Immunolocalization of cellular fibronectins in the normal liver, cirrhosis, and hepatocellular carcinoma.

Abstract:

:Cellular (c) fibronectins (Fn) differ biochemically, immunologically, and functionally from plasma fibronectins (pFn). Most existing data on Fn distribution in the normal and diseased liver require revision because those studies were based on reagents that did not distinguish pFn from cFn and predated the development of specific cFn monoclonal antibodies (Mabs). We immunostained cryosections of normal adult livers (n = 5), cirrhotic livers (n = 20), and livers with hepatocellular carcinoma (HCC) (n = 10) by the avidin-biotin-complex method with specific Mabs to the extradomains A and B (EDA, EDB) and oncofetal (Onc) isoforms of cFn. Selected samples were stained with an antiserum to pFn; fetal livers served as controls. Normal and cirrhotic livers showed EDA-cFn staining in the portal, septal, and perisinusoidal matrix; its distribution was more restricted than that of pFn. In cirrhosis, EDA-cFn reactions were strongest at sites of piecemeal necrosis and around proliferating ductules in biliary cirrhosis. EDA-cFn reactions were consistently most intense in the matrix of HCC. Distinct from adult normal and cirrhotic livers, reactions for EDB- and Onc-cFn were noted exclusively in most cases of HCC. We conclude that the only cFn isoform indigenous to the normal adult liver matrix is EDA-cFn. Enhanced EDA-cFn in cirrhotic livers may serve as indicator of active stromal remodeling. The restriction of EDB- and Onc-cFn to a large subset of HCC and the putative role of cFn in modulating cell-matrix adhesive interactions would suggest that the emergence of these molecules may be related to the variably invasive and metastatic properties of these tumors.

journal_name

Ultrastruct Pathol

authors

Koukoulis GK,Shen J,Virtanen I,Gould VE

doi

10.3109/01913129509014601

subject

Has Abstract

pub_date

1995-01-01 00:00:00

pages

37-43

issue

1

eissn

0191-3123

issn

1521-0758

journal_volume

19

pub_type

杂志文章
  • Clear cell leiomyoma (leiomyoblastoma) of the uterus: ultrastructural observations.

    abstract::The ultrastructure of a benign uterine epithelioid leiomyoma with prominent clear cells ("leiomyoblastoma") was studied to define the subcellular changes that yield the distinctive light microscopic pattern of this rare lesion. The main constituent of the cytoplasm was multiple cytolysosomes composed of vesicular, mem...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913128609032224

    authors: Mazur MT,Priest JB

    更新日期:1986-01-01 00:00:00

  • Differences in nuclear pore density among human choroidal melanoma cell types.

    abstract::Two cases of mixed cell type choroidal melanoma were examined by means of conventional and freeze-fracture electron microscopy. Data on average nuclear pore density were readily collected from freeze-fracture replicas of spindle A, spindle B, and epithelioid cells. Highly significant (p less than 0.001) differences in...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913129009032246

    authors: Sacks-Wilner R,Freddo TF

    更新日期:1990-07-01 00:00:00

  • Astroblastoma: ultrastructural observations on a case of high-grade type.

    abstract::An astroblastoma of high-grade type arising in the brain of a 3-year-old child is reported. The first description of the ultrastructural, immunohistochemical, and cytogenetic findings in this rare tumor variant are presented. ...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/019131299281473

    authors: Mierau GW,Tyson RW,McGavran L,Parker NB,Partington MD

    更新日期:1999-09-01 00:00:00

  • Histological and biochemical effects of dexmedetomidine on liver during an inflammatory bowel disease.

    abstract::Inflammation in the liver is an extraintestinal manifestation that is frequently seen during inflammatory bowel diseases (IBD). The authors investigated histopathologycal, ultrastructural and antioxidant effects of dexmedetomidine (Dex) on liver during trinitrobenzene sulfonic acid (TNBS)-induced inflammatory bowel di...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913123.2013.829150

    authors: Gul M,Kayhan B,Elbe H,Dogan Z,Otlu A

    更新日期:2015-02-01 00:00:00

  • Histological and ultrastructural evaluation of human decellularized matrix as a hernia repair device.

    abstract::Recently, interest has been increasing for human decellularized matrices, due to their ability to reduce numerous side effects related to hernia repair. To date, only animal studies investigated the biological interaction post-implant of human decellularized matrices for soft tissue repair. Therefore, the aim of this ...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/01913123.2017.1365788

    authors: Ghetti M,Papa V,Deluca G,Purpura V,Ruscelli P,Melandri D,Capirossi D,Nigrisoli E,Minghetti P,Bondioli E,Cenacchi G

