Abstract：
:Skin intoxication by bis(beta-chloroethyl)sulfide (BCES; sulfur mustard) induces cutaneous lesions similar to thermal burns, characterized by slowness of skin healing. We have developed an in vitro model of skin equivalent to investigate mechanisms involved in this delay. Direct intoxication of dermal equivalent produced dose- and time-dependent cytotoxicity. A decrease of macroscopic retraction of collagen gels was observed, parallel to the toxic concentration with, at histological level, absence of collagen fiber reorganization. Fibroblast synthesis of fibronectin was also inhibited by intoxication, as demonstrated at an immunobiochemical and immunohistochemical level. These dermal alterations were correlated with secondary modifications of epithelial maturation of nonintoxicated normal human keratinocytes. Cellular adhesion was perturbed, as visualized by a delay in expression and reorganization of basement membrane components, laminen, collagen IV, and fibronectin. Epidermal terminal differentiation was also affected, as shown by the absence of profilaggrin/filaggrin biosynthesis. We demonstrated in vitro, that direct dermal alterations secondarily induce disturbance of epithelial maturation. Taken together, these data show the fundamental role of dermal-epidermal interactions in a normal skin reconstruction. Clinical slowness of wound healing observed after cutaneous intoxication by BCES may thus be explained by direct alkylation of some structures and further disturbances of their biosynthesis.
journal_name
Cell Biol Toxicoljournal_title
Cell biology and toxicologyauthors
Gentilhomme E,Reano A,Pradel D,Bergier J,Schmitt D,Neveux Ydoi
10.1023/a:1007491703271subject
Has Abstractpub_date
1998-02-01 00:00:00pages
1-11issue
1eissn
0742-2091issn
1573-6822journal_volume
14pub_type
杂志文章abstract：:In vitro directional migration of 10T1/2 fibroblasts is partially inhibited by TPA but not by its non-promoting analogues. Other tumor promoters, e.g., phenobarbital, saccharin, and benzoylperoxide had no measurable effect when added in concentrations known to affect in vitro two-step transformation or intercellular c...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00120161
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journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1023/b:cbto.0000038461.02222.95
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journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-007-9020-3
更新日期：2008-01-01 00:00:00
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journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00148168
更新日期：1996-06-01 00:00:00
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journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00754467
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journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00757627
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journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-006-0097-x
更新日期：2007-03-01 00:00:00
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journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-011-9207-5
更新日期：2012-06-01 00:00:00
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journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-006-0089-x
更新日期：2006-09-01 00:00:00
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journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-007-9018-x
更新日期：2008-01-01 00:00:00
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journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-006-0054-8
更新日期：2006-05-01 00:00:00
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journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1023/a:1007421124793
更新日期：1998-06-01 00:00:00
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journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-009-9120-3
更新日期：2010-04-01 00:00:00
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journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1023/a:1007419810705
更新日期：1997-10-01 00:00:00
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journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00141284
更新日期：1988-03-01 00:00:00
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journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-018-09448-2
更新日期：2019-06-01 00:00:00
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journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-006-0137-6
更新日期：2007-03-01 00:00:00
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journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00122655
更新日期：1989-06-01 00:00:00
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journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00438147
更新日期：1996-12-01 00:00:00
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journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00756761
更新日期：1994-08-01 00:00:00
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journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-013-9256-z
更新日期：2013-08-01 00:00:00
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journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1023/a:1010907231602
更新日期：2001-01-01 00:00:00
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journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-013-9249-y
更新日期：2013-08-01 00:00:00
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journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00143361
更新日期：1996-04-01 00:00:00
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journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00117825
更新日期：1987-03-01 00:00:00
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journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-011-9201-y
更新日期：2012-02-01 00:00:00
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journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-020-09540-6
更新日期：2020-07-25 00:00:00
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journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-011-9199-1
更新日期：2011-12-01 00:00:00
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journal_title：Cell biology and toxicology
pub_type： 杂志文章,已发布勘误
doi：10.1007/s10565-019-09490-8
更新日期：2020-06-01 00:00:00
abstract：:One of the major objectives at the end of this century is the development of "alternative" tests for the evaluation of the pharmacological and/or toxicological activity of newly developed molecules. Contact allergy is no exception to the rule and many research programs have been started to develop in vitro techniques ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章,评审
doi：10.1023/a:1007502723275
更新日期：1999-02-01 00:00:00
