Abstract:
AIMS/HYPOTHESIS:The therapeutic benefit of physical activity to prevent and treat type 2 diabetes is commonly accepted. However, the impact of the disease on the acute metabolic response is less clear. To this end, we investigated the effect of type 2 diabetes on exercise-induced plasma metabolite changes and the muscular transcriptional response using a complementary metabolomics/transcriptomics approach. METHODS:We analysed 139 plasma metabolites and hormones at nine time points, and whole genome expression in skeletal muscle at three time points, during a 60 min bicycle ergometer exercise and a 180 min recovery phase in type 2 diabetic patients and healthy controls matched for age, percentage body fat and maximal oxygen consumption (VO2). RESULTS:Pathway analysis of differentially regulated genes upon exercise revealed upregulation of regulators of GLUT4 (SLC2A4RG, FLOT1, EXOC7, RAB13, RABGAP1 and CBLB), glycolysis (HK2, PFKFB1, PFKFB3, PFKM, FBP2 and LDHA) and insulin signal mediators in diabetic participants compared with controls. Notably, diabetic participants had normalised rates of lactate and insulin levels, and of glucose appearance and disappearance, after exercise. They also showed an exercise-induced compensatory regulation of genes involved in biosynthesis and metabolism of amino acids (PSPH, GATM, NOS1 and GLDC), which responded to differences in the amino acid profile (consistently lower plasma levels of glycine, cysteine and arginine). Markers of fat oxidation (acylcarnitines) and lipolysis (glycerol) did not indicate impaired metabolic flexibility during exercise in diabetic participants. CONCLUSIONS/INTERPRETATION:Type 2 diabetic individuals showed specific exercise-regulated gene expression. These data provide novel insight into potential mechanisms to ameliorate the disturbed glucose and amino acid metabolism associated with type 2 diabetes.
journal_name
Diabetologiajournal_title
Diabetologiaauthors
Hansen JS,Zhao X,Irmler M,Liu X,Hoene M,Scheler M,Li Y,Beckers J,Hrabĕ de Angelis M,Häring HU,Pedersen BK,Lehmann R,Xu G,Plomgaard P,Weigert Cdoi
10.1007/s00125-015-3584-xsubject
Has Abstractpub_date
2015-08-01 00:00:00pages
1845-54issue
8eissn
0012-186Xissn
1432-0428journal_volume
58pub_type
杂志文章相关文献
DIABETOLOGIA文献大全abstract:AIMS/HYPOTHESIS:Mesenchymal stem cells (MSCs) have been shown to abrogate in vitro the proinflammatory response in type 1 diabetes. The mechanism involves paracrine factors, which may include microvesicles (MVs). We evaluated whether MVs derived from heterologous bone-marrow MSCs exert an immunomodulatory effect on T c...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-014-3262-4
更新日期:2014-08-01 00:00:00
abstract:AIMS/HYPOTHESIS:It has been recently shown that glycated human haemoglobin induces endothelial dysfunction in rat vessels by generating superoxide anions that interfere with nitric oxide mediated responses. Our study analysed the effect of glycated human haemoglobin on the endothelium-dependent relaxations of human ves...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s001250050011
更新日期:2000-01-01 00:00:00
abstract:AIMS/HYPOTHESIS:Hyperlipidaemia is an independent risk factor for the progression of diabetic nephropathy, but its molecular mechanism remains elusive. We investigated in mice how diabetes and hyperlipidaemia cause renal lesions separately and in combination, and the involvement of Toll-like receptor 4 (TLR4) in the pr...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-012-2578-1
更新日期:2012-08-01 00:00:00
abstract::The validity of the new diagnostic criteria for diabetes mellitus for Japanese subjects was assessed by the long-term prognosis of 501 patients, found in an epidemiological survey of 6,681 people in a Japanese town. Ten-year relative survival rates were examined in relation to the 50 g oral glucose tolerance test (ser...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00252627
更新日期:1981-03-01 00:00:00
abstract::Lack of insulin receptors in mice is associated with near-normal intrauterine growth, unlike patients with leprechaunism, in whom growth deficiency is a prominent clinical feature. Genetic crosses of insulin receptor- and insulin-like growth-factor-1 (IGF-1) receptor-deficient mice indicate, however, that insulin rece...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s001250050886
更新日期:1998-02-01 00:00:00
abstract:AIMS/HYPOTHESIS:Insulin-mediated glucose transport and utilisation are decreased in skeletal muscle from type 2 diabetic and glucose-intolerant individuals because of alterations in insulin receptor signalling, GLUT4 translocation to the plasma membrane and microvascular blood flow. Catalytic activity of the muscle-spe...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-013-3084-9
更新日期:2014-01-01 00:00:00
abstract:AIMS/HYPOTHESIS:Hyperglycaemia in diabetes is associated with increased glycation, oxidative stress and nitrosative stress. Proteins modified consequently contain glycation, oxidation and nitration adduct residues, and undergo cellular proteolysis with release of corresponding free adducts. These free adducts leak into...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-005-1810-7
更新日期:2005-08-01 00:00:00
abstract::Recent studies have suggested an association between a deletion (D) variant of the angiotensin-converting-enzyme (ACE) gene and diabetic nephropathy. However, this finding has not been confirmed by all investigators. Furthermore, an M235T variant of the angiotensinogen (AGT) gene has been associated with hypertension,...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s001250050662
更新日期:1997-02-01 00:00:00
abstract:AIMS/HYPOTHESIS:We hypothesised that type 1 diabetic patients with established diabetic sensorimotor polyneuropathy (DSPN) would have segmental and/or pan-enteric dysmotility in comparison to healthy age-matched controls. We aimed to investigate the co-relationships between gastrointestinal function, degree of DSPN and...