    更新日期:2018-01-01 00:00:00

  • Ribosome-lamella complex of the lymphocytes in a case of plasmacytic lymphadenopathy with polyclonal hypergammaglobulinemia.

    abstract::A case of lymphoid hyperplasia with prominent plasmacytic proliferation histologically and with polyclonal hypergammaglobulinemia and anemia clinically was reported. This condition in a 54-year-old Japanese male was probably a type of plasma cell dyscrasia similar to a plasma cell variant of Castleman's disease. About...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913128409018570

    authors: Mikata A,Suzuki S,Suzuki H,Higo O,Shimoyama M

    更新日期:1984-01-01 00:00:00

  • A new tool improves diagnostic test performance for transmission em evaluation of axonemal dynein arms.

    abstract::Abstract Diagnosis of primary ciliary dyskinesia (PCD) by identification of dynein arm loss in transmission electron microscopy (TEM) images can be confounded by high background noise due to random electron-dense material within the ciliary matrix, leading to diagnostic uncertainty even for experienced morphologists. ...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章,随机对照试验

    doi:10.3109/01913123.2013.815081

    authors: Funkhouser WK 3rd,Niethammer M,Carson JL,Burns KA,Knowles MR,Leigh MW,Zariwala MA,Funkhouser WK Jr

    更新日期:2014-08-01 00:00:00

  • Alpha and beta subunits of glycoprotein hormones in argyrophil pituitary tumors with small granule cells.

    abstract::A group of 33 functionless pituitary tumors with small argyrophil groups (SAG) were collected from a series of 200 pituitary adenomas (16.5% of all adenomas). Histologically, the tumors showed an unusually high frequency of trabecular patterns, perivascular pseudo-rosettes, and oncocytoid transformation. Immunoreactiv...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913128309140570

    authors: Capella C,Buffa R,Usellini L,Frigerio B,Jehenson P,Sessa F,Solcia E

    更新日期:1983-01-01 00:00:00

  • Swim exercise training ameliorates hepatocyte ultrastructural alterations in rats fed on a high fat and sugar diet.

    abstract::Excessive consumption of carbohydrate and fat increases the risk of liver disease. We hypothesized that swim exercise can protect hepatocytes from ultra-structural damage induced by high cholesterol and fructose diets (HCFD). Rats were either fed with HCFD (model group) or a standard laboratory chow (control group) fo...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/01913123.2017.1422581

    authors: Dallak MA,Bin-Jaliah I,Albawardi A,Haidara MA,Sakr HF,Eid RA,Hassan WN,Al-Ani B

    更新日期:2018-03-01 00:00:00

  • Sarcomatous type of malignant mesothelioma.

    abstract::Thirteen malignant mesotheliomas of a sarcomatous type were studied by light microscopy and ten were studied by electron microscopy. The histologic patterns varied from tumor to tumor, often resembling other soft tissue sarcomas. Electron microscopic observation showed most of the tumors to be composed of primitive ce...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913128309140587

    authors: Klima M,Bossart MI

    更新日期:1983-06-01 00:00:00

  • Fetal calf serum versus human serum: ultrastructural evaluation of protein support influence on human ovarian tissue cryopreservation.

    abstract::The aim of this study was to examine the effect of the different protein supports in the cryopreservation solution on improving human ovarian tissue preservation after frozen-thawed procedures. Biopsies of ovarian cortical tissue were obtained from 14 subjects. All specimens were cryopreserved using a slow freezing/ra...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/01913120600820187

    authors: Fabbri R,Pasquinelli G,Bracone G,Orrico C,Paradisi R,Seraccioli R,Venturoli S

    更新日期:2006-07-01 00:00:00

  • Intercellular communication by extracellular vesicles with emphasis on the roles of cordocytes in the human brain. An ultrastructural study.

    abstract::We describe in this work the presence of extracellular vesicles (EVs) along different cell types, especially cordocytes, in various clinical conditions of the human brain (atherothrombotic disease, cerebral tumors, hygroma durae matris, intracerebral cysts, Moyamoya disease and parenchymatous hematoma) using transmiss...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913123.2014.981327

    authors: Pais V,Pais ES

    更新日期:2015-05-01 00:00:00

  • Carcinoma versus cytokeratin-positive lymphoma: a case report emphasizing the diagnostic role of electron microscopy.

    abstract::Lymphoma diagnosis rarely needs electron microscopy (EM), but one area where it can be useful is in the distinction of cytokeratin-positive lymphoma from carcinoma. The authors describe such a case, where difficulties were encountered due to lack of antibody specificity, distinguishing reactive from tumoral cells, and...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/01913120802625830

    authors: Eyden B,Chakrabarty B,Hatimy U

    更新日期:2009-01-01 00:00:00

  • The effects of X-ray irradiation on the proliferation and apoptosis of MCF-7 breast cancer cells.

