Mechanism of metolachlor action due to alterations in cell cycle progression.


:Metolachlor, a commonly used herbicide in the Midwestern USA, functions by inhibiting chlorophyll and protein synthesis in target plants. Herbicide exposure has led to detrimental effects in several organisms, affecting their growth and behavior; however, its mechanism of action in nontarget organisms is not yet clear. The EPA does not currently have enforceable regulations for maximal limits allowed in drinking water. Previous growth studies from our lab have demonstrated that increasing metolachlor concentrations and increasing time of exposure results in decreased growth of liver cells. The objective of this study was to elucidate a mechanism for this decrease of HepG2 cell growth after herbicide exposure. Results show that metolachlor at environmentally relevant levels (50-100 ppb) that previously led to decreased cell number does not lead to cell death by either necrosis or apoptosis. However, it was demonstrated that the levels of the retinoblastoma protein including two of its hyperphosphorylated forms are decreased in metolachlor exposed cells possibly leading to cell cycle arrest. The levels of another protein involved in cell cycle progression, p53, a mediator in the DNA damage response of cells, was not significantly altered except at the highest level of metolachlor (1,000 ppb) and after a 72-h exposure. These results suggest that the decrease in cell number after low-level metolachlor exposure is most likely due to an alteration in the cell cycle and not due to cell death in human liver cells.


Cell Biol Toxicol


Lowry DM,Greiner D,Fretheim M,Ubben M,Dhanwada KR




Has Abstract


2013-08-01 00:00:00












  • MC-LR-induced interaction between M2 macrophage and biliary epithelial cell promotes biliary epithelial cell proliferation and migration through regulating STAT3.

    abstract::Microcystin-leucine-arginine (MC-LR) was produced by toxic cyanobacteria, which has been shown to have potent hepatotoxicity. Our previous study has proved that MC-LR were able to promote intrahepatic biliary epithelial cell excessive proliferation. However, the underlying mechanism is not yet entirely clarified. Here...

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章


    authors: Yan M,Gu S,Pan C,Chen Y,Han X

    更新日期:2021-01-21 00:00:00

  • On the inhibition of camel retina acetylcholinesterase activity by cycloheximide in vitro.

    abstract::The kinetic parameters of inhibition of camel retinal acetylcholinesterase (AChE) activity by cycloheximide (CH) were investigated. For the control system, the Michaelis-Menten constant (K(m)) for the hydrolysis of acetylthiocholine iodide was found to be 0.076 mmol/L and the Vmax was 0.547 mumol/min per mg protein. I...

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章


    authors: al-Jafari AA,Kamal MA,Alhomida AS

    更新日期:1998-06-01 00:00:00

  • Cytotoxicity of sodium fluoride on human oral mucosal fibroblasts and its mechanisms.

    abstract::Because sodium fluoride (NaF) is widely used for prevention of dental caries, pathobiological effects of NaF were investigated on human oral mucosal fibroblasts. The results showed that NaF was cytotoxic to oral mucosal fibroblasts at concentrations of 4 mmol/L or higher. Exposure of cells to NaF for 2 h also inhibite...

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章


    authors: Jeng JH,Hsieh CC,Lan WH,Chang MC,Lin SK,Hahn LJ,Kuo MY

    更新日期:1998-12-01 00:00:00

  • Control of apolipoprotein E secretion by 25-hydroxycholesterol and proinflammatory cytokines in the human astrocytoma cell line CCF-STTG1.

    abstract::During the last few years the increased presence of proinflammatory cytokines and oxidation products in Alzheimer's disease (AD) has been largely described. Oxysterols, formed by hydroxylation of cholesterol, occur naturally in the brain and are increased in the serum of AD patients. Of these oxysterols, 25-hydroxycho...

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章


    authors: Gueguen Y,Bertrand P,Ferrari L,Batt AM,Siest G

    更新日期:2001-01-01 00:00:00

  • Nano-sized and micro-sized polystyrene particles affect phagocyte function.

    abstract::Adverse effect of nanoparticles may include impairment of phagocyte function. To identify the effect of nanoparticle size on uptake, cytotoxicity, chemotaxis, cytokine secretion, phagocytosis, oxidative burst, nitric oxide production and myeloperoxidase release, leukocytes isolated from human peripheral blood, monocyt...

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章


    authors: Prietl B,Meindl C,Roblegg E,Pieber TR,Lanzer G,Fröhlich E

    更新日期:2014-02-01 00:00:00

  • Effect of okadaic acid on carpet shell clam (Ruditapes decussatus) haemocytes by in vitro exposure and harmful algal bloom simulation assays.

    abstract::Okadaic acid (OA), produced by dinoflagellates during harmful algal blooms (HAB), belongs to the Diarrheic Shellfish Poisoning toxins that cause gastrointestinal symptoms in humans after consumption. In the present work, Ruditapes decussatus haemocytes were selected to evaluate the effect of OA on cell viability, enzy...

