Abstract:
:Indirect immunofluorescence cytochemistry of ovine corticotropin releasing factor (oCRF) was performed by use of an antiserum raised against a conjugate of oCRF and bovine thyroglobulin. The staining intensity was quantitated by use of an automated microfluorimeter. In cryostat sections of formaldehyde fixed oCRF containing gelatine models, the staining intensity was dependent on the concentration of oCRF (1-100 microM) added to the gel. Immunoinhibition experiments showed that oCRF induced identical concentration-dependent (0.001-1 microM) quenching of the immunostaining of oCRF containing models and rat median eminence (ME) preparations. Comparison of immunoinhibition of oCRF and ME extracts indicates that approximately 1.5 ng of CRF immunoreactivity (CRFi) is present in the ME of intact adult male Wistar rats. In the hypothalamus of rats, the majority of CRFi nerve fibers are localized in the external zone of the median eminence, whereas a large population of CRFi cell bodies is present in the paraventricular nucleus. Manipulations of the pituitary-adrenal system result in changes in the distribution of CRFi in these neurons. One week after extirpation of the adrenals or of the pituitary gland, the CRFi in the ME was reduced to 32 +/- 3% and 48 +/- 6% respectively. This decrease in CRFi in the median eminence can be largely prevented by treatment of rats with dexamethasone in doses that effectively reduce plasma ACTH and corticosterone levels. In contrast to intact rats, CRFi cell bodies can be visualized in the paraventricular nucleus of non-colchicine-treated rats after adrenalectomy or hypophysectomy. These data support the view that the hypothalamic CRFi neurons play a central role in the control of pituitary-adrenal activity.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Brain Resjournal_title
Brain researchauthors
Schipper J,Werkman TR,Tilders FJdoi
10.1016/0006-8993(84)91458-6subject
Has Abstractpub_date
1984-02-13 00:00:00pages
111-8issue
1eissn
0006-8993issn
1872-6240pii
0006-8993(84)91458-6journal_volume
293pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::Stimuli such as noise or hypoxia can induce a release of ATP into the cochlear fluid spaces. At nanomolar concentrations, ATP affects neurotransmission and electrochemical regulation of sound transduction. At higher concentrations, ATP may exert cytotoxicity acting on specific P2X(7) receptor subunits, thus contributi...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2006.05.094
更新日期:2006-08-09 00:00:00
abstract::The connectivity of the stellate ganglion of Octopus was investigated using cobalt and horseradish peroxidase (HRP) tracers applied to severed preganglionic and postganglionic nerves. Centripetal cells, defined as cells in the ganglion which send their axons back toward the brain, were discovered by both methods. Appl...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(80)90583-1
更新日期:1980-02-17 00:00:00
abstract::The effects of acute ethanol exposure of chick spinal cord neurons were studied in tissue culture, using whole-cell voltage-clamp techniques. Results indicate that ethanol produces a persistent increase in the sensitivity of spinal neurons to GABA and glycine, with no change in input resistance or resting membrane pot...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(88)90098-4
更新日期:1988-07-12 00:00:00
abstract::Phospholipid extracts from 48 intracranial tumors were analyzed using 31P NMR. Phospholipids commonly identified in the tumor spectra included phosphatidylglycerol (PG), phosphatidic acid (PA), diphosphatidylglycerol (DPG), uncharacterized phospholipid (U), ethanolamine plasmalogen (EPLAS), phosphatidylethanolamine (P...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)91041-3
更新日期:1994-06-27 00:00:00
abstract::The effects of acute opiate receptor stimulation on regional cerebral oxygen consumption and blood flow were examined in 9 regions of the artificially respired, alpha-chloralose-anesthetized cat. Regional cerebral arterial and venous oxygen saturation were examined microspectrophotometrically and regional cerebral blo...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(84)91262-9
更新日期:1984-01-23 00:00:00
abstract:UNLABELLED:Mating in rabbits lasts only 3-5s but profoundly changes the female׳s physiology and behavior (e.g., inhibition of scent-marking and ambulation, changes in EEG, and release of GnRH). The behavioral responsiveness to copulation is reduced in lactating rabbits, relative to estrous does, but is enhanced after s...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2015.02.033
更新日期:2015-05-22 00:00:00
abstract::The effects of cocaine administration and withdrawal on D1 and D2 dopamine receptor number, affinity, and mRNA levels were examined in rats trained to self-administer cocaine for four weeks on a continuous reinforcement schedule. Two hours after the last infusion of cocaine there was a decrease in the number and agoni...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)90255-0
