Abstract：
:The effects of the oxidizing agent t-butylhydroperoxide (t-BHP) were investigated on three human cell lines of different origin and growth features (A431 epithelial cells, ADF astrocytoma cells and U937 leukemic cells) using electron microscopy and electron paramagnetic resonance spectroscopy. The results indicate that important biophysical and ultrastructural modifications are induced in the plasma and mitochondrial membranes of these cells and that these changes can ultimately lead to cell death. In addition, the cell cytoskeleton also appears to be a target of hydroperoxide-mediated stress. In particular, all three cell types undergo cytoskeletal alterations leading to surface blebbing, a typical characteristic of cell damage. However, the timing and extent of this damage as well as that occurring at the mitochondrial and plasma membrane levels seems to be different: cells with weak (ADF) or absent (U937) cell-to-cell and cell-substrate contacts and a poorly developed cytoskeleton appear to be more susceptible than other cell types (e.g., A431) to t-BHP-mediated injury. These diverse cell susceptibilites to hydroperoxide-mediated oxidative stress could thus depend upon cell histotype-associated growth features.
journal_name
Cell Biol Toxicoljournal_title
Cell biology and toxicologyauthors
Malorni W,Rainaldi G,Rivabene R,Santini MTdoi
10.1007/BF00756761subject
Has Abstractpub_date
1994-08-01 00:00:00pages
207-18issue
4eissn
0742-2091issn
1573-6822journal_volume
10pub_type
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