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章


    authors: Prado-Alvarez M,Flórez-Barrós F,Méndez J,Fernandez-Tajes J

    更新日期:2013-06-01 00:00:00

  • Role of undecan-2-one on ethanol-induced apoptosis in HepG2 cells.

    abstract::Based on the reduced expression of ethanol-oxidizing enzymes in human hepatocellular carcinoma (HepG2) cells, we analyzed the role of nonoxidative metabolites in ethanol-induced apoptosis in HepG2 cells. For this purpose, an analysis of volatile metabolites of ethanol using ion-mobility spectrometry and gas chromatogr...

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章


    authors: Castaneda F,Zimmermann D,Nolte J,Baumbach JI

    更新日期:2007-11-01 00:00:00

  • Multifunctional nanocomplexes for gene transfer and gene therapy.

    abstract::DNA formulated into aggregates with polycationic reagents are referred to by a variety of terms including non-viral vectors, synthetic vectors, lipoplexes, polyplexes and more recently nanoparticles. The capacity for delivery of multiple genes, genomic-sized constructs and siRNA delivery, with a diversity of possible ...

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章,评审


    authors: Hart SL

    更新日期:2010-02-01 00:00:00

  • Temozolomide induces activation of Wnt/β-catenin signaling in glioma cells via PI3K/Akt pathway: implications in glioma therapy.

    abstract::Glioblastoma (GBM) is the most aggressive type of glioma. Temozolomide (TMZ) is currently the drug of choice used for post-operative chemotherapy of GBM. However, the presence of intrinsic and acquired resistance hinders the success of chemotherapy. To understand the TMZ resistant mechanisms in glioma, we investigated...

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章


    authors: Tomar VS,Patil V,Somasundaram K

    更新日期:2020-06-01 00:00:00

  • Dehydroepiandrosterone and melatonin prevent Bacillus anthracis lethal toxin-induced TNF production in macrophages.

    abstract::The lethal toxin of Bacillus anthracis, which is composed of two separate proteinaceous exotoxins, namely protective antigen and lethal factor, is central to the pathogenesis of anthrax. Low levels of this toxin are known to induce release of cytokines such as tumor necrosis factor alpha (TNF-alpha). In the present st...

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章


    authors: Shin S,Hur GH,Kim YB,Yeon GB,Park KJ,Park YM,Lee WS

    更新日期:2000-01-01 00:00:00

  • Role of Yersinia enterocolitica heat-stable enterotoxin (Y-STa) on differential regulation of nuclear and cytosolic calcium signaling in rat intestinal epithelial cells.

    abstract::The heat-stable enterotoxin (Y-STa) produced by the pathogenic strains of Yersinia enterocolitica is a causative agent of secretory diarrhea. We have reported earlier that Y-STa-induced inositol trisphosphate-mediated cytosolic calcium rise occurs in rat intestinal epithelial cells. In the present communication, the i...

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章


    authors: Saha S,Chowdhury P,Mazumdar A,Pal A,Das P,Chakrabarti MK

    更新日期:2009-06-01 00:00:00

  • Inhibition of apoptosis by menadione on exposure to UVA.

    abstract::Quinones are widely distributed in the environment, both as natural products and as pollutants. This paper reports that one of the simplest quinones, 2-methyl-1,4-naphthoquinone (menadione), effectively inhibited apoptosis in the presence of UVA. Menadione suppressed the apoptosis induced by serum depletion and cell d...

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章


    authors: Ibuki Y,Toyooka T,Goto R

    更新日期:2006-09-01 00:00:00

  • SEW2871 attenuates ANIT-induced hepatotoxicity by protecting liver barrier function via sphingosine 1-phosphate receptor-1-mediated AMPK signaling pathway.

    abstract::Cholestatic liver injury, a group of diseases characterized with dysregulated bile acid (BA) homeostasis, was partly resulted from BA circulation disorders, which is commonly associated with the damage of hepatocyte barrier function. However, the underlying hepatocyte barrier-protective molecular mechanisms of cholest...

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章


    authors: Yang T,Wang X,Zhou Y,Yu Q,Heng C,Yang H,Yuan Z,Miao Y,Chai Y,Wu Z,Sun L,Huang X,Liu B,Jiang Z,Zhang L

    更新日期:2021-01-05 00:00:00

  • The time-dependent effect of 2,2'-dichlorodiethyl sulfide (sulfur mustard, HD, 1,1'-thiobis [2-chloroethane]) on the lymphocyte viability and the kinetics of protection by poly(ADP-ribose) polymerase inhibitors.

    abstract::2,2'-Dichlorodiethyl sulfide (sulfur mustard, HD, 1,1'-thiobis [2-chloroethane]) is a potent vesicant which can cause severe lesions to skin, lung, and eyes. Due to the high number of debilitating exposures during the Iran-Iraq war to the alkylating agent, HD, there is an increased interest in its mechanism of action ...

