00:00

pages

1068-72

issue

10 Pt 1

eissn

0031-3998

issn

1530-0447

journal_volume

11

pub_type

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