Abstract:
:The mRNA level of the 17-kDa protein neurogranin (NG), a postsynaptic substrate of the protein kinase C, has previously been found to be decreased in rat forebrain after 24-h sleep deprivation (SD). To investigate the functional significance of this finding in various forebrain regions, the effect of 24-h SD on the mRNA level and the protein level of NG was determined in the cerebral cortex, hippocampus, and the total of the remaining subcortical forebrain plus midbrain areas (SFMA) of rats. In these areas, high levels of both NG mRNA and NG protein were detected by in situ hybridization and immunohistochemistry, respectively. NG protein was recognized in brain tissue by newly developed polyclonal antibodies. As determined by RNase protection assays, the level of NG mRNA was decreased in SFMA by 34 +/- 7% (P < 0.05) after 24-h SD, and was not significantly affected in the cerebral cortex and hippocampus. In contrast, on Western blots, the protein concentration of NG was reduced in the cerebral cortex by 37 +/- 7% (P < 0.05) whereas no significant changes were present in other brain areas tested. The results indicate that the mRNA and protein levels of NG are differentially modulated in rat brain by the prolongation of the waking period.
journal_name
Brain Resjournal_title
Brain researchauthors
Neuner-Jehle M,Rhyner TA,Borbély AAdoi
10.1016/0006-8993(95)00416-nsubject
Has Abstractpub_date
1995-07-10 00:00:00pages
143-53issue
1-2eissn
0006-8993issn
1872-6240pii
0006-8993(95)00416-Njournal_volume
685pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::We report the localization of angiotensin-converting enzyme (kininase II, EC 3.4.15.1) in discrete nuclei and areas of the rat brain by a quantitative autoradiographic technique using image processing coupled to computerized microdensitometry, after incubation of brain sections with the specific converting enzyme inhi...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(86)90746-8
更新日期:1986-06-11 00:00:00
abstract::Epidemiological studies have shown that steroidal as well as non-steroidal anti-inflammatory drugs lower the risk of developing Alzheimer's Disease (AD). A suppressive effect of these anti-inflammatory drugs on local inflammatory events in AD brains has been suggested, however the mechanisms responsible are still unkn...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)01204-3
更新日期:1997-11-28 00:00:00
abstract::By using enkephalin immunohistochemistry combined with retrograde fluorescent labelling, a great majority of neurons in the rat cerebellum sending their axons to the spinal cord were shown to contain enkephalin immunoreactivity. These neurons were numerous and clustered in the fastigial nucleus but far less abundant i...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(95)00557-7
更新日期:1995-09-04 00:00:00
abstract::Intracerebroventricular (i.c.v.) infusion of sodium in rats increases cerebrospinal fluid (CSF) [Na], mimicking the effects of a high salt diet in salt-sensitive strains and causing sympathetic hyperactivity and a pressor response that are mediated via both an endogenous brain ouabainlike substance (OLS) and the brain...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2004.06.056
更新日期:2004-09-24 00:00:00
abstract:BACKGROUND:Previous experimental work using in situ zymography has shown very early increased plasminogen activation in ischemic regions after 3 h of ischemia with and without reperfusion. The objective of the present study was to evaluate the time course and extent of plasminogen activation in long-term permanent foca...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(00)02769-4
更新日期:2000-11-03 00:00:00
abstract::Primary rat neuronal cultures can be preconditioned against ischemic damage by several mechanisms. In the present study we established a new model system in order to characterize the "time window of protection' obtained by preconditioning of neurons with adenosine. Ischemia was simulated by exposure of the cultures to...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(96)00939-0
更新日期:1996-11-25 00:00:00
abstract::One of the fundamental tasks of neurobiology is to understand how the precision and specificity of the adult nervous system is achieved during development. This paper reviews the progress that has been made toward this end in studies of the developing mammalian cerebral cortex. Particular attention is focused on the p...
