Abstract：
:Ethanol consumption has long been associated with brain damage. However, the mechanism underlying this deleterious effect remains unclear. Among different hypotheses, acetaldehyde is regarded by certain authors as playing a major role in the expression of ethanol toxicity, but there are still some uncertainties about the exact nature of its implication. We therefore tried to characterize the profile of the alterations of neuronal viability and DNA integrity obtained after either a direct exposure to ethanol or to acetaldehyde. Ethanol at concentrations within the range of blood alcohol levels in intoxicated humans (< or = 100 mmol/L) induced DNA alterations without any apparent effect on cell viability. Acetaldehyde (< or = 1000 micromol/L) can also induce DNA alterations but with a different profile of the DNA cellular alterations. The comparison between the distributions of the comet tail DNA indicated that ethanol induced strong breaks (tail DNA > or = 60 a.u.) generation whereas acetaldehyde rather induced lower breaks (20 < or = tail DNA < or = 50 a.u.) formation but affecting a greater number of neurones. Acetaldehyde had thus a different genotoxic potential which may suggest a different mode of action or a different cellular target. Furthermore, when a single 100 mmol/L ethanol exposure did not lead to any loss of cell viability, the addition of an inhibitor of aldehyde dehydrogenase was followed by a significant loss in viability. In contrast, the inhibition of catalase, which suppresses acetaldehyde synthesis, led to no reduced viability in the same exposure conditions. ROS also reduced viability, but this was observed only after both cytochrome P450 stimulation and catalase inhibition. These combined results could suggest that acetaldehyde may play a significant role in the expression of ethanol toxicity in brain.
journal_name
Cell Biol Toxicoljournal_title
Cell biology and toxicologyauthors
Lamarche F,Gonthier B,Signorini N,Eysseric H,Barret Ldoi
10.1007/s10565-004-0087-9subject
Has Abstractpub_date
2004-11-01 00:00:00pages
361-74issue
6eissn
0742-2091issn
1573-6822journal_volume
20pub_type
杂志文章abstract：:Considering the recent studies that question previously reported cardio-protective effects of estrogen, there is a growing concern that endocrine disruptors may also contribute to the pathology of cardiovascular disease. PCB153 is one of the most commonly found polychlorinated biphenyls, and based on epidemiological s...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-010-9170-6
更新日期：2011-02-01 00:00:00
abstract：:Clinical tests of gene sequence, structure, and function are to predict, diagnose, monitor, and prognose human disease-specific phenomes, characters severities, durations, stages, and responses to therapy. The concept and content of gene tests for clinical application mainly include chromosome/chromatins, DNA, and RNA...
journal_title：Cell biology and toxicology
pub_type： 社论
doi：10.1007/s10565-019-09464-w
更新日期：2019-04-01 00:00:00
abstract：OBJECTIVES:LncRNAs (long noncoding RNAs) have been reported to critically regulate colorectal cancer (CRC). We prospectively investigated effects and mechanisms of lncRNA LINC00858 on regulation of CRC progression. METHODS:Expression of LINC00858 and its target were analyzed by quantitative real-time polymerase chain ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-019-09506-3
更新日期：2020-08-01 00:00:00
abstract：:Magnesium, an essential metal that is important in the normal functioning of DNA, has been shown to interact with some of the toxic heavy metals in respect to biochemical and molecular mechanisms and in altering the tumorigenic process. This study examined the influence of magnesium in combination with nickel and cadm...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00756493
更新日期：1994-04-01 00:00:00
abstract：:The nephrotoxic fungal toxins ochratoxin A (OA), ochratoxin B (OB) and citrinin (CIT) are natural contaminants of foods and feeds. While cytotoxicity assays have proven useful for establishing relative toxicity and structure function relationships within groups of fungal toxins, a drawback of in vitro bioassays is the...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1023/a:1007581606944
更新日期：1998-10-01 00:00:00
