Prolonged storage of fixative for electron microscopy: effects on tissue preservation for diagnostic specimens.

Abstract:

:Prolonged storage of Karnovsky's fixative, at both 4 and -20 degrees C, is possible in the diagnostic electron microscopy setting. Ultrastructural detail was not compromised in specimens processed in fixative that had been stored for 6 months. Evidence of smooth muscle and neuroendocrine differentiation was present in the form of actin filaments/dense bodies and neurosecretory granules, respectively. No difference in preservation was detected between specimens fixed in freshly prepared Karnovsky's fixative and fixative that had been stored at either 4 or -20 degrees C for up to 6 months. Thus, community hospitals can be provided with Karnovsky's fixative on a semiannual basis for surgical pathology specimens that may require electron microscopy for diagnosis.

journal_name

Ultrastruct Pathol

authors

Mount SL,Schwarz JE,Taatjes DJ

doi

10.3109/01913129709021318

subject

Has Abstract

pub_date

1997-03-01 00:00:00

pages

195-200

issue

2

eissn

0191-3123

issn

1521-0758

journal_volume

21

pub_type

杂志文章
  • Insular amyloid in a case of type III glycogenosis with a special reference to the origin of amyloid fibrils.

    abstract::Amyloid depositions of pancreatic islets were investigated with electron microscopy in a case of type III glycogenosis. Beta cells adjoining small amyloid depositions were shown to have cytoplasmic invaginations where closely packed amyloid fibrils were disclosed regularly orientated amyloid bundles. In the cytoplasm ...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913128609032222

    authors: Takahashi M,Yokota T,Ishihara T,Uchino F,Kamei T

    更新日期:1986-01-01 00:00:00

  • Disseminated tubuloreticular inclusions in acquired immunodeficiency syndrome (AIDS).

    abstract::Tissue biopsies and peripheral blood samples from 10 patients with the characteristic clinical features of acquired immunodeficiency syndrome (AIDS) were examined by electron microscopy and ultracytochemical myeloperoxidase technique. Abundant tubuloreticular inclusions (TRI) were detected within the endoplasmic retic...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913128309140585

    authors: Kostianovsky M,Kang YH,Grimley PM

    更新日期:1983-06-01 00:00:00

  • Paraproteinemic crystalloidal keratopathy: an ultrastructural study of two cases, including immunoelectron microscopy.

    abstract::The ultrastructural appearances of corneal crystalloidal deposits are described in two patients with an IgG-kappa paraproteinemia of uncertain pathogenesis. The crystalloids in one patient were overwhelmingly intracellular and were found mainly in stromal keratocytes, but also in basal corneal epithelial cells and the...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章,评审

    doi:10.3109/01913129309027800

    authors: Henderson DW,Stirling JW,Lipsett J,Rozenbilds MA,Roberts-Thomson PJ,Coster DJ

    更新日期:1993-11-01 00:00:00

  • An ultrastructural and immunohistochemical study of elastofibroma: CD 34, MEF-2, prominin 2 (CD133), and factor XIIIa-positive proliferating fibroblastic stromal cells connected by Cx43-type gap junctions.

    abstract::Elastofibromas have been described as ill-defined tumors, composed of fibroblastic stromal cells and a dense collagenous stroma. A total of 5 elastofibromas from 4 Japanese patients were examined by ultrastructural and immunohistochemical methods. The proliferating fibroblastic stromal cells in the lesion showed Cx43-...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/01913120701350365

    authors: Yamazaki K

    更新日期:2007-05-01 00:00:00

  • Ultrastructural pathology of human immunodeficiency virus infection.

    abstract::The electron microscope has been used with great skill in many aspects of the acquired immunodeficiency syndrome. It has played a critical role in classifying the human immunodeficiency virus, in characterizing the morphogenesis and gene products of the virus, and in elucidating the host cell targets and interactions....

