Abstract：
:Clinical tests of gene sequence, structure, and function are to predict, diagnose, monitor, and prognose human disease-specific phenomes, characters severities, durations, stages, and responses to therapy. The concept and content of gene tests for clinical application mainly include chromosome/chromatins, DNA, and RNA. Structures and functions of chromosomes and chromatins vary among various durations, phases, and conditions, with the priority consideration in clinical gene tests. Sequences and functions of DNA and associated regulators are an important partial of clinical gene test. Another large group of RNA and RNA-associated factors also contribute to gene expression, regulation, and function. DNA/RNA sequencing is used to measure tumor mutation and heterogeneity, recategorize molecular phenomes and types of cancer, or guide and predict target-based therapies. The structure and function of genome dimensions and regulations as well as various factor involvement and contributions should be seriously considered in clinical gene tests, although there are a number of challenges to be overcome, e.g., method sensitivity, specificity, stability, analysis, and clinical significance. It is also critical to have the national and international standardization, guideline, and consortium of sample handling, experimental operation, quality control, data analysis, and clinical interpretation, when clinical gene tests are developed and applied for clinical application. Thus, there is an urgent need to discover and validate those gene tests according to disease phenomes, subtypes, severity, duration, phase, progression, prognosis, and response to therapy.
journal_name
Cell Biol Toxicoljournal_title
Cell biology and toxicologyauthors
Wu D,Cheng Y,Wang X,CSGT group.doi
10.1007/s10565-019-09464-wsubject
Has Abstractpub_date
2019-04-01 00:00:00pages
83-87issue
2eissn
0742-2091issn
1573-6822pii
10.1007/s10565-019-09464-wjournal_volume
35pub_type
社论abstract：:Various in vitro preparations have been utilized to study the cellular activity of vasoactive agents on renal cortical microvessels and on mesangial cells. The receptors and the transduction pathways of bradykinin and atrial natriuretic factor were characterized on cultured cortical vascular smooth muscle cells from t...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00438157
更新日期：1996-12-01 00:00:00
abstract：:We explored if epigenetic mechanisms could be involved in the down-regulated expression of catalase gene (CAT) in the doxorubicin-resistant acute myelogenous leukemia (AML)-2/DX100 cells. Down-regulated CAT expression in AML-2/DX100 cells was completely recovered after treatment of hydrogen peroxide (H(2)O(2)) and his...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-011-9201-y
更新日期：2012-02-01 00:00:00
abstract：:The mutagenic activity of the pyrolysis products 2-amino-3-methyl-imidazo[4,5-f]-quinoline 2-amino-3,4-dimethylimidazo[4,5-f]-quinoline in Salmonella typhimurium TA98 using rat intestinal and renal subcellular fractions as activation systems was approximately 1 and 5 revertants per nmol, respectively. This was 1,000 t...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00117825
更新日期：1987-03-01 00:00:00
abstract：:Folic acid (FA) is a vitamin essential for normal cellular functions, growth, and development. Because humans cannot synthesize this micronutrient, it must be obtained from dietary sources through intestinal absorption. The intestinal tract is a major target for oxidative stress. Our aim was to investigate the effect ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-012-9228-8
更新日期：2012-12-01 00:00:00
abstract：:Bisnaphthalimido compounds bis-intercalate to DNA via the major groove and are potentially potent cancer therapeutics. Previously, we incorporated natural polyamines as linkers connecting the two naphthalimido ring moieties to create a series of soluble bisnaphthalimidopropyl polyamines (BNIPPs). Here, extending earli...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-011-9199-1
更新日期：2011-12-01 00:00:00
abstract：:Galectin 3 (Gal-3) is upregulated in gastric epithelial cells as a host response to Helicobacter pylori infection. However, the significance of Gal-3 expression in H. pylori-infected cells is not well established. We analyzed Gal-3 intracellular expression, localization, and its effects in H. pylori-infected gastric e...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-016-9315-3
更新日期：2016-02-01 00:00:00
abstract：:The purpose of the study was to determine whether along and in combination melatonin (MLT) and pentoxifylline (PTX) exerted beneficial effects on histopathological changes and changes in oxidant and antioxidant systems in liver caused by CCl4-induced liver toxicity in mice. Mice were randomly divided into six groups: ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-006-0019-y
