Abstract:
:Pulmonary alveolar macrophages exposed to very short chrysotile asbestos fibers present a typical cytotoxic response: extracellular releases of lactate dehydrogenase and beta-galactosidase, and a decrease in cellular ATP content. The objective of this study was to determine if nicotinamide and 3-aminobenzamide, two inhibitors of the ADP-ribosyl transferase, could modify the in vitro toxicity of chrysotile fibers. After 30 min of pre-exposure with each of the two inhibitors, pulmonary alveolar macrophage monolayers were concomitantly exposed for 18 hours to 50 micrograms of fibers. It was observed that, in a dose-effect relationship (5 to 30 mM), nicotinamide was very effective in reducing the extracellular liberation of the marker enzymes. At 30 mM, the enzyme releases in the medium had returned to control values; the restoration of cell viability was confirmed by ATP levels. Up to 5 mM 3-aminobenzamide did not provide any protection against chrysotile cytotoxicity. Nicotinic acid, a structural analogue of nicotinamide, but not an inhibitor of the ADP-ribosyl transferase, also showed no protective effect. Nicotinamide and 3-aminobenzamide increased the intracellular NAD+ pools, respectively by 350% and 250%. However, with or without additives, the chrysotile fibers caused a constant and significant decrease in NAD+ levels (40-55 pmoles). These results suggest that the inhibition of the nuclear ADP-ribosyl transferase is not the major mechanism by which nicotinamide protects pulmonary alveolar macrophages against the toxicity of chrysotile asbestos fibers.
journal_name
Cell Biol Toxicoljournal_title
Cell biology and toxicologyauthors
Nadeau D,Lane DAdoi
10.1007/BF00141284subject
Has Abstractpub_date
1988-03-01 00:00:00pages
13-30issue
1eissn
0742-2091issn
1573-6822journal_volume
4pub_type
杂志文章abstract::During the last few years the increased presence of proinflammatory cytokines and oxidation products in Alzheimer's disease (AD) has been largely described. Oxysterols, formed by hydroxylation of cholesterol, occur naturally in the brain and are increased in the serum of AD patients. Of these oxysterols, 25-hydroxycho...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1023/a:1011996515328
更新日期:2001-01-01 00:00:00
abstract::Acute exacerbation of chronic obstructive pulmonary disease (AECOPD) as a serious event has high mortality and medical costs. Systemic inflammation and immune response are the major factors influencing the outcome and quality of patient with AECOPD. On basis of identification and validation of AECOPD-specific inflamma...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-017-9405-x
更新日期:2018-04-01 00:00:00
abstract::The mutagenic activity of the pyrolysis products 2-amino-3-methyl-imidazo[4,5-f]-quinoline 2-amino-3,4-dimethylimidazo[4,5-f]-quinoline in Salmonella typhimurium TA98 using rat intestinal and renal subcellular fractions as activation systems was approximately 1 and 5 revertants per nmol, respectively. This was 1,000 t...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00117825
更新日期:1987-03-01 00:00:00
abstract::Recent findings suggest a functional interaction of the drug resistance enzyme glutathione S-transferase P (GSTP) with the transcription factor Nrf2, a master regulator of the adaptive stress response to cellular electrophiles. The effect of this interaction on the metabolism and redox of cellular thiols was investiga...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-020-09517-5
更新日期:2020-08-01 00:00:00
abstract::The mesothelial cells (MCs) play an important role in the morpho-functional alterations of the peritoneal membrane (PM) undergoing peritoneal dialysis (PD). MCs, through the epithelial-mesenchymal transition process (EMT), progressively acquire a myofibroblast-like phenotype, promoting peritoneal fibrosis (PF) and fai...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-018-9438-9
更新日期:2019-04-01 00:00:00
abstract::Transforming growth factor-beta1 (TGF-beta1) can activate mitogen-activated protein kinases (MAPKs) in many types of cells. The mechanism of this activation is not well elucidated. Here, we explore the role of TGF-beta/Smads signaling compounds in TGF-beta1-mediated activation of extracellular signal-regulated kinase ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-006-0097-x
更新日期:2007-03-01 00:00:00
abstract::The nephrotoxic fungal toxins ochratoxin A (OA), ochratoxin B (OB) and citrinin (CIT) are natural contaminants of foods and feeds. While cytotoxicity assays have proven useful for establishing relative toxicity and structure function relationships within groups of fungal toxins, a drawback of in vitro bioassays is the...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1023/a:1007581606944
更新日期:1998-10-01 00:00:00
abstract::A method for the primary culture of rat liver cells on collagen-coated dextran microcarriers is described. Ethoxycoumarin deethylase (EOD) activity 24 hr after inoculation was comparable for liver cells cultured on microcarriers and on collagen-coated dishes. Cells were cultured on microcarriers for up to 48 hr and re...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00124073
更新日期:1991-10-01 00:00:00
abstract::Uranium is a natural radioactive metallic element; its effect on the organism is cumulative, and chronic exposure to this element can induce carcinogenesis. Three cities of the Amazon region-Monte Alegre, Prainha, and Alenquer-in North Brazil, are located in one of the largest uranium mineralization areas of the world...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-010-9152-8