更新日期:1994-01-14 00:00:00
abstract::The release of [3H]acetylcholine (ACh) from 6-, 8-, 10-, 12-, 14- and 16-day chick embryo neural retina layers was determined using a perfusion system to collect the released neurotransmitter. After labeling the tissue with [3H]choline, samples of [3H]ACh released before and after stimulation by 80 mM KCl or 5 mM glut...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0165-3806(82)90137-7
更新日期:1982-07-01 00:00:00
abstract::The dmy rat is an autosomal recessive mutant that exhibits severe myelin destruction throughout the white matter of the central nervous system. Recently, a point mutation in intron 3 of the Mrs2 has been found in the dmy rat. Mrs2 encodes an essential component of the major electrophoretic Mg(2+) influx system in mito...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2011.03.009
更新日期:2011-05-10 00:00:00
abstract::Nitric oxide (NO) is a putative neuronal messenger in the olfactory epithelium. We analysed the electrical responses of rat olfactory receptor neurones to NO stimulation in the whole-cell patch clamp mode and found that NO activates the cyclic nucleotide-gated conductance, independent from cyclic GMP. As NO may also o...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(03)02990-1
更新日期:2003-08-01 00:00:00
abstract::Brain serotonin (5-HT) uptake sites were quantitated, by saturation binding of [3H]paroxetine, in 10 brain regions from 22 suicide victims and 20 control subjects. Suicide victims were restricted to those subjects in whom a firm retrospective diagnosis of depression was established and who had not recently been prescr...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(90)90244-6
更新日期:1990-08-27 00:00:00
abstract:OBJECTIVE:The modern brain imaging studies in patients with eating disorders have shown that neurotransmitting regulation differs distinctly from control groups. These disturbances have involved serotonin and dopamine system can be inherited and conditioned by genes. The aim of this work has been the analysis of associ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2009.10.035
更新日期:2010-01-11 00:00:00
abstract::Distribution and time course of the occurrence of "dark" neurons were compared with the EEG activity and behavior of rats during 4-aminopyridine (4-AP) induced epileptic seizures. A crystal of the K(+) channel blocker 4-AP (0.5 mg/kg) was placed onto the exposed parieto-occipital cortex of Halothane-anesthetized rats ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2008.06.044
更新日期:2008-09-04 00:00:00
abstract::This study determined baroreceptor reflex (BR) function in conscious rats which had received sham or electrolytic lesions of the anteroventral third ventricle (AV3V) 54-56 days previously. Resting mean arterial pressure (MAP) and heart rate (HR) values of the AV3V-lesion rats were similar to those of sham-lesion rats ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(99)01561-9
更新日期:1999-07-24 00:00:00
abstract::This study was performed to investigate the mechanism how amantadine increases the extracellular dopamine (DA) levels in the striatum in vivo. Local application of amantadine (1 mM, 40 min) to the striatum through the dialysis membrane significantly increased the extracellular DA levels. Coadministration of nomifensin...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)90821-4
更新日期:1994-10-31 00:00:00
abstract::The serotoninergic (5-hydroxytryptamine, 5-HT) innervation of the rat ventral tegmental area (VTA) was examined by light and electron microscopic radioautography following intraventricular infusion of [3H]5-HT. The [3H]5-HT labeled processes were characterized with respect to their regional distribution, ultrastructur...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(87)91588-5
更新日期:1987-12-01 00:00:00
abstract::The stimulated (40 Hz) release of enkephalin from the myenteric plexus can be modulated by multiple types of opiate receptor. The direction of the modulation is not fixed but is bimodal. Both an inhibition and an enhancement of evoked release is observed depending on the concentration of opioid agonist that is used. E...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(89)91594-1
更新日期:1989-12-11 00:00:00
abstract::We studied seizures which were induced by a microinjection of kainic acid (KA) into the unilateral olfactory bulb (OB) in the rats. The first epileptiform discharge appeared in the OB, was then propagated to the amygdala and the hippocampus unilaterally, and finally propagated to the unilateral sensori-motor cortex. C...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(95)00626-2
更新日期:1995-09-25 00:00:00
