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Receptor binding and biological effect of insulin in human adipocytes.

Abstract：

:The binding of 125I-labelled insulin to human adipocytes was studied at 37 degrees C. The precipitability of the 125I-labelled insulin preparation (0.03 nmol/l) in trichloroacetic acid and the concentration of biologically active insulin (7.5 nmol/l) remained constant in buffer incubated with human adipocytes (100 microliter cells/ml suspension) for 30--60 minutes at 37 degrees C, whereas more than half of the insulin was inactivated by rat fat cells under the same conditions. A constant level of binding of 125I-labelled insulin (0.03 nmol/l) to human adipocytes was obtained after 45 minutes. The apparent dissociation constant of receptor binding was about 0.2 nmol/l as compared to about 2 nmol/l for rat adipocytes. Conversion of [UP14C]glucose to lipids was stimulated half-maximally by about 0.05 nmol/l of insulin (similar to rat adipocytes). Thus, half-maximal stimulation of human adipocytes was obtained with a recptor occupancy of about 20--30 per cent.

journal_name

Diabetologia

journal_title

Diabetologia

authors

Andersen O,Gliemann J,Gammeltoft S

doi

10.1007/BF01236312

subject

Has Abstract

pub_date

1977-12-01 00:00:00

pages

589-93

issue

6

eissn

0012-186X

issn

1432-0428

journal_volume

13

pub_type

杂志文章

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