Abstract:
:Abstract Diagnosis of primary ciliary dyskinesia (PCD) by identification of dynein arm loss in transmission electron microscopy (TEM) images can be confounded by high background noise due to random electron-dense material within the ciliary matrix, leading to diagnostic uncertainty even for experienced morphologists. The authors developed a novel image analysis tool to average the axonemal peripheral microtubular doublets, thereby increasing microtubular signal and reducing random background noise. In a randomized, double-blinded study that compared two experienced morphologists and three different diagnostic approaches, they found that use of this tool led to improvement in diagnostic TEM test performance.
journal_name
Ultrastruct Patholjournal_title
Ultrastructural pathologyauthors
Funkhouser WK 3rd,Niethammer M,Carson JL,Burns KA,Knowles MR,Leigh MW,Zariwala MA,Funkhouser WK Jrdoi
10.3109/01913123.2013.815081subject
Has Abstractpub_date
2014-08-01 00:00:00pages
248-55issue
4eissn
0191-3123issn
1521-0758journal_volume
38pub_type
杂志文章,随机对照试验abstract::Prolonged storage of Karnovsky's fixative, at both 4 and -20 degrees C, is possible in the diagnostic electron microscopy setting. Ultrastructural detail was not compromised in specimens processed in fixative that had been stored for 6 months. Evidence of smooth muscle and neuroendocrine differentiation was present in...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913129709021318
更新日期:1997-03-01 00:00:00
abstract::Sclerosing stromal tumors are rare, benign ovarian neoplasms of unknown etiology and histogenesis. Three sclerosing stromal tumors were evaluated by immunohistochemistry and electron microscopy and were compared to two thecomas and nonneoplastic ovarian mesenchymal tissue. The sclerosing stromal tumors and thecomas we...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913129209061364
更新日期:1992-05-01 00:00:00
abstract::We have carried out a systematic ultrastructural study of the bacilli, the cell-mediated response in the host, and the dermal microvasculature in lepromatous (LL), borderline lepromatous (BL), and borderline tuberculoid (BT) types of active leprosy (eight cases). In the types of least resistance (LL and BL), macrophag...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913129409023213
更新日期:1994-07-01 00:00:00
abstract::Intranuclear helioid bodies were identified by light microscopy in eight cases of mammary intraductal hyperplasia, only one of which was atypical. These structures appear as round, intranuclear eosinophilic bodies on light microscopic examination. When prominent, they may be and have been mistaken for viral inclusions...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913129109021889
更新日期:1991-05-01 00:00:00
abstract::To evaluate the contribution of electron microscopy to the final diagnosis of glomerulopathies, the authors established a prospective study during the first semester of 2006. A total of 52 kidney biopsies were performed with 3 samples for light microscopy, immunofluorescence, and electron microscopy. Among these renal...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913120903506637
更新日期:2010-04-01 00:00:00
abstract::Tissue biopsies and peripheral blood samples from 10 patients with the characteristic clinical features of acquired immunodeficiency syndrome (AIDS) were examined by electron microscopy and ultracytochemical myeloperoxidase technique. Abundant tubuloreticular inclusions (TRI) were detected within the endoplasmic retic...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913128309140585
更新日期:1983-06-01 00:00:00
abstract::The present study provides further insight into the structural processes that remodel pulmonary capillaries in the injured adult lung. Early in hyperoxia acute lung injury (HALI), many sub-dividing segments are present throughout the capillary network before segment occlusion and loss predominate and capillary density...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913123.2014.888113
更新日期:2014-05-01 00:00:00
abstract::Disseminated dermal tumors in a 71-year-old male represented the first clinical manifestation of a chronic myelomonocytic leukemia. The dermal infiltrate in one of the nodules consisted predominantly of closely packed pleomorphic Langerhans' cells (LC) with typical Birbeck granules and a strong reactivity for S-100 pr...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913128809058219
更新日期:1988-03-01 00:00:00
abstract::Alterations in tannic acid (TA) binding capacity of cell surface carbohydrates in normal, premalignant, and malignant squamous epithelium of the human uterine cervix have been studied using electron microscopic visualization in combination with microdensitometric evaluation. While in normal epithelium there is distinc...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913128409018586