abstract::The parafascicular thalamic nucleus (nPf) is a critical relay in the ascending system that mediates motor control in the central nervous system (CNS). Yet, little is known about whether or not the nPf is involved in the development of morphine dependence and withdrawal. In the present study, kainic acid was used to ch...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2009.02.084
更新日期:2009-05-19 00:00:00
abstract::The role of calcium and protein kinases in rhythmic activity induced by muscarinic receptor activation in the CA1 area in rat hippocampal slices was investigated. Extracellular recording showed that carbachol (20 microM) induced synchronized field potential activity with a dominant frequency of 7.39+/-0.68 Hz. Pretrea...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(00)02331-3
更新日期:2000-07-28 00:00:00
abstract:BACKGROUND:Hypoxia is a critical secondary injury mechanism in traumatic brain injury (TBI), and early intervention to alleviate post-TBI hypoxia may be beneficial. NVX-108, a dodecafluoropentane perfluorocarbon, was screened for its ability to increase brain tissue oxygen tension (PbtO2) when administered soon after T...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2016.01.012
更新日期:2016-03-01 00:00:00
abstract::The immunocytological study of LH-RH producing neurons was carried out on 3 newborns and 14 fetuses (from 10 to 36 weeks of age). Perikarya and fibers which were immunoreactive to anti-LH-RH IS were revealed by IF or IE in all the hypothalamus beginning with the 13th week. Important variations in neurons staining may ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(77)90992-1
更新日期:1977-06-10 00:00:00
abstract::Fetal exposure to dexamethasone (DEX) alters brain plasticity and cognitive functions during adulthood in a sex-dependent manner. The mechanisms underlying such long-lasting sex-dependent change of prenatal DEX is not well understood. The p73 gene plays an important role in brain development. It encodes for two protei...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2018.11.030
更新日期:2019-03-15 00:00:00
abstract::In anesthetized rats, out of 48 paraventricular (PaV) and 19 periventricular (PeV) neurosecretory cells, 12 PaV and 6 PeV cells increased and 10 PaV cells decreased the electrical activities statically in response to preoptic and anterior hypothalamic warming. Four PaV and 2 PeV cells responded dynamically to the ther...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(83)90036-7
更新日期:1983-12-19 00:00:00
abstract::We previously showed indirectly that the increase in diacylglycerol (DAG) levels caused by exposing differentiating PC12 cells to nerve growth factor (NGF) must derive mainly from de novo synthesis and, to a lesser and transient extent, from the hydrolysis of [3H]phosphatidylinositol (PI). To explore further the bioch...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(98)01280-3
更新日期:1999-02-13 00:00:00
abstract::Immunofluorescence on primary dissociated rat neuronal cultures (cortical, hippocampal, and cerebellar) and organotypic hippocampal cultures was used to investigate the pattern of distribution of cell-surface amyloid protein (APP). Antibodies directed against the extracellular (N-terminal) portion of APP or against th...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(96)00608-7
更新日期:1996-10-07 00:00:00
abstract::Within the bullfrog semicircular canal crista, hair cell tuft types were defined and mapped with the aid of scanning electron microscopy. Intracellular recording and Lucifer Yellow labeling techniques were used to study afferent responses and arborization patterns. Dye-filled planar afferent axons had mean distal axon...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(90)90107-m
更新日期:1990-11-26 00:00:00
abstract::Brain edema is a serious consequence of hemispheric stroke and traumatic brain injury and contributes significantly to patient mortality. In the present study, we measured water contents in hippocampal slices as an in vitro model of edema formation. Excitotoxic conditions induced by N-methyl-D-aspartate (NMDA, 300 mic...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2006.09.012
更新日期:2006-11-29 00:00:00
abstract::The central nervous system (CNS) in adult mammals loses the ability to regenerate after injury. Although electrical stimulation (ES) has been shown to promote neural regeneration, the underlying mechanisms by which ES enhances CNS regeneration remain elusive. The aim of the present study was to investigate the effect ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2012.08.041
更新日期:2012-10-22 00:00:00
abstract::Astrocytes produce and bind endothelins (ETs), suggesting that these cells have ET autoregulatory and eliminatory functions. To further investigate these functions in primary rat astrocytes, ET-1 levels in the cell culture media (RIA/HPLC) and intracellular content of ET-1 mRNA (RT PCR) were measured under basal and s...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)01368-1
更新日期:1998-03-02 00:00:00