Abstract:
:Ten years have passed since the first publication announcing the generation of induced pluripotent stem cells (iPSCs). Issues related to ethics, immune rejection, and cell availability seemed to be solved following this breakthrough. The development of iPSC technology allows advances in in vitro cell differentiation for cell therapy purpose and other clinical applications. This review provides a perspective on the iPSC potential for cell therapies, particularly for hematological applications. We discuss the advances in in vitro hematopoietic differentiation, the possibilities to employ iPSC in hematology studies, and their potential clinical application in hematologic diseases. The generation of red blood cells and functional T cells and the genome editing technology applied to mutation correction are also covered. We highlight some of the requirements and obstacles to be overcome before translating these cells from research to the clinic, for instance, iPSC variability, genotoxicity, the differentiation process, and engraftment. Also, we evaluate the patent landscape and compile the clinical trials in the field of pluripotent stem cells. Currently, we know much more about iPSC than in 2006, but there are still challenges that must be solved. A greater understanding of molecular mechanisms underlying the generation of hematopoietic stem cells is necessary to produce suitable and transplantable hematopoietic stem progenitor cells from iPSC.
journal_name
Cell Biol Toxicoljournal_title
Cell biology and toxicologyauthors
Paes BCMF,Moço PD,Pereira CG,Porto GS,de Sousa Russo EM,Reis LCJ,Covas DT,Picanço-Castro Vdoi
10.1007/s10565-016-9377-2subject
Has Abstractpub_date
2017-06-01 00:00:00pages
233-250issue
3eissn
0742-2091issn
1573-6822pii
10.1007/s10565-016-9377-2journal_volume
33pub_type
杂志文章,评审abstract::Skin intoxication by bis(beta-chloroethyl)sulfide (BCES; sulfur mustard) induces cutaneous lesions similar to thermal burns, characterized by slowness of skin healing. We have developed an in vitro model of skin equivalent to investigate mechanisms involved in this delay. Direct intoxication of dermal equivalent produ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1023/a:1007491703271
更新日期:1998-02-01 00:00:00
abstract::Sister chromatid exchange rate was studied in 12 early diagnosed cases of nasopharyngeal carcinoma and in their paired controls. Exchange frequencies were also analyzed in 100 healthy subjects distributed in four regions of Hunan Province and correlated to nationality, age and sex. The incidence of sister chromatid ex...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00117868
更新日期:1987-09-01 00:00:00
abstract::Microfilaments and microtubules are components of the cytoskeleton which could be implicated in neoplastic transformation. We studied the effect of two hepatic tumor promoters, phenobarbital (PB) and biliverdin (BV), on microfilaments and microtubules of non-transformed (Cl3) and transformed (FV) hepatic epithelial ce...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00135024
更新日期:1990-01-01 00:00:00
abstract::Lung cancer is one of the most common malignancies worldwide. Actinidia chinensis Planch root extract (acRoots) was found to have the capacity of the anti-tumor, although the molecular mechanisms remain unclear. The present study aims to investigate the molecular mechanisms by which lung cancer cells sense to inhibito...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-018-9422-4
更新日期:2018-06-01 00:00:00
abstract::To determine the toxicological effects of complex mixtures of pesticides, we obtained data on 100 pesticide residues in common foods of central Italy. Fifteen pesticides were more regularly detected at higher levels (dithiocarbamates, benomyl/carbendazim, thiabendazole, diphenylamine, chlorthalonil, procymidone, fenar...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00754461
更新日期:1993-10-01 00:00:00
abstract::One of the major objectives at the end of this century is the development of "alternative" tests for the evaluation of the pharmacological and/or toxicological activity of newly developed molecules. Contact allergy is no exception to the rule and many research programs have been started to develop in vitro techniques ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章,评审
doi:10.1023/a:1007502723275
更新日期:1999-02-01 00:00:00
abstract::The mutagenic activity of the pyrolysis products 2-amino-3-methyl-imidazo[4,5-f]-quinoline 2-amino-3,4-dimethylimidazo[4,5-f]-quinoline in Salmonella typhimurium TA98 using rat intestinal and renal subcellular fractions as activation systems was approximately 1 and 5 revertants per nmol, respectively. This was 1,000 t...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00117825
