Neutrophil fate in gingival crevicular fluid.

Abstract:

:The fate of the neutrophils within the inflammatory exudate in the periodontal crevice and their possible participation in the formation of neutrophil extracellular traps (NETs) are of clinical interest. However, the cytological analysis of clinical samples of inflammatory exudate is restricted by the obtainable quantities, which do not enable employing the routine approaches. Clinical examinations, ACLAR strip sampling, scanning electron microscopy, and confocal laser scanning microscopy were employed to analyze purulent crevicular exudate and gingival crevicular fluid in periodontitis. Bacteria, neutrophil activation, NETosis stages, and NETs were identified by molecular probe, expression of citrullinated histone H3, enzymatic digestion, and ultrastructurally. Crevicular neutrophils, all in diverse NETosis stages marked by the histone citrullination, and an abundance of NETs were found in both purulent crevicular exudate and gingival crevicular fluid. Largely varying quantities of dispersed crevicular bacteria were entrapped by NETs, but no phagocytized bacteria were evident in gingival crevicular fluid. The offered method enables for the first time the demonstration NETs in gingival crevicular fluid. The histone citrullination of all the floating crevicular neutrophils indicates that they all undergo NETosis.

journal_name

Ultrastruct Pathol

authors

Vitkov L,Klappacher M,Hannig M,Krautgartner WD

doi

10.3109/01913120903419989

subject

Has Abstract

pub_date

2010-02-01 00:00:00

pages

25-30

issue

1

eissn

0191-3123

issn

1521-0758

journal_volume

34

pub_type

杂志文章
  • Granular cell tumor: a review of the pathology and histogenesis.

    abstract::As the name suggests, the histogenesis of the granular cell tumor is uncertain, but this intriguing neoplasm has occasioned much speculation and controversy since its description in 1926 as a myoblastoma. Though ubiquitous in its anatomic location, it is usually small, solitary, benign, and readily recognized by routi...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章,评审

    doi:10.1080/019131299281545

    authors: Ordóñez NG,Mackay B

    更新日期:1999-07-01 00:00:00

  • Spectrum of low-grade fibrosarcomas: a comparative ultrastructural analysis of low-grade myxofibrosarcoma and fibromyxoid sarcoma.

    abstract::Low-grade fibrosarcomas have recently gained increasing attention in the literature, especially with the fall in popularity polls of the ubiquitous malignant fibrous histiocytoma (MFH). Firstly, most tumors previously known as myxoid MFH are labeled presently as myxofibrosarcomas. Secondly, the recognition and better ...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/019131290882259

    authors: Antonescu CR,Baren A

    更新日期:2004-09-01 00:00:00

  • Fatal pulmonary microsporidiosis due to encephalitozoon cuniculi following allogeneic bone marrow transplantation for acute myelogenous leukemia.

    abstract::Microsporidia are ubiquitous obligate eukaryotic intracellular parasites that are now felt to be more akin to degenerate fungi than to protozoa. Microsporidia can be highly pathogenic, causing a broad range of symptoms in humans, especially individuals who are immunocompromised. The vast majority of human cases of mic...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/01913120590951257

    authors: Orenstein JM,Russo P,Didier ES,Bowers C,Bunin N,Teachey DT

    更新日期:2005-05-01 00:00:00

  • Sarcomatous type of malignant mesothelioma.

    abstract::Thirteen malignant mesotheliomas of a sarcomatous type were studied by light microscopy and ten were studied by electron microscopy. The histologic patterns varied from tumor to tumor, often resembling other soft tissue sarcomas. Electron microscopic observation showed most of the tumors to be composed of primitive ce...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913128309140587

    authors: Klima M,Bossart MI

    更新日期:1983-06-01 00:00:00

  • Cytokeratin-containing globular filamentous bodies in renal oncocytoma.

    abstract::Eighty-four cortical neoplasms were studied for cytokeratin and vimentin expression by immunohistochemistry and for intermediate filament aggregates by electron microscopy. Twenty oncocytomas expressed cytokeratin, 16 in a distinctive punctate pattern. These same 16 tumors also contained small globular filamentous bod...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913129109016259

    authors: Bonsib SM,Bray C

    更新日期:1991-07-01 00:00:00

  • Intracisternal paracrystalline serpigenous inclusions: a marker of disordered fibroblastic proliferation.

