A new tool improves diagnostic test performance for transmission em evaluation of axonemal dynein arms.

Abstract:

:Abstract Diagnosis of primary ciliary dyskinesia (PCD) by identification of dynein arm loss in transmission electron microscopy (TEM) images can be confounded by high background noise due to random electron-dense material within the ciliary matrix, leading to diagnostic uncertainty even for experienced morphologists. The authors developed a novel image analysis tool to average the axonemal peripheral microtubular doublets, thereby increasing microtubular signal and reducing random background noise. In a randomized, double-blinded study that compared two experienced morphologists and three different diagnostic approaches, they found that use of this tool led to improvement in diagnostic TEM test performance.

journal_name

Ultrastruct Pathol

authors

Funkhouser WK 3rd,Niethammer M,Carson JL,Burns KA,Knowles MR,Leigh MW,Zariwala MA,Funkhouser WK Jr

doi

10.3109/01913123.2013.815081

subject

Has Abstract

pub_date

2014-08-01 00:00:00

pages

248-55

issue

4

eissn

0191-3123

issn

1521-0758

journal_volume

38

pub_type

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