Abstract:
:We have investigated whether and how the elevated glucose concentrations characteristic of diabetes may alter the interaction of endothelial cells with low-density lipoproteins (LDL). Protracted exposure of cultured human endothelial cells to 20 mmol/l glucose failed to affect either the relationship between the degree of confluency of the monolayer and the extent of LDL degradation or the dose-responses for LDL uptake and degradation. In contrast, non-enzymatic glycosylation of LDL by pre-incubation of LDL with glucose markedly inhibited their uptake and degradation by endothelial cells. Thus, at protein concentration of 5 micrograms/ml, the amount of glycosylated 125I-LDL associated with cells was decreased fourfold compared with native 125I-LDL (47 +/- 3 versus 194 +/- 10 ng X mg cell protein-1 X 24 h-1, mean +/- SEM), and degradation was decreased twenty-fold (135 +/- 4 versus 2873 +/- 115 ng X mg cell protein-1 X 24 h-1). The degree of inhibition was proportional to the extent of glycosylation. At all concentrations studied, methylated LDL behaved similarly to glycosylated LDL. The decreased recognition of glycosylated LDL by the endothelial lining of small and large blood vessels may have an impact of tissue physiology and on the overall fate of the glycosylated molecules.
journal_name
Diabetologiajournal_title
Diabetologiaauthors
Lorenzi M,Cagliero E,Markey B,Henriksen T,Witztum JL,Sampietro Tdoi
10.1007/BF00252411subject
Has Abstractpub_date
1984-03-01 00:00:00pages
218-22issue
3eissn
0012-186Xissn
1432-0428journal_volume
26pub_type
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