As you like it, Part 3: A critique and historical review of calcification as seen with the electron microscope.

Abstract:

:As You Like It, Part 3, is a continuation of the lectures given by the author (Ultra Path VIII and Ultra Path IX). It is a critical historical review of topics of interest to electron microscopists, attempting to show what went wrong and perhaps also why. The topics chosen this time demonstrate the prominent role electron microscopy has played in elucidating the diverse ways in which calcification can occur. The classic concept of dystropic and metastatic calcification is now inadequate to explain all observed phenomena. The electron microscope shows that calcification occurs in many different intracellular and extracellular sites and that each has its own morphology and etiology. Thus, a new classification based on ultrastructural morphology is born, but few seem to be aware of it. The author examines the ubiquitous but not too well-known phenomenon of cell remodeling, which often, but not always, leads to calcification. Topics are presented under the following headings: (1) matrical lipidic debris, (2) spherical microparticles, (3) matrix vesicles, (4) intramitochondrial calcification, (5) intralysosomal calcification, (6) calcification of collagen, (7) calcification of elastic fibers, and (8) calcification of secretory products.

journal_name

Ultrastruct Pathol

authors

Ghadially FN

doi

10.1080/019131201300343874

subject

Has Abstract

pub_date

2001-05-01 00:00:00

pages

243-67

issue

3

eissn

0191-3123

issn

1521-0758

journal_volume

25

pub_type

历史文章,杂志文章,评审
  • Large block embedding and "pop-off" technique for immunoelectron microscopy.

    abstract::A technique for immunoelectron microscopy is described whereby tissue is embedded in large (6 x 12 mm) plastic blocks and reembedded ("popped off") directly from semithin (3-microns) sections for thin sectioning. This technique is particularly useful in locating relatively sparse cells or tissue structures. Three acry...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913129209074550

    authors: de Mesy Jensen KL,di Sant'Agnese PA

    更新日期:1992-01-01 00:00:00

  • Jaw osteonecrosis in patients treated with bisphosphonates: an ultrastructural study.

    abstract::Osteonecrosis of the jaw is a severe bone disorder traditionally associated with periodontal disease, local malignancy, chemotherapy, glucocorticoid therapy, or trauma. Recently a growing number of publications reported the occurrence of osteonecrosis of the jaw in patients undergoing treatment with bisphosphonates. T...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913121003729806

    authors: Perrotta I,Cristofaro MG,Amantea M,Russo E,De Fazio S,Zuccalà V,Conforti F,Amorosi A,Donato G,Tripepi S,Giudice M

    更新日期:2010-08-01 00:00:00

  • Beneficial effect of caffeic acid phenethyl ester (CAPE) on hepatocyte damage induced by bile duct ligation: an electron microscopic examination.

    abstract::Recently the authors have reported the potent beneficial effect of caffeic acid phenethyl ester (CAPE) on cholestatic oxidative liver injury induced by acute bile ligation in Swiss albino rats. Herein, they report the ultrastructural hepatocellular alterations induced by acute bile duct ligation and the effect of CAPE...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913121003788729

    authors: Eşrefoğlu M,Ara C

    更新日期:2010-10-01 00:00:00

  • Lithium effects on vesicular trafficking in hepatocellular carcinoma cells.

    abstract::Hepatocellular carcinoma (HCC) is one of the most commonly malignant tumors worldwide, characterized by the presence of many heterogeneous molecular cell events that contribute to tumor growth and progression. Endocytic processes are intimately involved in various pathological conditions, including cancer, since they ...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/01913123.2019.1701167

    authors: Taskaeva I,Bgatova N,Gogaeva I

    更新日期:2019-01-01 00:00:00

  • Comparison of ultrastructural features among neuroblastic tumors: maturation from neuroblastoma to ganglioneuroma.

    abstract::Neuroblastic tumors have the unique ability to differentiate and mature. This family of tumors is composed of the neuroblastoma, ganglioneuroblastoma, and ganglioneuroma. These tumors are derived from primordial neural crest cells that form the sympathetic nervous system. The purpose of this study was to characterize ...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913129509064236

    authors: Hicks MJ,Mackay B

    更新日期:1995-07-01 00:00:00

  • Ultrastructural phenotype of "intestinal-type" cells in columnar-lined esophagus.

    abstract::An intestinal-type epithelium is often present at columnar-lined esophagus, gastroesophageal junction or within the so-called short segment Barrett's esophagus, but ultrastructural study failed to detect enterocytes in columnar-lined esophagus. The authors have analyzed the intestinal aspects present in areas of colum...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/01913120252959281

    authors: Sbarbati A,Faccioli N,Ricci F,Merigo F,Benati D,Castaldini G,Cordiano C,Osculati F