    abstract::To investigate the effects of X-ray irradiation on the proliferation and apoptosis of MCF-7 breast cancer cells; MCF-7 breast cancer cells were irradiated with X-ray. After irradiation, morphological changes and growth inhibition rate of the irradiated cells were observed under an inverted microscope. 3-(4,5-dimethylt...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913123.2013.861569

    authors: Li DL,Wei L,Wen XM,Song H,Li Q,Lv JW,Kuang CC,Wei ZZ,Zhang JW

    更新日期:2014-05-01 00:00:00

  • Heterogeneity of malignant schwannomas.

    abstract::Six malignant schwannomas were studied by electron microscopy and immunohistochemistry for S-100 protein at the light and electron microscopic levels to clarify the nature of the tumor cells. Three tumors (group A) were composed of poorly differentiated tumor cells and showed no immunoreactivity for S-100 protein. One...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913128809048479

    authors: Hirose T,Sano T,Hizawa K

    更新日期:1988-01-01 00:00:00

  • Large block embedding and "pop-off" technique for immunoelectron microscopy.

    abstract::A technique for immunoelectron microscopy is described whereby tissue is embedded in large (6 x 12 mm) plastic blocks and reembedded ("popped off") directly from semithin (3-microns) sections for thin sectioning. This technique is particularly useful in locating relatively sparse cells or tissue structures. Three acry...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913129209074550

    authors: de Mesy Jensen KL,di Sant'Agnese PA

    更新日期:1992-01-01 00:00:00

  • Epididymal ultrastructural changes associated with chronic cholestasis after bile duct ligation in adult rats.

    abstract::Testicular atrophy and testesterone insufficiency have been commonly reported associated with chronic liver diseases. Though testosterone dependent, the epididymal changes induced by liver disease have never been studied before. Thus, this study aimed to assess the ultrastructural events in the epididymis of rats with...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/01913123.2018.1488789

    authors: Mahmoud YI,Abo-Zeid FS

    更新日期:2018-07-01 00:00:00

  • Ultrastructural effects of recombinant gamma-interferon on cultured human keratinocytes.

    abstract::To extend our initial observations that recombinant gamma-interferon (r gamma IFN) influences the growth and differentiation of normal cultured human keratinocytes, we studied the electron microscopic changes induced by r gamma IFN. Treatment of cultured human keratinocytes with 10(3) units/ml (7.1 nM) of r gamma IFN ...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913128609015559

    authors: Nickoloff BJ,Mahrle G,Morhenn V

    更新日期:1986-01-01 00:00:00

  • Distinguishing B and T lymphocytes by scanning electron microscopy.

    abstract::This work demonstrates differences between B lymphocytes and T lymphocytes as seen in the scanning electron microscope (SEM). Slides of routinely prepared benign and malignant lymphoid tissues were viewed in the SEM. The location of prominent populations of T lymphocytes and B lymphocytes was verified by immunocytoche...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/01913120050176653

    authors: Terzakis JA

    更新日期:2000-07-01 00:00:00

  • Value of electron microscopy in the diagnosis of glomerular diseases.

    abstract::To evaluate the contribution of electron microscopy to the final diagnosis of glomerulopathies, the authors established a prospective study during the first semester of 2006. A total of 52 kidney biopsies were performed with 3 samples for light microscopy, immunofluorescence, and electron microscopy. Among these renal...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913120903506637

    authors: Darouich S,Goucha RL,Jaafoura MH,Moussa FB,Zekri S,Maiz HB

    更新日期:2010-04-01 00:00:00

  • Glial fibrillary acidic protein (GFAP) immunoreactivity in peripheral nerve sheath tumors.

    abstract::A spectrum of 24 benign and malignant nerve sheath tumors and 10 non-neural spindle-cell tumors were studied by light microscopy for the presence of glial fibrillary acidic protein (GFAP) immunoreactivity by the peroxidase-antiperoxidase (PAP) technique. In 8 cases, these results were compared to their electron micros...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913128409141487

    authors: Memoli VA,Brown EF,Gould VE

    更新日期:1984-01-01 00:00:00

  • Ultrastructure of HIV/AIDS.

    abstract::Transmission electron microscopy has played a key role in our understanding of the human immunodeficiency virus and the opportunistic infections that accompany HIV disease. This paper describes features of HIV production; HHV-8, the virus that is associated with Kaposi sarcoma; Trachipleistophora anthropophthera, a ne...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章,评审

    doi:10.1080/01913120290104502

    authors: Orenstein JM

    更新日期:2002-07-01 00:00:00

  • CD44s and CD44v6 in diagnosis and prognosis of human bladder cancer.

