Abstract:
:The aim of this study was to examine the effect of the different protein supports in the cryopreservation solution on improving human ovarian tissue preservation after frozen-thawed procedures. Biopsies of ovarian cortical tissue were obtained from 14 subjects. All specimens were cryopreserved using a slow freezing/rapid thawing method in a solution consisting of propanediol and sucrose in different proportions of 3 protein supports: 30% human serum (HS) (solution A), 20% HS (solution B), or 20% fetal calf serum (solution C). After thawing, 191 follicles and a total of 70 samples were analyzed using transmission electron microscopy (TEM). The post-thaw preservation rate of the follicles in solution A was significantly higher with respect to solution C (p < 0.05). Unlike the follicles, the stromal cell morphology was not affected by any of the solutions investigated. By comparing stromal morphology and the patient age, it was found that HS better preserved the tissue in patients over 20 years of age with respect to younger ones, which showed a wider variability in ovarian preservation. TEM evaluation showed that 30% HS is more suitable for human ovarian tissue cryopreservation, and research should be focused on defining cryopreservation protocols specific to young patients.
journal_name
Ultrastruct Patholjournal_title
Ultrastructural pathologyauthors
Fabbri R,Pasquinelli G,Bracone G,Orrico C,Paradisi R,Seraccioli R,Venturoli Sdoi
10.1080/01913120600820187subject
Has Abstractpub_date
2006-07-01 00:00:00pages
253-60issue
4eissn
0191-3123issn
1521-0758pii
V87M00765Q87L357journal_volume
30pub_type
杂志文章abstract::As You Like It, Part 3, is a continuation of the lectures given by the author (Ultra Path VIII and Ultra Path IX). It is a critical historical review of topics of interest to electron microscopists, attempting to show what went wrong and perhaps also why. The topics chosen this time demonstrate the prominent role elec...
journal_title:Ultrastructural pathology
pub_type: 历史文章,杂志文章,评审
doi:10.1080/019131201300343874
更新日期:2001-05-01 00:00:00
abstract::One of the most constant and typical histological markers of thyroid papillary carcinoma is the presence of multilobated and ground-glass nuclei. With the intention of extending and characterizing the ultrastructural comprehension of this feature, thyroid papillary carcinoma cells and normal follicular cells were stud...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913129809033471
更新日期:1998-05-01 00:00:00
abstract::Liver biopsies from 16 children with clinical and pathologic evidence of chronic hepatitis have been examined by electron microscopy for cytoplasmic and nuclear changes. Parallel studies by radioimmunoassay on sera from the same patients support the diagnosis of all these cases as non-A, non-B hepatitis (NANB). Ultrat...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913128509142152
更新日期:1985-01-01 00:00:00
abstract::Pulmonary blastomas are rare neoplasms typically occurring in patients of pediatric age, clinically characterized by fever, respiratory distress, and radiologic findings of a pulmonary cystic and/or solid mass with partial or complete obliteration of emithorax. Their behavior is aggressive and outcome is poor due to f...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.1080/01913120500323456
更新日期:2005-11-01 00:00:00
abstract::Although the fine structure of extraneural sarcoidosis is well documented, ultrastructural study of central nervous system (CNS) sarcoidosis has been limited. Electron microscopic (EM) examination of 3 biopsy cases of CNS sarcoid revealed a mixed cellular population of lymphocytes, epitheloid cells, and multinucleated...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913128109048309
更新日期:1981-07-01 00:00:00
abstract::The distribution and morphology of myoepithelial cells in one case of normal breast and in 4 cases of lobular carcinoma in situ (LCIS) were studed by immunoperoxidase and immunofluorescence with anti-actin antisera and by electron microscopy. The actin-rich cells appeared to lay flattened on the basement membrane, or ...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913128109048305
更新日期:1981-07-01 00:00:00
abstract::Alterations in tannic acid (TA) binding capacity of cell surface carbohydrates in normal, premalignant, and malignant squamous epithelium of the human uterine cervix have been studied using electron microscopic visualization in combination with microdensitometric evaluation. While in normal epithelium there is distinc...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913128409018586
更新日期:1984-01-01 00:00:00
abstract::We report here ultrastructural and immunohistochemical studies of neuroblastic differentiation in the retrospective (n = 17) and prospective (n = 26) series of primitive neuroectodermal tumors (PNETs). By electron microscopy, neuritelike structures containing parallel-oriented microtubules, adhesive plaque junctions, ...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913129509064217
