Abstract：
:Because sodium fluoride (NaF) is widely used for prevention of dental caries, pathobiological effects of NaF were investigated on human oral mucosal fibroblasts. The results showed that NaF was cytotoxic to oral mucosal fibroblasts at concentrations of 4 mmol/L or higher. Exposure of cells to NaF for 2 h also inhibited protein synthesis, cellular ATP level and functional mitochondrial activities in a dose-dependent manner. However, incubation of cells with NaF up to 12 mmol/L for 2 h depleted only 13% of cellular glutathione level. The IC50 of NaF on cellular ATP level was about 5.75 mmol/L. Preincubation of the cells with pyruvate and succinate did not protect cells from NaF-induced ATP depletion. At concentrations of 4 mmol/L, 8 mmol/L and 12 mmol/L, NaF inhibited 31%, 56% and 57% of mitochondrial functions, respectively, after 2 h incubation. No significant inhibition for NaF was found at concentrations lower than 2 mmol/L (40 ppm). These results indicate that NaF can be toxic to oral mucosal fibroblasts in vitro by its inhibition of protein synthesis, mitochondrial function and depletion of cellular ATP. Because of repeated and long-term usage of NaF, more detailed studies should be undertaken to understand its toxic effects in vitro and in vivo.
journal_name
Cell Biol Toxicoljournal_title
Cell biology and toxicologyauthors
Jeng JH,Hsieh CC,Lan WH,Chang MC,Lin SK,Hahn LJ,Kuo MYdoi
10.1023/a:1007591426267subject
Has Abstractpub_date
1998-12-01 00:00:00pages
383-9issue
6eissn
0742-2091issn
1573-6822journal_volume
14pub_type
杂志文章abstract：:Echinoderms are valuable test species in marine ecotoxicology and offer a wide range of biological processes appropriate for this approach. Regenerating echinoderms can be regarded as amenable experimental models for testing the effects of exposure to contaminants, particularly endocrine disrupter compounds (EDCs). As...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-008-9057-y
更新日期：2008-12-01 00:00:00
abstract：:Nickel is a toxic and carcinogenic environmental and occupational pollutant and quercetin is a dietary flavonoid that is reported to modulate effects of many mutagens and carcinogens. We investigated the ability of nickel chloride to induce DNA damage in human colonic mucosa cells in the presence of quercetin, using t...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1023/a:1016059112829
更新日期：2002-01-01 00:00:00
abstract：:In a number of adverse drug reactions leading to hepatotoxicity drug metabolism is thought to be involved by generation of reactive metabolites from nontoxic drugs. In this study, an in vitro assay was developed for measurement of the impact of metabolic activation of compound on the cytotoxicity toward a human hepati...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-007-9018-x
更新日期：2008-01-01 00:00:00
abstract：:The effects of aplysiatoxin and debromoaplysiatoxin on the clonal growth rate, cross-linked envelope formation and plasminogen activator secretion of normal human bronchial epithelial cells were studied. Neither compound was mitogenic over a wide range of concentrations (10(-13) to 10(-7)M). Both aplysiatoxin and debr...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00125571
更新日期：1984-10-01 00:00:00
abstract：:Four trichothecene mycotoxins--the type A trichothecenes T2-toxin and diacetoxyscirpenol and the type B trichothecenes nivalenol and deoxynivalenol--were studied. The effects of these mycotoxins on the expression of the sequentially expressed activation markers CD69, CD25, and CD71 and on proliferation of human lympho...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1023/a:1007602011366
更新日期：1999-01-01 00:00:00
abstract：:We compared the effects of four quaternary benzo[c]phenanthridine alkaloids--chelerythrine, chelilutine, sanguinarine, and sanguilutine--and two quaternary protoberberine alkaloids-berberine and coptisine--on the human cell line HeLa (cervix carcinoma cells) and the yeasts Saccharomyces cerevisiae and Schizosaccharomy...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1023/a:1010907231602
更新日期：2001-01-01 00:00:00
abstract：:Hepatocytes have the ability to go through specialized cell cycles, which, during normal developmental liver growth, result in the formation of binuclear and polyploid cells. In the adult rat liver, the majority of the hepatocytes (about 70%) are tetraploid, 15-20% are octoploid, and only 10-15% are diploid (about 50%...