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章,评审

    doi:10.3109/01913129209074561

    authors: Orenstein JM

    更新日期:1992-01-01 00:00:00

  • Comparison of the myotoxic effects of levobupivacaine, bupivacaine, and ropivacaine: an electron microscopic study.

    abstract::The aim of this study was to investigate the myotoxic effects of bupivacaine, ropivacaine, and levobupivacaine which were applied intramuscularly to rat skeletal muscle. Forty Wistar-Albino rats were divided into four groups. In the study, .5% bupivacaine (Group B), .5% ropivacaine (Group R), .5% levobupivacaine (Grou...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913123.2015.1014610

    authors: Öz Gergin Ö,Yıldız K,Bayram A,Sencar L,Coşkun G,Yay A,Biçer C,Özdamar S,Polat S

    更新日期:2015-05-01 00:00:00

  • Lithium effects on vesicular trafficking in hepatocellular carcinoma cells.

    abstract::Hepatocellular carcinoma (HCC) is one of the most commonly malignant tumors worldwide, characterized by the presence of many heterogeneous molecular cell events that contribute to tumor growth and progression. Endocytic processes are intimately involved in various pathological conditions, including cancer, since they ...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/01913123.2019.1701167

    authors: Taskaeva I,Bgatova N,Gogaeva I

    更新日期:2019-01-01 00:00:00

  • Bednar tumor: report of a case with immunohistochemical and ultrastructural study.

    abstract::A slowly growing tumor in the right shoulder of a 38-year-old white male, which felt like a superficial cystic mass, was studied. The spindle cells, which represented the main component of the tumor, were arranged in a typical storiform pattern and were positive for CD34 and focally for CD117. The pigmented cells were...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/01913120309935

    authors: Seo IS,Goheen M,Min KW

    更新日期:2003-05-01 00:00:00

  • Deciduoid mesothelioma: a report of 5 cases and literature review.

    abstract::Deciduoid mesothelioma was first described in young females and in the peritoneum, which led to the suggestion that deciduoid mesothelioma was a distinct subtype with specific clinical and pathologic features. Later reports, however, have shown that this type of mesothelioma may also occur in elderly people and in the...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章,评审

    doi:10.1080/0913120290104647

    authors: Shia J,Erlandson RA,Klimstra DS

    更新日期:2002-11-01 00:00:00

  • 5'-CpG island methylation of the FHIT gene is associated with reduced protein expression and higher clinical stage in cervical carcinomas.

    abstract::Aberrant transcription of the FHIT gene has been observed in many solid tumors, suggesting that 5'-CpG island methylation of the FHIT gene is involved in tumor development. The authors investigated the status of the 5'-CpG island methylation of the FHIT gene and protein expression in a series of 40 cervical carcinomas...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/01913120390250329

    authors: Wu Q,Shi H,Suo Z,Nesland JM

    更新日期:2003-11-01 00:00:00

  • Extrusion of amyloid fibrils to the extracellular space in experimental mesangial AL-amyloidosis: transmission and scanning electron microscopy studies and correlation with renal biopsy observations.

    abstract::In vitro studies have provided much information regarding the process of glomerular AL-amyloidogenesis. Research efforts have been successful in deciphering how glomerulopathic light chains interact with mesangial cells. The sequential steps involved in the genesis of amyloid fibrils include interactions with surface ...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913123.2013.861568

    authors: Teng J,Turbat-Herrera EA,Herrera GA

    更新日期:2014-04-01 00:00:00

  • Ultrastructural, cytochemical, and immunocytochemical study of nuclei and cytoskeleton of thyroid papillary carcinoma cells.

    abstract::One of the most constant and typical histological markers of thyroid papillary carcinoma is the presence of multilobated and ground-glass nuclei. With the intention of extending and characterizing the ultrastructural comprehension of this feature, thyroid papillary carcinoma cells and normal follicular cells were stud...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913129809033471

    authors: Echeverría OM,Hernández-Pando R,Vázquez-Nin GH

    更新日期:1998-05-01 00:00:00

  • SEM Study of apical morphological alterations in primary teeth with vital and necrotic pulps.

    abstract::This study evaluated, by SEM, the morphology of human primary teeth roots. Twenty-four teeth were divided into 3 groups: pulp vitality (group I) and pulp necrosis without (group II) and with apical periodontitis (group III). Roots were analyzed by the presence of periodontal ligament (PDL) fibers and resorption areas....

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913120903275642

    authors: da Silva LA,Fernandes PM,da Silva RA,Nelson-Filho P,Rocha LB,Rossi MA

    更新日期:2009-10-01 00:00:00

  • Diagnostic electron microscopy on reembedded ("popped off") areas of large Spurr epoxy sections.

    abstract::A technique is described in which areas of large Spurr epoxy thick sections are directly popped off of glass slides by inverted Beem capsules filled with polymerized plastic. This technique allows the survey of large regions of tissue with the maintenance of tissue relationships and the selection of specific areas for...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913128409018580

    authors: di Sant'Agnese PA,De Mesy-Jensen KL

    更新日期:1984-01-01 00:00:00

  • The ultrastructure of hybrid acute leukemia: a study of 15 cases.

