Abstract:
:The reactions of lectins with dissociated and cultured epithelial cells from hyperplastic lenses of two unrelated chick strains, Hy-1 and Hy-2, characterised by hyperplasia of the lens epithelium, and lenses of a normal genotype (N) were investigated using four different lectins. Three methods of monitoring lectin-binding to cell surfaces were employed. Each of the four lectins used showed an individual pattern of reactivity to separated membrane components. Data obtained with the three labelling methods showed the same trend viz: increased agglutinability and high affinity for binding of all four lectins by Hy-1 and Hy-2 cells. These results suggest that Hy-1 and Hy-2 lens epithelial cells are characterised by alteration in their cell surfaces.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Odeigah PG,Clayton RM,Truman DEdoi
10.3109/02713688509000845subject
Has Abstractpub_date
1985-03-01 00:00:00pages
161-8issue
3eissn
0271-3683issn
1460-2202journal_volume
4pub_type
杂志文章abstract::To better define the growth requirements for corneal epithelial cells, methods for serum-free culture were established. Sheets of corneal epithelium from eyes of swine and rabbits were obtained by dispase treatment of corneal buttons, and single cells and small clumps were obtained by further dissociation with trypsin...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689008999564
更新日期:1990-09-01 00:00:00
abstract:PURPOSE:To evaluate the immediate effect of 3% diquafosol ophthalmic solution on tear MUC5AC concentration, periodic acid-Schiff (PAS)-positive goblet cells, and tear film stability in normal and keratoconjunctivitis sicca (KCS) rat models. METHODS:Rats were divided into normal and KCS groups. 3% of diquafosol solutio...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2016.1233986
更新日期:2017-05-01 00:00:00
abstract::Purpose: The purpose of this study is to present the study design and one month's preliminary results of a 2-year randomized trial, Combined Atropine with Orthokeratology (AOK), for myopia control study. Methods: Children aged 6 to <11 years and with 1.00-4.00 D myopia were randomly assigned to AOK group or ortho-k al...
journal_title:Current eye research
pub_type: 杂志文章,随机对照试验
doi:10.1080/02713683.2019.1568501
更新日期:2019-06-01 00:00:00
abstract::We studied the effects of a forskolin suspension on intraocular pressure (IOP) in normal albino and pigmented rabbits and in alpha-chymotrypsin induced ocular hypertensive rabbits. Experimental-induced ocular hypertensive rabbits were produced by injecting of alpha-chymotrypsin (167 units) into the posterior chamber o...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688809033195
更新日期:1988-07-01 00:00:00
abstract:PURPOSE:Acanthamoeba keratitis is a sight-threatening corneal infection. It is known that: (i) more amoebae bind to the surface of injured corneas than to the normal corneal surface and (ii) mannose-containing glycoproteins (GPs) possess binding sites for Acanthamoeba. The present study was undertaken to determine whet...
journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:1998-08-01 00:00:00
abstract:PURPOSE:Maintaining mature and viable retinal pigment epithelial cells (RPE) in vitro has proven challenging. Investigating compounds that can promote RPE-viability and maturation is motivated by RPE transplantation research, the quest to understand RPE physiology, and a desire to modulate RPE in pathological states. W...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2020.1809001
更新日期:2020-09-01 00:00:00
abstract:PURPOSE:To study the effect of cataract surgery with intraocular lens (IOL) on frequency doubling perimetry (FDP). METHODS:Patients aged 40 years or above seen at our outpatient clinic with no ocular pathology except for visually significant cataract and visual acuity 6/24 or better were eligible. They underwent FDP b...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680490904278
更新日期:2005-02-01 00:00:00
abstract::To enhance efforts directed at unraveling the role and mechanisms of leukocytes in mediating injury to corneal epithelium, an isolated bovine corneal cup was developed and evaluated. Bovine peripheral leukocytes and lysates were added to the corneal epithelial surface of isolated cornea for various periods after which...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688508999964
更新日期:1985-01-01 00:00:00
abstract:PURPOSE:To examine the effect of elevated extracellular glucose, thus simulating diabetes, on migration, adhesion and proliferation of SV40 transformed human corneal epithelial (HCE) cells. METHODS:HCE cells were maintained in serum supplemented media containing 5 mM, 17.5 mM or 38 mM D-glucose. Cell migration was det...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.17.9.924.5133
更新日期:1998-09-01 00:00:00
abstract:PURPOSE:Klotho genes are expressed in limited tissues and have been found to be a pathogenic factor of age-related diseases in mammals, but their expressions and functions in human eyes are yet to be defined. This study was performed to investigate the expression of Klotho family genes in human ocular tissues and anter...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2016.1259421
更新日期:2017-06-01 00:00:00
abstract:PURPOSE:Deficiency in Cystathionine β-synthase (CBS) leads to an abnormal accumulation of homocysteine and results in classical homocystinuria, a multi-systemic disorder that affects connective tissue, muscles, the central nervous system, and the eyes. However, the genetic players and mechanisms underlying vision alter...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2020.1818262
更新日期:2020-09-07 00:00:00
abstract:PURPOSE:The aim of this study was to examine the functional connectivity between the primary visual cortex and other cortical areas during rest in normal subjects and patients with comitant strabismus using functional magnetic resonance imaging (fMRI). METHODS:A prospective, observational study was conducted. Ten pati...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1540642
更新日期:2019-03-01 00:00:00
abstract:PURPOSE:The effects of several serotonergic agonists on aqueous humor formation (AHF), total outflow facility (OF) and intraocular pressure (IOP) were investigated in living cynomolgus monkeys. METHODS:We determined the effect of a single topical unilateral 300 microg or 3 mg dose of the 5-HT agonists serotonin, 5-car...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.23.2.120.5477
更新日期:2001-08-01 00:00:00
abstract:PURPOSE:This study was designed to investigate whether synthetic small interference RNA (siRNA) targeting VEGF-A could inhibit mouse corneal neovascularization or not. METHODS:First, the effect of synthetic siRNA targeting VEGF-A on the expression of VEGF in human retinal pigment epithelial line ARPE-19 cells was exam...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713681003597230
更新日期:2010-05-01 00:00:00
abstract:PURPOSE:Retinal pigment epithelial (RPE) cell proliferation is an important step in the pathogenesis of ocular diseases such as proliferative retinopathy. Growth factors play a major role in modulating the development and progression of these diseases. The aim of this study was to examine the effects of single growth f...
journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:2000-06-01 00:00:00
abstract:PURPOSE:Taurolidine is a broad-spectrum, non antibiotic antimicrobial agent, not previously tested against the common causes of bacterial keratitis. This study, employing an experimental rabbit model of Staphylococcus aureus keratitis, investigated the effectiveness of topical taurolidine in reducing the number of bact...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680490908733
更新日期:2005-03-01 00:00:00
abstract::Purpose/aim of the study: To observe and analyze the weekly changes in morphology and size of vacuoles, so called Wedl cells, in eyes with posterior subcapsular cataract (PSC). MATERIALS AND METHODS:Thirty-six eyes of 36 patients with pronounced PSC were included in this prospective study. High-resolution retroillumi...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2017.1343852
更新日期:2017-12-01 00:00:00