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章


    authors: Meier HL

    更新日期:1996-06-01 00:00:00

  • A tissue culture model for studying ethanol toxicity on embryonic heart cells.

    abstract::A tissue system in which fibroblasts and myocytes from chick embryonic hearts were separately maintained was used to study the toxicity of ethanol. To reproduce the teratogenic effects of acute, high concentrations of ethanol typical of "binge" drinking, an open tissue culture system was employed. With open cultures, ...

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章


    authors: Ni Y,Feng-Chen KC,Hsu L

    更新日期:1992-01-01 00:00:00

  • Transimmortalized proximal tubule and collecting duct cell lines derived from the kidneys of transgenic mice.

    abstract::This review summarizes the strategy of cellular immortalization based on the principle of targeted oncogenesis in transgenic mice, used to establish models of transimmortalized renal proximal tubule cells, referred to as PKSV-PCT and PKSV-PR-cells, and collecting duct principal cells, referred to as mpkCCD(cl4) cells....

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章,评审


    authors: Chassin C,Bens M,Vandewalle A

    更新日期:2007-07-01 00:00:00

  • Influence of age, sex and strain on the in vitro induction of unscheduled DNA synthesis in rat hepatocyte primary cultures.

    abstract::The activity of chemical-induced unscheduled DNA synthesis was evaluated in hepatocyte primary cultures from Fischer 344 and Sprague-Dawley rats over a period of two years. In this two-year study hepatocytes from both sexes and strains were prepared from animals 2, 8, 14, 20 and 25 months of age and UDS was measured b...

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章


    authors: Probst GS,Hill LE

    更新日期:1987-06-01 00:00:00

  • Development of structure-activity relationships (SARs) in allergic contact dermatitis.

    abstract::One of the major objectives at the end of this century is the development of "alternative" tests for the evaluation of the pharmacological and/or toxicological activity of newly developed molecules. Contact allergy is no exception to the rule and many research programs have been started to develop in vitro techniques ...

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章,评审


    authors: Lepoittevin JP

    更新日期:1999-02-01 00:00:00

  • A simple method for the quantitative evaluation of functional effects of xenobiotics with cultured cells.

    abstract::We present a simple, noninvasive, nondestructive all-purpose method for the quantitative evaluation of functional effects of xenobiotics with cultured cells and the work station for its routine, easy implementation. At present 1 to 150 cells growing in one to six dishes can be studied in parallel or otherwise at time ...

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章


    authors: Lees D,Pugnère D,Castel A,Raux O,Travo P

    更新日期:1994-12-01 00:00:00

  • Effects of four trichothecene mycotoxins on activation marker expression and cell proliferation of human lymphocytes in culture.

    abstract::Four trichothecene mycotoxins--the type A trichothecenes T2-toxin and diacetoxyscirpenol and the type B trichothecenes nivalenol and deoxynivalenol--were studied. The effects of these mycotoxins on the expression of the sequentially expressed activation markers CD69, CD25, and CD71 and on proliferation of human lympho...

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章


    authors: Johannisson A,Bjökhag B,Hansson W,Gadhasson IL,Thuvander A

    更新日期:1999-01-01 00:00:00

  • Effects of ambient air particles on nitric oxide production in macrophage cell lines.

    abstract::We assessed the in vitro toxicity of various particles on three murine macrophage cell lines, (J774A.1, WR19M.1, RAW264.7). The cells were exposed to aqueous suspensions (0-100 microg/30 mm2 well) of urban particulate matter (SRM-1648, SRM-1649, EHC-93), fine particulate matter (PM2.5), titanium dioxide (SRM-154b), an...

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章


    authors: Chauhan V,Breznan D,Goegan P,Nadeau D,Karthikeyan S,Brook JR,Vincent R

    更新日期:2004-07-01 00:00:00

  • In vitro interactions between several species of harmful algae and haemocytes of bivalve molluscs.

    abstract::Harmful algal blooms (HABs) can have both lethal and sublethal impacts on shellfish. To understand the possible roles of haemocytes in bivalve immune responses to HABs and how the algae are affected by these cells (haemocytes), in vitro tests between cultured harmful algal species and haemocytes of the northern quahog...