更新日期:1995-05-01 00:00:00
abstract::Cadmium (Cd) has been recognized as one of the most important environmental and industrial pollutants. This study investigated the impact of acute exposure to Cd on oxidative stress and the inflammatory marker interleukin-6 (IL-6) in the plasma of rats and the histological picture of liver and kidney, as well as to ex...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.1080/01913123.2018.1559566
更新日期:2018-11-01 00:00:00
abstract::In an attempt to recognize early stages of focal segmental glomerulosclerosis (FSGS) in patients with a clinical course suggesting a diagnosis other than minimal change disease (MCD) and normal histology, or minor, nondiagnostic changes on light microscopy (LM), we used a protocol for systematic and extensive electron...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913128809048472
更新日期:1988-01-01 00:00:00
abstract::Helicobacter pylori is believed to cause chronic active gastritis. Infection/colonization of the gastric mucosal surface induces a mucosal inflammatory reaction in the form of lymphocytic aggregates, plasma cells and, particularly, neutrophils, which may, in turn, damage the mucosal epithelium. In vitro studies demons...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.1080/019131200750035049
更新日期:2000-09-01 00:00:00
abstract::Adult neuroblastoma is an uncommon tumor and it is rare for one to present as a primary bone lesion. We report the case of a 23-year-old man in whom the tumor initially appeared in the right scapula. Light microscopic, immunocytochemical, and electron microscopic findings were typical of neuroblastoma. ...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913128709048440
更新日期:1987-01-01 00:00:00
abstract::Although there has been substantial progress in the identification of diarrheal diseases in infancy and childhood, electron microscopy may be still required for establishing diagnosis, staging, and response to therapy. This review describes severe conditions in which histopathologic examination alone cannot provide a ...
journal_title:Ultrastructural pathology
pub_type: 杂志文章,评审
doi:10.3109/01913121003648410
更新日期:2010-05-01 00:00:00
abstract::This article reviews literature on the transition from rudimentary prokaryotic life to eukaryotes. An overview of the differences between these organisms and theories of eukaryogenesis are reviewed. Various methods of investigating the transformation from prokaryotes to eukaryotes are elaborated, including the fossil,...
journal_title:Ultrastructural pathology
pub_type: 杂志文章,评审
doi:10.1080/01913120050118521
更新日期:2000-03-01 00:00:00
abstract::Weber-Christian disease (W-CD) is associated with relapsing nodular panniculitis and a variety of systemic findings. Renal parenchymal involvement has been rarely reported. The authors describe a case of nephrotic syndrome in an African-American man with a W-CD flare. The patient had chills and low-grade fever with pa...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913123.2012.736459
更新日期:2012-12-01 00:00:00
abstract::The ultrastructural appearance of an endometrial stromal nodule with prominent smooth and skeletal muscle differentiation is described. This is the first reported case of endometrial stromal nodule with a heterologous skeletal muscle component and emphasizes the value of electron microscopy and its correlation with im...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913129309027783
更新日期:1993-05-01 00:00:00
abstract::The ultrastructure of 10 myxoid liposarcomas from 9 patients is presented. Features closely resembling those of developing fetal adipose tissue were seen in all tumors. THe various cell types identified ranged from undifferentiated cells to typical multivacuolated and univacuolated lipoblasts. In addition, a secretory...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913128009141413
更新日期:1980-04-01 00:00:00
abstract::Hemangiomas, the most common tumors of infancy, are characterized by a postnatal period of rapid growth, followed by a phase of gradual involution. The proliferative phase is characterized by increased numbers of endothelial and mast cells and thickened basement membrane. Ultrastructural analysis of hemangiomas in the...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913128609014593
更新日期:1986-01-01 00:00:00
abstract::It is now apparent that light microscopy and histochemistry failed to identify correctly the nature and composition of pigments in various gastrointestinal tract melanoses. In most instances it was thought that the pigment was melanin or a melanin-like substance. Electron microscopy (EM) and electron-probe energy disp...