    更新日期:2002-03-01 00:00:00

  • Unexpected Fabry disease in a renal allograft kidney: an underrecognized cause of poor allograft function.

    abstract::Fabry disease is an X-linked recessive lysosomal storage disease caused by a deficiency of α-galactosidase A, with characteristic ultrastructural cytoplasmic myelin-like inclusions. Renal lesions are seen in male and variably in heterozygous female patients. One previous report has described Fabry disease involving a ...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913123.2010.538136

    authors: Kochar O,Wick MR,Kerr SE,Oglesbee D,Cathro HP

    更新日期:2011-04-01 00:00:00

  • A new tool improves diagnostic test performance for transmission em evaluation of axonemal dynein arms.

    abstract::Abstract Diagnosis of primary ciliary dyskinesia (PCD) by identification of dynein arm loss in transmission electron microscopy (TEM) images can be confounded by high background noise due to random electron-dense material within the ciliary matrix, leading to diagnostic uncertainty even for experienced morphologists. ...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章,随机对照试验

    doi:10.3109/01913123.2013.815081

    authors: Funkhouser WK 3rd,Niethammer M,Carson JL,Burns KA,Knowles MR,Leigh MW,Zariwala MA,Funkhouser WK Jr

    更新日期:2014-08-01 00:00:00

  • Diagnostic electron microscopy on reembedded ("popped off") areas of large Spurr epoxy sections.

    abstract::A technique is described in which areas of large Spurr epoxy thick sections are directly popped off of glass slides by inverted Beem capsules filled with polymerized plastic. This technique allows the survey of large regions of tissue with the maintenance of tissue relationships and the selection of specific areas for...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913128409018580

    authors: di Sant'Agnese PA,De Mesy-Jensen KL

    更新日期:1984-01-01 00:00:00

  • Low-grade spindle cell carcinoma of the kidney.

    abstract::The authors report a renal cell carcinoma composed largely of spindle cells of Fuhrman's nuclear grade II in which the bland appearance of the cells and low mitotic index were reminiscent of a benign or low-grade smooth muscle tumor. Keratin immunostaining was positive, but evidence of epithelial differentiation was o...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913129809032262

    authors: Lloreta J,Corominas JM,Munné A,Domínguez D,Bielsa O,Gelabert A,Serrano S

    更新日期:1998-01-01 00:00:00

  • Malignant epithelial mesothelioma of the peritoneum: observations on a problem case.

    abstract::A 71-year-old asbestos-exposed male with symptoms suggestive of asbestosis for the previous 8 years presented with abdominal distension and ascites. Clinically, a diagnosis of mesothelioma carcinoma was made. Light microscopy of an omental biopsy failed to advance the diagnosis: The tumor was a solid, papillary, and g...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913129609016334

    authors: Eyden BP,Banik S,Harris M

    更新日期:1996-07-01 00:00:00

  • Thickening of glomerular capillary walls: a guide to differential diagnosis by electron microscopy.

    abstract::Thickening of capillary walls is a feature of many glomerular diseases. Widening of the wall may be produced by deposits and other changes affecting either subepithelial and subendothelial regions or the glomerular basement membrane itself. Careful light microscopic examination using special stains can distinguish som...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913128309140784

    authors: Seymour AE,Canny A,Spargo BH

    更新日期:1983-03-01 00:00:00

  • Genome, proteome, and metabolome: where are we going?

    abstract::Rapid advances have been made in recent years in understanding the genetic makeup of mankind. The human genome project has identified approximately 32,000 genes that occur in humans. It is now possible to perform genetic profiling with many of the genes to understand embryogenesis, growth and development, the normal s...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章,评审

    doi:

    authors: Hicks J

    更新日期:2003-09-01 00:00:00

  • Prolonged storage of fixative for electron microscopy: effects on tissue preservation for diagnostic specimens.

    abstract::Prolonged storage of Karnovsky's fixative, at both 4 and -20 degrees C, is possible in the diagnostic electron microscopy setting. Ultrastructural detail was not compromised in specimens processed in fixative that had been stored for 6 months. Evidence of smooth muscle and neuroendocrine differentiation was present in...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913129709021318

    authors: Mount SL,Schwarz JE,Taatjes DJ

    更新日期:1997-03-01 00:00:00

  • Ultrastructural GABA immunogold labeling in the substantia nigra pars reticulata of kindled genetic absence epilepsy rats.

