Abstract:
:To assess the role interleukins and mitogens play in regulating immunoglobulin (Ig) gene expression via the Ig enhancer and promoter, transgenic mice carrying two different Ig gene regulatory regions were generated. One, EmukCAT, contains the Ig heavy chain enhancer (Emu) and the kappa light chain promoter driving the chloramphenicol acetyltransferase (CAT) gene. In the other, delta EmukCAT, CAT is under the control of the kappa promoter alone. Emu and kappa relative activity were assessed by CAT assay. In EmukCAT mice, low CAT expression was consistently found in spleen, bone marrow, mesenteric lymph node, and thymus but not in brain, lung, or kidney. In delta EmukCAT mice, CAT expression was detectable just above background in lymphoid tissues, suggesting a basic level of tissue specificity in the absence of the enhancer. Whole spleen cell cultures prepared from the mice were treated with lymphokines and mitogens. Lipopolysaccharide (LPS), concanavilin A (Con A), interleukin 6 (IL-6), and interferon-gamma (IFN-gamma) increased CAT expression to varying extents in cells derived from EmukCAT mice but not in spleen cells prepared from delta EmukCAT mice. Thus, the presence of Emu, in addition to the kappa promoter, is essential for the stimulation of CAT expression mediated by these factors. B cells from EmukCAT mice were separated by density into populations of small and large cells. In untreated small B cells, no CAT expression was detected and only addition of LPS resulted in an increase in CAT expression. In large B cells, CAT was expressed at a low level without addition of exogenous factors. Incubation with LPS, IL-6, Con A and IFN-gamma caused CAT expression to increase several-fold. This transgenic system provides a means to identify exogenous factors that activate Ig enhancers and promoters.
journal_name
Immunogeneticsjournal_title
Immunogeneticsauthors
Miller AE,Ennist DL,Ozato K,Westphal Hdoi
10.1007/BF00216623subject
Has Abstractpub_date
1992-01-01 00:00:00pages
24-32issue
1eissn
0093-7711issn
1432-1211journal_volume
35pub_type
杂志文章相关文献
IMMUNOGENETICS文献大全abstract::We report the sequence of a cDNA encoding a rabbit immunoglobulin gamma heavy chain of d12 and e14 allotypes with high homology to partial cDNA sequences from rabbits of d11 and e15 allotypes. The encoded rabbit protein shows homologies with human (68-70%) and mouse (60-63%) gamma chains. The nucleotide sequence homol...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00372471
更新日期:1983-01-01 00:00:00
abstract::Transcription of major histocompatibility complex class I genes is controlled by the class I regulatory complex in the 5' flanking region. To investigate the molecular basis of this region, we studied the polymorphism of the promoter of the HLA-B locus extending from the ATG transcription initiation signal to -284 bas...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00163991
更新日期:1995-01-01 00:00:00
abstract::Four cDNA probes for the human major histocompatibility complex (MHC) were used to investigate the sheep MHC, in conjunction with serological typing for ovine lymphocyte antigen (OLA). Lymphocytes from a family (two parents and five offspring) of Romanov sheep were subjected to genomic DNA digestion by the restriction...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00430918
更新日期:1985-01-01 00:00:00
abstract::The nucleotide sequences of 11 variable gene segments coding for rabbit T-cell receptor beta (Tcrb-V) chains were determined by directly sequencing fragments amplified by the cassette-ligation mediated polymerase chain reaction (CLM-PCR) and by modified anchor PCR without the cloning procedure. The nucleotide sequence...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00188786
更新日期:1994-01-01 00:00:00
abstract::The structure of the DRB1*03 gene has been interpreted as the product of a gene conversion event involving a DRB3 gene as donor and resulting in the introduction of two short segments of the DRB3 sequence into the DRB1 locus. The serological counterpart of this double insertion is the TR81 specificity. Consequently, t...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00717905
更新日期:1989-01-01 00:00:00
abstract::Six IgA allotypes are serologically identifiable in inbred mice. The sequences of the PCR-amplified C alpha 1, C alpha 2 and C alpha 3 exons from the genomic DNA of mice of four previously unsequenced allotypes already have been compared with those of BALB/c and of a wild mouse, Mus pahari, in the literature. Sporadic...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-002-0434-z
更新日期:2002-03-01 00:00:00
abstract::Binding of peptides to major histocompatibility complex (MHC) molecules is the single most selective step in the recognition of pathogens by the cellular immune system. The human MHC genomic region (called HLA) is extremely polymorphic comprising several thousand alleles, each encoding a distinct MHC molecule. The pot...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-008-0341-z
