Abstract：
:A method for the primary culture of rat liver cells on collagen-coated dextran microcarriers is described. Ethoxycoumarin deethylase (EOD) activity 24 hr after inoculation was comparable for liver cells cultured on microcarriers and on collagen-coated dishes. Cells were cultured on microcarriers for up to 48 hr and retained 25% of the initial EOD-activity that was seen in freshly isolated liver cells. Microcarrier-attached hepatocytes were cocultured with BALB/c 3T3 cells to study the metabolism-mediated cytotoxicity of cyclophosphamide (CPA). In the absence of hepatocytes, growth of 3T3 cells was not affected by CPA at concentrations up to 3600 microM. In coculture with hepatocytes, cytotoxicity of CPA was expressed in a time- and concentration-dependent manner. At high concentrations, CPA slightly depressed the EOD-activity of hepatocytes. Our results indicate that cocultivation of microcarrier-attached rat liver cells with target cells represents a valuable approach to the study of the metabolism-mediated toxicity of xenobiotics in vitro.
journal_name
Cell Biol Toxicoljournal_title
Cell biology and toxicologyauthors
Voss JU,Seibert Hdoi
10.1007/BF00124073subject
Has Abstractpub_date
1991-10-01 00:00:00pages
387-99issue
4eissn
0742-2091issn
1573-6822journal_volume
7pub_type
杂志文章abstract：:In vitro directional migration of 10T1/2 fibroblasts is partially inhibited by TPA but not by its non-promoting analogues. Other tumor promoters, e.g., phenobarbital, saccharin, and benzoylperoxide had no measurable effect when added in concentrations known to affect in vitro two-step transformation or intercellular c...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00120161
更新日期：1985-06-01 00:00:00
abstract：:Echinoderms are valuable test species in marine ecotoxicology and offer a wide range of biological processes appropriate for this approach. Regenerating echinoderms can be regarded as amenable experimental models for testing the effects of exposure to contaminants, particularly endocrine disrupter compounds (EDCs). As...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-008-9057-y
更新日期：2008-12-01 00:00:00
abstract：:The mutation rates of tumor suppressor protein p53 gene (TP53) are high in lung adenocarcinoma and promote the development of acquired drug resistance. The present study evaluated the p53-dependent role in lung cancer cell sensitivity to PI3K-specific inhibitors, PI3K-associated inhibitors, PI3K-non-related inhibitors...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-020-09523-7
更新日期：2020-10-01 00:00:00
abstract：:Interactions between microtubules and filamentous actin (F-actin) are essential to many cellular processes, but their mechanisms are poorly understood. We investigated possible roles of the myosin family of proteins in the interactions between filamentous actin (F-actin) and microtubules of budding yeast Saccharomyces...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1023/a:1013748500662
更新日期：2001-01-01 00:00:00
abstract：:The polyether toxin, okadaic acid, causes diarrhetic shellfish poisoning in humans. Despite extensive research into its cellular targets using rodent models, we know little about its putative effect(s) on innate immunity. We inoculated larvae of the greater wax moth, Galleria mellonella, with physiologically relevant ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-018-09448-2
更新日期：2019-06-01 00:00:00
abstract：:Although estrogens have been detected in some echinoderm species, their role is not clearly understood; so we examined the effects of estrogens administered to sea urchin embryos and larvae. A typical malformation was exogastrulation, induced by the exposure to ethynylestradiol (EER) in a defined period of 12 h from 1...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-008-9073-y
更新日期：2008-12-01 00:00:00
abstract：:DNA formulated into aggregates with polycationic reagents are referred to by a variety of terms including non-viral vectors, synthetic vectors, lipoplexes, polyplexes and more recently nanoparticles. The capacity for delivery of multiple genes, genomic-sized constructs and siRNA delivery, with a diversity of possible ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章,评审
doi：10.1007/s10565-009-9141-y
更新日期：2010-02-01 00:00:00
abstract：:Because of a possible role of astrocytes in trialkyltin-induced neurotoxicity in vivo various studies have been performed using cultures of astrocytes or glioma cells in vitro. With respect to cytotoxic potencies of trialkyltins these studies gave rather divergent results. Therefore the aim of the present study was to...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-004-3859-3