    abstract::Genetic Absence Epilepsy Rats from Strasbourg (GAERS) is a well-known animal model of absence epilepsy and they are resistant to electrical kindling stimulations. The present study aimed to examine possible differences in gamma-aminobutyric acid (GABA) levels and synapse counts in the substantia nigra pars reticulata ...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/01913123.2020.1839153

    authors: Sirvanci S,Akakin D,Gulcebi İdrizoglu M,Kaya OT,Karamahmutoglu T,Turgan Aşık ZN,Onat F

    更新日期:2020-11-20 00:00:00

  • Malignant mesothelioma with intracytoplasmic crystalline inclusions.

    abstract::A 60-year-old female presented with a history of hoarseness, cough, chest pain, and dyspnea and a needle biopsy sample was submitted for histology. Light microscopy showed sheets of tumor cells with eosinophilic cytoplasm containing multiple crystals and eccentrically located nuclei. Electron microscopy showed large i...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/01913120601172489

    authors: Goedhals J,Beukes CA,Cooper S

    更新日期:2007-01-01 00:00:00

  • Actin cytoskeleton reorganization correlates with cofilin nuclear expression and ultrastructural changes in cho aa8 cell line after apoptosis and mitotic catastrophe induction by doxorubicin.

    abstract::The effect of doxorubicin on the expression of cofilin and actin in CHO AA8 cells was estimated by fluorescence and electron microscopy. The presence of cofilin and actin was observed particularly in the nuclei of cells by different modes after treatment by doxorubicin. Cells undergoing mitotic catastrophe expressed s...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913123.2010.548113

    authors: Grzanka D,Marszałek A,Izdebska M,Gackowska L,Andrzej Szczepanski M,Grzanka A

    更新日期:2011-05-01 00:00:00

  • Neuronal ceroid lipofuscinosis: an ultrastructural, genetic, and clinical study report.

    abstract::The term "neuronal ceroid lipofuscinosis" (NCL) describes a complex of inherited neurodegenerative conditions associated with storage of lipopigments in brain tissue. In 1989 Dyken proposed a classification of NCL based on the age, clinical symptoms, and ultrastructural aspects of the lipopigments. At the ultrastructu...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/019131201300004681

    authors: Boldrini R,Biselli R,Santorelli FM,Bosman C

    更新日期:2001-01-01 00:00:00

  • Intracisternal paracrystalline serpigenous inclusions: a marker of disordered fibroblastic proliferation.

    abstract::Three young children with characteristic fibroblastic tumors are presented. These comprised one case each of congenital fibrosarcoma, dermatofibrosarcoma protuberans, and fibrous hamartoma of infancy. Ultrastructural examination in each tumor revealed the presence of a peculiar intracisternal paracrystalline material ...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913128909048494

    authors: Sinclair-Smith CC,Emms M,Mills AE

    更新日期:1989-07-01 00:00:00

  • Alpha and beta subunits of glycoprotein hormones in argyrophil pituitary tumors with small granule cells.

    abstract::A group of 33 functionless pituitary tumors with small argyrophil groups (SAG) were collected from a series of 200 pituitary adenomas (16.5% of all adenomas). Histologically, the tumors showed an unusually high frequency of trabecular patterns, perivascular pseudo-rosettes, and oncocytoid transformation. Immunoreactiv...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913128309140570

    authors: Capella C,Buffa R,Usellini L,Frigerio B,Jehenson P,Sessa F,Solcia E

    更新日期:1983-01-01 00:00:00

  • Differences in nuclear pore density among human choroidal melanoma cell types.

    abstract::Two cases of mixed cell type choroidal melanoma were examined by means of conventional and freeze-fracture electron microscopy. Data on average nuclear pore density were readily collected from freeze-fracture replicas of spindle A, spindle B, and epithelioid cells. Highly significant (p less than 0.001) differences in...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913129009032246

    authors: Sacks-Wilner R,Freddo TF

    更新日期:1990-07-01 00:00:00

  • Intercellular junction formation between polymorphonuclear leukocytes, and between polymorphonuclear leukocytes and tumor cells.

    abstract::Morphologic evidence of intercellular junctions was found between polymorphonuclear leukocytes (PMNs) and between PMNs and tumor cells. All these junctions displayed electron-dense plaques along the plasma membranes with linear spaces in between. These plaques were finely granular and devoid of filaments. Fine granule...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913128309141835

    authors: Tang CK

    更新日期:1983-09-01 00:00:00

  • Pigments of the gastrointestinal tract: a comparison of light microscopic and electron microscopic findings.