更新日期:2009-01-01 00:00:00
abstract::Parasites exert a selection pressure on their hosts and are accountable for driving diversity within gene families and immune gene polymorphisms in a host population. The overwhelming response of regulatory T cells during infectious challenges directs the host immune system to lose the ability to mount parasite specif...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-011-0524-x
更新日期:2011-07-01 00:00:00
abstract::The serological reactivities of HLA-A3, -B7, and -CW3 heavy chains associated with either mouse, bovine, or human beta-2-microglobulin (beta 2m) and expressed on the surface of transfected mouse fibroblasts were analyzed. All reactivities associated with one cluster (defined by monoclonal antibody W6/32) of antigenic ...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00430798
更新日期:1985-01-01 00:00:00
abstract::Alpha-2-macroglobulin (A2M) binds proteases, thereby acting as defense barriers against pathogens in the plasma and tissues of vertebrates and invertebrates. Quantitative real-time polymerase chain reaction (PCR) and the isobaric tags for relative and absolute quantitation method were used to determine the expression ...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-012-0639-8
更新日期:2012-11-01 00:00:00
abstract::Transgenic mice were produced in which human HLA-Cw4 is stably integrated, behaves as a single Mendelian trait, and, being under the transcriptional control of human CD2, is selectively and efficiently expressed in T lymphocytes. These mice were used as a model system to determine whether HLA-type C molecules can be e...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00179398
更新日期:1995-01-01 00:00:00
abstract::We reported previously that human gene MAGE-1 directs the expression of a tumor antigen recognized on a melanoma by autologous cytolytic T lymphocytes. Probing cosmid libraries with a MAGE-1 sequence, we identified 11 closely related genes. The analysis of hamster-human somatic cell hybrids indicated that the 12 MAGE ...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF01246677
更新日期:1994-01-01 00:00:00
abstract::Over a period of some 20 years, Werner Eugen Mayer played a significant role in establishing a framework for molecular studies of Mhc genes in multiple vertebrates. His work largely concerned gene isolation, sequencing, and related bioinformatic analyses both for the Mhc and for immune system genes of about 200 specie...
journal_title:Immunogenetics
pub_type: 传,历史文章,杂志文章,评审
doi:10.1007/s00251-012-0654-9
更新日期:2012-12-01 00:00:00
abstract::Human leucocyte antigen (HLA)-DRB1*15 is associated with predisposition to multiple sclerosis (MS), although conjecture surrounds the possible involvement of an alternate risk locus in the class I region of the HLA complex. We have shown previously that an alternate MS risk allele(s) may be encompassed by the telomeri...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-006-0183-5
更新日期:2007-03-01 00:00:00
abstract::A large number of HLA-Cw4 (Cw *0402) peptides were purified, sequenced, and identified from breast and ovarian carcinoma cell lines. HLA-Cw4 molecules were expressed in these cells as soluble, secreted HLA (sHLA) and recovered from the growth medium. The peptides were separated by capillary reversed-phase HPLC and ana...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-003-0570-0
更新日期:2003-06-01 00:00:00
abstract::The emergence in Camelidae species of functional antibodies devoid of light chains (referred to as heavy-chain antibodies or HCAbs) is an intriguing evolutionary event. Homodimeric HCAbs have also been documented in spotted ratfish ( Cos5-Abs) and nurse shark (NAR). To reveal the evolutionary history of HCAbs, we eval...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-002-0433-0
更新日期:2002-04-01 00:00:00
abstract::Severe combined immune deficiency (scid) mice are assumed to have two types of abnormalities: one is high radiosensitivity and the other is abnormal recombination in immunoglobulin and T-cell receptor genes. The human chromosome 8 q1.1 region has an ability to complement the scid aberrations. Moreover, the localizatio...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s002510050159
更新日期:1996-01-01 00:00:00
abstract::Mouse lymphocyte alloantigens Ly-19 and Ly-32 are controlled by the genes tightly linked to the Lyb-2 locus on chromosome 4. Despite the similarity in mouse strain distribution patterns, Ly-19 and Ly-32 antigens which have been detected on both B- and T-cell lineages are distinct from Lyb-2 antigen whose expression is...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00364229
更新日期:1988-01-01 00:00:00