abstract：:The mutagenic activity of the pyrolysis products 2-amino-3-methyl-imidazo[4,5-f]-quinoline 2-amino-3,4-dimethylimidazo[4,5-f]-quinoline in Salmonella typhimurium TA98 using rat intestinal and renal subcellular fractions as activation systems was approximately 1 and 5 revertants per nmol, respectively. This was 1,000 t...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00117825
更新日期：1987-03-01 00:00:00
abstract：:Oxidative cell injury could be induced by different reactive oxygen species (ROS) operating in multiple pathways. The present work is focused on three different models of oxidative stress: the xanthine/xanthine oxidase system (XXO), an extracellular superoxide anion generator; tert-butylhydroperoxide (TBHP), an analog...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-009-9120-3
更新日期：2010-04-01 00:00:00
abstract：:We present a simple, noninvasive, nondestructive all-purpose method for the quantitative evaluation of functional effects of xenobiotics with cultured cells and the work station for its routine, easy implementation. At present 1 to 150 cells growing in one to six dishes can be studied in parallel or otherwise at time ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00755775
更新日期：1994-12-01 00:00:00
abstract：:S-(chloroethyl)-cysteine (CEC) and S-(1,2-dichlorovinyl)-cysteine (DCVC) have been proposed as intermediates in the metabolic transformation of the carcinogens 1,2-dichloroethane and 1,1,2-trichloroethylene. We have tested the ability of CEC and DCVC to induce DNA repair and genotoxic effects at the chromosomal level ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00117768
更新日期：1988-12-01 00:00:00
abstract：:Cerebral infarction causes severe morbidity and mortality. Most patients with cerebral infarction should take antiplatelet drugs daily, so the effects of those drugs on the regeneration of the brain need to be investigated. Aspirin and clopidogrel are the most widely used antiplatelet drugs for the prevention of ische...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-017-9412-y
更新日期：2018-06-01 00:00:00
abstract：:In a comprehensive assessment of genotoxicity, sodium fluoride was evaluated in a battery of cellular tests providing different genetic end points and biotransformation capabilities. The tests included the following: rat hepatocyte primary culture/DNA repair assay, Salmonella typhimurium histidine locus reversion assa...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00119244
更新日期：1988-06-01 00:00:00
abstract：:We compared the effects of four quaternary benzo[c]phenanthridine alkaloids--chelerythrine, chelilutine, sanguinarine, and sanguilutine--and two quaternary protoberberine alkaloids-berberine and coptisine--on the human cell line HeLa (cervix carcinoma cells) and the yeasts Saccharomyces cerevisiae and Schizosaccharomy...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1023/a:1010907231602
更新日期：2001-01-01 00:00:00
abstract：:The risk presented by mycotoxins is a toxicological problem. As the data given by physico-chemical analysis may be difficult to translate in terms of toxicity, however, especially when considering multiple contamination, we have developed a system for toxicological analysis of mycotoxins using cell cultures of differe...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00122655
更新日期：1989-06-01 00:00:00
abstract：:Cyclosporin A induces in vivo a severe nephrotoxicity characterized by a large decrease in renal hemodynamics. The aim of this study is to establish the ability of the known NO donor 3-morpholinosydnonimine (SIN-1) to prevent the cyclosporin A-induced contraction by using rat isolated glomeruli and cultured glomerular...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00438167
更新日期：1996-12-01 00:00:00
abstract：:To select an insert suitable for human umbilical vein endothelial cell (HUVEC) culture, we compared several available inserts of 0.2 to 0.45 micron porosity: Cellagen (ICN), Transwell-COL (Costar), Millicell-HA and CM (Millipore), Anopore (Nunc), Cyclopore (Falcon) in comparison with a control surface (Thermanox). The...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00438147
更新日期：1996-12-01 00:00:00
abstract：:The goal of cancer eradication has been overshadowed despite the continuous improvement in research and generation of novel cancer therapeutic drugs. One of the undeniable existing problems is drug resistance due to which the paradigm of killing all cancer cells is ineffective. Tumor microenvironment plays a crucial r...