    abstract::It is now apparent that light microscopy and histochemistry failed to identify correctly the nature and composition of pigments in various gastrointestinal tract melanoses. In most instances it was thought that the pigment was melanin or a melanin-like substance. Electron microscopy (EM) and electron-probe energy disp...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章,评审

    doi:10.3109/01913129509064226

    authors: Ghadially FN,Walley VM

    更新日期:1995-07-01 00:00:00

  • Immunogold localization of actin and cytokeratin filaments in myoepithelium of human parotid salivary gland.

    abstract::Myoepithelial cells of salivary gland are uniquely specialized cells; their function is unclear, but the considerable complement of muscle-specific actin suggests contractility is one function. By routine transmission electron microscopy myofilament visualization is variable. Some myoepithelial cells appear to have li...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913129209061547

    authors: Norberg L,Dardick I,Leung R,Burford-Mason AP,Rippstein P

    更新日期:1992-09-01 00:00:00

  • Value of electron microscopy in the diagnosis of glomerular diseases.

    abstract::To evaluate the contribution of electron microscopy to the final diagnosis of glomerulopathies, the authors established a prospective study during the first semester of 2006. A total of 52 kidney biopsies were performed with 3 samples for light microscopy, immunofluorescence, and electron microscopy. Among these renal...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913120903506637

    authors: Darouich S,Goucha RL,Jaafoura MH,Moussa FB,Zekri S,Maiz HB

    更新日期:2010-04-01 00:00:00

  • Correlation of proteinuria with podocyte foot process effacement in IgA nephropathy: an ultrastructural study.

    abstract:BACKGROUND:Proteinuria is an uncommon clinical manifestation of IgA nephropathy and is usually seen in cases with severe lesions like endocapillary proliferation. However, it is occasionally seen even with cases with mild glomerular manifestations and may even be of nephrotic range. PREDICTOR:Podocyte foot process eff...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913123.2014.960543

    authors: Tewari R,Nada R,Rayat CS,Boruah D,Dudeja P,Joshi K,Sakhuja V

    更新日期:2015-04-01 00:00:00

  • Xanthogranulomatous appendicitis--an incidental finding of localized pathology.

    abstract::The clinical, histopathological, and electron microscopic features of an unusual case of xanthogranulomatous appendicitis are reported. The patient, a 37-year-old female, presented with typical signs of acute appendicitis and the appendix appeared slightly dilated at laparatomy. The histopathological sections showed n...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.1080/019131200281291

    authors: Munichor M,Kerner H,Cohen H,Bickel A,Iancu TC

    更新日期:2000-01-01 00:00:00

  • Renal malakoplakia: report of a case with multifocal involvement.

    abstract::The authors report the light microscopic and ultrastructural features in one case of malakoplakia involving the kidney, the urinary bladder, and the skin. The kidney was excised. Lesions of the urinary bladder and the skin regressed after topical treatment with cholinergic agonists and antimicrobial drugs. This case i...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913129709016374

    authors: Lloreta J,Cañas MA,Munné A,Arumí M,Bielsa O,Gelabert A,Serrano S

    更新日期:1997-11-01 00:00:00

  • Embryonic ultrastructure as a guide in the diagnosis of tumors.

    abstract::The purpose of this article has been to illustrate the applications and limitations of comparing the ultrastructural morphology of certain poorly differentiated neoplasms with normal human embryonic tissues. The examples used--embryonal rhabdomyosarcoma, Wilms' tumor, and fibrous mesothelioma, in comparison with embry...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章

    doi:10.3109/01913128709048450

    authors: Dickersin GR

    更新日期:1987-01-01 00:00:00

  • Value of transmission electron microscopy for primary ciliary dyskinesia diagnosis in the era of molecular medicine: Genetic defects with normal and non-diagnostic ciliary ultrastructure.

    abstract::Primary ciliary dyskinesia (PCD) is a genetic disorder causing chronic oto-sino-pulmonary disease. No single diagnostic test will detect all PCD cases. Transmission electron microscopy (TEM) of respiratory cilia was previously considered the gold standard diagnostic test for PCD, but 30% of all PCD cases have either n...

    journal_title:Ultrastructural pathology

    pub_type: 杂志文章,评审

    doi:10.1080/01913123.2017.1362088

    authors: Shapiro AJ,Leigh MW

    更新日期:2017-11-01 00:00:00