更新日期:1984-01-01 00:00:00
abstract::A testicular tumor, removed from a 52-year-old man, was composed of uniform spindle cells and abundant interposed collagen, and was histologically diagnosed as a stromal tumor. Electron microscopy revealed cords of cells sometimes surrounded by a basal lamina. Desmosome-like junctions were found between some cells, an...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913128609007209
更新日期:1986-01-01 00:00:00
abstract::The morphological changes found in kidneys of patients undergoing chronic Cyclosporine A (CSA) treatment could, despite of numerous attempts, not yet be reproduced in reliable experimental models. The purpose of the present investigation was to systematically scan the in vivo fixed kidneys in CSA treated Wistar rats a...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913128709048438
更新日期:1987-01-01 00:00:00
abstract::Confocal microscope was used to analyze human enamel from 10 deciduous and 10 permanent teeth. Optically sectioned images were obtained. A more intense autofluorescence was found in primary teeth. This finding might be due to the greater presence of organic substances in deciduous enamel. The mean prism diameter measu...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913123.2014.960544
更新日期:2015-04-01 00:00:00
abstract::Monomorphous pituitary adenomas expressing several hormones by immunocytochemistry are common, whereas adenomas displaying multiple immunoreactivities and consisting of more than one morphologic cell types are rare. Three such unusual pituitary adenomas, surgically removed from two patients with acromegaly and one pat...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.1080/019131299281635
更新日期:1999-05-01 00:00:00
abstract::Pleomorphic liposarcoma (PL) is the least common subtype of liposarcoma, displaying a lipoblastic, malignant fibrous histiocytoma (MFH)-like and, less frequently, an epithelioid growth pattern. The epithelioid morphology in PL is still underrecognized and may closely simulate other epithelial neoplasms, mainly adrenal...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.1080/01913120290104575
更新日期:2002-09-01 00:00:00
abstract::Two cases of mixed cell type choroidal melanoma were examined by means of conventional and freeze-fracture electron microscopy. Data on average nuclear pore density were readily collected from freeze-fracture replicas of spindle A, spindle B, and epithelioid cells. Highly significant (p less than 0.001) differences in...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913129009032246
更新日期:1990-07-01 00:00:00
abstract::Helicobacter pylori is believed to cause chronic active gastritis. Infection/colonization of the gastric mucosal surface induces a mucosal inflammatory reaction in the form of lymphocytic aggregates, plasma cells and, particularly, neutrophils, which may, in turn, damage the mucosal epithelium. In vitro studies demons...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.1080/019131200750035049
更新日期:2000-09-01 00:00:00
abstract::Finger pulp biopsies of 31 patients suffering from Raynaud's syndrome (RS) were investigated by light and electron microscopy. The patients suffered from various underlying diseases. Interest was focused on the vascular changes and nerve and connective tissue alterations, especially the elastic fiber changes. In the d...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913128209016642
更新日期:1982-04-01 00:00:00
abstract::Immunogold ultrastructural localization of chromogranin A in secretory granules within tumor cells provides convincing evidence of endocrine or neuroendocrine differentiation. A modified immunogold method (designed for use on osmicated tissue) produced positive labeling of small granules not only in well-differentiate...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913129309015402
更新日期:1993-01-01 00:00:00
abstract::A case of a malignant meningioma arising in the left parapharyngeal space in a 29-year-old woman is reported. Electron microscopic examination of the tumor was critical in establishing the diagnosis. Meningiomas of the parapharyngeal space are extremely rare. This constitutes the first malignant case documented in thi...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913129609023238
更新日期:1996-01-01 00:00:00
abstract::In the last two decades, the ability of mesangial cells to respond to various stimuli or injurious agents by altering their phenotype and function has become recognized. The plasticity of these mesangial cells has been linked to the morphological and functional alterations responsible for the pathologic findings. Many...