    abstract::Three young children with characteristic fibroblastic tumors are presented. These comprised one case each of congenital fibrosarcoma, dermatofibrosarcoma protuberans, and fibrous hamartoma of infancy. Ultrastructural examination in each tumor revealed the presence of a peculiar intracisternal paracrystalline material ...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913128909048494

    authors: Sinclair-Smith CC,Emms M,Mills AE

    更新日期:1989-07-01 00:00:00

  • Value of transmission electron microscopy for primary ciliary dyskinesia diagnosis in the era of molecular medicine: Genetic defects with normal and non-diagnostic ciliary ultrastructure.

    abstract::Primary ciliary dyskinesia (PCD) is a genetic disorder causing chronic oto-sino-pulmonary disease. No single diagnostic test will detect all PCD cases. Transmission electron microscopy (TEM) of respiratory cilia was previously considered the gold standard diagnostic test for PCD, but 30% of all PCD cases have either n...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章,评审

    doi:10.1080/01913123.2017.1362088

    authors: Shapiro AJ,Leigh MW

    更新日期:2017-11-01 00:00:00

  • An ultrastructural study of mast cell interactions in hemangiomas.

    abstract::Hemangiomas, the most common tumors of infancy, are characterized by a postnatal period of rapid growth, followed by a phase of gradual involution. The proliferative phase is characterized by increased numbers of endothelial and mast cells and thickened basement membrane. Ultrastructural analysis of hemangiomas in the...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913128609014593

    authors: Dethlefsen SM,Mulliken JB,Glowacki J

    更新日期:1986-01-01 00:00:00

  • Monoclonal glomerulopathy with features of cryoglobulinemic glomerulopathy in murine multiple myeloma model.

    abstract::In vivo and animal models of monoclonal light chain-associated renal diseases are limited. The Vk*MYC transgenic model with multiple myeloma in 50-70 weeks old mice with renal involvement has been reported before, but detailed renal pathologic changes have not been well documented. This study fully investigated pathol...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/01913123.2020.1841349

    authors: Zhang PL,Herrera GA,Liu B

    更新日期:2020-11-20 00:00:00

  • Sclerosing stromal tumor of the ovary: an ultrastructural and immunohistochemical analysis with histogenetic considerations.

    abstract::Sclerosing stromal tumors are rare, benign ovarian neoplasms of unknown etiology and histogenesis. Three sclerosing stromal tumors were evaluated by immunohistochemistry and electron microscopy and were compared to two thecomas and nonneoplastic ovarian mesenchymal tissue. The sclerosing stromal tumors and thecomas we...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913129209061364

    authors: Shaw JA,Dabbs DJ,Geisinger KR

    更新日期:1992-05-01 00:00:00

  • Ultrastructure of the blood and lymphatic capillaries of the respiratory tissue during inflammation and endobronchial laser therapy.

    abstract::For wide application of low-energy laser irradiation in the pulmonary clinic, study of the structural basis of the therapeutic effect is necessary. The aim of this research is to describe the structural changes of the blood and lymphatic capillaries in the respiratory tissues during inflammation and following laser bi...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/01913120050132921

    authors: Polosukhin VV

    更新日期:2000-05-01 00:00:00

  • Tumor multiplicity is an independent prognostic factor of non-muscle-invasive bladder cancer treated with Bacillus Calmette-Guerin immunotherapy.

    abstract:BACKGROUND:Bacillus Calmette-Guerin (BCG) immunotherapy is regarded as the current treatment of choice for non muscle invasive bladder cancer (NMIBC), though its efficacy is limited by high recurrence and progression rate. Identification of factor prognosticators that might be helpful in discriminating between responde...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913123.2012.681833

    authors: Ajili F,Manai M,Darouiche A,Chebil M,Boubaker S

    更新日期:2012-10-01 00:00:00

  • Ultrastructural defects of the glomerular basement membranes associated with primary glomerular nephropathies.

    abstract::It is generally accepted that a glomerular basement membrane (GBM) thinner than 200 nm should be considered below normal. When this abnormality has a global and diffuse distribution, the associated clinical condition is a benign familial hematuria related to mutations of the COL4A4/COL4A3 genes, or an Alport syndrome....

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/01913120490445016

    authors: Mandache E,Gherghiceanu M

    更新日期:2004-03-01 00:00:00

  • Ultrastructural examination of renal tubular epithelial cells and hepatocytes in the course of chronic cyclosporin A treatment--a possible link to oxidative stress.

    abstract::Cyclosporin A is an immunosuppressant drug used to prevent graft rejection in organ transplantation. Side effects include toxicity to the kidney and liver. Recently, many experimental and clinical data have demonstrated that CsA-induced toxicity is associated with increased production of reactive oxygen species (ROS) ...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913123.2013.810686

    authors: Korolczuk A,Maciejewski M,Czechowska Md Phd G,Orzeł-Pankowska M

    更新日期:2013-10-01 00:00:00

  • Heterogeneity of malignant schwannomas.

