Abstract:
:Myelin basic protein (MBP)-specific SJL/J T cells were cultured in normal growth medium or growth medium supplemented with 10% culture supernatant from WEHI-3 cells, a source of interleukin-3 (IL-3), or with recombinant IL-3. T cell lines cultured with IL-3 supplementation were more encephalitogenic compared to parallel lines cultured without this supplement. There was little difference in antigen-specific proliferative response or expression of cell surface markers CD3, CD4, CD8, IL-2R, or alpha/beta TCR in the parallel lines. Supernatant fluids from antigen-stimulated T cells from each cycle were tested for the presence of IL-2, IL-3, IL-4, granulocyte macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor-alpha (TNF alpha/beta) and transforming growth factor-beta (TGF beta). No significant difference in IL-2, IL-4, GM-CSF, TNF alpha/beta, or TGF beta levels were seen when supplemented and unsupplemented cultures were compared. Supernatant culture fluids contained an activity that was highly stimulatory for the IL-3-dependent mouse mast cell line, MC/9. This activity was attributable to a combination of at least three factors that varied in relative concentrations throughout the course of the experiments. Based on neutralization by monoclonal antibodies, MC/9 stimulating activity in early passage lines was attributable entirely to IL-3 and GM-CSF. The fraction of the MC/9 stimulatory activity that could be neutralized by monoclonal antibody to IL-3 decreased with increasing stimulation cycle while the fraction neutralized by anti-GM-CSF antibodies remained relatively constant. At the time that the lines lost encephalitogenicity, the activity neutralizable by anti-IL-3 had dropped to low levels in the culture supernatants; however, MC/9 stimulatory activity remained present in the supernatants. This was due to GM-CSF and a third unidentified factor.
journal_name
J Neuroimmunoljournal_title
Journal of neuroimmunologyauthors
Zhao ML,Xia JQ,Fritz RBdoi
10.1016/0165-5728(93)90076-bsubject
Has Abstractpub_date
1993-03-01 00:00:00pages
69-78issue
1-2eissn
0165-5728issn
1872-8421pii
0165-5728(93)90076-Bjournal_volume
43pub_type
杂志文章abstract::The timing and intensity of the antibody responses to the marker determinants of synthetic peptide S81 and S82 sequences of bovine myelin basic protein (MBP) (residues 68-83 and 65-83, respectively) were studied in 20 Lewis rats and 6 rabbits. All rats immunized with either peptide in CFA responded with antibody devel...
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pub_type: 杂志文章
doi:10.1016/0165-5728(81)90045-x
更新日期:1981-06-01 00:00:00
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abstract::Depression and chronic fatigue are frequently present in multiple sclerosis (MS); however, the prevalence rates have not been investigated in neuromyelitis optica (NMO). Thirty-nine consecutive NMO and 75 MS patients were compared using self-rating questionnaires for depressive states, daily activity, and fatigue, as ...
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更新日期:2015-06-15 00:00:00
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journal_title:Journal of neuroimmunology
pub_type: 杂志文章
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更新日期:2015-05-15 00:00:00
abstract:OBJECTIVE:Our aim was to investigate the correlation between onset age, clinical features and HLA-DQA1/DQB1 genetic variability in myasthenia gravis (MG) patients in Southern Han Chinese. METHODS:205 MG patients and 100 controls were genotyped for HLA-DQA1 and -DQB1 using sequence-based typing (SBT) and analyzed for h...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2012.03.018
更新日期:2012-06-15 00:00:00
abstract::The Lyme disease agent Borrelia garinii has been suggested to be neurotrop, preferentially affecting the nervous system. We compared the secretion of interferon-gamma in response to outer surface proteins from Borrelia garinii and Borrelia afzelii in 10 patients with neuroborreliosis. In cerebrospinal fluid, stimulati...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(98)00136-2
更新日期:1998-08-14 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.jneuroim.2013.01.011
更新日期:2013-04-15 00:00:00
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journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(97)00120-3
更新日期:1997-11-01 00:00:00
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journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(84)80002-8
更新日期:1984-11-01 00:00:00
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journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(94)90240-2
更新日期:1994-10-01 00:00:00
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journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2004.12.015
更新日期:2005-04-01 00:00:00
abstract::Tumor necrosis factor alpha (TNFalpha) activity was measured in serum and cerebrospinal fluid (CSF) of Lewis rats after experimental allergic encephalomyelitis (EAE) induction and during the clinical course of acute disease. TNFalpha bioactivity expression preceded the clinical symptoms and paralleled the severity of ...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(95)00151-4
更新日期:1996-01-01 00:00:00
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journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(90)90039-p
更新日期:1990-06-01 00:00:00
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journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(86)90114-1
更新日期:1986-04-01 00:00:00
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journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(96)00012-4
更新日期:1996-05-01 00:00:00
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journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(99)00100-9
更新日期:1999-08-03 00:00:00
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journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2005.06.008
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pub_type: 杂志文章
doi:10.1016/j.jneuroim.2013.07.016
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journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2005.07.008
更新日期:2005-11-01 00:00:00
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journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(94)00159-l
更新日期:1995-03-01 00:00:00
abstract::Rituximab has been effectively used for treating neuromyelitis optica spectrum disorder (NMOSD) for several years. However those regimens exert a heavy burden on Chinese patients. The aim of our study was to investigate an effectiveness, economic alternatives of RTX. The enrolled patients received different immunosupp...
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pub_type: 临床试验,杂志文章
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更新日期:2018-04-15 00:00:00
abstract::Increases in spontaneous sister chromatid exchange (SCE) and gamma radiation-induced chromosome aberrations have been reported in peripheral blood lymphocytes (PBL) from multiple sclerosis (MS) patients, suggesting the presence of an abnormality in repair in this disease. We tested this hypothesis by measuring the abi...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(89)90155-0
更新日期:1989-01-01 00:00:00
abstract::Although the pathogenesis of multiple sclerosis (MS) is not fully understood, substantial evidence points to the involvement of genetic factors. We report on a genome-wide screen for disease association in the Hungarian population using 5532 microsatellite markers. These markers were typed in DNA pools that consisted ...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2003.08.017
更新日期:2003-10-01 00:00:00
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journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2007.06.016
更新日期:2007-09-01 00:00:00
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journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(00)00365-9
更新日期:2000-11-01 00:00:00
abstract::A significant skewing of the peripheral T cell repertoire has been shown in relapsing-remitting multiple sclerosis (MS). Most of the studies already performed in this field are cross-sectional and therefore, little is known of the T cell repertoire evolution over time in MS and the correlation of T cell repertoire var...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2006.05.006
更新日期:2006-08-01 00:00:00
abstract::We recently showed that MMP-9 activity was detectable in the cerebrospinal fluid (CSF) of about half of neurologically symptomatic HIV-infected patients. Using an experimental animal model, we detected MMP-9 activity in CSF samples from rats that had been injected intracisternally with recombinant HIV-1 Nef protein, b...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(99)00170-8
更新日期:2000-01-24 00:00:00
abstract::The inflammatory reaction in autoimmune polymyositis and rejection of transplanted myoblasts is characterized by mononuclear cell infiltration. In other settings monocytes are locally recruited by an IL-6-induced IL-8-to-MCP-1 switch. IL-6, upon binding to soluble gp80 (sIL-6R), can interact with membrane-bound ubiqui...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2008.02.005
更新日期:2008-05-30 00:00:00