Abstract:
:The development of nontoxic but effective radioprotectors is needed because of the increasing risk of human exposure to ionizing radiation. We have reported that alpha-lipoic acid confers considerable radio-protective effect in mouse tissues when given prior to x-irradiation. In the present study, alpha-lipoic acid supplementation prior to x-irradiation with 4 and 6 Gy significantly inhibited the radiation-induced decline in total antioxidant capacity (TAC) of plasma. Radiation-induced decline in non-protein sulfhydryl content (NPSH) of different tissues, namely, brain, liver, spleen, kidney, and testis, was also ameliorated significantly at both 4 and 6 Gy doses. Maximal augmentation of radiation-induced protein carbonyl content was observed in spleen followed by brain, kidney, testis, and liver. Maximal protection in terms of carbonyl content was observed in spleen (116%) at 6 Gy dose, and minimal protection was found in liver (22.94%) at 4 Gy dose. Maximal increase in MDA (malondialdehyde) content was observed in brain, followed by testis, spleen, kidney, and liver. Protection by alpha-lipoic acid pretreatment in terms of MDA content was maximal in brain (51.67%) and minimal in spleen. The findings support the idea that alpha-lipoic acid is a free-radical scavenger and a potent antioxidant.
journal_name
Cell Biol Toxicoljournal_title
Cell biology and toxicologyauthors
Manda K,Ueno M,Moritake T,Anzai Kdoi
10.1007/s10565-006-0137-6subject
Has Abstractpub_date
2007-03-01 00:00:00pages
129-37issue
2eissn
0742-2091issn
1573-6822journal_volume
23pub_type
杂志文章abstract::The nephrotoxic fungal toxins ochratoxin A (OA), ochratoxin B (OB) and citrinin (CIT) are natural contaminants of foods and feeds. While cytotoxicity assays have proven useful for establishing relative toxicity and structure function relationships within groups of fungal toxins, a drawback of in vitro bioassays is the...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1023/a:1007581606944
更新日期:1998-10-01 00:00:00
abstract::Male albino rats with diabetes induced by the administration of streptozotocin (STZ) (45 mg/kg, i.v.) were treated with oral administration of diphenyl diselenide (DPDS) pre-dissolved in soya bean oil. A significant reduction in blood glucose levels was observed in STZ-induced diabetic rats treated with DPDS compared ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-008-9095-5
更新日期:2009-08-01 00:00:00
abstract::Ziram, a zinc dithiocarbamate is widely used worldwide as a fungicide in agriculture. In order to investigate ziram-induced changes in macrophage functions and polarization, human monocytes-derived macrophages in culture were treated with ziram at 0.01-10 μmol.L-1 for 4-24 h. To characterize zinc involvement in these ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-020-09540-6
更新日期:2020-07-25 00:00:00
abstract::Sister chromatid exchange rate was studied in 12 early diagnosed cases of nasopharyngeal carcinoma and in their paired controls. Exchange frequencies were also analyzed in 100 healthy subjects distributed in four regions of Hunan Province and correlated to nationality, age and sex. The incidence of sister chromatid ex...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00117868
更新日期:1987-09-01 00:00:00
abstract::Drug-induced liver injury (DILI) is a leading cause of discontinuation of new drug approval or withdrawal of marketed medicine based on safety due to organ vulnerability. The aim of this research is to investigate the potential abilities of four different in vitro cell models (L-02, HepG2, HepaRG, and hiHeps cell line...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-016-9316-2
更新日期:2016-02-01 00:00:00
abstract::The activity of chemical-induced unscheduled DNA synthesis was evaluated in hepatocyte primary cultures from Fischer 344 and Sprague-Dawley rats over a period of two years. In this two-year study hepatocytes from both sexes and strains were prepared from animals 2, 8, 14, 20 and 25 months of age and UDS was measured b...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00058451
更新日期:1987-06-01 00:00:00
abstract::The lethal toxin of Bacillus anthracis, which is composed of two separate proteinaceous exotoxins, namely protective antigen and lethal factor, is central to the pathogenesis of anthrax. Low levels of this toxin are known to induce release of cytokines such as tumor necrosis factor alpha (TNF-alpha). In the present st...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1023/a:1007606921569
更新日期:2000-01-01 00:00:00
abstract::The culture of rabbit tenocytes could be a useful model in the study of the physiopathology and pharmacotoxicology of tendons. This work was undertaken to examine the in vitro behavior of tenocytes form juvenile rabbit Achilles tendons. We report observations of the morphological and biological characteristics of prim...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1023/b:cbto.0000010395.51944.2a
更新日期:1997-02-01 00:00:00
abstract::Skin intoxication by bis(beta-chloroethyl)sulfide (BCES; sulfur mustard) induces cutaneous lesions similar to thermal burns, characterized by slowness of skin healing. We have developed an in vitro model of skin equivalent to investigate mechanisms involved in this delay. Direct intoxication of dermal equivalent produ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1023/a:1007491703271