更新日期:2010-10-01 00:00:00
abstract::Nickel is a toxic and carcinogenic environmental and occupational pollutant and quercetin is a dietary flavonoid that is reported to modulate effects of many mutagens and carcinogens. We investigated the ability of nickel chloride to induce DNA damage in human colonic mucosa cells in the presence of quercetin, using t...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1023/a:1016059112829
更新日期:2002-01-01 00:00:00
abstract::Magnesium, an essential metal that is important in the normal functioning of DNA, has been shown to interact with some of the toxic heavy metals in respect to biochemical and molecular mechanisms and in altering the tumorigenic process. This study examined the influence of magnesium in combination with nickel and cadm...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00756493
更新日期:1994-04-01 00:00:00
abstract::We compared the effects of four quaternary benzo[c]phenanthridine alkaloids--chelerythrine, chelilutine, sanguinarine, and sanguilutine--and two quaternary protoberberine alkaloids-berberine and coptisine--on the human cell line HeLa (cervix carcinoma cells) and the yeasts Saccharomyces cerevisiae and Schizosaccharomy...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1023/a:1010907231602
更新日期:2001-01-01 00:00:00
abstract::Sensitivity of baker's yeast to disulfiram (DSF) and hypersensitivity of a mutant devoid of Cu, Zn-superoxide dismutase to this compound is reported, demonstrating that yeast may be a simple convenient eukaryotic model to study the mechanism of DSF toxicity. DSF was found to induce oxidative stress in yeast cells demo...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-011-9200-z
更新日期:2012-02-01 00:00:00
abstract::The lethal toxin of Bacillus anthracis, which is composed of two separate proteinaceous exotoxins, namely protective antigen and lethal factor, is central to the pathogenesis of anthrax. Low levels of this toxin are known to induce release of cytokines such as tumor necrosis factor alpha (TNF-alpha). In the present st...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1023/a:1007606921569
更新日期:2000-01-01 00:00:00
abstract::Mechanisms of cell death induced by toxins probably involve one or more processes such as inhibition of protein synthesis and impairment of plasma membrane integrity leading to an increase in membrane permeability. Since one of the possible actions of mastoparan, a cationic tetradecapeptide from wasp venom, is to pert...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00135029
更新日期:1990-01-01 00:00:00
abstract::Two human hepatoma cell lines, KYN-2 and Mz-Hep-1 were characterized in terms of glucuronidation capacity and inducibility of cytochrome P4501A1/1A2 and several UDP-glucuronosyltransferases (UGTs). Cytochrome P4501A1/1A2 activity was measured using 7-ethoxyresorufin and that of UGTs with 16 different substrates. The e...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00143361
更新日期:1996-04-01 00:00:00
abstract::The article Is the clinical lipidomics a potential goldmine?, written by Linlin Zhang, Xianlin Han and Xiangdong Wang, was originally published electronically on the publisher's internet portal (currently SpringerLink) on 21 July 2018 with open access. With the author(s)' decision to step. ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章,已发布勘误
doi:10.1007/s10565-019-09490-8
更新日期:2020-06-01 00:00:00
abstract::Microfilaments and microtubules are components of the cytoskeleton which could be implicated in neoplastic transformation. We studied the effect of two hepatic tumor promoters, phenobarbital (PB) and biliverdin (BV), on microfilaments and microtubules of non-transformed (Cl3) and transformed (FV) hepatic epithelial ce...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00135024
更新日期:1990-01-01 00:00:00
abstract::Cerebral infarction causes severe morbidity and mortality. Most patients with cerebral infarction should take antiplatelet drugs daily, so the effects of those drugs on the regeneration of the brain need to be investigated. Aspirin and clopidogrel are the most widely used antiplatelet drugs for the prevention of ische...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-017-9412-y
更新日期:2018-06-01 00:00:00
abstract::Galectin 3 (Gal-3) is upregulated in gastric epithelial cells as a host response to Helicobacter pylori infection. However, the significance of Gal-3 expression in H. pylori-infected cells is not well established. We analyzed Gal-3 intracellular expression, localization, and its effects in H. pylori-infected gastric e...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-016-9315-3
更新日期:2016-02-01 00:00:00
abstract::The culture of rabbit tenocytes could be a useful model in the study of the physiopathology and pharmacotoxicology of tendons. This work was undertaken to examine the in vitro behavior of tenocytes form juvenile rabbit Achilles tendons. We report observations of the morphological and biological characteristics of prim...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1023/b:cbto.0000010395.51944.2a
更新日期:1997-02-01 00:00:00
abstract::The goal of cancer eradication has been overshadowed despite the continuous improvement in research and generation of novel cancer therapeutic drugs. One of the undeniable existing problems is drug resistance due to which the paradigm of killing all cancer cells is ineffective. Tumor microenvironment plays a crucial r...