    abstract::Six malignant schwannomas were studied by electron microscopy and immunohistochemistry for S-100 protein at the light and electron microscopic levels to clarify the nature of the tumor cells. Three tumors (group A) were composed of poorly differentiated tumor cells and showed no immunoreactivity for S-100 protein. One...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913128809048479

    authors: Hirose T,Sano T,Hizawa K

    更新日期:1988-01-01 00:00:00

  • Ultrastructural aspect of myocarditis: its relevance for the diagnosis.

    abstract::The Dallas consensus was used to reveal active or borderline inflammatory loci by light microscopy (LM). When lymphocyte-cardiocyte interaction was observed by electron microscopy (EM), the deleterious or dormant pattern of inflammatory process was recognized. The first was determined by lymphocytes that adhered to ca...

    journal_title:Ultrastructural pathology

    pub_type: 临床试验,杂志文章

    doi:10.3109/01913129509014620

    authors: Milin J,Stojsić D,Vucković D,Benc D,Hadzić M,Stojsić A,Zivkov-Saponja D

    更新日期:1995-11-01 00:00:00

  • Multiple wall in-folds sub-divide single segments during capillary regression in hyperoxic acute lung injury.

    abstract::The present study provides further insight into the structural processes that remodel pulmonary capillaries in the injured adult lung. Early in hyperoxia acute lung injury (HALI), many sub-dividing segments are present throughout the capillary network before segment occlusion and loss predominate and capillary density...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913123.2014.888113

    authors: Jones RC,Capen DE

    更新日期:2014-05-01 00:00:00

  • Reduplicated basal lamina in clear-cell carcinoma of the ovary: an immunohistochemical and electron microscopic study.

    abstract::Clear-cell carcinomas of the female genital tract are uncommon tumors that usually arise in the ovary, cervix, or vagina. The objectives of the present study were to examine the immunohistochemical and ultrastructural characteristics of 18 of these tumors and to determine whether any distinctive features were present ...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913129609016357

    authors: Kwon TJ,Ro JY,Tornos C,Ordonez NG

    更新日期:1996-11-01 00:00:00

  • Low magnification transmission electron microscopy in diagnostic pathology.

    abstract::Transmission electron microscopy examination of selected diagnostic specimens, that is, renal and nerve biopsies, can be greatly facilitated by the implementation of slot grid preparations that are inherently devoid of any grid bars. Such preparations are conducive to screening and photography at both low and intermed...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913128709048459

    authors: Rippstein P,Cavell S,Boivin M,Dardick I

    更新日期:1987-01-01 00:00:00

  • Immunohistochemical features of giant cell carcinoma of the lung: patterns of expression of cytokeratins, vimentin, and the mucinous glycoprotein recognized by monoclonal antibody A-80.

    abstract::Giant cell carcinoma of the lung (GCCL) is an uncommon and extremely aggressive variant of lung cancer. Characteristic microscopic findings include marked pleomorphism, aggregates of mononucleated or multinucleated giant cells (or both), a general lack of architectural cohesiveness, extensive necrosis, and endocytosis...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913129109016231

    authors: Chejfec G,Candel A,Jansson DS,Warren WH,Koukoulis GK,Gould JE,Manderino GL,Gooch GT,Gould VE

    更新日期:1991-03-01 00:00:00

  • Epithelioid variant of pleomorphic liposarcoma: a comparative immunohistochemical and ultrastructural analysis of six cases with emphasis on overlapping features with epithelial malignancies.

    abstract::Pleomorphic liposarcoma (PL) is the least common subtype of liposarcoma, displaying a lipoblastic, malignant fibrous histiocytoma (MFH)-like and, less frequently, an epithelioid growth pattern. The epithelioid morphology in PL is still underrecognized and may closely simulate other epithelial neoplasms, mainly adrenal...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/01913120290104575

    authors: Huang HY,Antonescu CR

    更新日期:2002-09-01 00:00:00

  • Ultrastructural study across the leprosy spectrum.

    abstract::We have carried out a systematic ultrastructural study of the bacilli, the cell-mediated response in the host, and the dermal microvasculature in lepromatous (LL), borderline lepromatous (BL), and borderline tuberculoid (BT) types of active leprosy (eight cases). In the types of least resistance (LL and BL), macrophag...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913129409023213

    authors: Ortega VV,Martínez Díaz F,Ortuño Pacheco G,Calderón Rubiales F

    更新日期:1994-07-01 00:00:00

  • Intracisternal tubules in human melanoma cells.

