Abstract:
PURPOSE:Deficiency in Cystathionine β-synthase (CBS) leads to an abnormal accumulation of homocysteine and results in classical homocystinuria, a multi-systemic disorder that affects connective tissue, muscles, the central nervous system, and the eyes. However, the genetic players and mechanisms underlying vision alterations in patients with homocystinuria are little understood. MATERIALS AND METHODS:The fruit fly, Drosophila melanogaster, is a useful system to investigate the genetic basis of several human diseases, but no study to date has used Drosophila as model of homocystinuria. Here, we use Drosophila genetic tools to down-regulate CBS expression and evaluate its behavioral response to light. RESULTS:We show that CBS-deficient flies do not display the normal stereotypical behavior of attraction towards a luminous source, known as phototaxis. This behavior cannot be attributed to a motor or olfactory deficiency, but it is most likely related to a lower visual acuity. CBS-deficient flies are overall smaller, but smaller eyes do not explain their lack of phototactic response. CONCLUSIONS:The vision phenotype of CBS knock-down flies is consistent with severe myopia in homocystinuria patients. We propose to use Drosophila as a model to investigate ocular manifestations underlying homocystinuria.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Flores-Flores M,Moreno-García L,Castro-Martínez F,Nahmad Mdoi
10.1080/02713683.2020.1818262subject
Has Abstractpub_date
2020-09-07 00:00:00pages
1-6eissn
0271-3683issn
1460-2202pub_type
杂志文章abstract:PURPOSE:Previous studies revealed an association of tumor necrosis factor super family-15 (TNFSF15) gene polymorphisms with several autoimmune diseases, but their roles in the development of Graves' disease (GD) and Graves' ophthalmopathy (GO) remain unclear. The aim of this study was to explore the correlation between...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1705494
更新日期:2020-07-01 00:00:00
abstract:PURPOSE:To determine the role of matrix metalloproteinase 9 (MMP9) in advanced glycation end-products (AGEs) induced damage to blood-retinal barrier (BRB) and the therapeutic effects of minocycline, an MMP inhibitor, against the BRB damages. METHODS:Forty-eight Sprague Dawley rats were randomly assigned to 3 groups (1...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680802450984
更新日期:2008-11-01 00:00:00
abstract:PURPOSE:To explore the roles of interleukin (IL)-17A in alkali-induced corneal neovascularization (CRNV). METHODS:Induction of CRNV by alkali injury was performed and compared in recombinant mouse IL-17A protein or anti-mouse IL-17A neutralizing antibody (Ab)-treated mice. The expression of IL-17A, IL-17A receptor (IL...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2016.1196705
更新日期:2017-03-01 00:00:00
abstract:PURPOSE:To study changes in the morphometric characteristics of the whole brain visual-related cortex in various stages of primary open angle glaucoma (POAG) in vivo. MATERIALS AND METHODS:Thirty POAG patients (nine early stage cases and 21 advanced-late stage cases) and 30 gender-, education-, and age-matched healthy...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2012.683506
更新日期:2012-09-01 00:00:00
abstract:PURPOSE:Disulfiram (DSF) exhibits a wide variety of biological activities, including an anti-inflammatory action, on which we focused our attention. The aim of the present study was to investigate the effect of oral DSF on endotoxin-induced uveitis (EIU) in rats. METHODS:We investigated its effect upon cellular infilt...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2010.495442
更新日期:2010-10-01 00:00:00
abstract:PURPOSE:NiCl(2) (15 microM) enhances the ERG b-wave amplitude of vertebrate retina, up to 1.5-fold by blocking E/R-type voltage-gated Ca(2+) channels, which is mediated by blocking the release of GABA onto ionotropic GABA-A and GABA-C receptors. In vivo, it is likely that zinc, rather than nickel ions, may be involved ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713680903509410
更新日期:2010-04-01 00:00:00
abstract:PURPOSE:The mechanisms involved in the reported beneficial effects of Calcium dobesilate monohydrate (CaD) for the treatment of diabetic retinopathy (DR) remain to be elucidated. The main aim of the present study is to examine whether CaD prevents early events in the pathogenesis of DR such as neurodegeneration and vas...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2017.1302591
更新日期:2017-09-01 00:00:00
abstract::Recently, all the structural features of non-proliferative diabetic retinopathy have been demonstrated in mice fed 30% galactose for 21-26 months. To determine whether changes in retinal matrix protein mRNA levels occur early in the course of murine diabetes we used a competitive RT-PCR method to quantitate retinal mR...