journal_title:Ultrastructural pathology
pub_type: 杂志文章,评审
doi:10.3109/01913129509064226
更新日期:1995-07-01 00:00:00
abstract::Cutaneous nerves showed multiple ultrastructural abnormalities in a patient with incontinentia pigmenti. Fetal nerve bundles were seen both in the dermis and entering the epidermis. A cell with some Schwann cell features was identified above the basal layer amid keratinocytes. Dermal melanophages were in close apposit...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913128809064214
更新日期:1988-01-01 00:00:00
abstract::A metastasizing carcinoid of the gastric stump found 25 years after Billroth II gastric resection for duodenal ulcer is described. Electron microscopy and optical endocrine cell staining proved the tumor to be composed of enterochromaffinlike (ECL) cells. This unusual combination further shows that, at variance with m...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913129109021888
更新日期:1991-05-01 00:00:00
abstract::A glomangioma (glomus tumor) arising in the pituitary region is described. The histologic and ultrastructural features were characteristic. The tumor was composed of epithelioid cells arranged in an organoid fashion around vascular channels and contained scattered mast cells. Electron microscopy revealed tumor cells t...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913128409141853
更新日期:1984-01-01 00:00:00
abstract::The aim of this study is to investigate the effects of betulinic acid (BA) on triple-negative breast cancer MDA-MB-231 cells and observe the ultrastructural changes. The concentration of BA required to induce apoptosis in MDA-MB-231 cells has been previously reported. In this study, a cell counting kit-8 proliferation...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.1080/01913123.2017.1383548
更新日期:2018-01-01 00:00:00
abstract::A spectrum of 24 benign and malignant nerve sheath tumors and 10 non-neural spindle-cell tumors were studied by light microscopy for the presence of glial fibrillary acidic protein (GFAP) immunoreactivity by the peroxidase-antiperoxidase (PAP) technique. In 8 cases, these results were compared to their electron micros...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913128409141487
更新日期:1984-01-01 00:00:00
abstract::Fabry disease is an X-linked recessive lysosomal storage disease caused by a deficiency of α-galactosidase A, with characteristic ultrastructural cytoplasmic myelin-like inclusions. Renal lesions are seen in male and variably in heterozygous female patients. One previous report has described Fabry disease involving a ...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.3109/01913123.2010.538136
更新日期:2011-04-01 00:00:00
abstract::Hydrogen sulfide (H2S) and other volatile sulfur compounds (VSCs) appear mainly in the oral air of patients with halitosis. It seems that VSCs are directly involved in the pathogenesis of gingival diseases. In previous studies, short-term (7 hours-4 days), high concentrations (5-400 ppm) of H2S applications on periodo...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.1080/01913123.2016.1234530
更新日期:2016-11-01 00:00:00
abstract::Three cases of adamantinomatous craniopharyngiomas were examined by light and electron microscopy and special attention was paid to the formation of ossified tissue. The tumors were composed of neoplastic epithelial cells with keratinized cell nests and fibrous connective tissue. Keratinized cell masses sometimes dire...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.1080/019131299281374
更新日期:1999-11-01 00:00:00
abstract::Monomorphous pituitary adenomas expressing several hormones by immunocytochemistry are common, whereas adenomas displaying multiple immunoreactivities and consisting of more than one morphologic cell types are rare. Three such unusual pituitary adenomas, surgically removed from two patients with acromegaly and one pat...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.1080/019131299281635
更新日期:1999-05-01 00:00:00
abstract::The clinicopathologic, immunocytochemical, and electron microscopic features of a case of meningeal melanocytoma arising in the phylum terminale are reported. Meningeal melanocytoma is an uncommon tumor that must be distinguished from metastatic or primary malignant melanoma, meningeal melanocytic nevi, pigmented meni...
journal_title:Ultrastructural pathology
pub_type: 杂志文章,评审
doi:10.3109/01913129809103356
更新日期:1998-07-01 00:00:00
abstract::Electron microscopic investigation of biopsy materials from Kaposi sarcoma (KS) skin lesions of 2 African AIDS patients occasionally revealed fungal cells within the tumor tissue. The spherical cells were surrounded by a triple-layered cell wall and were not encapsulated. The ultrastructural characteristics of the cel...
journal_title:Ultrastructural pathology
pub_type: 杂志文章
doi:10.1080/01913120500520960
更新日期:2006-05-01 00:00:00