journal_title：Cell biology and toxicology
pub_type： 杂志文章,评审
doi：10.1023/a:1007487425047
更新日期：1997-07-01 00:00:00
abstract：:An in vitro human nasal model was developed as a tool to study the local tolerability of nasal powder forms using excised nasal mucosa in a diffusion chamber. The suitability of this model was tested using Sandostatin (SMS) an octapeptide analog of somatostatin, as a reference drug enhanced by Avicel (microcrystalline...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00757627
更新日期：1995-10-01 00:00:00
abstract：:The use of normal adult liver hepatocytes in cell culture for biochemical, toxicological and pharmacological studies has been greatly limited owing to the loss of replicative capacity and differentiated liver function. This is contrary to the ability of the liver to regenerate following injury in vivo. This suggests t...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00754467
更新日期：1993-10-01 00:00:00
abstract：:Recent studies have demonstrated that the insecticide DDT is a tumor promoting agent. Similar to many other tumor promoting agents, DDT has been shown to inhibit gap junctional intercellular communication (GJIC) between cells in culture, and it has been suggested that DDT-induced loss of communication between adjacent...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00249596
更新日期：1990-04-01 00:00:00
abstract：:A method for the primary culture of rat liver cells on collagen-coated dextran microcarriers is described. Ethoxycoumarin deethylase (EOD) activity 24 hr after inoculation was comparable for liver cells cultured on microcarriers and on collagen-coated dishes. Cells were cultured on microcarriers for up to 48 hr and re...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00124073
更新日期：1991-10-01 00:00:00
abstract：:Although the interactions of sulfur mustard (HD) with nucleic acids and proteins have been well studied, the toxic interactions with the membrane matrix and specially the phospholipid bilayer have so far been poorly investigated. We have used several NMR techniques to study these interactions: 1H NMR to observe the lo...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1023/a:1020815723009
更新日期：2002-01-01 00:00:00
abstract：:Cholestatic liver injury, a group of diseases characterized with dysregulated bile acid (BA) homeostasis, was partly resulted from BA circulation disorders, which is commonly associated with the damage of hepatocyte barrier function. However, the underlying hepatocyte barrier-protective molecular mechanisms of cholest...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-020-09567-9
更新日期：2021-01-05 00:00:00
abstract：:The study investigates the effect of fenugreek seed polyphenol extract (FPEt) on ethanol-induced damage in rat liver. Chronic ethanol administration (6 g kg(-1) day(-1) x 60 days) caused liver damage that was manifested by excessive formation of thiobarbituric-acid-reactive substances, lipid hydroperoxides, and conjug...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-007-9050-x
更新日期：2008-10-01 00:00:00
abstract：:This study was implemented to test the Episkin model of reconstructed epidermis in the evaluation of the efficacy of cosmetic or dermopharmaceutical products on cutaneous energy metabolism. The energy metabolism is evaluated by measuring the concentration of intracellular ATP by a method using an ultrasensitive biolum...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00755786
更新日期：1994-12-01 00:00:00
abstract：:DNA formulated into aggregates with polycationic reagents are referred to by a variety of terms including non-viral vectors, synthetic vectors, lipoplexes, polyplexes and more recently nanoparticles. The capacity for delivery of multiple genes, genomic-sized constructs and siRNA delivery, with a diversity of possible ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章,评审
doi：10.1007/s10565-009-9141-y
更新日期：2010-02-01 00:00:00
abstract：:The histopathological response and cell culture characteristics of liver cells from the R16 (grc-) strain of rats, which carries an MHC-linked deletion, were examined one week after a single intraperitoneal injection of 200 mg/kg body weight diethylnitrosamine (DEN) and were compared with the response of liver cells f...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00249594
更新日期：1990-04-01 00:00:00
abstract：:Skin intoxication by bis(beta-chloroethyl)sulfide (BCES; sulfur mustard) induces cutaneous lesions similar to thermal burns, characterized by slowness of skin healing. We have developed an in vitro model of skin equivalent to investigate mechanisms involved in this delay. Direct intoxication of dermal equivalent produ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1023/a:1007491703271
更新日期：1998-02-01 00:00:00