更新日期：2006-11-01 00:00:00
abstract：:Cerebral infarction causes severe morbidity and mortality. Most patients with cerebral infarction should take antiplatelet drugs daily, so the effects of those drugs on the regeneration of the brain need to be investigated. Aspirin and clopidogrel are the most widely used antiplatelet drugs for the prevention of ische...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-017-9412-y
更新日期：2018-06-01 00:00:00
abstract：:Four trichothecene mycotoxins--the type A trichothecenes T2-toxin and diacetoxyscirpenol and the type B trichothecenes nivalenol and deoxynivalenol--were studied. The effects of these mycotoxins on the expression of the sequentially expressed activation markers CD69, CD25, and CD71 and on proliferation of human lympho...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1023/a:1007602011366
更新日期：1999-01-01 00:00:00
abstract：:Oxidative cell injury could be induced by different reactive oxygen species (ROS) operating in multiple pathways. The present work is focused on three different models of oxidative stress: the xanthine/xanthine oxidase system (XXO), an extracellular superoxide anion generator; tert-butylhydroperoxide (TBHP), an analog...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-009-9120-3
更新日期：2010-04-01 00:00:00
abstract：:Many of the differentiated functions of hepatocytes are lost in culture, yet addition of certain medium supplements can aid in the retention of differentiated character. Therefore, the effect of time in monolayer culture on rat hepatocyte glutathione (GSH) synthesis and sensitivity to the GSH detoxicated xenobiotic et...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00117851
更新日期：1986-12-01 00:00:00
abstract：:RANTES is a chemokine with eosinophil attractant and activating activities. This study was undertaken to determine whether primary cultures of human nasal and primate bronchial epithelial cells produce RANTES and the effect of various cytokines and dexamethasone on the release of this chemokine. Nasal epithelial cells...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1023/a:1007318514715
更新日期：1997-03-01 00:00:00
abstract：:We present a simple, noninvasive, nondestructive all-purpose method for the quantitative evaluation of functional effects of xenobiotics with cultured cells and the work station for its routine, easy implementation. At present 1 to 150 cells growing in one to six dishes can be studied in parallel or otherwise at time ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00755775
更新日期：1994-12-01 00:00:00
abstract：OBJECTIVES:LncRNAs (long noncoding RNAs) have been reported to critically regulate colorectal cancer (CRC). We prospectively investigated effects and mechanisms of lncRNA LINC00858 on regulation of CRC progression. METHODS:Expression of LINC00858 and its target were analyzed by quantitative real-time polymerase chain ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-019-09506-3
更新日期：2020-08-01 00:00:00
abstract：:The activity of chemical-induced unscheduled DNA synthesis was evaluated in hepatocyte primary cultures from Fischer 344 and Sprague-Dawley rats over a period of two years. In this two-year study hepatocytes from both sexes and strains were prepared from animals 2, 8, 14, 20 and 25 months of age and UDS was measured b...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00058451
更新日期：1987-06-01 00:00:00
abstract：:The kinetic parameters of inhibition of camel retinal acetylcholinesterase (AChE) activity by cycloheximide (CH) were investigated. For the control system, the Michaelis-Menten constant (K(m)) for the hydrolysis of acetylthiocholine iodide was found to be 0.076 mmol/L and the Vmax was 0.547 mumol/min per mg protein. I...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1023/a:1007421124793
更新日期：1998-06-01 00:00:00
abstract：:Because sodium fluoride (NaF) is widely used for prevention of dental caries, pathobiological effects of NaF were investigated on human oral mucosal fibroblasts. The results showed that NaF was cytotoxic to oral mucosal fibroblasts at concentrations of 4 mmol/L or higher. Exposure of cells to NaF for 2 h also inhibite...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1023/a:1007591426267
更新日期：1998-12-01 00:00:00
abstract：:The risk presented by mycotoxins is a toxicological problem. As the data given by physico-chemical analysis may be difficult to translate in terms of toxicity, however, especially when considering multiple contamination, we have developed a system for toxicological analysis of mycotoxins using cell cultures of differe...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00122655
更新日期：1989-06-01 00:00:00
abstract：:It has been well documented in in vitro studies that ambient airborne particulate matter (PM) with an aerodynamic diameter less than 2.5 μm (PM(2.5)) is capable of inducing oxidative stress, which plays a key role in PM(2.5)-mediated cytotoxicity. Although nuclear factor erythroid-2-related factor 2 (Nrf2) has been sh...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-013-9242-5