    abstract:UNLABELLED:The cell adhesion molecules (CAMs) CD44 standard (CD44s) and its variant 6 (CD44v6) are involved in the progression and invasion of human malignancies. However, discrepancies in the prognostic value of CD44s and CD44v6 expression need to be addressed. AIMS:To investigate the expression of CD44s and CD44v6 i...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913123.2011.651522

    authors: Omran OM,Ata HS

    更新日期:2012-05-01 00:00:00

  • Correlation of proteinuria with podocyte foot process effacement in IgA nephropathy: an ultrastructural study.

    abstract:BACKGROUND:Proteinuria is an uncommon clinical manifestation of IgA nephropathy and is usually seen in cases with severe lesions like endocapillary proliferation. However, it is occasionally seen even with cases with mild glomerular manifestations and may even be of nephrotic range. PREDICTOR:Podocyte foot process eff...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913123.2014.960543

    authors: Tewari R,Nada R,Rayat CS,Boruah D,Dudeja P,Joshi K,Sakhuja V

    更新日期:2015-04-01 00:00:00

  • Ameliorative effect of Pimpinella anisum oil on immunohistochemical and ultrastuctural changes of cerebellum of albino rats induced by aspartame.

    abstract::The study aims to investigate the protective effect of Pimpinella anisum oil on aspartame (ASP) which resulted in cerebellar changes. The rats were divided into four equal groups: Group 1: (control group): served as control animals. Group 2: control P. anisum oil received .5 mL/kg/d/b wt. once daily. Group 3 (ASP grou...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913123.2014.889259

    authors: Abdul-Hamid M,Gallaly SR

    更新日期:2014-05-01 00:00:00

  • Prolonged storage of fixative for electron microscopy: effects on tissue preservation for diagnostic specimens.

    abstract::Prolonged storage of Karnovsky's fixative, at both 4 and -20 degrees C, is possible in the diagnostic electron microscopy setting. Ultrastructural detail was not compromised in specimens processed in fixative that had been stored for 6 months. Evidence of smooth muscle and neuroendocrine differentiation was present in...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913129709021318

    authors: Mount SL,Schwarz JE,Taatjes DJ

    更新日期:1997-03-01 00:00:00

  • Jaw osteonecrosis in patients treated with bisphosphonates: an ultrastructural study.

    abstract::Osteonecrosis of the jaw is a severe bone disorder traditionally associated with periodontal disease, local malignancy, chemotherapy, glucocorticoid therapy, or trauma. Recently a growing number of publications reported the occurrence of osteonecrosis of the jaw in patients undergoing treatment with bisphosphonates. T...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913121003729806

    authors: Perrotta I,Cristofaro MG,Amantea M,Russo E,De Fazio S,Zuccalà V,Conforti F,Amorosi A,Donato G,Tripepi S,Giudice M

    更新日期:2010-08-01 00:00:00

  • Primary cilia metaplasia in renal transplant biopsies with acute tubular injury.

    abstract::Primary cilia are hair-like organelles singly distributed along the apical surface of proximal and distal nephron tubules as mechanosensors. The goal of this study was to use electron microscopy to systemically evaluate cilia changes in acute tubular injury (ATI) from both transplant and native renal biopsies. Three g...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913123.2013.768745

    authors: Hayek S,Parasuraman R,Desai HS,Samarapungavan D,Li W,Wolforth SC,Reddy GH,Cohn SR,Rocher LL,Dumler F,Rooney MT,Zhang PL

    更新日期:2013-05-01 00:00:00

  • Confocal laser scanning microscopy of liesegang rings in odontogenic cysts: analysis of three-dimensional image reconstruction.

    abstract::Liesegang rings are concentric noncellular lamellar structures, occasionally found in inflammatory tissues. They have been confused with various parasites, algas, calcification, and psammoma bodies. The authors examined Liesegang rings from oral inflammatory cysts by both optical and confocal laser scanning microscopy...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/01913120902751288

    authors: Scivetti M,Lucchese A,Crincoli V,Pilolli GP,Favia G

    更新日期:2009-03-01 00:00:00

  • Neutrophil fate in gingival crevicular fluid.

    abstract::The fate of the neutrophils within the inflammatory exudate in the periodontal crevice and their possible participation in the formation of neutrophil extracellular traps (NETs) are of clinical interest. However, the cytological analysis of clinical samples of inflammatory exudate is restricted by the obtainable quant...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913120903419989

    authors: Vitkov L,Klappacher M,Hannig M,Krautgartner WD

    更新日期:2010-02-01 00:00:00