abstract：:Considering the recent studies that question previously reported cardio-protective effects of estrogen, there is a growing concern that endocrine disruptors may also contribute to the pathology of cardiovascular disease. PCB153 is one of the most commonly found polychlorinated biphenyls, and based on epidemiological s...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-010-9170-6
更新日期：2011-02-01 00:00:00
abstract：:The mesothelial cells (MCs) play an important role in the morpho-functional alterations of the peritoneal membrane (PM) undergoing peritoneal dialysis (PD). MCs, through the epithelial-mesenchymal transition process (EMT), progressively acquire a myofibroblast-like phenotype, promoting peritoneal fibrosis (PF) and fai...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-018-9438-9
更新日期：2019-04-01 00:00:00
abstract：:Adverse effect of nanoparticles may include impairment of phagocyte function. To identify the effect of nanoparticle size on uptake, cytotoxicity, chemotaxis, cytokine secretion, phagocytosis, oxidative burst, nitric oxide production and myeloperoxidase release, leukocytes isolated from human peripheral blood, monocyt...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-013-9265-y
更新日期：2014-02-01 00:00:00
abstract：:In order to clarify the protective mechanism of sodium molybdate against the acute toxicity of cadmium chloride in rat, the effect of in vivo sodium molybdate pretreatment on the cytotoxic action of cadmium in isolated hepatocytes was studied. The cytosolic pH of hepatocytes isolated from untreated rats immediately de...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00124071
更新日期：1991-10-01 00:00:00
abstract：OBJECTIVES:LncRNAs (long noncoding RNAs) have been reported to critically regulate colorectal cancer (CRC). We prospectively investigated effects and mechanisms of lncRNA LINC00858 on regulation of CRC progression. METHODS:Expression of LINC00858 and its target were analyzed by quantitative real-time polymerase chain ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-019-09506-3
更新日期：2020-08-01 00:00:00
abstract：:Although methylguanine-DNA-methyltransferase (MGMT) plays an important role in resistance to temozolomide (TMZ) in glioma, 40% of gliomas with MGMT inactivation are still resistant to TMZ. The underlying mechanism is not clear. Here, we report that forkhead box M1 (FoxM1) transcriptionally activates the expression of ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-017-9381-1
更新日期：2017-12-01 00:00:00
abstract：:Acute exacerbation of chronic obstructive pulmonary disease (AECOPD) as a serious event has high mortality and medical costs. Systemic inflammation and immune response are the major factors influencing the outcome and quality of patient with AECOPD. On basis of identification and validation of AECOPD-specific inflamma...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-017-9405-x
更新日期：2018-04-01 00:00:00
abstract：:Microfilaments and microtubules are components of the cytoskeleton which could be implicated in neoplastic transformation. We studied the effect of two hepatic tumor promoters, phenobarbital (PB) and biliverdin (BV), on microfilaments and microtubules of non-transformed (Cl3) and transformed (FV) hepatic epithelial ce...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00135024
更新日期：1990-01-01 00:00:00
abstract：:Clinical tests of gene sequence, structure, and function are to predict, diagnose, monitor, and prognose human disease-specific phenomes, characters severities, durations, stages, and responses to therapy. The concept and content of gene tests for clinical application mainly include chromosome/chromatins, DNA, and RNA...
journal_title：Cell biology and toxicology
pub_type： 社论
doi：10.1007/s10565-019-09464-w
更新日期：2019-04-01 00:00:00
abstract：:Cellular accumulation of mono(2-ethylhexyl)phthalate (MEHP) has been recently demonstrated to disturb fat cell energy metabolism; however, the underlying mechanism remained unclear. The study aimed to determine how MEHP influenced fat cell transcriptome and how the changes might contribute to bioenergetics. Because of...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-016-9380-7
更新日期：2017-12-01 00:00:00
abstract：:We have previously synthesized various diazenecarboxamides (subsequently referred to as diazenes) that were cytotoxic to several tumor cell lines. To increase their biological activity, the structure has been modified appropriately. In the present study we examined the effects of N(1)-phenyl-N(2)-(2-pyridinylmethyl)di...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-006-0023-2
更新日期：2006-01-01 00:00:00
abstract：:Sea urchin embryos and larvae represent suitable model systems on where to investigate the effects of heavy metals on development and cell viability. Here, we tested the toxic effects of low (10(-12 )M), medium (10(-9 )M), and high (10(-6 )M) cadmium chloride concentrations, mimicking unpolluted, moderately and highly...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-008-9066-x
更新日期：2008-12-01 00:00:00