abstract::Penetrating keratoplasty was the only therapeutic choice for the treatment of corneal endothelial decompensation until the introduction of evolutional endothelial keratoplasties, namely Descemet's stripping automated endothelial keratoplasty (DSAEK) and Descemet's membrane endothelial keratoplasty (DMEK). Although now...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1700529
更新日期:2020-03-01 00:00:00
abstract:PURPOSE:Androgens are thought to play a role in the regulation of the human lacrimal gland. Androgen receptor mRNA has been isolated from human lacrimal tissue; however, it is not known which cell(s) in human lacrimal tissue may contain androgen receptors. This study is an immunohistochemical investigation of the locat...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.18.4.300.5362
更新日期:1999-04-01 00:00:00
abstract::DNA sequence analysis of overlapping shotgun and restriction fragments have revealed the entire sequence of the human lens fiber cell intrinsic membrane protein MP19 gene (also termed MP17, MP18, and MP20). The 8,056 bp MP19 gene contains 5 exons encoding a mature protein of 173 amino acids, which displayed a very hig...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689309033503
更新日期:1993-12-01 00:00:00
abstract::Calmodulin has been shown to perform several important functions in the lens including regulation of the plasma membrane Ca(2+)-ATPase. This study investigated the effects of a variety of different CaM antagonists on rat lens membrane potential, membrane resistance, intracellular Na+ and Ca2+ content and transparency,...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689408995780
更新日期:1994-03-01 00:00:00
abstract:PURPOSE:Mechanical stimulation induces intercellular Ca(2 +) waves in the corneal endothelium. The extent of the wave propagation is dependent on the activity of gap junctions, hemichannels, and ectonucleotidases. To further establish the use of a cell culture model to investigate intercellular communication, in this s...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680902913022
更新日期:2009-06-01 00:00:00
abstract:PURPOSE:To investigate orbital biomechanical properties in patients with thyroid orbitopathy and in age- and gender-matched healthy subjects using the Corvis-ST (CST, Oculus Wetzlar, Germany). METHODS:The CST allows a non-contact tonometry with an ultra-high-speed Scheimpflug device to record the deformation of the co...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2017.1405044
更新日期:2018-03-01 00:00:00
abstract:PURPOSE:The aim of this study was to characterize swelling pressure-thickness, swelling pressure-hydration and hydration-thickness relations of porcine cornea. METHODS:Mechanical compression tests and free swelling experiments were performed on porcine cornea. A rheometer (DHR-2, TA Instruments) with a thermally contr...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2013.809769
更新日期:2013-11-01 00:00:00
abstract:PURPOSE:The fibroblast growth factors (FGFs) are a family of 9 heparin binding proteins which have been proposed to play key roles in angiogenesis. Basic FGF (bFGF), acidic FGF (aFGF) and FGF5 have previously been demonstrated to be expressed in the normal retina and RPE. In this study, the expression of FGF5 was explo...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.4.396.10685
更新日期:1997-04-01 00:00:00
abstract:PURPOSE:Post-surgical macular edema is an important clinical problem resulting from breakdown of the blood-retinal barrier (BRB) after surgery. This study was designed to develop a mouse model of post-surgical BRB breakdown. METHODS:Two 25-gauge needles, one for infusion and one for aspiration, were inserted through t...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680490503769
更新日期:2004-06-01 00:00:00
abstract:PURPOSE:Predicting the toxic potential of compounds to the ocular surface has depended on the Draize test for the past half century. Alternatives to Draize testing have recently been sought for a number of reasons. Stress gene expression has emerged as a means of quantifying cellular reaction and, thus, the toxic poten...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.19.2.115.5325
更新日期:1999-08-01 00:00:00
abstract::In the unsutured partial thickness penetrating wounds of the cornea, the epithelium migrates over the wounded stromal surface prior to the onset of stromal regeneration. To determine the possible affects of the epithelial ingrowth on the organization of the stromal scar tissues, the healing of unsutured and sutured wo...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689508995803
更新日期:1995-09-01 00:00:00
abstract:PURPOSE:To quantify levels of matricellular proteins in aqueous humor samples from acute primary angle closure (APAC) and non-glaucomatous cataract eyes and investigate their correlation with intraocular pressure (IOP) fluctuation. MATERIALS AND METHODS:Aqueous humor samples were collected from 63 eyes including 29 cu...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1449222
更新日期:2018-06-01 00:00:00
abstract:PURPOSE:To investigate the relationship between meibomian gland dysfunction (MGD) and incomplete blinking caused by cranial nerve seven (CN VII) palsy. METHODS:A prospective case series of 60 consecutive patients with unilateral CN VII palsy was evaluated for MGD. According to the House-Brackmann scale, patients were ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2015.1007211
更新日期:2016-01-01 00:00:00