journal_title:Diabetologia
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00125-016-4199-6
更新日期:2017-04-01 00:00:00
abstract::To examine differences among pregnant diabetic and nondiabetic subjects, serum glucose, and immunoreactivity of C-peptide, free and total insulin were measured at hourly intervals during a 24--h third trimester metabolic ward evaluation. Six normals, three mild, and four juvenile-onset type diabetics were studied. Die...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00420978
更新日期:1976-08-01 00:00:00
abstract::The primary goal of the present study was to examine the effects of improved glycaemic control associated with glipizide treatment on postprandial lipaemia in non-insulin-dependent diabetic patients. The metabolism of triglyceride-rich lipoproteins of intestinal origin was assessed by measuring the retinyl palmitate c...
journal_title:Diabetologia
pub_type: 临床试验,杂志文章
doi:10.1007/BF00404335
更新日期:1994-08-01 00:00:00
abstract:AIMS/HYPOTHESIS:Intensive glucose control reduces the risk of vascular complications while increasing the risk of severe hypoglycaemia at a group level. We sought to estimate individual beneficial and adverse effects of intensive glucose control in patients with type 2 diabetes. METHODS:We performed a post hoc analysi...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-016-4082-5
更新日期:2016-12-01 00:00:00
abstract:AIMS/HYPOTHESIS:Maternal Type I (insulin-dependent) diabetes mellitus is associated with an increased risk for fetal malformations and spontaneous abortions. Although the pathogenic mechanism is not fully understood, reactive oxygen species have been shown to contribute to the pathogenesis in experimental studies. By m...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s001250051686
更新日期:2001-06-01 00:00:00
abstract:AIMS/HYPOTHESIS:Circulating fibroblast growth factor 21 (FGF21) levels are often elevated in obesity, dyslipidaemia, insulin resistance and type 2 diabetes. This study investigated the relationship of plasma FGF21 levels with cardiovascular events in patients with type 2 diabetes. METHODS:Plasma FGF21 levels were meas...
journal_title:Diabetologia
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00125-014-3458-7
更新日期:2015-03-01 00:00:00
abstract:AIMS/HYPOTHESIS:Mitochondrial oxidative phosphorylation (OXPHOS) plays an important role in the pathophysiology of type 2 diabetes. Genes involved in OXPHOS have been reported to be down-regulated in skeletal muscle from patients with type 2 diabetes; however, hepatic regulation is unknown. MATERIALS AND METHODS:We an...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-006-0489-8
更新日期:2007-02-01 00:00:00
abstract::Multiple epidemiological studies have documented an association between metformin, used for treatment of type 2 diabetes, and reduced cancer incidence and mortality. Cell line models may not accurately reflect the effects of metformin in the clinical setting. Moreover, findings from animal model studies have been inco...