更新日期:1987-03-01 00:00:00
abstract::Deoxynivalenol (DON), nivalenol (NIV), T-2 toxin (T2), fumonisin B1 (FB1), zearalenone (ZEA), and moniliformin (MON) mycotoxins are common food and feed contaminants produced by Fusarium spp. However, while they are usually found to co-occur in a large range of commodities, only few data are available on mycotoxin co-...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-017-9408-7
更新日期:2018-06-01 00:00:00
abstract::The effects of the oxidizing agent t-butylhydroperoxide (t-BHP) were investigated on three human cell lines of different origin and growth features (A431 epithelial cells, ADF astrocytoma cells and U937 leukemic cells) using electron microscopy and electron paramagnetic resonance spectroscopy. The results indicate tha...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00756761
更新日期:1994-08-01 00:00:00
abstract::[Pt(O,O'-acac)(γ-acac)(DMS)] (PtAcacDMS) is a new platinum compound showing low reactivity with nucleobases and specific reactivity with sulfur ligands intracellularly. It induces apoptosis in breast cancer cells, but appears to be less neurotoxic to the developing cerebellum than cisplatin (cisPt). The aim of this st...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-013-9252-3
更新日期:2013-10-01 00:00:00
abstract::Adverse effect of nanoparticles may include impairment of phagocyte function. To identify the effect of nanoparticle size on uptake, cytotoxicity, chemotaxis, cytokine secretion, phagocytosis, oxidative burst, nitric oxide production and myeloperoxidase release, leukocytes isolated from human peripheral blood, monocyt...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-013-9265-y
更新日期:2014-02-01 00:00:00
abstract::The purpose of the study was to determine whether along and in combination melatonin (MLT) and pentoxifylline (PTX) exerted beneficial effects on histopathological changes and changes in oxidant and antioxidant systems in liver caused by CCl4-induced liver toxicity in mice. Mice were randomly divided into six groups: ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-006-0019-y
更新日期:2006-11-01 00:00:00
abstract::During the last few years the increased presence of proinflammatory cytokines and oxidation products in Alzheimer's disease (AD) has been largely described. Oxysterols, formed by hydroxylation of cholesterol, occur naturally in the brain and are increased in the serum of AD patients. Of these oxysterols, 25-hydroxycho...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1023/a:1011996515328
更新日期:2001-01-01 00:00:00
abstract::Health risks associated with the inhalation of potentially toxic materials have been a topic of great public concern. In vitro cellular analyses can provide mechanistic information on the molecular-level responses of lung-derived cell lines to a variety of these hazards. This understanding may be used to develop metho...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-006-0150-9
更新日期:2006-03-01 00:00:00
abstract::Reciprocal interactions between tumor cells and endothelial cells constitute the most important stage of tumor metastasis. There is growing evidence suggesting that beta-estradiol and vitamin D modulate the progression of steroid-sensitive breast cancers. In keeping with those results, the purpose of the study reporte...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-005-0002-z
更新日期:2005-09-01 00:00:00
abstract::Recent studies have demonstrated that the insecticide DDT is a tumor promoting agent. Similar to many other tumor promoting agents, DDT has been shown to inhibit gap junctional intercellular communication (GJIC) between cells in culture, and it has been suggested that DDT-induced loss of communication between adjacent...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00249596
更新日期:1990-04-01 00:00:00
abstract::Hepatocytes have the ability to go through specialized cell cycles, which, during normal developmental liver growth, result in the formation of binuclear and polyploid cells. In the adult rat liver, the majority of the hepatocytes (about 70%) are tetraploid, 15-20% are octoploid, and only 10-15% are diploid (about 50%...
journal_title:Cell biology and toxicology
pub_type: 杂志文章,评审
doi:10.1023/a:1007487425047
更新日期:1997-07-01 00:00:00
abstract::In pharmaceutical research, in vitro toxicity tests, for assessing the potential toxicity of new chemical entities are necessary in the early stages of the developmental process, when no information is available about the metabolism or even the target organ toxicity of the compounds to be tested. In vitro specific org...