更新日期：2004-09-01 00:00:00
abstract：:Transforming growth factor-beta1 (TGF-beta1) can activate mitogen-activated protein kinases (MAPKs) in many types of cells. The mechanism of this activation is not well elucidated. Here, we explore the role of TGF-beta/Smads signaling compounds in TGF-beta1-mediated activation of extracellular signal-regulated kinase ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-006-0097-x
更新日期：2007-03-01 00:00:00
abstract：:Mechanisms of cell death induced by toxins probably involve one or more processes such as inhibition of protein synthesis and impairment of plasma membrane integrity leading to an increase in membrane permeability. Since one of the possible actions of mastoparan, a cationic tetradecapeptide from wasp venom, is to pert...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00135029
更新日期：1990-01-01 00:00:00
abstract：:Okadaic acid (OA), produced by dinoflagellates during harmful algal blooms (HAB), belongs to the Diarrheic Shellfish Poisoning toxins that cause gastrointestinal symptoms in humans after consumption. In the present work, Ruditapes decussatus haemocytes were selected to evaluate the effect of OA on cell viability, enzy...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-013-9246-1
更新日期：2013-06-01 00:00:00
abstract：:Magnesium, an essential metal that is important in the normal functioning of DNA, has been shown to interact with some of the toxic heavy metals in respect to biochemical and molecular mechanisms and in altering the tumorigenic process. This study examined the influence of magnesium in combination with nickel and cadm...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00756493
更新日期：1994-04-01 00:00:00
abstract：:An in vitro human nasal model was developed as a tool to study the local tolerability of nasal powder forms using excised nasal mucosa in a diffusion chamber. The suitability of this model was tested using Sandostatin (SMS) an octapeptide analog of somatostatin, as a reference drug enhanced by Avicel (microcrystalline...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00757627
更新日期：1995-10-01 00:00:00
abstract：:Owing to the excellent antibacterial and antiviral activity, silver nanoparticles have a widespread use in the food and pharmaceutical industries. With the increase in the production and use of the related products, the potential hazard of silver nanoparticles has aroused public attention. The main purpose of this stu...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-020-09526-4
更新日期：2020-05-04 00:00:00
abstract：:The effect of noncytotoxic doses of argemone oil (AO) and butter yellow (BY), the common adulterants in edible oil, on free radical generation and signaling pathway for cell proliferation in primary cells of gall bladder (GB) was undertaken. AO and BY showed no cytotoxicity at 0.1 μl/ml and 0.1 μg/ml concentration, re...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-011-9207-5
更新日期：2012-06-01 00:00:00
abstract：BACKGROUND AND AIMS:Endoplasmic reticulum (ER) stress is a growing concern for drug-induced toxicity which causes several side effects. Ritonavir, a potent HIV protease inhibitor, induces both ER and mitochondrial stress; however, the missing link between ER stress and mitochondrial damage has been unknown. In the pres...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-018-09451-7
更新日期：2019-06-01 00:00:00
abstract：:Aldehyde dehydrogenase was measured in primary cultures of hepatocytes obtained with a two-step collagenase perfusion either from human hepatic tissue or from livers of Fisher rats. Basal enzyme activity declines gradually as a function of time in culture, but remains at all times higher when measured with propionalde...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00122694
更新日期：1986-06-01 00:00:00
abstract：:The cytogenetic and hepatotoxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on mouse liver cells were investigated. Male C57BL/6J strain mice, which have TCDD receptors, were given single intraperitoneal injections of 25, 37.5, 75 and 150 micrograms of TCDD/kg body weight or corn oil carrier alone. Two-third...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00141285
更新日期：1988-03-01 00:00:00
abstract：:A tissue system in which fibroblasts and myocytes from chick embryonic hearts were separately maintained was used to study the toxicity of ethanol. To reproduce the teratogenic effects of acute, high concentrations of ethanol typical of "binge" drinking, an open tissue culture system was employed. With open cultures, ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00119291
更新日期：1992-01-01 00:00:00