journal_title：Cell biology and toxicology
pub_type： 杂志文章,评审
doi：10.1007/s10565-019-09461-z
更新日期：2019-10-01 00:00:00
abstract：:Sensitivity of baker's yeast to disulfiram (DSF) and hypersensitivity of a mutant devoid of Cu, Zn-superoxide dismutase to this compound is reported, demonstrating that yeast may be a simple convenient eukaryotic model to study the mechanism of DSF toxicity. DSF was found to induce oxidative stress in yeast cells demo...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-011-9200-z
更新日期：2012-02-01 00:00:00
abstract：:The mutation rates of tumor suppressor protein p53 gene (TP53) are high in lung adenocarcinoma and promote the development of acquired drug resistance. The present study evaluated the p53-dependent role in lung cancer cell sensitivity to PI3K-specific inhibitors, PI3K-associated inhibitors, PI3K-non-related inhibitors...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-020-09523-7
更新日期：2020-10-01 00:00:00
abstract：:Although methylguanine-DNA-methyltransferase (MGMT) plays an important role in resistance to temozolomide (TMZ) in glioma, 40% of gliomas with MGMT inactivation are still resistant to TMZ. The underlying mechanism is not clear. Here, we report that forkhead box M1 (FoxM1) transcriptionally activates the expression of ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-017-9381-1
更新日期：2017-12-01 00:00:00
abstract：:It has been well documented in in vitro studies that ambient airborne particulate matter (PM) with an aerodynamic diameter less than 2.5 μm (PM(2.5)) is capable of inducing oxidative stress, which plays a key role in PM(2.5)-mediated cytotoxicity. Although nuclear factor erythroid-2-related factor 2 (Nrf2) has been sh...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-013-9242-5
更新日期：2013-06-01 00:00:00
abstract：:Owing to the excellent antibacterial and antiviral activity, silver nanoparticles have a widespread use in the food and pharmaceutical industries. With the increase in the production and use of the related products, the potential hazard of silver nanoparticles has aroused public attention. The main purpose of this stu...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-020-09526-4
更新日期：2020-05-04 00:00:00
abstract：:Previous studies showed that the pesticide lindane (gamma-hexachlorocyclohexane) inhibits gap junction intercellular communication in rat myometrial cells. The present study tested the hypothesis that lindane and the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) inhibit gap junction communication in rat myo...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1023/b:cbto.0000029465.74815.62
更新日期：2004-05-01 00:00:00
abstract：:Because sodium fluoride (NaF) is widely used for prevention of dental caries, pathobiological effects of NaF were investigated on human oral mucosal fibroblasts. The results showed that NaF was cytotoxic to oral mucosal fibroblasts at concentrations of 4 mmol/L or higher. Exposure of cells to NaF for 2 h also inhibite...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1023/a:1007591426267
更新日期：1998-12-01 00:00:00
abstract：:The ubiquitin proteasome-proteolytic pathway has emerged as one of the most significant pathways in modulating protein homeostasis under both normal and disease states. The use of proteasome inhibitors (PI) has played a pivotal role in understanding protein turn over. The main objective of this work was to develop a c...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-010-9175-1
更新日期：2011-04-01 00:00:00
abstract：:In a number of adverse drug reactions leading to hepatotoxicity drug metabolism is thought to be involved by generation of reactive metabolites from nontoxic drugs. In this study, an in vitro assay was developed for measurement of the impact of metabolic activation of compound on the cytotoxicity toward a human hepati...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-007-9018-x
更新日期：2008-01-01 00:00:00
abstract：:Mechanisms of cell death induced by toxins probably involve one or more processes such as inhibition of protein synthesis and impairment of plasma membrane integrity leading to an increase in membrane permeability. Since one of the possible actions of mastoparan, a cationic tetradecapeptide from wasp venom, is to pert...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00135029
更新日期：1990-01-01 00:00:00
abstract：:[Pt(O,O'-acac)(γ-acac)(DMS)] (PtAcacDMS) is a new platinum compound showing low reactivity with nucleobases and specific reactivity with sulfur ligands intracellularly. It induces apoptosis in breast cancer cells, but appears to be less neurotoxic to the developing cerebellum than cisplatin (cisPt). The aim of this st...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-013-9252-3
更新日期：2013-10-01 00:00:00
abstract：:In order to clarify the protective mechanism of sodium molybdate against the acute toxicity of cadmium chloride in rat, the effect of in vivo sodium molybdate pretreatment on the cytotoxic action of cadmium in isolated hepatocytes was studied. The cytosolic pH of hepatocytes isolated from untreated rats immediately de...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00124071
更新日期：1991-10-01 00:00:00
abstract：:In the US alone, around 60,000 lives/year are lost to colon cancer. In order to study the mechanisms of colon carcinogenesis, in vitro model systems are required in addition to in vivo models. Towards this end, we have used the HT-29 colon cancer cells, cultured in Dulbecco's Modified Eagle Medium (DMEM), which were e...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-020-09579-5
更新日期：2021-01-07 00:00:00
abstract：:Metolachlor, a commonly used herbicide in the Midwestern USA, functions by inhibiting chlorophyll and protein synthesis in target plants. Herbicide exposure has led to detrimental effects in several organisms, affecting their growth and behavior; however, its mechanism of action in nontarget organisms is not yet clear...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-013-9256-z
更新日期：2013-08-01 00:00:00