journal_title:Current eye research
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
abstract:PURPOSE:To assess the rate of achieving optimal metabolic control among subjects with type 2 diabetes, to elucidate the influence of suboptimal control on diabetic retinopathy (DR) and to analyze the risk factors associated with DR in the subjects with suboptimal control. MATERIAL AND METHODS:A population-based, cross...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2012.669005
更新日期:2012-06-01 00:00:00
abstract::The distribution of vasoactive intestinal polypeptide (VIP)- and neurotensin (NT)-like immunoreactivities in the chicken retina was investigated by immunohistochemistry. VIP- and NT-positive cells were found throughout the chicken retina. The majority of them were located in the proximal portion of the inner nuclear l...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688109001736
更新日期:1981-01-01 00:00:00
abstract:PURPOSE:Rhodopsin is a zinc-binding protein. We investigated the effect of low concentrations of zinc on the initial phosphorylation of rhodopsin. METHODS:Dark-adapted bovine rod outer segments (ROS) were incubated with (gamma 32P) ATP and 5 mM magnesium in the presence and absence of micromolar amounts of zinc. The R...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689609017650
更新日期:1996-10-01 00:00:00
abstract:PURPOSE:Tissue inhibitor of metalloproteinase-3 (TIMP-3) is a member of the TIMP family of proteins, playing a significant role in the control of extracellular matrix remodelling. TIMP-3 might play a role in the regulation of retinal neovascularization during progression of diabetic retinopathy. Recently, three novel p...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.27.2.91.15954
更新日期:2003-08-01 00:00:00
abstract:PURPOSE:The present study was aimed to investigate the expression of purinergic P2 receptors in oxygen-induced retinal neovascularization. METHODS:Immunohistochemistry was used to study the expression of purinergic P2Y2 and P2X2 receptors in the neonatal mouse retina during normal vascular development and after oxygen...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680701885470
更新日期:2008-03-01 00:00:00
abstract:PURPOSE:Unstable tear film characterized by shorting of tear break-up time (BUT) is associated with discomfort and dryness in contact lens wearers. The glycocalyx is thought to be crucial in maintaining the wettability and lubrication of the ocular surface. We evaluated the ocular surface glycocalyx in soft contact len...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2014.999948
更新日期:2016-01-01 00:00:00
abstract:PURPOSE:To compare the antiviral effect of a peptide derived from human apolipoprotein E (1% apoEdp) with 1% trifluorothymidine against HSV-1 thymidine kinase (TK)-positive (HSV-1 McKrae) and with 3% foscarnet against HSV-1 TK-negative (KOS background) in the rabbit eye model of acute HSV-1 epithelial keratitis. MATER...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680802647662
更新日期:2009-02-01 00:00:00
abstract::The 'fast PIII' rod mediated photoreceptor response to flashes of monochromatic light has been investigated in isolated retinae of the RCS rat at age 30 days. A loss of both sensitivity and maximum response amplitude were found, with a concurrent slowing of the response kinetics. These observations indicate a rod abno...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409000805
更新日期:1984-09-01 00:00:00
abstract:PURPOSE:We identified a novel mutation in Paired Box gene 6 (PAX6) and characterized its associated clinical features of severe ocular malformation in a Chinese family with congenital aniridia. METHODS:We studied two patients with aniridia from a Chinese family. All patients and noncarriers in this family underwent fu...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2012.688165
更新日期:2012-10-01 00:00:00
abstract:PURPOSE:To investigate the association of nailfold capillaroscopy, heart rate variability (HRV), and clinical characteristics of glaucoma with the plasma matrix metalloproteinase-9 (MMP-9) level in normal-tension glaucoma (NTG). MATERIALS AND METHODS:We conducted a prospective, cross-sectional study on 25 patients wit...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2014.971937
更新日期:2015-01-01 00:00:00
abstract::A major glycoprotein of relative molecular weight of 130,000 daltons of chick lens cell membranes was identified by Concanavalin-A staining and enriched for by Con A-Sepharose chromatography. Using both Con A binding and immunological analyses with a specific polyclonal antibody, the glycoprotein was detected in chick...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688608995176
更新日期:1986-12-01 00:00:00
abstract:PURPOSE:The high prevalence of positive family history of keratoconus (KC) in KC patients is well-known. However, the results regarding the association between family history of KC and disease severity are controversial. The aim of this study was to evaluate the possible association between family history and severity ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2015.1128553
更新日期:2016-11-01 00:00:00
abstract:PURPOSE:The effects of repeated topical administration of the selective beta 2-adrenergic agonist fenoterol on the intraocular pressure and on the adenylyl cyclase activity in ciliary processes in rabbit were examined in order to detect their possible causal relationship. METHODS:Intraocular pressure was measured by p...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.10.1061.9020
更新日期:1997-10-01 00:00:00
abstract:PURPOSE:This study was performed to compare the positive predictive value of peripapillary retinal nerve fiber layer (RNFL) thickness measurements obtained using Cirrus high-definition optical coherence tomography (Cirrus HD-OCT; Carl Zeiss Meditec, Dublin, CA) and spectral OCT/scanning laser ophthalmoscopy (SLO) (OPKO...