    abstract::The objective of this study was to investigate the ultrastructural characteristics of hybrid acute leukemia (HAL). Fifteen cases of HAL were studied by transmission electron microscopy (TEM), focusing on organelles and myeloperoxidase (MPO) reaction of leukemic cells. By TEM, 5 out 15 cases of HAL were consistent with...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/019131290968722

    authors: Ru YX,Wang HJ,Yang BX,Liu JH,Li ZQ,Li CW,Wang JX,Mi YC

    更新日期:2005-09-01 00:00:00

  • Primitive neuroectodermal tumors: ultrastructural and immunohistochemical studies.

    abstract::We report here ultrastructural and immunohistochemical studies of neuroblastic differentiation in the retrospective (n = 17) and prospective (n = 26) series of primitive neuroectodermal tumors (PNETs). By electron microscopy, neuritelike structures containing parallel-oriented microtubules, adhesive plaque junctions, ...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913129509064217

    authors: Papierz W,Alwasiak J,Kolasa P,Wegrzyn Z,Zakrzewski K,Polis L,Debiec-Rychter M,Liberski PP

    更新日期:1995-05-01 00:00:00

  • A fast method for processing biologic material for electron microscopic diagnosis in infectious disease.

    abstract::A fast method for processing biologic material for electron microscopy for precise and specific diagnosis of infectious agents is an increasing necessity. After different, reportedly fast methods were tested, a useful and quick technique was developed that provides well-preserved cellular structures, enabling the etio...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913129209057832

    authors: Duarte MI,Mariano ON,Takakura CF,Everson D,Corbett CE

    更新日期:1992-07-01 00:00:00

  • Ultrastructure of the blood and lymphatic capillaries of the respiratory tissue during inflammation and endobronchial laser therapy.

    abstract::For wide application of low-energy laser irradiation in the pulmonary clinic, study of the structural basis of the therapeutic effect is necessary. The aim of this research is to describe the structural changes of the blood and lymphatic capillaries in the respiratory tissues during inflammation and following laser bi...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/01913120050132921

    authors: Polosukhin VV

    更新日期:2000-05-01 00:00:00

  • Morphologic characteristics of blastic plasmacytoid dendritic cell neoplasm: a case report.

    abstract::Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare aggressive lymphoma derived from plasmacytoid dendritic cells or precursor dendritic cells. Despite some 240 reported cases, its morphology and especially ultrastructure has not been satisfactorily studied. A case is reported of a 13 year old boy, who, des...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913123.2013.813998

    authors: Ru Y,Zhang P,Dong S,Wang H,Zhao S,Mi Y,Eyden B

    更新日期:2014-02-01 00:00:00

  • Gastrointestinal stromal tumor of the stomach with rhabdoid phenotype: immunohistochemical, ultrastructural, and immunoelectron microscopic evaluation.

    abstract::A variety of neoplasms with rhabdoid differentiation have been reported in many sites. The authors describe a case of gastrointestinal stromal tumor (GIST) of the stomach that exhibited prominent rhabdoid features. Immunohistochemically, the tumor cells displayed positive staining for vimentin, c-kit, CD34, and alpha ...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/01913120490475707

    authors: Richmond JA,Mount SL,Schwarz JE

    更新日期:2004-05-01 00:00:00

  • A new tool improves diagnostic test performance for transmission em evaluation of axonemal dynein arms.

    abstract::Abstract Diagnosis of primary ciliary dyskinesia (PCD) by identification of dynein arm loss in transmission electron microscopy (TEM) images can be confounded by high background noise due to random electron-dense material within the ciliary matrix, leading to diagnostic uncertainty even for experienced morphologists. ...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章,随机对照试验

    doi:10.3109/01913123.2013.815081

    authors: Funkhouser WK 3rd,Niethammer M,Carson JL,Burns KA,Knowles MR,Leigh MW,Zariwala MA,Funkhouser WK Jr

    更新日期:2014-08-01 00:00:00

  • Intercellular communication by extracellular vesicles with emphasis on the roles of cordocytes in the human brain. An ultrastructural study.

    abstract::We describe in this work the presence of extracellular vesicles (EVs) along different cell types, especially cordocytes, in various clinical conditions of the human brain (atherothrombotic disease, cerebral tumors, hygroma durae matris, intracerebral cysts, Moyamoya disease and parenchymatous hematoma) using transmiss...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913123.2014.981327

    authors: Pais V,Pais ES

    更新日期:2015-05-01 00:00:00

  • Adult neuroblastoma of bone: a case report.