journal_title:Cell biology and toxicology
pub_type: 杂志文章,评审
doi:10.1023/a:1010958121396
更新日期:2001-01-01 00:00:00
abstract::The mesothelial cells (MCs) play an important role in the morpho-functional alterations of the peritoneal membrane (PM) undergoing peritoneal dialysis (PD). MCs, through the epithelial-mesenchymal transition process (EMT), progressively acquire a myofibroblast-like phenotype, promoting peritoneal fibrosis (PF) and fai...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-018-9438-9
更新日期:2019-04-01 00:00:00
abstract::Previous studies showed that the pesticide lindane (gamma-hexachlorocyclohexane) inhibits gap junction intercellular communication in rat myometrial cells. The present study tested the hypothesis that lindane and the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) inhibit gap junction communication in rat myo...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1023/b:cbto.0000029465.74815.62
更新日期:2004-05-01 00:00:00
abstract::Metolachlor, a commonly used herbicide in the Midwestern USA, functions by inhibiting chlorophyll and protein synthesis in target plants. Herbicide exposure has led to detrimental effects in several organisms, affecting their growth and behavior; however, its mechanism of action in nontarget organisms is not yet clear...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-013-9256-z
更新日期:2013-08-01 00:00:00
abstract::This study was implemented to test the Episkin model of reconstructed epidermis in the evaluation of the efficacy of cosmetic or dermopharmaceutical products on cutaneous energy metabolism. The energy metabolism is evaluated by measuring the concentration of intracellular ATP by a method using an ultrasensitive biolum...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00755786
更新日期:1994-12-01 00:00:00
abstract::In previous studies cadmium chloride (CdCl2) nonlethally inhibited Y-1 adrenal mouse adrenal tumour cell 20-dihydroxyprogesterone (20DHP) secretion, affecting unstimulated and stimulated steroidogenic pathway sites differently. We studied CdCl2 effects on unstimulated steroidogenesis using Y-1 cells incubated 0.5 h in...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00757184
更新日期:1994-02-01 00:00:00
abstract::Presently, a large effort is being made worldwide to increase the sustainability of industrial development, while preserving not only the quality of the environment but also that of animal and human life. In this work, sea urchin early developmental stages were used as a model to test the effects of the organophosphat...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-008-9061-2
更新日期:2008-12-01 00:00:00
abstract::Knowledge of the acute toxic effects of chemicals is important for the protection of exposed humans. Since sufficient information in humans is often lacking, experiments on laboratory animals must be performed. The LD50 test, which requires large numbers of animals, has become the preferred procedure. It is now widely...
journal_title:Cell biology and toxicology
pub_type: 杂志文章,评审
doi:10.1007/BF00121848
更新日期:1986-09-01 00:00:00
abstract::Various in vitro preparations have been utilized to study the cellular activity of vasoactive agents on renal cortical microvessels and on mesangial cells. The receptors and the transduction pathways of bradykinin and atrial natriuretic factor were characterized on cultured cortical vascular smooth muscle cells from t...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00438157
更新日期:1996-12-01 00:00:00
abstract::Transforming growth factor-beta1 (TGF-beta1) can activate mitogen-activated protein kinases (MAPKs) in many types of cells. The mechanism of this activation is not well elucidated. Here, we explore the role of TGF-beta/Smads signaling compounds in TGF-beta1-mediated activation of extracellular signal-regulated kinase ...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-006-0097-x
更新日期:2007-03-01 00:00:00
abstract::The risk presented by mycotoxins is a toxicological problem. As the data given by physico-chemical analysis may be difficult to translate in terms of toxicity, however, especially when considering multiple contamination, we have developed a system for toxicological analysis of mycotoxins using cell cultures of differe...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/BF00122655
更新日期:1989-06-01 00:00:00
abstract::The mutation rates of tumor suppressor protein p53 gene (TP53) are high in lung adenocarcinoma and promote the development of acquired drug resistance. The present study evaluated the p53-dependent role in lung cancer cell sensitivity to PI3K-specific inhibitors, PI3K-associated inhibitors, PI3K-non-related inhibitors...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1007/s10565-020-09523-7
更新日期:2020-10-01 00:00:00
abstract::The kinetic parameters of inhibition of camel retinal acetylcholinesterase (AChE) activity by cycloheximide (CH) were investigated. For the control system, the Michaelis-Menten constant (K(m)) for the hydrolysis of acetylthiocholine iodide was found to be 0.076 mmol/L and the Vmax was 0.547 mumol/min per mg protein. I...
journal_title:Cell biology and toxicology
pub_type: 杂志文章
doi:10.1023/a:1007421124793
更新日期:1998-06-01 00:00:00