Abstract:
:The selective permeability of alginate microcapsules, containing isolated rat islets of Langerhans or insulin secreting RINm5F cells, was investigated in vitro. An increase in insulin release was observed when microencapsulated islets were stimulated by glucose+theophylline, and when microencapsulated RINm5F cells were stimulated by arginine+theophylline. These findings demonstrate the permeability of the microcapsule membrane to these B-cell secretagogues and to insulin. Immunoisolation of RINm5F cells by microencapsulation was assessed using a 51chromium cytotoxicity test. Significant 51Cr release was observed when nonencapsulated cells were incubated with complement and either the serum of a rabbit immunized with RIN cells or the sera of two patients with recently diagnosed Type 1 (insulin-dependent) diabetes. This effect was not observed with encapsulated cells. Both free and encapsulated cells released 80% of their initial radioactivity when incubated in the presence of HC1. These results clearly demonstrate pancreatic cell immunoisolation by microencapsulation. They also provide a method for the in vitro evaluation of the functional characteristics of microcapsules, in terms of both insulin permeability and immunoprotection.
journal_name
Diabetologiajournal_title
Diabetologiaauthors
Darquy S,Reach Gdoi
10.1007/BF00265027subject
Has Abstractpub_date
1985-10-01 00:00:00pages
776-80issue
10eissn
0012-186Xissn
1432-0428journal_volume
28pub_type
杂志文章相关文献
DIABETOLOGIA文献大全abstract:AIMS/HYPOTHESIS:The paucity of information on the epigenetic barriers that are blocking reprogramming protocols, and on what makes a beta cell unique, has hampered efforts to develop novel beta cell sources. Here, we aimed to identify enhancers in pancreatic islets, to understand their developmental ontologies, and to ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-012-2797-5
更新日期:2013-03-01 00:00:00
abstract::Aspects of the regulation of the glucose transport by perfused hearts of normal rats have been studied by measuring glucose transport (via the efflux of labelled 3-O-methyl-D-glucose) and glucose transporters (via the labelled cytochalasin B binding assay). Similarly to what is observed with insulin, increasing worklo...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00395557
更新日期:1988-02-01 00:00:00
abstract:AIMS/HYPOTHESIS:Diabetes promotes cerebral neovascularisation via increased vascular endothelial growth factor (VEGF) angiogenic signalling. Roundabout-4 (ROBO4) protein is an endogenous inhibitor of VEGF signalling that stabilises the vasculature. Yet, how diabetes affects ROBO4 function remains unknown. We hypothesis...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-017-4214-6
更新日期:2017-04-01 00:00:00
abstract::The nerve fibre loss, atrophy and injury of diabetic peripheral polyneuropathy and their responses to metabolic intervention have been studied by morphometric analysis of sural nerve biopsies. The magnitudes and sources of intra- and inter-individual variation in these morphometric measures have not been investigated ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00400485
更新日期:1992-06-01 00:00:00
abstract:AIMS/HYPOTHESIS:Hyperglycaemia, a key feature of diabetes, is associated with non-enzymatic glycation of plasma proteins. We have shown previously that the reactive alpha-oxoaldehyde, methylglyoxal, non-enzymatically glycates apolipoprotein (Apo)A-I, the main apolipoprotein of HDL, and prevents it from activating lecit...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-008-0986-z
更新日期:2008-06-01 00:00:00
abstract:AIMS/HYPOTHESIS:To identify the possible causes of the lengthening of the action potential duration described in patients affected by diabetes mellitus. METHODS:We studied the effects of streptozotocin-induced diabetes on the current density of the repolarising potassium currents It(o), IK, Iss and IK1 in enzymaticall...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s001250050013
更新日期:2000-01-01 00:00:00
abstract::Plasma glucose control and arterial pressure were assessed in 28 Type 1 (insulin-dependent) diabetic patients with different degrees of micro-albuminuria. They were divided into two groups according to their urinary albumin excretion rate: a low micro-albuminuria group (n = 16) with albumin excretion ranging between 1...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00262209
更新日期:1984-06-01 00:00:00
abstract:AIMS/HYPOTHESIS:In order to identify type 2 diabetes disease susceptibility gene(s) in a Japanese population, we applied a region-wide case-control association test to the 20.4 Mb region between D3S1293 and D3S2319 on chromosome 3p24.3-22.1, supported by linkage to type 2 diabetes and its related traits in Japanese and...