    abstract::Aggregates of parallel microtubules have been found within cisternae of the rough endoplasmic reticulum in cells of six human metastatic melanomas. The tubules have a uniform diameter and regular spacing and in longitudinal sections can be seen to possess a fine periodicity. They are compared with tubuloreticular incl...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913128009141389

    authors: Mackay B,Ayala AG

    更新日期:1980-01-01 00:00:00

  • Alpha and beta subunits of glycoprotein hormones in argyrophil pituitary tumors with small granule cells.

    abstract::A group of 33 functionless pituitary tumors with small argyrophil groups (SAG) were collected from a series of 200 pituitary adenomas (16.5% of all adenomas). Histologically, the tumors showed an unusually high frequency of trabecular patterns, perivascular pseudo-rosettes, and oncocytoid transformation. Immunoreactiv...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913128309140570

    authors: Capella C,Buffa R,Usellini L,Frigerio B,Jehenson P,Sessa F,Solcia E

    更新日期:1983-01-01 00:00:00

  • Low-grade spindle cell carcinoma of the kidney.

    abstract::The authors report a renal cell carcinoma composed largely of spindle cells of Fuhrman's nuclear grade II in which the bland appearance of the cells and low mitotic index were reminiscent of a benign or low-grade smooth muscle tumor. Keratin immunostaining was positive, but evidence of epithelial differentiation was o...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913129809032262

    authors: Lloreta J,Corominas JM,Munné A,Domínguez D,Bielsa O,Gelabert A,Serrano S

    更新日期:1998-01-01 00:00:00

  • Low-frequency noise effects on the rat parotid gland: A transmission electron microscopy study.

    abstract:INTRODUCTION:Low-frequency noise (LFN) is a ubiquitous physical stressor known to cause degenerative cellular changes and organ alterations with functional repercussions both in humans and animals. MATERIALS AND METHODS:After acceptance of the study protocol by a local ethics committee, 20 Wistar rats were randomly di...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/01913123.2017.1336188

    authors: Oliveira P,Pereira GM,Simões MS,Monteiro E,Alves de Matos AP,Águas A,Martins Dos Santos J

    更新日期:2017-07-01 00:00:00

  • Giant mitochondria with paracrystalline inclusions in paraganglioma of the urinary bladder: correlation with mitochondrial abnormalities in paragangliomas of other sites.

    abstract::Mitochondrial abnormalities have received relatively little attention in the ultrastructural evaluation of paragangliomas. Review of the few literature references dealing with this issue, however, reveals that quantitative and qualitative changes of these organelles occur in that context. A bladder paraganglioma is de...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913129409021899

    authors: Papadimitriou JC,Drachenberg CB

    更新日期:1994-11-01 00:00:00

  • Activation of monocyte-derived cells in the bone marrow of myelodysplastic syndrome.

    abstract:OBJECT:To study the relationship between monocyte/histiocyte activation and myelodysplastic syndrome (MDS). METHODS:Analyzing ultrastructure and myeloperoxidase reaction of nucleated cells in bone marrow from 59 cases of MDS by transmission electron microscopy. Four groups of MDS were subdivided on the basis of their ...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913123.2014.888116

    authors: Ru Y,Dong S,Zhang H,Zhao S,Ru K,Zheng G,Xiao Z,Eyden B

    更新日期:2014-08-01 00:00:00

  • Ultrastructural GABA immunogold labeling in the substantia nigra pars reticulata of kindled genetic absence epilepsy rats.

    abstract::Genetic Absence Epilepsy Rats from Strasbourg (GAERS) is a well-known animal model of absence epilepsy and they are resistant to electrical kindling stimulations. The present study aimed to examine possible differences in gamma-aminobutyric acid (GABA) levels and synapse counts in the substantia nigra pars reticulata ...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/01913123.2020.1839153

    authors: Sirvanci S,Akakin D,Gulcebi İdrizoglu M,Kaya OT,Karamahmutoglu T,Turgan Aşık ZN,Onat F

    更新日期:2020-11-20 00:00:00

  • Paraproteinemic crystalloidal keratopathy: an ultrastructural study of two cases, including immunoelectron microscopy.

    abstract::The ultrastructural appearances of corneal crystalloidal deposits are described in two patients with an IgG-kappa paraproteinemia of uncertain pathogenesis. The crystalloids in one patient were overwhelmingly intracellular and were found mainly in stromal keratocytes, but also in basal corneal epithelial cells and the...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章,评审

    doi:10.3109/01913129309027800

    authors: Henderson DW,Stirling JW,Lipsett J,Rozenbilds MA,Roberts-Thomson PJ,Coster DJ

    更新日期:1993-11-01 00:00:00