abstract::Kinetics and family transmission of antigen-specific in vitro cell-mediated responses were investigated in 68, and serum-antibody responses to tetanus toxoid (TT) in 73 individuals from a total of 12 families. Proliferative responses to highly purified TT monomer were studied in 6- to 7-day lymphocyte cultures. The ef...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00372467
更新日期:1983-01-01 00:00:00
abstract::In the present study, we investigate patterns of variation in the KIR cluster in a large and well-characterized sample of worldwide human populations in the Human Genome Diversity Project-Centre d'Etude du Polymorphisme Humain (HGDP-CEPH) panel in order to better understand the patterns of diversity in the region. Com...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-012-0629-x
更新日期:2012-10-01 00:00:00
abstract::Footpad swelling response was used to measure the alloantigenicity of epidermal cells (ECs) in delayed-type hypersensitivity (DTH). Strong footpad swelling was observed 3 h after the challenge, and it continued for 48 h after the challenge. Genetical incompatibility between the recipients and the ECs was required to i...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00372666
更新日期:1986-01-01 00:00:00
abstract::TAP1 and TAP2 are two major histocompatibility complex (MHC) genes, located between HLA-DP and -DQ, whose products form a transporter molecule involved in endogenous antigen processing. Polymorphic residues have been described in both genes and, in this study, we have identified another polymorphic site within the ade...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00216802
更新日期:1993-01-01 00:00:00
abstract::HLA-B and C are related class I genes which are believed to have arisen by duplication of a common ancestor. Previous study showed the presence of orthologues for both HLA-B and C in African apes but only for HLA-B in Asian apes. These observations suggested that the primate C locus evolved subsequent to the divergenc...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s002510050566
更新日期:1999-09-01 00:00:00
abstract::A strong correlation between the TT(-889) genotype over CC(-889) of the interleukin-1 alpha (IL-1 alpha) transcription regulatory region and age of Alzheimer's disease onset has recently been reported. To determine the functional effects of the genetic variants on plasma protein levels, we cloned the promoter region a...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-002-0445-9
更新日期:2002-05-01 00:00:00
abstract::The vertebrate complement system is composed of about 30 serum and cell surface proteins that make up three activation pathways, a lytic pathway, and a set of proteins that regulate complement. Regulatory proteins are required for host protection against autologous complement attack and to control the amplification fe...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-004-0665-2
更新日期:2004-05-01 00:00:00
abstract::We have isolated and characterized cDNAs and genes for pufferfish, Fugu rubripes, (Fugu) orthologues of mammalian interleukin (IL)-12 subunits (IL-12 p35 and IL-12 p40). The deduced amino acid sequences of the Fugu IL-12 subunits showed homology with mammalian IL-12 subunits (p35: 50.4-58.0% similarity; p40: 51.2-55.4...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-003-0582-9
更新日期:2003-08-01 00:00:00
abstract::The potential association between integration or deletion of mouse mammary tumor virus (MMTV) retroviral sequences and the appearance of non-H-2 histocompatibility (H) antigen mutations was investigated. Genomic blots from inbred strains carrying 22 loss, gain-loss, and gain mutations on the BALB/c and C57BL/6 backgro...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00345461
更新日期:1987-01-01 00:00:00
abstract::The inbred strain STS/A exhibits a higher proliferative response in the mixed lymphocyte culture (MLC) to stimulator cells of all 11 tested inbred mouse strains with 10 different major histocompatibility complex (MHC) haplotypes, as well as to stimulation with IL-2 than does the strain BALB/cHeA. However, alloantigen-...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF02602654
更新日期:1996-01-01 00:00:00
abstract::A graft-vs-host reaction (GVHR) develops after major histocompatibility complex (MHC)-compatible bone marrow-transplantation. In the genetic combination studied, B10.D2 donor cells differed from those of (DBA/2 x B10.D2)F1 mice for multiple DBA/2 minor histocompatibility antigens (mHAg) and minor lymphocyte stimulatin...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00241258
更新日期:1994-01-01 00:00:00
abstract::An I-J-subregion controlled determinant is expressed on Ly-1 inducer and Ly-1,2 acceptor T cells in the feedback suppression circuit. Ly-1 T cells absorb the I-J antibody reactive with the Ly-1,2 acceptor T cell, suggesting that both inducer and acceptor T cells have the same I-J determinant. Since less than 10 percen...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF01567824
更新日期:1980-01-01 00:00:00