journal_title:Diabetologia
pub_type: 杂志文章,多中心研究
doi:10.1007/s00125-007-0631-2
更新日期:2007-06-01 00:00:00
abstract:AIMS/HYPOTHESIS:The islet microcirculation has morphological characteristics resembling those of renal glomeruli. Transcription of the nephrin gene, a highly specific barrier protein of the slit diaphragm of podocyte foot processes, has been reported in the pancreas, although its cellular localisation and function rema...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-005-1865-5
更新日期:2005-09-01 00:00:00
abstract:AIMS/HYPOTHESIS:Abnormal rheological properties of erythrocytes, leucocytes and plasma may have a role in the development of diabetic microangiopathy. We hypothesized that changed haemorrheological variables may already be found in children with onset diabetes. METHODS:Erythrocyte deformation (rheoscope), neutrophil d...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s001250051239
更新日期:1999-07-01 00:00:00
abstract:AIMS/HYPOTHESIS:Potential differences in cardiovascular risk by ethnicity remain uncertain. We evaluated the association of ethnicity with cardiovascular disease (CVD) incidence in a large cohort of people with type 2 diabetes living in Scotland. METHODS:Data from Scottish Care Information-Diabetes (SCI-Diabetes) were...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-015-3492-0
更新日期:2015-04-01 00:00:00
abstract::The intraperitoneal route may offer more physiological insulin delivery through absorption of a proportion of the dose into the portal circulation. We have compared 1-h square-wave and bolus supplemental infusions of a fixed dose of 10 U of intraperitoneal insulin in 6 C-peptide negative insulin-dependent diabetic pat...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00265019
更新日期:1985-10-01 00:00:00
abstract:AIMS/HYPOTHESIS:Mitochondrial oxidative phosphorylation (OXPHOS) plays an important role in the pathophysiology of type 2 diabetes. Genes involved in OXPHOS have been reported to be down-regulated in skeletal muscle from patients with type 2 diabetes; however, hepatic regulation is unknown. MATERIALS AND METHODS:We an...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-006-0489-8
更新日期:2007-02-01 00:00:00
abstract:AIMS/HYPOTHESIS:Glomerular matrix accumulation is a hallmark of diabetic nephropathy. Recent data have linked the serine/threonine kinase protein kinase B (Akt) to matrix modulation. Here, we studied its role in high glucose-induced collagen elaboration by mesangial cells. METHODS:Primary rat mesangial cells were trea...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-007-0721-1
更新日期:2007-09-01 00:00:00
abstract:AIMS/HYPOTHESIS:Pancreatic islet transplantation (PIT) offers a physiological treatment for type 1 diabetes, but the failure of islet engraftment hinders its application. The female hormone 17β-oestradiol (E2) favours islet survival and stimulates angiogenesis, raising the possibility that E2 may enhance islet engraftm...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-012-2764-1
更新日期:2013-02-01 00:00:00
abstract::Among monosaccharides, fructose has a small hyperglycaemic effect. In order to better explain the mechanisms which cause this metabolic property, we used tracers labelled with stable isotopes (deuterated glucose and naturally 13C labelled fructose) to quantify the overall glucose appearance, the rate of appearance in ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00400238
更新日期:1993-04-01 00:00:00
abstract::The effect of hyperglycaemia on islet transplantation in the rat has been examined in two ways, using syngeneic transplantation of 400 islets to the kidney capsule and subsequent measurement of kidney insulin content as a measurement of B-cell survival. Firstly, islets were transplanted into either diabetic or normal ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00274254
更新日期:1989-09-01 00:00:00
abstract:AIMS/HYPOTHESIS:We aimed to examine the effects of bariatric surgery on microvascular complications in patients with type 2 diabetes using objective measures. METHODS:Prospective case-control study of 70 obese surgical patients with type 2 diabetes undergoing gastric bypass surgery matched for age, sex and duration of...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-015-3595-7
更新日期:2015-07-01 00:00:00
abstract::Human crude and recombinant interleukin 1 (IL-1) was found to dose- and time-dependently affect the biosynthesis of (pro)insulin in isolated rat islets of Langerhans. Incubation of rat islets with either 0.5 U/ml or 5 U/ml of crude IL-1 for 1 h had no detectable effect on (pro)insulin biosynthesis. After 24 hours of e...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00279615
更新日期:1987-07-01 00:00:00
abstract:AIMS/HYPOTHESIS:Higher habitual coffee drinking has been associated with a lower risk of developing type 2 diabetes. The relation between coffee consumption and risk of cardiovascular disease (CVD) has been examined in many studies, but the issue remains controversial. This study was designed to assess the association ...