journal_title:Current eye research
pub_type: 临床试验,杂志文章
doi:10.3109/02713683.2013.824989
更新日期:2014-01-01 00:00:00
abstract:PURPOSE:In keratoconus corneas, it has been shown that levels of degradative enzymes and transcription factor Sp1 are elevated and those of inhibitors are reduced, especially in the epithelial layer. This study is to determine whether the biochemical abnormalities identified in corneas also exist in conjunctival tissue...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.21.5.886.5538
更新日期:2000-11-01 00:00:00
abstract::Introduction: Metabolic syndrome is a disorder characterized by a constellation of findings including truncal obesity, elevated blood pressure, abnormal cholesterol levels, and high blood glucose. Recent evidence suggests that metabolic syndrome may be associated with increased risk of age-related macular degeneration...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1694156
更新日期:2020-06-01 00:00:00
abstract:PURPOSE:To demonstrate spectroscopic retinal oximetry measurements on arteries and veins in swine using intravitreal illumination. Retinal arterial and venous saturations are measured for a range of inspired O2 levels after pars plana vitrectomy. METHODS:Pars plana vitrectomy and intravitreal manipulations were perfor...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680600760493
更新日期:2006-07-01 00:00:00
abstract::Using an animal model of retinoblastoma in inbred rats and cultured human adenovirus type 12-induced retinoblastoma-like tumor cells (RAO 188), complement-dependent cytotoxicity was determined by measuring release of 3H-uridine labelled RNA. Sera from rats in which tumors did not grow after adenovirus type 12 inoculat...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688109019973
更新日期:1981-01-01 00:00:00
abstract::Samples of aqueous humor from rabbits and humans were examined for growth effects on subconfluent monolayer fibroblast cultures from ocular and extraocular tissues. At a concentration of 20% aqueous by medium volume, both homologous and heterologous aqueous stimulated DNA synthesis in fibroblasts from both species. Th...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688209020019
更新日期:1982-01-01 00:00:00
abstract::Lipid synthesis was investigated in bovine lens in organ culture for 9 hours using 14C acetate as a precursor. The greatest incorporation occurred in phospholipid fraction with phosphatidyl choline being the major phospholipid labeled. Considerable activity was incorporated in the free fatty acid fraction, cholesterol...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688709034889
更新日期:1987-09-01 00:00:00
abstract::It has been shown that illumination of rod outer segment suspension in the presence of photosensitizers (methylene blue lambda greater than or equal to 620 nm; retinal 370 less than or equal to lambda less than or equal to 390 nm) results in chemical modification of the lipid and protein components of the photo-recept...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688209019997
更新日期:1982-01-01 00:00:00
abstract:AIM:To test the feasibility of implanting human anterior lens capsules (HALCs) with porcine corneal endothelial cells (pCEC) in vivo in Göttingen minipigs and at the same time test the suitability of Göttingen minipig as model for endothelial keratoplasty. MATERIALS AND METHODS:Cell-carrier constructs of decellularize...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1706747
更新日期:2020-08-01 00:00:00