    abstract::Adult neuroblastoma is an uncommon tumor and it is rare for one to present as a primary bone lesion. We report the case of a 23-year-old man in whom the tumor initially appeared in the right scapula. Light microscopic, immunocytochemical, and electron microscopic findings were typical of neuroblastoma. ...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913128709048440

    authors: Mackay B,Ordóñez NG

    更新日期:1987-01-01 00:00:00

  • An electron microscopic study of central nervous system sarcoidosis.

    abstract::Although the fine structure of extraneural sarcoidosis is well documented, ultrastructural study of central nervous system (CNS) sarcoidosis has been limited. Electron microscopic (EM) examination of 3 biopsy cases of CNS sarcoid revealed a mixed cellular population of lymphocytes, epitheloid cells, and multinucleated...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913128109048309

    authors: Trombley IK,Mirra SS,Miles ML

    更新日期:1981-07-01 00:00:00

  • Ultrastructural aspect of myocarditis: its relevance for the diagnosis.

    abstract::The Dallas consensus was used to reveal active or borderline inflammatory loci by light microscopy (LM). When lymphocyte-cardiocyte interaction was observed by electron microscopy (EM), the deleterious or dormant pattern of inflammatory process was recognized. The first was determined by lymphocytes that adhered to ca...

    journal_title:Ultrastructural pathology

    pub_type: 临床试验,杂志文章

    doi:10.3109/01913129509014620

    authors: Milin J,Stojsić D,Vucković D,Benc D,Hadzić M,Stojsić A,Zivkov-Saponja D

    更新日期:1995-11-01 00:00:00

  • Sarcomatoid renal cell carcinoma metastatic to the heart: report of a case.

    abstract::An unusual case of metastatic sarcomatoid renal cell carcinoma is presented. Fifteen months after nephrectomy for a typical clear cell carcinoma, a 63-year-old man presented with bilateral pleural effusions, cardiomegaly, and tamponade. A pericardial biopsy showed an anaplastic spindle cell tumor that was strongly ker...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913129109016245

    authors: Bird DJ,Semple JP,Seiler MW

    更新日期:1991-07-01 00:00:00

  • Reduplicated basal lamina in clear-cell carcinoma of the ovary: an immunohistochemical and electron microscopic study.

    abstract::Clear-cell carcinomas of the female genital tract are uncommon tumors that usually arise in the ovary, cervix, or vagina. The objectives of the present study were to examine the immunohistochemical and ultrastructural characteristics of 18 of these tumors and to determine whether any distinctive features were present ...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913129609016357

    authors: Kwon TJ,Ro JY,Tornos C,Ordonez NG

    更新日期:1996-11-01 00:00:00

  • Swim exercise training ameliorates hepatocyte ultrastructural alterations in rats fed on a high fat and sugar diet.

    abstract::Excessive consumption of carbohydrate and fat increases the risk of liver disease. We hypothesized that swim exercise can protect hepatocytes from ultra-structural damage induced by high cholesterol and fructose diets (HCFD). Rats were either fed with HCFD (model group) or a standard laboratory chow (control group) fo...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/01913123.2017.1422581

    authors: Dallak MA,Bin-Jaliah I,Albawardi A,Haidara MA,Sakr HF,Eid RA,Hassan WN,Al-Ani B

    更新日期:2018-03-01 00:00:00

  • Myoepithelial carcinoma arising in a benign myoepithelioma: immunohistochemical, ultrastructural, and flow-cytometrical study.

    abstract::A case of myoepithelial carcinoma arising in a benign myoepithelioma of the minor salivary gland in a 71-year-old patient is reported. The tumor presented initially on the palate and had been diagnosed as "benign lesion" 40 years before. It recurred 22, 36, and 40 years after initial presentation, and a similar histop...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章,评审

    doi:10.3109/01913129609016309

    authors: Bombí JA,Alós L,Rey MJ,Mallofré C,Cuchi A,Trasserra J,Cardesa A

    更新日期:1996-03-01 00:00:00

  • Multiple wall in-folds sub-divide single segments during capillary regression in hyperoxic acute lung injury.

    abstract::The present study provides further insight into the structural processes that remodel pulmonary capillaries in the injured adult lung. Early in hyperoxia acute lung injury (HALI), many sub-dividing segments are present throughout the capillary network before segment occlusion and loss predominate and capillary density...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913123.2014.888113

    authors: Jones RC,Capen DE

    更新日期:2014-05-01 00:00:00