journal_title:Diabetologia
pub_type: 杂志文章,多中心研究
doi:10.1007/s00125-006-0435-9
更新日期:2006-11-01 00:00:00
abstract::The effect of acute lowering of arterial blood pressure upon kidney function in nephropathy was studied in 13 patients with long-term Type 1 (insulin-dependent) diabetes. Ten normal subjects (six normotensive and four hypertensive) and five short-term Type 1 diabetic patients without nephropathy served as controls. Re...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00276965
更新日期:1984-12-01 00:00:00
abstract::It has been suggested that bradykinin stimulates glucose uptake in experiments in vivo and in cultured cells. However, its mechanism has not yet been fully elucidated. In this study, the effects of bradykinin on the insulin signalling pathway were evaluated in isolated dog adipocytes. The bradykinin receptor binding s...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00400672
更新日期:1996-04-01 00:00:00
abstract::Diabetes affects 4 to 5% of the population worldwide and is the most common metabolic disorder. The number of individuals diagnosed with diabetes is rapidly increasing, especially in the developed countries and the disorder frequently leads to secondary complications such as retinopathy, nephropathy, neuropathy and ca...
journal_title:Diabetologia
pub_type: 杂志文章,评审
doi:10.1007/s001250100623
更新日期:2001-09-01 00:00:00
abstract:AIMS/HYPOTHESIS:Hypertension, obesity, impaired glucose tolerance and dyslipidaemia are metabolic abnormalities that often cluster together more often than expected by chance alone. Since these metabolic variables are highly heritable and are at least partially genetically determined, the clustering of defects in these...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-007-0720-2
更新日期:2007-08-01 00:00:00
abstract::Pancreatic beta cells differ in terms of glucose responsiveness, insulin secretion and proliferative capacity; however, the molecular pathways that regulate this cellular heterogeneity are unknown. We have identified the Wnt-planar cell polarity (PCP) effector Flattop (FLTP) as a biomarker that identifies mature beta ...
journal_title:Diabetologia
pub_type: 杂志文章,评审
doi:10.1007/s00125-016-3949-9
更新日期:2016-09-01 00:00:00
abstract::The aim of the present study was to determine whether short-term appropriate insulinization of Type 1 (insulin-dependent) diabetic patients in long-term poor glycaemic control (HbA1C > 9.5%) was associated with an adaptive regulation of the activity and gene expression of key proteins in muscle glycogen storage and gl...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00428782
更新日期:1994-01-01 00:00:00
abstract:AIMS/HYPOTHESIS:Enterovirus infections are among the environmental risk factors potentially contributing to the pathogenesis of Type 1 diabetes. The aim of this study was to evaluate virus-host interaction by analysing the enterovirus antibody levels in subjects carrying different HLA-DR alleles associated with either ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-003-1157-x
更新日期:2003-08-01 00:00:00
abstract:AIMS/HYPOTHESIS:MicroRNAs are key regulators of gene expression involved in health and disease. The goal of our study was to investigate the global changes in beta cell microRNA expression occurring in two models of obesity-associated type 2 diabetes and to assess their potential contribution to the development of the ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-013-2993-y
更新日期:2013-10-01 00:00:00
abstract:AIMS/HYPOTHESIS:The abnormal intrauterine milieu of intrauterine growth retardation (IUGR) permanently alters gene expression and function of pancreatic beta cells leading to the development of diabetes in adulthood. Expression of the pancreatic homeobox transcription factor Pdx1 is permanently reduced in IUGR islets s...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-011-2250-1
更新日期:2011-10-01 00:00:00
abstract::Blood glucose values close to normal reduce the microvascular complications of insulin-dependent diabetes mellitus. The Stockholm study of this effect continued after the initial 7.5-year period in order to see what happened when intensively treated patients were left to control their own treatment while treatment was...
journal_title:Diabetologia
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s001250050602
更新日期:1996-12-01 00:00:00