journal_title:Cell biology and toxicology
pub_type: 杂志文章,评审
doi:10.1007/s10565-019-09461-z
更新日期:2019-10-01 00:00:00
abstract::A useful approach for constructing dose-response relationships and for studying the underlying mechanisms by which a xenobiotic agent enhances airway reactivity is to measure the response of an isolated airway following ex vivo exposure to a pollutant. We have in this way determined the dose-response relationship betw...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1023/a:1019514830173
更新日期:2002-01-01 00:00:00
abstract:BACKGROUND AND AIMS:Endoplasmic reticulum (ER) stress is a growing concern for drug-induced toxicity which causes several side effects. Ritonavir, a potent HIV protease inhibitor, induces both ER and mitochondrial stress; however, the missing link between ER stress and mitochondrial damage has been unknown. In the pres...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-018-09451-7
更新日期:2019-06-01 00:00:00
abstract::An in vitro human nasal model was developed as a tool to study the local tolerability of nasal powder forms using excised nasal mucosa in a diffusion chamber. The suitability of this model was tested using Sandostatin (SMS) an octapeptide analog of somatostatin, as a reference drug enhanced by Avicel (microcrystalline...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00757627
更新日期:1995-10-01 00:00:00
abstract::Ethanol consumption has long been associated with brain damage. However, the mechanism underlying this deleterious effect remains unclear. Among different hypotheses, acetaldehyde is regarded by certain authors as playing a major role in the expression of ethanol toxicity, but there are still some uncertainties about ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-004-0087-9
更新日期:2004-11-01 00:00:00
abstract::It has been well documented in in vitro studies that ambient airborne particulate matter (PM) with an aerodynamic diameter less than 2.5 μm (PM(2.5)) is capable of inducing oxidative stress, which plays a key role in PM(2.5)-mediated cytotoxicity. Although nuclear factor erythroid-2-related factor 2 (Nrf2) has been sh...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-013-9242-5
更新日期:2013-06-01 00:00:00
abstract::2,2'-Dichlorodiethyl sulfide (sulfur mustard, HD, 1,1'-thiobis [2-chloroethane]) is a potent vesicant which can cause severe lesions to skin, lung, and eyes. Due to the high number of debilitating exposures during the Iran-Iraq war to the alkylating agent, HD, there is an increased interest in its mechanism of action ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00148168
更新日期:1996-06-01 00:00:00
abstract::Sister chromatid exchange rate was studied in 12 early diagnosed cases of nasopharyngeal carcinoma and in their paired controls. Exchange frequencies were also analyzed in 100 healthy subjects distributed in four regions of Hunan Province and correlated to nationality, age and sex. The incidence of sister chromatid ex...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00117868
更新日期:1987-09-01 00:00:00
abstract::In a number of adverse drug reactions leading to hepatotoxicity drug metabolism is thought to be involved by generation of reactive metabolites from nontoxic drugs. In this study, an in vitro assay was developed for measurement of the impact of metabolic activation of compound on the cytotoxicity toward a human hepati...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-007-9018-x
更新日期:2008-01-01 00:00:00