更新日期:1998-02-01 00:00:00
abstract::Human tumor cells have properties in vitro or in surrogate hosts that are distinct from those of normal cells, such as immortality, anchorage independence, and tumor formation in nude mice. However, different cells from individual tumors may exhibit some, but not all of these features. In previous years, human tumor c...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1023/a:1007419810705
更新日期:1997-10-01 00:00:00
abstract::Asiatic acid (AA), a triterpene, is known to be cytotoxic to several tumor cell lines. AA induces dose- and time-dependent cell death in U-87 MG human glioblastoma. This cell death occurs via both apoptosis and necrosis. The effect of AA may be cell type-specific as AA-induced cell death was mainly apoptotic in colon ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-006-0104-2
更新日期:2006-11-01 00:00:00
abstract::The polyether toxin, okadaic acid, causes diarrhetic shellfish poisoning in humans. Despite extensive research into its cellular targets using rodent models, we know little about its putative effect(s) on innate immunity. We inoculated larvae of the greater wax moth, Galleria mellonella, with physiologically relevant ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-018-09448-2
更新日期:2019-06-01 00:00:00
abstract::Glioblastoma (GBM) is the most aggressive type of glioma. Temozolomide (TMZ) is currently the drug of choice used for post-operative chemotherapy of GBM. However, the presence of intrinsic and acquired resistance hinders the success of chemotherapy. To understand the TMZ resistant mechanisms in glioma, we investigated...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-019-09502-7
更新日期:2020-06-01 00:00:00
abstract::An in vitro human nasal model was developed as a tool to study the local tolerability of nasal powder forms using excised nasal mucosa in a diffusion chamber. The suitability of this model was tested using Sandostatin (SMS) an octapeptide analog of somatostatin, as a reference drug enhanced by Avicel (microcrystalline...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00757627
更新日期:1995-10-01 00:00:00
abstract::In a number of adverse drug reactions leading to hepatotoxicity drug metabolism is thought to be involved by generation of reactive metabolites from nontoxic drugs. In this study, an in vitro assay was developed for measurement of the impact of metabolic activation of compound on the cytotoxicity toward a human hepati...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-007-9018-x
更新日期:2008-01-01 00:00:00
abstract::Recent studies have demonstrated that the insecticide DDT is a tumor promoting agent. Similar to many other tumor promoting agents, DDT has been shown to inhibit gap junctional intercellular communication (GJIC) between cells in culture, and it has been suggested that DDT-induced loss of communication between adjacent...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00249596
更新日期:1990-04-01 00:00:00
abstract::Reciprocal interactions between tumor cells and endothelial cells constitute the most important stage of tumor metastasis. There is growing evidence suggesting that beta-estradiol and vitamin D modulate the progression of steroid-sensitive breast cancers. In keeping with those results, the purpose of the study reporte...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-005-0002-z
更新日期:2005-09-01 00:00:00
abstract::Inhibition of endosomal acidification disturbs insulin signaling in both liver and adipose cells. In this study we used MIN6 beta cells to determine whether bafilomycin, a potent inhibitor of the proton-translocating vacuolar ATPase, disrupts insulin signaling in islet beta cells. Pretreatment of MIN6 cells with varyi...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-006-0054-8
更新日期:2006-05-01 00:00:00
abstract::Microfilaments and microtubules are components of the cytoskeleton which could be implicated in neoplastic transformation. We studied the effect of two hepatic tumor promoters, phenobarbital (PB) and biliverdin (BV), on microfilaments and microtubules of non-transformed (Cl3) and transformed (FV) hepatic epithelial ce...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00135024
更新日期:1990-01-01 00:00:00
abstract::Autophagy is a conserved lysosomal degradation process, and abnormal autophagy has been associated with various pathological processes, e.g., neurodegeneration, cancer, and pathogen infection. Small chemical modulators of autophagy show the potential to treat autophagy-associated diseases. Diterpenoids, nature product...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-021-09580-6
更新日期:2021-01-23 00:00:00
abstract::Hepatocytes have the ability to go through specialized cell cycles, which, during normal developmental liver growth, result in the formation of binuclear and polyploid cells. In the adult rat liver, the majority of the hepatocytes (about 70%) are tetraploid, 15-20% are octoploid, and only 10-15% are diploid (about 50%...
