Localization of cholecystokinin-octapeptide-like-immunoreactivity in neurons cultured from rat cerebral cortex.

Abstract:

:Cholecystokinin-octapeptide-like-immunoreactivity (CCK-8-LI) is demonstrable by the indirect immunofluorescent technique in cerebral cortical neurons cultured from fetal rats. Prominent and widespread staining is seen in widely ramifying cellular processes throughout the cultures. Staining in neuronal cell bodies is enhanced by treatment of the cultures with colchicine prior to fixation and can be seen to extend from the perikaryal region into dendrites and axonal processes. While several morphological types of neurons contain CCK-8-LI, there is a relative preponderance of small and moderate-sized bipolar and, to a lesser extent, multipolar neurons. These findings are in concordance with the findings of several investigators in studies of brain sections. The further finding of frequent groupings of CCK-8 neurons is noted and discussed.

journal_name

Brain Res

journal_title

Brain research

authors

Delfs JR,Zhu CH,Dichter MA

doi

10.1016/0006-8993(84)91456-2

subject

Has Abstract

pub_date

1984-02-13 00:00:00

pages

93-9

issue

1

eissn

0006-8993

issn

1872-6240

pii

0006-8993(84)91456-2

journal_volume

293

pub_type

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