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章


    authors: Hégaret H,da Silva PM,Wikfors GH,Haberkorn H,Shumway SE,Soudant P

    更新日期:2011-08-01 00:00:00

  • Ascorbate and thiol antioxidants abolish sensitivity of yeast Saccharomyces cerevisiae to disulfiram.

    abstract::Sensitivity of baker's yeast to disulfiram (DSF) and hypersensitivity of a mutant devoid of Cu, Zn-superoxide dismutase to this compound is reported, demonstrating that yeast may be a simple convenient eukaryotic model to study the mechanism of DSF toxicity. DSF was found to induce oxidative stress in yeast cells demo...

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章


    authors: Kwolek-Mirek M,Zadrag-Tecza R,Bartosz G

    更新日期:2012-02-01 00:00:00

  • Comparison of the effects of melatonin and pentoxifylline on carbon tetrachloride-induced liver toxicity in mice.

    abstract::The purpose of the study was to determine whether along and in combination melatonin (MLT) and pentoxifylline (PTX) exerted beneficial effects on histopathological changes and changes in oxidant and antioxidant systems in liver caused by CCl4-induced liver toxicity in mice. Mice were randomly divided into six groups: ...

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章


    authors: Noyan T,Kömüroğlu U,Bayram I,Sekeroğlu MR

    更新日期:2006-11-01 00:00:00

  • Comparative study of the effects of ziram and disulfiram on human monocyte-derived macrophage functions and polarization: involvement of zinc.

    abstract::Ziram, a zinc dithiocarbamate is widely used worldwide as a fungicide in agriculture. In order to investigate ziram-induced changes in macrophage functions and polarization, human monocytes-derived macrophages in culture were treated with ziram at 0.01-10 μmol.L-1 for 4-24 h. To characterize zinc involvement in these ...

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章


    authors: Parny M,Bernad J,Prat M,Salon M,Aubouy A,Bonnafé E,Coste A,Pipy B,Treilhou M

    更新日期:2020-07-25 00:00:00

  • Benzophenone-3 increases metastasis potential in lung cancer cells via epithelial to mesenchymal transition.

    abstract::Exposure to compounds with cancer-potentiating effects can contribute to the progression of cancer. Herein we have discovered for the first time that benzophenone-3 (BP-3), a chemical used as sunscreen in various cosmetic products, enhances the ability of lung cancer cells to undergo metastasis. The exposure of the lu...

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章


    authors: Phiboonchaiyanan PP,Busaranon K,Ninsontia C,Chanvorachote P

    更新日期:2017-06-01 00:00:00

  • Development of an in vitro assay for the investigation of metabolism-induced drug hepatotoxicity.

    abstract::In a number of adverse drug reactions leading to hepatotoxicity drug metabolism is thought to be involved by generation of reactive metabolites from nontoxic drugs. In this study, an in vitro assay was developed for measurement of the impact of metabolic activation of compound on the cytotoxicity toward a human hepati...

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章


    authors: Otto M,Hansen SH,Dalgaard L,Dubois J,Badolo L

    更新日期:2008-01-01 00:00:00

  • Hsp70 expression in monocytes from patients with peripheral arterial disease and healthy controls: monocyte Hsp70 in PAD.

    abstract:BACKGROUND:Heat shock proteins (HSP) are induced during cellular stress. Their role is to chaperone cellular proteins giving protection from denaturation and ultimately preventing cell death. Monocytes are key cells involved in atherosclerosis and are highly responsive to HSP induction. Therefore, we wished to examine ...

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章


    authors: Madden J,Coward JC,Shearman CP,Grimble RF,Calder PC

    更新日期:2010-06-01 00:00:00

  • The cytogenetic and hepatotoxic effects of dioxin on mouse liver cells.

    abstract::The cytogenetic and hepatotoxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on mouse liver cells were investigated. Male C57BL/6J strain mice, which have TCDD receptors, were given single intraperitoneal injections of 25, 37.5, 75 and 150 micrograms of TCDD/kg body weight or corn oil carrier alone. Two-third...

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章


    authors: Brooks AL,Jordan SW,Bose KK,Smith J,Allison DC

    更新日期:1988-03-01 00:00:00

  • Cationic lipid-mediated gene transfer: analysis of cellular uptake and nuclear import of plasmid DNA.

    abstract::Cationic lipids are widely used for gene transfer in vitro and show promise as vectors for in vivo gene therapy applications. However, there is limited understanding of the cellular mechanisms involved in nonviral gene transfer. We investigated two major steps that could be limiting barriers to cationic lipid-mediated...

    journal_title:Cell biology and toxicology

    pub_type: 杂志文章


    authors: Escriou V,Ciolina C,Helbling-Leclerc A,Wils P,Scherman D

    更新日期:1998-03-01 00:00:00