journal_title:Cell biology and toxicology
pub_type: 杂志文章,评审
doi:10.1023/a:1007487425047
更新日期:1997-07-01 00:00:00
abstract::We compared the effects of four quaternary benzo[c]phenanthridine alkaloids--chelerythrine, chelilutine, sanguinarine, and sanguilutine--and two quaternary protoberberine alkaloids-berberine and coptisine--on the human cell line HeLa (cervix carcinoma cells) and the yeasts Saccharomyces cerevisiae and Schizosaccharomy...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1023/a:1010907231602
更新日期:2001-01-01 00:00:00
abstract::To determine the toxicological effects of complex mixtures of pesticides, we obtained data on 100 pesticide residues in common foods of central Italy. Fifteen pesticides were more regularly detected at higher levels (dithiocarbamates, benomyl/carbendazim, thiabendazole, diphenylamine, chlorthalonil, procymidone, fenar...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00754461
更新日期:1993-10-01 00:00:00
abstract::Deoxynivalenol (DON), nivalenol (NIV), T-2 toxin (T2), fumonisin B1 (FB1), zearalenone (ZEA), and moniliformin (MON) mycotoxins are common food and feed contaminants produced by Fusarium spp. However, while they are usually found to co-occur in a large range of commodities, only few data are available on mycotoxin co-...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-017-9408-7
更新日期:2018-06-01 00:00:00
abstract::New developments in mutagenic risk assessment have appeared in the past few years. New methods have been developed such as in vitro micronucleus assay for chromosomal alterations, comet assay for primary DNA damage, use of transgenic animals to detect in vivo gene mutations, and fluorescent in situ hybridization metho...
journal_title:Cell biology and toxicology
pub_type: 杂志文章,评审
doi:10.1023/a:1007697715084
更新日期:1999-01-01 00:00:00
abstract::Sensitivity of baker's yeast to disulfiram (DSF) and hypersensitivity of a mutant devoid of Cu, Zn-superoxide dismutase to this compound is reported, demonstrating that yeast may be a simple convenient eukaryotic model to study the mechanism of DSF toxicity. DSF was found to induce oxidative stress in yeast cells demo...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-011-9200-z
更新日期:2012-02-01 00:00:00
abstract::In a comprehensive assessment of genotoxicity, sodium fluoride was evaluated in a battery of cellular tests providing different genetic end points and biotransformation capabilities. The tests included the following: rat hepatocyte primary culture/DNA repair assay, Salmonella typhimurium histidine locus reversion assa...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00119244
更新日期:1988-06-01 00:00:00
abstract::Folic acid (FA) is a vitamin essential for normal cellular functions, growth, and development. Because humans cannot synthesize this micronutrient, it must be obtained from dietary sources through intestinal absorption. The intestinal tract is a major target for oxidative stress. Our aim was to investigate the effect ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-012-9228-8
更新日期:2012-12-01 00:00:00
abstract::The use of normal adult liver hepatocytes in cell culture for biochemical, toxicological and pharmacological studies has been greatly limited owing to the loss of replicative capacity and differentiated liver function. This is contrary to the ability of the liver to regenerate following injury in vivo. This suggests t...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00754467
更新日期:1993-10-01 00:00:00
abstract::Ten compounds representative of diverse classes of chemicals were evaluated for their cytotoxicity and transforming ability to human skin fibroblasts in vitro. Only five of the ten compounds were highly cytotoxic in the 0-100 micrograms/ml range and their order of cytotoxicity was: 2,5-bis(1-aziridinyl)-3,6-bis(carboe...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00249592
更新日期:1990-04-01 00:00:00