更新日期：2013-06-01 00:00:00
abstract：:Considering the recent studies that question previously reported cardio-protective effects of estrogen, there is a growing concern that endocrine disruptors may also contribute to the pathology of cardiovascular disease. PCB153 is one of the most commonly found polychlorinated biphenyls, and based on epidemiological s...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-010-9170-6
更新日期：2011-02-01 00:00:00
abstract：BACKGROUND:Heat shock proteins (HSP) are induced during cellular stress. Their role is to chaperone cellular proteins giving protection from denaturation and ultimately preventing cell death. Monocytes are key cells involved in atherosclerosis and are highly responsive to HSP induction. Therefore, we wished to examine ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-009-9134-x
更新日期：2010-06-01 00:00:00
abstract：:Although methylguanine-DNA-methyltransferase (MGMT) plays an important role in resistance to temozolomide (TMZ) in glioma, 40% of gliomas with MGMT inactivation are still resistant to TMZ. The underlying mechanism is not clear. Here, we report that forkhead box M1 (FoxM1) transcriptionally activates the expression of ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-017-9381-1
更新日期：2017-12-01 00:00:00
abstract：:[Pt(O,O'-acac)(γ-acac)(DMS)] (PtAcacDMS) is a new platinum compound showing low reactivity with nucleobases and specific reactivity with sulfur ligands intracellularly. It induces apoptosis in breast cancer cells, but appears to be less neurotoxic to the developing cerebellum than cisplatin (cisPt). The aim of this st...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-013-9252-3
更新日期：2013-10-01 00:00:00
abstract：:Gap junctions (GJ) represent a cellular communication system known to influence neuronal differentiation and survival. To assess a putative role of this system for neural effects of tamoxifen (TAM) and raloxifene (RAL), we used the human teratocarcinoma cell line NTera2/D1, retinoic acid (RA)-dependent neuronal differ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-010-9165-3
更新日期：2010-12-01 00:00:00
abstract：:Knowledge of the acute toxic effects of chemicals is important for the protection of exposed humans. Since sufficient information in humans is often lacking, experiments on laboratory animals must be performed. The LD50 test, which requires large numbers of animals, has become the preferred procedure. It is now widely...
journal_title：Cell biology and toxicology
pub_type： 杂志文章,评审
doi：10.1007/BF00121848
更新日期：1986-09-01 00:00:00
abstract：:Inhibition of endosomal acidification disturbs insulin signaling in both liver and adipose cells. In this study we used MIN6 beta cells to determine whether bafilomycin, a potent inhibitor of the proton-translocating vacuolar ATPase, disrupts insulin signaling in islet beta cells. Pretreatment of MIN6 cells with varyi...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-006-0054-8
更新日期：2006-05-01 00:00:00
abstract：:Aging, injuries, and diseases can be considered as the result of malfunctioning or damaged cells. Regenerative medicine aims to restore tissue homeostasis by repairing or replacing cells, tissues, or damaged organs, by linking and combining different disciplines including engineering, technology, biology, and medicine...
journal_title：Cell biology and toxicology
pub_type： 杂志文章,评审
doi：10.1007/s10565-017-9384-y
更新日期：2017-08-01 00:00:00
abstract：:Sea urchin embryos and larvae represent suitable model systems on where to investigate the effects of heavy metals on development and cell viability. Here, we tested the toxic effects of low (10(-12 )M), medium (10(-9 )M), and high (10(-6 )M) cadmium chloride concentrations, mimicking unpolluted, moderately and highly...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-008-9066-x
更新日期：2008-12-01 00:00:00
abstract：:The effects of aplysiatoxin and debromoaplysiatoxin on the clonal growth rate, cross-linked envelope formation and plasminogen activator secretion of normal human bronchial epithelial cells were studied. Neither compound was mitogenic over a wide range of concentrations (10(-13) to 10(-7)M). Both aplysiatoxin and debr...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00125571
更新日期：1984-10-01 00:00:00
abstract：:In a comprehensive assessment of genotoxicity, sodium fluoride was evaluated in a battery of cellular tests providing different genetic end points and biotransformation capabilities. The tests included the following: rat hepatocyte primary culture/DNA repair assay, Salmonella typhimurium histidine locus reversion assa...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00119244
更新日期：1988-06-01 00:00:00