journal_title:Ultrastructural pathology
pub_type: 杂志文章,评审
doi:10.1080/01913120600932594
更新日期:2006-11-01 00:00:00
abstract::A technique for immunoelectron microscopy is described whereby tissue is embedded in large (6 x 12 mm) plastic blocks and reembedded ("popped off") directly from semithin (3-microns) sections for thin sectioning. This technique is particularly useful in locating relatively sparse cells or tissue structures. Three acry...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913129209074550
更新日期:1992-01-01 00:00:00
abstract::Eighty-four cortical neoplasms were studied for cytokeratin and vimentin expression by immunohistochemistry and for intermediate filament aggregates by electron microscopy. Twenty oncocytomas expressed cytokeratin, 16 in a distinctive punctate pattern. These same 16 tumors also contained small globular filamentous bod...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913129109016259
更新日期:1991-07-01 00:00:00
abstract::A metastasizing carcinoid of the gastric stump found 25 years after Billroth II gastric resection for duodenal ulcer is described. Electron microscopy and optical endocrine cell staining proved the tumor to be composed of enterochromaffinlike (ECL) cells. This unusual combination further shows that, at variance with m...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913129109021888
更新日期:1991-05-01 00:00:00
abstract::The drugs cisplatin and gentamicin are used for treatment of various cancer patients suffering from infection. The authors report a detailed electron microscopic study of blood vessels in stria vascularis of guinea pigs after treatment with cisplatin alone and in combination with gentamicin. The most distinctive featu...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913129709021925
更新日期:1997-05-01 00:00:00
abstract::Abstract Metastases to the pituitary occur more frequently in patients with widespread cancer and mainly involve the posterior lobe. A few cases of metastatic carcinoma to a pituitary adenoma have been described so far. Here, the authors present an additional case of a clear cell renal cell carcinoma (CCRCC) metastati...
journal_title:Ultrastructural pathology
pub_type: 杂志文章,评审
doi:10.3109/01913123.2014.937843
更新日期:2014-12-01 00:00:00
abstract::Myeloid histocytes of dendritic cells (DCs), Langerhans cells (LCs), and macrophages in varied tissues, as leukemic blasts in acute monoblastic and monocytic leukemia (AML-M5a and M5b), are derived from monocyte progenitors in bone marrow. Based on DC induction from hematopoietic stem cells, myeloid progenitors, and m...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913123.2015.1120838
更新日期:2016-01-01 00:00:00
abstract::The ultrastructural and immunohistochemical features of a primary tumor of the ileum showing the classic histologic features of an inflammatory fibroid polyp (IFP) of the gastrointestinal tract are presented. Ultrastructurally the proliferating cells showed a combination of fibroblastic and histiocytic features, with ...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.1080/01913129009025123
更新日期:1990-03-01 00:00:00
abstract::To extend our initial observations that recombinant gamma-interferon (r gamma IFN) influences the growth and differentiation of normal cultured human keratinocytes, we studied the electron microscopic changes induced by r gamma IFN. Treatment of cultured human keratinocytes with 10(3) units/ml (7.1 nM) of r gamma IFN ...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913128609015559
更新日期:1986-01-01 00:00:00
abstract::Transmission electron microscopy examination of selected diagnostic specimens, that is, renal and nerve biopsies, can be greatly facilitated by the implementation of slot grid preparations that are inherently devoid of any grid bars. Such preparations are conducive to screening and photography at both low and intermed...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913128709048459
更新日期:1987-01-01 00:00:00
abstract::In vivo and animal models of monoclonal light chain-associated renal diseases are limited. The Vk*MYC transgenic model with multiple myeloma in 50-70 weeks old mice with renal involvement has been reported before, but detailed renal pathologic changes have not been well documented. This study fully investigated pathol...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.1080/01913123.2020.1841349
更新日期:2020-11-20 00:00:00