journal_title:Brain research
pub_type: 杂志文章,评审
doi:10.1016/0165-0173(88)90002-1
更新日期:1988-01-01 00:00:00
abstract::Distribution of adult T cell leukemia derived factor (ADF/TRX), a human thioredoxin homologue, was studied by immunohistochemistry in gerbil brain during reperfusion after transient cerebral ischemia. In control brains, immunoreactivity was observed widely in the central nervous system, including the ependyma, tanycyt...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)90130-f
更新日期:1993-10-15 00:00:00
abstract::Brain-derived angiotensin II (ANG II) and prostaglandins have important roles in the regulation of body fluid and blood pressure homeostasis. In the present studies we investigated the central interactions between these two neurochemical products in regulating the hypothalamo-neurohypophysial system during dehydration...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(00)03113-9
更新日期:2001-01-19 00:00:00
abstract::Recent developments in the understanding of the neurophysiological mechanisms underlying electroencephalographic (EEG) slow waves and sleep spindles imply an inverse relationship between these two EEG activities. The interrelationship between slow wave activity (0.75-4.5 Hz) and sleep-spindle activity (12-15 Hz) in el...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)90579-c
更新日期:1993-10-29 00:00:00
abstract::While exposure of cultured spinal neurons to mild hypothermia provides some protection from physical trauma (dendrotomy), profound cooling (< 17 degrees C) causes unrelated neuronal injury and death, which can be prevented by treatment with NMDA receptor antagonists. To investigate the mechanism of hypothermic neurona...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(95)00726-7
更新日期:1995-09-18 00:00:00
abstract::Potassium-evoked cholecystokinin (CCK) release from slices of caudate-putamen and cerebral cortex, but not hippocampus incubated in vitro was increased by 152-175% by preincubation for 40 min with 10 mM lithium. These results and previous studies suggest that although different physiological agents regulate CCK releas...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(87)91030-4
更新日期:1987-06-16 00:00:00
abstract::Treatment with thyrotropin-releasing hormone (TRH) significantly improved survival following induction of fatal systemic anaphylaxis in mice. The protective effect was mediated centrally since survival was increased by intracerebroventricular (i.c.v.) administration of TRH at doses which had no effect when given syste...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(84)91421-5
更新日期:1984-04-30 00:00:00
abstract::Increasing doses (0.1, 1.0, 10.0 mg/kg) of scopolamine were systemically (i.p.) administered to rats subjected to moderate fluid percussion brain injury. Scopolamine treatment (1.0 mg/kg, i.p.) 15 min prior to trauma significantly reduced mortality and the duration of transient behavioral suppression assessed by a var...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(88)91104-3
更新日期:1988-05-10 00:00:00
abstract::We sought to characterize regional heterogeneity of astrocytes using adrenergic receptor sites as cellular markers. Primary cultures made from 6 regions of neonatal rat brain consisted almost exclusively of astrocytes. Membranes from astrocytes cultured 1-3 weeks were prepared for radioligand binding assays of beta- a...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(90)91027-e
更新日期:1990-05-28 00:00:00
abstract::A feature of neurodegenerative disease is the accumulation of insoluble protein aggregates in the brain. In some conditions, including Amyotrophic Lateral Sclerosis and Frontotemporal lobar degeneration, the primary aggregating entities are RNA binding proteins. Through regulated prion-like assembly, RNA binding prote...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2014.05.052
更新日期:2014-10-10 00:00:00
abstract::In rats with unilateral 6-hydroxydopamine lesions of the substantia nigra, a specific D1 dopamine receptor agonist, SKF 38393A, at a dose that does not itself produce turning, significantly increased the contralateral rotation observed following a low dose of the specific D2 agonist LY 171555. Doses of SKF 38393A or t...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(86)91178-9
更新日期:1986-10-08 00:00:00
abstract::The pedunculopontine tegmental nucleus (PPT) has been implicated in the generation and/or maintenance of both active sleep (AS) and wakefulness (W). GABAergic neurons are present within this nucleus and recent studies have shown that these neurons are active during AS. In order to examine the role of mesopontine GABAe...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(02)02475-7
更新日期:2002-07-19 00:00:00
