Genetic characterization and authentication of Embelia ribes using RAPD-PCR and SCAR marker.

Abstract:

:Embelia ribes Burm. f. (Myrsinaceae) is one of the important plants used in Indian traditional medicine. RAPD-PCR analysis was performed to obtain species-specific DNA fragments. A band of 906 bp that was specific to Embelia ribes irrespective of the geographical source was obtained using the random decamer primer OPF 05. SCAR primers ER 1 (27 mer) and ER 2 (26 mer) were designed from the sequence of the RAPD marker which yielded an expected amplicon of 594 bp with the Embelia ribes DNA only. This methodology can be used for species identification of genuine Embelia ribes and to distinguish it from common substitutes and adulterants. BLAST: basic local alignment search tool ER 1: Embelia ribes forward primer ER 2: Embelia ribes reverse primer RAPD-PCR: random amplification of polymorphic DNA polymerase chain reaction SCAR: sequence characterized amplified region.

journal_name

Planta Med

journal_title

Planta medica

authors

Devaiah KM,Venkatasubramanian P

doi

10.1055/s-2008-1034279

subject

Has Abstract

pub_date

2008-02-01 00:00:00

pages

194-6

issue

2

eissn

0032-0943

issn

1439-0221

journal_volume

74

pub_type

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