journal_title:Diabetologia
pub_type: 杂志文章,评审
doi:10.1007/s00125-017-4372-6
更新日期:2017-09-01 00:00:00
abstract:AIMS/HYPOTHESIS:Our aims were to compare osteoclastic activity between patients with acute Charcot's osteoarthropathy and diabetic and healthy controls, and to determine the effect of the receptor activator of nuclear factor-kappaB ligand (RANKL) and its decoy receptor osteoprotegerin (OPG). METHODS:Peripheral blood m...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-008-0992-1
更新日期:2008-06-01 00:00:00
abstract:AIMS/HYPOTHESIS:High sodium (HS) effects on hypertension are well established. Recent evidence implicates a relationship between HS intake and insulin resistance, even in the absence of hypertension. The aim of the current study was to determine whether loss of the vascular actions of insulin may be the driving factor ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-014-3373-y
更新日期:2014-12-01 00:00:00
abstract::Short- and long-term regulation of hepatic carbohydrate metabolism by insulin-like growth factor II was studied in primary cultures of adult rat hepatocytes and compared to the metabolic potency of insulin. Insulin-like growth factor II stimulated glycogen synthesis from [14C]glucose, uptake of [3H]aminoisobutyric aci...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00400920
更新日期:1992-03-01 00:00:00
abstract::The development of proteinuria and its response to insulin therapy in acute experimental diabetes was examined in 11 female rats by sequentially measuring 24 h excretion of glucose and total protein before diabetes, during 5-37 days of acute streptozotocin diabetes, during and after 7-14 days of continuous subcutaneou...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF03216225
更新日期:1981-07-01 00:00:00
abstract::To study the effect of E. Coli L-asparaginase on glucose tolerance and insulin release, 6 patients with neoplastic disease were subjected to 3 hour oral glucose tolerance tests with simultaneous measurement of serum immunoreactive insulin (IRI) levels before and following the intravenous administration of 5000 I. U. L...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00422255
更新日期:1978-08-01 00:00:00
abstract::To determine whether increased oxidative stress in diabetes mellitus is due to an impaired free-radical scavenger function in endothelial cells, GSH-dependent H2O2 degradation in human umbilical vein endothelial cells was studied. The GSH-dependent, NaN3-uninhibitable H2O2-degradation in endothelial cells was reduced ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00398053
更新日期:1994-03-01 00:00:00
abstract::This study aimed at evaluating the influence of furosemide upon insulin and glucagon responses to arginine in healthy subjects. For this purpose, six normal subjects received two consecutive arginine pulses (3 g), 60 min apart, before and after the administration of furosemide (40 mg, IV). The acute insulin response (...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00251008
更新日期:1980-04-01 00:00:00
abstract::The effect of hyperglycaemia on islet transplantation in the rat has been examined in two ways, using syngeneic transplantation of 400 islets to the kidney capsule and subsequent measurement of kidney insulin content as a measurement of B-cell survival. Firstly, islets were transplanted into either diabetic or normal ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00274254
更新日期:1989-09-01 00:00:00
abstract::Type 1 diabetes is an immune-mediated disease leading to almost total beta cell destruction and permanent exogenous insulin dependency. The appearance of clinical symptoms is preceded by an asymptomatic preclinical period, the duration of which is highly individual. The emergence of diabetes-associated autoantibodies ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-016-4147-5
更新日期:2017-01-01 00:00:00
abstract:AIMS/HYPOTHESIS:Abnormal gut microbiota and blood metabolome profiles have been reported both in children and adults with uncomplicated type 1 diabetes as well as in adults with type 1 diabetes and advanced stages of diabetic nephropathy. In this study we aimed to investigate the gut microbiota and a panel of targeted ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-020-05260-y
更新日期:2020-12-01 00:00:00
abstract:AIMS/HYPOTHESIS:Gestational diabetes mellitus (GDM) is a risk factor for perinatal complications. In several countries, the criteria for the diagnosis of GDM have been in flux, the American Diabetes Association (ADA) thresholds recommended in 2000 being lower than those of the National Diabetes Data Group (NDDG) that h...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-006-0517-8
更新日期:2007-02-01 00:00:00
abstract:AIMS/HYPOTHESIS:We hypothesised that non-obese diabetic mice (NOD) mice have an autoimmune-mediated loss of islet sympathetic nerves and an impairment of sympathetically mediated glucagon responses. We aimed: (1) to determine whether diabetic NOD mice have an early impairment of the glucagon response to insulin-induced...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-009-1494-5
更新日期:2009-12-01 00:00:00
abstract::We have investigated whether and how the elevated glucose concentrations characteristic of diabetes may alter the interaction of endothelial cells with low-density lipoproteins (LDL). Protracted exposure of cultured human endothelial cells to 20 mmol/l glucose failed to affect either the relationship between the degre...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00252411
更新日期:1984-03-01 00:00:00
abstract:AIMS/HYPOTHESIS:The aim of this study was to examine human enteroviruses (HEVs) and other intestinal viruses derived from children who participated in the Babydiet intervention study and to analyse the findings according to the appearance of islet autoantibodies, dietary intervention, maternal type 1 diabetes and clini...
journal_title:Diabetologia
pub_type: 临床试验,杂志文章
doi:10.1007/s00125-011-2305-3
更新日期:2011-12-01 00:00:00