abstract：:The effects of the oxidizing agent t-butylhydroperoxide (t-BHP) were investigated on three human cell lines of different origin and growth features (A431 epithelial cells, ADF astrocytoma cells and U937 leukemic cells) using electron microscopy and electron paramagnetic resonance spectroscopy. The results indicate tha...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00756761
更新日期：1994-08-01 00:00:00
abstract：:Generating large amounts of recombinant protein in transgenic animals is often challenging and has a number of drawbacks compared to cell culture systems. The baculovirus expression vector system (BEVS) uses virus-infected insect cells to produce recombinant proteins to high levels, and these are usually processed in ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-009-9133-y
更新日期：2010-02-01 00:00:00
abstract：:The mutagenic activity of the pyrolysis products 2-amino-3-methyl-imidazo[4,5-f]-quinoline 2-amino-3,4-dimethylimidazo[4,5-f]-quinoline in Salmonella typhimurium TA98 using rat intestinal and renal subcellular fractions as activation systems was approximately 1 and 5 revertants per nmol, respectively. This was 1,000 t...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00117825
更新日期：1987-03-01 00:00:00
abstract：:Bisnaphthalimido compounds bis-intercalate to DNA via the major groove and are potentially potent cancer therapeutics. Previously, we incorporated natural polyamines as linkers connecting the two naphthalimido ring moieties to create a series of soluble bisnaphthalimidopropyl polyamines (BNIPPs). Here, extending earli...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-011-9199-1
更新日期：2011-12-01 00:00:00
abstract：:In the US alone, around 60,000 lives/year are lost to colon cancer. In order to study the mechanisms of colon carcinogenesis, in vitro model systems are required in addition to in vivo models. Towards this end, we have used the HT-29 colon cancer cells, cultured in Dulbecco's Modified Eagle Medium (DMEM), which were e...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-020-09579-5
更新日期：2021-01-07 00:00:00
abstract：:Various in vitro preparations have been utilized to study the cellular activity of vasoactive agents on renal cortical microvessels and on mesangial cells. The receptors and the transduction pathways of bradykinin and atrial natriuretic factor were characterized on cultured cortical vascular smooth muscle cells from t...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00438157
更新日期：1996-12-01 00:00:00
abstract：:Ten years have passed since the first publication announcing the generation of induced pluripotent stem cells (iPSCs). Issues related to ethics, immune rejection, and cell availability seemed to be solved following this breakthrough. The development of iPSC technology allows advances in in vitro cell differentiation f...
journal_title：Cell biology and toxicology
pub_type： 杂志文章,评审
doi：10.1007/s10565-016-9377-2
更新日期：2017-06-01 00:00:00
abstract：:The histopathological response and cell culture characteristics of liver cells from the R16 (grc-) strain of rats, which carries an MHC-linked deletion, were examined one week after a single intraperitoneal injection of 200 mg/kg body weight diethylnitrosamine (DEN) and were compared with the response of liver cells f...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/BF00249594
更新日期：1990-04-01 00:00:00
abstract：:Microcystin-leucine-arginine (MC-LR) was produced by toxic cyanobacteria, which has been shown to have potent hepatotoxicity. Our previous study has proved that MC-LR were able to promote intrahepatic biliary epithelial cell excessive proliferation. However, the underlying mechanism is not yet entirely clarified. Here...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-020-09575-9
更新日期：2021-01-21 00:00:00
abstract：:Although methylguanine-DNA-methyltransferase (MGMT) plays an important role in resistance to temozolomide (TMZ) in glioma, 40% of gliomas with MGMT inactivation are still resistant to TMZ. The underlying mechanism is not clear. Here, we report that forkhead box M1 (FoxM1) transcriptionally activates the expression of ...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-017-9381-1
更新日期：2017-12-01 00:00:00
abstract：:Drug-induced liver injury (DILI) is a leading cause of discontinuation of new drug approval or withdrawal of marketed medicine based on safety due to organ vulnerability. The aim of this research is to investigate the potential abilities of four different in vitro cell models (L-02, HepG2, HepaRG, and hiHeps cell line...
journal_title：Cell biology and toxicology
pub_type： 杂志文章
doi：10.1007/s10565-016-9316-2
更新日期：2016-02-01 00:00:00