abstract::Occlusion of the abdominal aorta proximal to the renal arteries results in an increase in arterial blood pressure, inhibition of forepaw and hindpaw withdrawal to a noxious mechanical stimulus, and inhibition of the tail-flick reflex to noxious heat. Occlusion of the abdominal aorta distal to the renal arteries does n...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(90)90055-g
更新日期:1990-06-11 00:00:00
abstract::Here we describe the nerves innervating the perineal skin together with their sensory fields in the adult female rat. Electrophysiological recording showed that the lumbosacral and L6-S1 trunks, in part by way of the sacral plexus, transmit sensory information from the perineal skin via four nerves: the viscerocutaneo...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2004.07.046
更新日期:2004-10-22 00:00:00
abstract::Administration of methyl palmoxirate (MP), an inhibitor of fatty acid oxidation, stimulates eating behavior in rats. Fos immunohistochemistry was used to determine neural pathways that may play a role in the eating response to MP. The number of cells showing Fos-like immunoreactivity (Fos-li) was quantified by compute...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)01143-8
更新日期:1998-01-19 00:00:00
abstract::The current study explored the effects of prolonged antidepressant treatment on mRNA levels of two nuclear receptors in specific brain regions of an animal model of depression, the Wistar-Kyoto (WKY) rat. Both nuclear receptors have been implicated in the development or treatment of depression. The expression of nucle...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2010.09.041
更新日期:2010-11-29 00:00:00
abstract::For the purpose of obtaining chemical information about the physiological role of N-acetylaspartylglutamate (NAAG), the release of endogenous NAAG from and the uptake of [3H]NAAG by Guinea pig cerebellar slices were investigated in comparison with L-aspartate (Asp) and L-glutamate (Glu). Although endogenous NAAG was f...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(89)90544-1
更新日期:1989-03-13 00:00:00
abstract::Statistical analysis techniques that permit detection and quantification of EEG-single unit correlations were employed in a study of generalized penicillin epilepsy in the cat. Single unit activity was recorded in cerebral and cerebellar cortices and compared to the locally recorded EEG. It was found that during gener...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(78)90703-5
更新日期:1978-10-13 00:00:00
abstract::30-Day-old male Wistar rats were tested for acquisition and retention of operant conditioned behavior after bilateral subicular lesions made either electrolytically or chemically (ibotenic acid). The acquisition of operant learning was carried out in lesioned rats by assessing the number of sessions required to learn ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(96)01135-3
更新日期:1997-01-16 00:00:00
abstract::Recently we reported that chronic treatment with 17beta-estradiol (E2) or tamoxifen (TAM) regulates the ovariectomy-induced downregulation of the key molecules linked to hippocampal synaptic plasticity and signal transduction pathway. We now report modulation of the antiapoptotic (Bcl-2) and proapoptotic (Bax) protein...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2008.01.080
更新日期:2008-04-14 00:00:00
abstract::Neuropeptide-Y (NPY) cells in the intergeniculate leaflet (IGL) are known to modulate effects of arousal on the mammalian circadian system. However, the route through which this information reaches the IGL has not been established. Here, we provide evidence that the orexins (hypocretins) are uniquely positioned as a p...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2005.06.043
更新日期:2005-08-16 00:00:00
abstract::An extensive behavioral characterization was conducted with mice lacking the gene for neuropeptide Y (NPY) including response to 24 and 48 h fast and challenge with small molecule antagonists of NPY receptors implicated in mediating the feeding effects of NPY (i.e., Y1 and Y5). In addition, wildtype (WT) and NPY knock...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(00)02285-x
更新日期:2000-06-16 00:00:00
abstract::Visual pathways (optic disc, optic nerve and pretectal regions) were electrically stimulated and evoked potentials were explored throughout the inferior olive in the anesthetized rat. Responsive areas were identified as the caudal half of the dorsal cap, nucleus beta and the most caudal region of subnucleus c of the m...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(86)91060-7
更新日期:1986-03-12 00:00:00
abstract::Maternal melatonin readily crosses the placenta to provide the fetus with time-of-day and day length information. To determine if melatonin could have a broader role during development than is currently recognized, melatonin receptor expression was examined in somatic sites during Siberian hamster embryogenesis. Using...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)91424-y
更新日期:1991-12-24 00:00:00